Interference between Rhizobium meliloti and Rhizobium trifolii nodulation genes: genetic basis of R. meliloti dominance
Transfer of an IncP plasmid carrying the Rhizobium meliloti nodFE, nodG, and nodH genes to Rhizobium trifolii enabled R. trifolii to nodulate alfalfa (Medicago sativa), the normal host of R. meliloti. Using transposon Tn5-linked mutations and in vitro-constructed deletions of the R. meliloti nodFE,...
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| Veröffentlicht in: | Journal of Bacteriology 1988-12, Vol.170 (12), p.5718-5727 |
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| creator | DEBELLE, F MAILLET, F VASSE, J ROSENBERG, C DE BILLY, F TRUCHET, G DENARIE, J AUSUBEL, F. M |
| description | Transfer of an IncP plasmid carrying the Rhizobium meliloti nodFE, nodG, and nodH genes to Rhizobium trifolii enabled R. trifolii to nodulate alfalfa (Medicago sativa), the normal host of R. meliloti. Using transposon Tn5-linked mutations and in vitro-constructed deletions of the R. meliloti nodFE, nodG, and nodH genes, we showed that R. meliloti nodH was required for R. trifolii to elicit both root hair curling and nodule initiation on alfalfa and that nodH, nodFE, and nodG were required for R. trifolii to elicit infection threads in alfalfa root hairs. Interestingly, the transfer of the R. meliloti nodFE, nodG, and nodH genes to R. trifolii prevented R. trifolii from infecting and nodulating its normal host, white clover (Trifolium repens). Experiments with the mutated R. meliloti nodH, nodF, nodE, and nodG genes demonstrated that nodH, nodF, nodE, and possibly have an additive effect in blocking infection and nodulation of clover. |
| doi_str_mv | 10.1128/jb.170.12.5718-5727.1988 |
| format | Article |
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M</creator><creatorcontrib>DEBELLE, F ; MAILLET, F ; VASSE, J ; ROSENBERG, C ; DE BILLY, F ; TRUCHET, G ; DENARIE, J ; AUSUBEL, F. M</creatorcontrib><description>Transfer of an IncP plasmid carrying the Rhizobium meliloti nodFE, nodG, and nodH genes to Rhizobium trifolii enabled R. trifolii to nodulate alfalfa (Medicago sativa), the normal host of R. meliloti. Using transposon Tn5-linked mutations and in vitro-constructed deletions of the R. meliloti nodFE, nodG, and nodH genes, we showed that R. meliloti nodH was required for R. trifolii to elicit both root hair curling and nodule initiation on alfalfa and that nodH, nodFE, and nodG were required for R. trifolii to elicit infection threads in alfalfa root hairs. Interestingly, the transfer of the R. meliloti nodFE, nodG, and nodH genes to R. trifolii prevented R. trifolii from infecting and nodulating its normal host, white clover (Trifolium repens). Experiments with the mutated R. meliloti nodH, nodF, nodE, and nodG genes demonstrated that nodH, nodF, nodE, and possibly have an additive effect in blocking infection and nodulation of clover.</description><identifier>ISSN: 0021-9193</identifier><identifier>EISSN: 1098-5530</identifier><identifier>EISSN: 1067-8832</identifier><identifier>DOI: 10.1128/jb.170.12.5718-5727.1988</identifier><identifier>PMID: 2848012</identifier><identifier>CODEN: JOBAAY</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Agronomy. Soil science and plant productions ; Bacteriology ; Biological and medical sciences ; Chromosome Deletion ; DNA Transposable Elements ; Economic plant physiology ; Escherichia coli - genetics ; formation de nodosites ; Fundamental and applied biological sciences. Psychology ; gene ; genes ; Genes, Bacterial ; Genotype ; infectivity ; Kinetics ; Life Sciences ; medicago sativa ; Microbiology ; Microbiology and Parasitology ; Nitrogen Fixation - genetics ; nodulacion ; parasitism ; parasitisme ; parasitismo ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Plants - microbiology ; Plasmids ; poder infeccioso ; pouvoir infectant ; Restriction Mapping ; rhizobium ; Rhizobium - genetics ; Rhizobium meliloti ; Rhizobium trifolii ; root nodulation ; Species Specificity ; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...) ; trifolium repens</subject><ispartof>Journal of Bacteriology, 1988-12, Vol.170 (12), p.5718-5727</ispartof><rights>1989 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c679t-1400d7c168a8f15e2dacd5cac8af1b0fd258896147d42624f90bbfbcf281d23a3</citedby><cites>FETCH-LOGICAL-c679t-1400d7c168a8f15e2dacd5cac8af1b0fd258896147d42624f90bbfbcf281d23a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC211674/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC211674/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7248663$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2848012$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02716457$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>DEBELLE, F</creatorcontrib><creatorcontrib>MAILLET, F</creatorcontrib><creatorcontrib>VASSE, J</creatorcontrib><creatorcontrib>ROSENBERG, C</creatorcontrib><creatorcontrib>DE BILLY, F</creatorcontrib><creatorcontrib>TRUCHET, G</creatorcontrib><creatorcontrib>DENARIE, J</creatorcontrib><creatorcontrib>AUSUBEL, F. M</creatorcontrib><title>Interference between Rhizobium meliloti and Rhizobium trifolii nodulation genes: genetic basis of R. meliloti dominance</title><title>Journal of Bacteriology</title><addtitle>J Bacteriol</addtitle><description>Transfer of an IncP plasmid carrying the Rhizobium meliloti nodFE, nodG, and nodH genes to Rhizobium trifolii enabled R. trifolii to nodulate alfalfa (Medicago sativa), the normal host of R. meliloti. Using transposon Tn5-linked mutations and in vitro-constructed deletions of the R. meliloti nodFE, nodG, and nodH genes, we showed that R. meliloti nodH was required for R. trifolii to elicit both root hair curling and nodule initiation on alfalfa and that nodH, nodFE, and nodG were required for R. trifolii to elicit infection threads in alfalfa root hairs. Interestingly, the transfer of the R. meliloti nodFE, nodG, and nodH genes to R. trifolii prevented R. trifolii from infecting and nodulating its normal host, white clover (Trifolium repens). Experiments with the mutated R. meliloti nodH, nodF, nodE, and nodG genes demonstrated that nodH, nodF, nodE, and possibly have an additive effect in blocking infection and nodulation of clover.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Chromosome Deletion</subject><subject>DNA Transposable Elements</subject><subject>Economic plant physiology</subject><subject>Escherichia coli - genetics</subject><subject>formation de nodosites</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene</subject><subject>genes</subject><subject>Genes, Bacterial</subject><subject>Genotype</subject><subject>infectivity</subject><subject>Kinetics</subject><subject>Life Sciences</subject><subject>medicago sativa</subject><subject>Microbiology</subject><subject>Microbiology and Parasitology</subject><subject>Nitrogen Fixation - genetics</subject><subject>nodulacion</subject><subject>parasitism</subject><subject>parasitisme</subject><subject>parasitismo</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Plants - microbiology</subject><subject>Plasmids</subject><subject>poder infeccioso</subject><subject>pouvoir infectant</subject><subject>Restriction Mapping</subject><subject>rhizobium</subject><subject>Rhizobium - genetics</subject><subject>Rhizobium meliloti</subject><subject>Rhizobium trifolii</subject><subject>root nodulation</subject><subject>Species Specificity</subject><subject>Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)</subject><subject>trifolium repens</subject><issn>0021-9193</issn><issn>1098-5530</issn><issn>1067-8832</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkl1rFDEUhgdR6rb6E5RBRPBi1pxMMkkEL0pRW1gQqr0OmUyym2UmqclMF_31zbjLWnvjVcI5z3s-OG9RlICWAJh_2LZLYPmPl5QBryjDbAmC8yfFApDIAVqjp8UCIQyVAFE_L05T2iIEhFB8UpxgTjgCvCh2V3400ZpovDZla8adMb683rjfoXXTUA6md30YXal89yA8RmdD71zpQzf1anTBl2vjTfr45xmdLluVXCqDLa-Xf6t0YXBe5VYvimdW9cm8PLxnxc2Xzz8uLqvVt69XF-erSjdMjBUQhDqmoeGKW6AGd0p3VCvNlYUW2Q5TzkUDhHUEN5hYgdrWttpiDh2uVX1WfNrXvZ3awXTa-DGqXt5GN6j4Swbl5L8Z7zZyHe4kBmgYyfr3e_3mkeryfCXnGMIMGkLZHWT23aFXDD8nk0Y5uKRN3ytvwpQk45SAwOi_IFAQRKAmg3wP6hhSisYeRwAkZyPIbSuzESRgORtBzkaQsxGy9NXDxY_Cw-Vz_u0hr5JWvY35Ki4dMYYJb5o6Y28O-7v1ZueikSoNj7pm6PUesipItY65zs33PIVAUCOaF74H8vjS3w</recordid><startdate>19881201</startdate><enddate>19881201</enddate><creator>DEBELLE, F</creator><creator>MAILLET, F</creator><creator>VASSE, J</creator><creator>ROSENBERG, C</creator><creator>DE BILLY, F</creator><creator>TRUCHET, G</creator><creator>DENARIE, J</creator><creator>AUSUBEL, F. 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Psychology</topic><topic>gene</topic><topic>genes</topic><topic>Genes, Bacterial</topic><topic>Genotype</topic><topic>infectivity</topic><topic>Kinetics</topic><topic>Life Sciences</topic><topic>medicago sativa</topic><topic>Microbiology</topic><topic>Microbiology and Parasitology</topic><topic>Nitrogen Fixation - genetics</topic><topic>nodulacion</topic><topic>parasitism</topic><topic>parasitisme</topic><topic>parasitismo</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Plants - microbiology</topic><topic>Plasmids</topic><topic>poder infeccioso</topic><topic>pouvoir infectant</topic><topic>Restriction Mapping</topic><topic>rhizobium</topic><topic>Rhizobium - genetics</topic><topic>Rhizobium meliloti</topic><topic>Rhizobium trifolii</topic><topic>root nodulation</topic><topic>Species Specificity</topic><topic>Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)</topic><topic>trifolium repens</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DEBELLE, F</creatorcontrib><creatorcontrib>MAILLET, F</creatorcontrib><creatorcontrib>VASSE, J</creatorcontrib><creatorcontrib>ROSENBERG, C</creatorcontrib><creatorcontrib>DE BILLY, F</creatorcontrib><creatorcontrib>TRUCHET, G</creatorcontrib><creatorcontrib>DENARIE, J</creatorcontrib><creatorcontrib>AUSUBEL, F. 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M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interference between Rhizobium meliloti and Rhizobium trifolii nodulation genes: genetic basis of R. meliloti dominance</atitle><jtitle>Journal of Bacteriology</jtitle><addtitle>J Bacteriol</addtitle><date>1988-12-01</date><risdate>1988</risdate><volume>170</volume><issue>12</issue><spage>5718</spage><epage>5727</epage><pages>5718-5727</pages><issn>0021-9193</issn><eissn>1098-5530</eissn><eissn>1067-8832</eissn><coden>JOBAAY</coden><abstract>Transfer of an IncP plasmid carrying the Rhizobium meliloti nodFE, nodG, and nodH genes to Rhizobium trifolii enabled R. trifolii to nodulate alfalfa (Medicago sativa), the normal host of R. meliloti. Using transposon Tn5-linked mutations and in vitro-constructed deletions of the R. meliloti nodFE, nodG, and nodH genes, we showed that R. meliloti nodH was required for R. trifolii to elicit both root hair curling and nodule initiation on alfalfa and that nodH, nodFE, and nodG were required for R. trifolii to elicit infection threads in alfalfa root hairs. Interestingly, the transfer of the R. meliloti nodFE, nodG, and nodH genes to R. trifolii prevented R. trifolii from infecting and nodulating its normal host, white clover (Trifolium repens). Experiments with the mutated R. meliloti nodH, nodF, nodE, and nodG genes demonstrated that nodH, nodF, nodE, and possibly have an additive effect in blocking infection and nodulation of clover.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>2848012</pmid><doi>10.1128/jb.170.12.5718-5727.1988</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 0021-9193 1098-5530 1067-8832 |
| language | eng |
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| subjects | Agronomy. Soil science and plant productions Bacteriology Biological and medical sciences Chromosome Deletion DNA Transposable Elements Economic plant physiology Escherichia coli - genetics formation de nodosites Fundamental and applied biological sciences. Psychology gene genes Genes, Bacterial Genotype infectivity Kinetics Life Sciences medicago sativa Microbiology Microbiology and Parasitology Nitrogen Fixation - genetics nodulacion parasitism parasitisme parasitismo Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Plants - microbiology Plasmids poder infeccioso pouvoir infectant Restriction Mapping rhizobium Rhizobium - genetics Rhizobium meliloti Rhizobium trifolii root nodulation Species Specificity Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...) trifolium repens |
| title | Interference between Rhizobium meliloti and Rhizobium trifolii nodulation genes: genetic basis of R. meliloti dominance |
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