Bovine αs2 -casein D is generated by exon VIII skipping
Bovine α s2 -casein D (CasD) differs from the common type A by the deletion of a stretch of 9 amino acids (aa) starting at a position not precisely known, either at aa 50, 51, or 52. The sequence of cloned PCR-amplified genomic DNA from three homozygous cows, two unrelated females carrying the CasD...
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| Veröffentlicht in: | Gene 1993-06, Vol.128 (2), p.289-293 |
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| creator | Bouniol, Christine Printz, Christiane Mercier, Jean-Claude |
| description | Bovine
α
s2
-casein D (CasD) differs from the common type A by the deletion of a stretch of 9 amino acids (aa) starting at a position not precisely known, either at aa 50, 51, or 52. The sequence of cloned PCR-amplified genomic DNA from three homozygous cows, two unrelated females carrying the
CasD allele and one carrying the
CasA allele, did not reveal any deletion and showed two identical nucleotide (nt) substitutions in the 1.7-kb region of both
CasD alleles encompassing codons 43–75 in the cDNA encoding
α
s2
-CasA. This strongly suggests that the deleted bovine
α
s2
-CasD arises from skipping the 27-nt exon, now identified as exon VIII, which encodes aa 51–59 of
α
s2
-CasA. The G→T transversion (allele A→D) affecting the last nt of exon VIII, i.e., the 5' consensus splicing site, might be responsible for the altered splicing of the primary transcript of
α
s2
-
CasD. |
| doi_str_mv | 10.1016/0378-1119(93)90577-P |
| format | Article |
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α
s2
-casein D (CasD) differs from the common type A by the deletion of a stretch of 9 amino acids (aa) starting at a position not precisely known, either at aa 50, 51, or 52. The sequence of cloned PCR-amplified genomic DNA from three homozygous cows, two unrelated females carrying the
CasD allele and one carrying the
CasA allele, did not reveal any deletion and showed two identical nucleotide (nt) substitutions in the 1.7-kb region of both
CasD alleles encompassing codons 43–75 in the cDNA encoding
α
s2
-CasA. This strongly suggests that the deleted bovine
α
s2
-CasD arises from skipping the 27-nt exon, now identified as exon VIII, which encodes aa 51–59 of
α
s2
-CasA. The G→T transversion (allele A→D) affecting the last nt of exon VIII, i.e., the 5' consensus splicing site, might be responsible for the altered splicing of the primary transcript of
α
s2
-
CasD.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(93)90577-P</identifier><identifier>PMID: 8514196</identifier><identifier>CODEN: GENED6</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Alleles ; alphaS1-casein ; alternative splicing ; amino acid sequences ; Animals ; Base Sequence ; Biological and medical sciences ; casa allele ; casd allele ; Caseins - genetics ; Cattle ; complementary DNA ; Consensus Sequence ; deletion ; DNA Mutational Analysis ; Exons ; Female ; Fundamental and applied biological sciences. Psychology ; genbank/x68971 ; genbank/x68972 ; Genes. Genome ; Genetics ; Life Sciences ; milk protein ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; nucleotide sequences ; Point Mutation ; Polymerase Chain Reaction ; RNA Precursors - genetics ; RNA Splicing ; RNA, Messenger - genetics ; Sequence Deletion</subject><ispartof>Gene, 1993-06, Vol.128 (2), p.289-293</ispartof><rights>1993</rights><rights>1993 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-fa90c8aa00ae108009dbbaf538e682e0c339dfd1f3f3c2e335e5663b83439f1f3</citedby><cites>FETCH-LOGICAL-c359t-fa90c8aa00ae108009dbbaf538e682e0c339dfd1f3f3c2e335e5663b83439f1f3</cites><orcidid>0000-0002-8966-9522</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(93)90577-P$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4832448$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8514196$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02714679$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Bouniol, Christine</creatorcontrib><creatorcontrib>Printz, Christiane</creatorcontrib><creatorcontrib>Mercier, Jean-Claude</creatorcontrib><title>Bovine αs2 -casein D is generated by exon VIII skipping</title><title>Gene</title><addtitle>Gene</addtitle><description>Bovine
α
s2
-casein D (CasD) differs from the common type A by the deletion of a stretch of 9 amino acids (aa) starting at a position not precisely known, either at aa 50, 51, or 52. The sequence of cloned PCR-amplified genomic DNA from three homozygous cows, two unrelated females carrying the
CasD allele and one carrying the
CasA allele, did not reveal any deletion and showed two identical nucleotide (nt) substitutions in the 1.7-kb region of both
CasD alleles encompassing codons 43–75 in the cDNA encoding
α
s2
-CasA. This strongly suggests that the deleted bovine
α
s2
-CasD arises from skipping the 27-nt exon, now identified as exon VIII, which encodes aa 51–59 of
α
s2
-CasA. The G→T transversion (allele A→D) affecting the last nt of exon VIII, i.e., the 5' consensus splicing site, might be responsible for the altered splicing of the primary transcript of
α
s2
-
CasD.</description><subject>Alleles</subject><subject>alphaS1-casein</subject><subject>alternative splicing</subject><subject>amino acid sequences</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>casa allele</subject><subject>casd allele</subject><subject>Caseins - genetics</subject><subject>Cattle</subject><subject>complementary DNA</subject><subject>Consensus Sequence</subject><subject>deletion</subject><subject>DNA Mutational Analysis</subject><subject>Exons</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genbank/x68971</subject><subject>genbank/x68972</subject><subject>Genes. Genome</subject><subject>Genetics</subject><subject>Life Sciences</subject><subject>milk protein</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequences</subject><subject>Point Mutation</subject><subject>Polymerase Chain Reaction</subject><subject>RNA Precursors - genetics</subject><subject>RNA Splicing</subject><subject>RNA, Messenger - genetics</subject><subject>Sequence Deletion</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM9uEzEQhy0EKiHwBiB8QIgeFsb2em1fkEr500iRqATlanm942DY7KZ2EtHH4kV4Jhw2ypE5eKSZb36yPkKeMnjNgDVvQChdMcbMKyPODUilqut7ZMa0MhWA0PfJ7IQ8JI9y_gGlpORn5ExLVjPTzIh-N-7jgPTP78xp5V3GOND3NGa6wgGT22JH2zuKv8aBflssFjT_jJtNHFaPyYPg-oxPjn1Obj5--Hp5VS0_f1pcXiwrL6TZVsEZ8No5AIcMNIDp2tYFKTQ2miN4IUwXOhZEEJ6jEBJl04hWi1qYUMZzcj7lfne93aS4dunOji7aq4ulPcyAK1Y3yuxZYV9O7CaNtzvMW7uO2WPfuwHHXbZKKm0kbwpYT6BPY84JwymZgT3ItQdz9mDOGmH_ybXX5ezZMX_XrrE7HR1tlv2L495l7_qQ3OBjPmG1Frwuz5w8n7DgRutWqSA3XzgwAUzpGjgvxNuJwGJ2HzHZ7CMOHruY0G9tN8b___QvSzOcQw</recordid><startdate>19930630</startdate><enddate>19930630</enddate><creator>Bouniol, Christine</creator><creator>Printz, Christiane</creator><creator>Mercier, Jean-Claude</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-8966-9522</orcidid></search><sort><creationdate>19930630</creationdate><title>Bovine αs2 -casein D is generated by exon VIII skipping</title><author>Bouniol, Christine ; Printz, Christiane ; Mercier, Jean-Claude</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-fa90c8aa00ae108009dbbaf538e682e0c339dfd1f3f3c2e335e5663b83439f1f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Alleles</topic><topic>alphaS1-casein</topic><topic>alternative splicing</topic><topic>amino acid sequences</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>casa allele</topic><topic>casd allele</topic><topic>Caseins - genetics</topic><topic>Cattle</topic><topic>complementary DNA</topic><topic>Consensus Sequence</topic><topic>deletion</topic><topic>DNA Mutational Analysis</topic><topic>Exons</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genbank/x68971</topic><topic>genbank/x68972</topic><topic>Genes. Genome</topic><topic>Genetics</topic><topic>Life Sciences</topic><topic>milk protein</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequences</topic><topic>Point Mutation</topic><topic>Polymerase Chain Reaction</topic><topic>RNA Precursors - genetics</topic><topic>RNA Splicing</topic><topic>RNA, Messenger - genetics</topic><topic>Sequence Deletion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bouniol, Christine</creatorcontrib><creatorcontrib>Printz, Christiane</creatorcontrib><creatorcontrib>Mercier, Jean-Claude</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bouniol, Christine</au><au>Printz, Christiane</au><au>Mercier, Jean-Claude</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Bovine αs2 -casein D is generated by exon VIII skipping</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1993-06-30</date><risdate>1993</risdate><volume>128</volume><issue>2</issue><spage>289</spage><epage>293</epage><pages>289-293</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><coden>GENED6</coden><abstract>Bovine
α
s2
-casein D (CasD) differs from the common type A by the deletion of a stretch of 9 amino acids (aa) starting at a position not precisely known, either at aa 50, 51, or 52. The sequence of cloned PCR-amplified genomic DNA from three homozygous cows, two unrelated females carrying the
CasD allele and one carrying the
CasA allele, did not reveal any deletion and showed two identical nucleotide (nt) substitutions in the 1.7-kb region of both
CasD alleles encompassing codons 43–75 in the cDNA encoding
α
s2
-CasA. This strongly suggests that the deleted bovine
α
s2
-CasD arises from skipping the 27-nt exon, now identified as exon VIII, which encodes aa 51–59 of
α
s2
-CasA. The G→T transversion (allele A→D) affecting the last nt of exon VIII, i.e., the 5' consensus splicing site, might be responsible for the altered splicing of the primary transcript of
α
s2
-
CasD.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>8514196</pmid><doi>10.1016/0378-1119(93)90577-P</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-8966-9522</orcidid></addata></record> |
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| subjects | Alleles alphaS1-casein alternative splicing amino acid sequences Animals Base Sequence Biological and medical sciences casa allele casd allele Caseins - genetics Cattle complementary DNA Consensus Sequence deletion DNA Mutational Analysis Exons Female Fundamental and applied biological sciences. Psychology genbank/x68971 genbank/x68972 Genes. Genome Genetics Life Sciences milk protein Molecular and cellular biology Molecular genetics Molecular Sequence Data nucleotide sequences Point Mutation Polymerase Chain Reaction RNA Precursors - genetics RNA Splicing RNA, Messenger - genetics Sequence Deletion |
| title | Bovine αs2 -casein D is generated by exon VIII skipping |
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