Expression of a mouse metallothionein gene in transgenic plant tissues
Three gene constructions based on a mouse metallothionein I gene ( mMT-I) were introduced into tobacco using an Ri plasmid vector system to test the effectiveness of animal gene regulatory signals in plant cells. No transcription from the native mouse gene was observed. In plant cells bearing chimer...
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| Veröffentlicht in: | Gene 1989-04, Vol.77 (1), p.133-140 |
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| creator | Pautot, Véronique Brzezinski, Ryszard Tepfer, Mark |
| description | Three gene constructions based on a mouse metallothionein I gene (
mMT-I) were introduced into tobacco using an Ri plasmid vector system to test the effectiveness of animal gene regulatory signals in plant cells. No transcription from the native mouse gene was observed. In plant cells bearing chimeric
mMT-I genes in which transcription was driven by the nopaline synthase promoter, neither polyadenylation nor splicing of
mMT-I pre-mRNA was observed. Detailed comparisons of
mMT-I sequences with those of known plant genes were carried out; slight differences in regions of known consensus sequences may be at least partly responsible for the non-recognition of
mMT-I gene regulatory signals in plant cells, though other as yet unidentified, potentially necessary sequences may also be involved. |
| doi_str_mv | 10.1016/0378-1119(89)90367-3 |
| format | Article |
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mMT-I) were introduced into tobacco using an Ri plasmid vector system to test the effectiveness of animal gene regulatory signals in plant cells. No transcription from the native mouse gene was observed. In plant cells bearing chimeric
mMT-I genes in which transcription was driven by the nopaline synthase promoter, neither polyadenylation nor splicing of
mMT-I pre-mRNA was observed. Detailed comparisons of
mMT-I sequences with those of known plant genes were carried out; slight differences in regions of known consensus sequences may be at least partly responsible for the non-recognition of
mMT-I gene regulatory signals in plant cells, though other as yet unidentified, potentially necessary sequences may also be involved.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(89)90367-3</identifier><identifier>PMID: 2744484</identifier><identifier>CODEN: GENED6</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Agronomy. Soil science and plant productions ; Animals ; Base Sequence ; Biological and medical sciences ; Biotechnology ; Cloning, Molecular ; DNA ; DNA, Recombinant ; exon ; Exons ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; Genetic engineering ; Genetic engineering applications ; Genetic technics ; Genetic Vectors ; Genetics ; Genetics and breeding of economic plants ; intron ; Life Sciences ; Metallothionein - biosynthesis ; Metallothionein - genetics ; Methods. Procedures. Technologies ; Mice ; Molecular Sequence Data ; Nicotiana - genetics ; Plant breeding: fundamental aspects and methodology ; Plants, Toxic ; polyadenylation ; pre-mRNA splicing ; Promoter Regions, Genetic ; Recombinant DNA ; regulatory signals ; Ri plasmid ; RNA maturation ; RNA Splicing ; Sequence Homology, Nucleic Acid ; Species Specificity ; transcription ; Transformation, Genetic ; vectors</subject><ispartof>Gene, 1989-04, Vol.77 (1), p.133-140</ispartof><rights>1989</rights><rights>1990 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-e996cf90baaaaedfa17fc57bacc5f56f6a10238fb3fbd95b863e6f9334eb362a3</citedby><cites>FETCH-LOGICAL-c451t-e996cf90baaaaedfa17fc57bacc5f56f6a10238fb3fbd95b863e6f9334eb362a3</cites><orcidid>0000-0002-0059-0050</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(89)90367-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6671854$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2744484$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02716809$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Pautot, Véronique</creatorcontrib><creatorcontrib>Brzezinski, Ryszard</creatorcontrib><creatorcontrib>Tepfer, Mark</creatorcontrib><title>Expression of a mouse metallothionein gene in transgenic plant tissues</title><title>Gene</title><addtitle>Gene</addtitle><description>Three gene constructions based on a mouse metallothionein I gene (
mMT-I) were introduced into tobacco using an Ri plasmid vector system to test the effectiveness of animal gene regulatory signals in plant cells. No transcription from the native mouse gene was observed. In plant cells bearing chimeric
mMT-I genes in which transcription was driven by the nopaline synthase promoter, neither polyadenylation nor splicing of
mMT-I pre-mRNA was observed. Detailed comparisons of
mMT-I sequences with those of known plant genes were carried out; slight differences in regions of known consensus sequences may be at least partly responsible for the non-recognition of
mMT-I gene regulatory signals in plant cells, though other as yet unidentified, potentially necessary sequences may also be involved.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cloning, Molecular</subject><subject>DNA</subject><subject>DNA, Recombinant</subject><subject>exon</subject><subject>Exons</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>Genetic engineering</subject><subject>Genetic engineering applications</subject><subject>Genetic technics</subject><subject>Genetic Vectors</subject><subject>Genetics</subject><subject>Genetics and breeding of economic plants</subject><subject>intron</subject><subject>Life Sciences</subject><subject>Metallothionein - biosynthesis</subject><subject>Metallothionein - genetics</subject><subject>Methods. Procedures. Technologies</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Nicotiana - genetics</subject><subject>Plant breeding: fundamental aspects and methodology</subject><subject>Plants, Toxic</subject><subject>polyadenylation</subject><subject>pre-mRNA splicing</subject><subject>Promoter Regions, Genetic</subject><subject>Recombinant DNA</subject><subject>regulatory signals</subject><subject>Ri plasmid</subject><subject>RNA maturation</subject><subject>RNA Splicing</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Species Specificity</subject><subject>transcription</subject><subject>Transformation, Genetic</subject><subject>vectors</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLAzEQgIMoWqv_QGEPIvawmmw2r4tQxBcUvOg5ZLMTG9lHTbai_96sLT06lyEz3wyTD6Ezgq8JJvwGUyFzQoi6kmqmMOUip3toQqRQOcZU7qPJDjlCxzF-4BSMFYfosBBlWcpygh7uv1cBYvR9l_UuM1nbryNkLQymafphmergu-wdOshSHoLpYnp4m60a0w3Z4GNcQzxBB840EU63eYreHu5f757yxcvj8918kduSkSEHpbh1ClcmBdTOEOEsE5WxljnGHTcEF1S6irqqVqySnAJ3itISKsoLQ6dottm7NI1eBd-a8KN74_XTfKHHGi4E4RKrL5LYyw27Cv1nunHQrY8WmnQ3pE9qwgTnnPAElhvQhj7GAG63mWA9qtajRz161FLpP9WaprHz7f511UK9G9q6Tf2Lbd9EaxqX1Fkfdxjngkg2YrcbDJK3Lw9BR-uhs1D7AHbQde__v-MXmIGaqA</recordid><startdate>19890415</startdate><enddate>19890415</enddate><creator>Pautot, Véronique</creator><creator>Brzezinski, Ryszard</creator><creator>Tepfer, Mark</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-0059-0050</orcidid></search><sort><creationdate>19890415</creationdate><title>Expression of a mouse metallothionein gene in transgenic plant tissues</title><author>Pautot, Véronique ; Brzezinski, Ryszard ; Tepfer, Mark</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-e996cf90baaaaedfa17fc57bacc5f56f6a10238fb3fbd95b863e6f9334eb362a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cloning, Molecular</topic><topic>DNA</topic><topic>DNA, Recombinant</topic><topic>exon</topic><topic>Exons</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>Genetic engineering</topic><topic>Genetic engineering applications</topic><topic>Genetic technics</topic><topic>Genetic Vectors</topic><topic>Genetics</topic><topic>Genetics and breeding of economic plants</topic><topic>intron</topic><topic>Life Sciences</topic><topic>Metallothionein - biosynthesis</topic><topic>Metallothionein - genetics</topic><topic>Methods. Procedures. Technologies</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Nicotiana - genetics</topic><topic>Plant breeding: fundamental aspects and methodology</topic><topic>Plants, Toxic</topic><topic>polyadenylation</topic><topic>pre-mRNA splicing</topic><topic>Promoter Regions, Genetic</topic><topic>Recombinant DNA</topic><topic>regulatory signals</topic><topic>Ri plasmid</topic><topic>RNA maturation</topic><topic>RNA Splicing</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Species Specificity</topic><topic>transcription</topic><topic>Transformation, Genetic</topic><topic>vectors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pautot, Véronique</creatorcontrib><creatorcontrib>Brzezinski, Ryszard</creatorcontrib><creatorcontrib>Tepfer, Mark</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pautot, Véronique</au><au>Brzezinski, Ryszard</au><au>Tepfer, Mark</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of a mouse metallothionein gene in transgenic plant tissues</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1989-04-15</date><risdate>1989</risdate><volume>77</volume><issue>1</issue><spage>133</spage><epage>140</epage><pages>133-140</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><coden>GENED6</coden><abstract>Three gene constructions based on a mouse metallothionein I gene (
mMT-I) were introduced into tobacco using an Ri plasmid vector system to test the effectiveness of animal gene regulatory signals in plant cells. No transcription from the native mouse gene was observed. In plant cells bearing chimeric
mMT-I genes in which transcription was driven by the nopaline synthase promoter, neither polyadenylation nor splicing of
mMT-I pre-mRNA was observed. Detailed comparisons of
mMT-I sequences with those of known plant genes were carried out; slight differences in regions of known consensus sequences may be at least partly responsible for the non-recognition of
mMT-I gene regulatory signals in plant cells, though other as yet unidentified, potentially necessary sequences may also be involved.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>2744484</pmid><doi>10.1016/0378-1119(89)90367-3</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-0059-0050</orcidid></addata></record> |
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| ispartof | Gene, 1989-04, Vol.77 (1), p.133-140 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Agronomy. Soil science and plant productions Animals Base Sequence Biological and medical sciences Biotechnology Cloning, Molecular DNA DNA, Recombinant exon Exons Fundamental and applied biological sciences. Psychology Gene Expression Regulation Genetic engineering Genetic engineering applications Genetic technics Genetic Vectors Genetics Genetics and breeding of economic plants intron Life Sciences Metallothionein - biosynthesis Metallothionein - genetics Methods. Procedures. Technologies Mice Molecular Sequence Data Nicotiana - genetics Plant breeding: fundamental aspects and methodology Plants, Toxic polyadenylation pre-mRNA splicing Promoter Regions, Genetic Recombinant DNA regulatory signals Ri plasmid RNA maturation RNA Splicing Sequence Homology, Nucleic Acid Species Specificity transcription Transformation, Genetic vectors |
| title | Expression of a mouse metallothionein gene in transgenic plant tissues |
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