Cryopreservation of immature maize embryos after freeze-hardening in the ear and in vitro

We have studied cryopreservation of immature maize embyros. Common cyroprotective treatments, i.e., pretreatment with dimethysulfoxide and glycerol, followed by slow-freezing, were not successful. By freeze-hardening, we obtained a high percentage of embryos which survived cryopreservation. The free...

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Veröffentlicht in:	Plant science (Limerick) 1989, Vol.60 (1), p.129-136
Hauptverfasser:	Delvallée, I., Guillaud, J., Beckert, M., Dumas, C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions Biological and medical sciences Breeding schemes. Varia cryopreservation embryo freeze-hardening Fundamental and applied biological sciences. Psychology Genetics Genetics and breeding of economic plants Life Sciences Plant breeding: fundamental aspects and methodology Plant physiology and development Plants genetics Water and solutes. Absorption, translocation and permeability water stress Zea mays
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