Immunological characterization of a 17‐kDa antigen from Cryptosporidium parvum recognized early by mucosal IgA antibodies
Cryptosporidium parvum antigens were characterized by immunoblot analysis of sera and intestinal secretions of BALB/c mice orally infected with 105 oocysts. A major band at 17 kDa under non‐reduced conditions and at 18 kDa under reduced conditions was recognized by anti‐C. parvum IgA and IgG in seru...
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| Veröffentlicht in: | FEMS microbiology letters 1992-11, Vol.99 (1), p.7-14 |
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| container_title | FEMS microbiology letters |
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| creator | Reperant, Jean‐Michel Naciri, Muriel Chardes, Thierry Bout, Daniel T. |
| description | Cryptosporidium parvum antigens were characterized by immunoblot analysis of sera and intestinal secretions of BALB/c mice orally infected with 105 oocysts. A major band at 17 kDa under non‐reduced conditions and at 18 kDa under reduced conditions was recognized by anti‐C. parvum IgA and IgG in serum and intestinal secretions from day 15 post‐infection. This recognition persisted throughout the experiment (day 30). Mouse‐serum antibodies raised against the 17‐kDa purified antigen (P17) showed no cross‐reactivity with other C. parvum antigens. Immunofluorescence study revealed that this antigen is located on the sporozoite. It is suggested that this antigen could be a good candidate for studies of mucosal immune response to C. parvum and for vaccination. |
| doi_str_mv | 10.1111/j.1574-6968.1992.tb05534.x |
| format | Article |
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A major band at 17 kDa under non‐reduced conditions and at 18 kDa under reduced conditions was recognized by anti‐C. parvum IgA and IgG in serum and intestinal secretions from day 15 post‐infection. This recognition persisted throughout the experiment (day 30). Mouse‐serum antibodies raised against the 17‐kDa purified antigen (P17) showed no cross‐reactivity with other C. parvum antigens. Immunofluorescence study revealed that this antigen is located on the sporozoite. It is suggested that this antigen could be a good candidate for studies of mucosal immune response to C. parvum and for vaccination.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1992.tb05534.x</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Antigenic determinants, haptens, artificial antigens ; Antigens ; Biological and medical sciences ; Cryptosporidium parvum ; Fundamental and applied biological sciences. 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A major band at 17 kDa under non‐reduced conditions and at 18 kDa under reduced conditions was recognized by anti‐C. parvum IgA and IgG in serum and intestinal secretions from day 15 post‐infection. This recognition persisted throughout the experiment (day 30). Mouse‐serum antibodies raised against the 17‐kDa purified antigen (P17) showed no cross‐reactivity with other C. parvum antigens. Immunofluorescence study revealed that this antigen is located on the sporozoite. It is suggested that this antigen could be a good candidate for studies of mucosal immune response to C. parvum and for vaccination.</description><subject>Antigenic determinants, haptens, artificial antigens</subject><subject>Antigens</subject><subject>Biological and medical sciences</subject><subject>Cryptosporidium parvum</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>IgA</subject><subject>Life Sciences</subject><subject>Microbiology and Parasitology</subject><subject>Molecular immunology</subject><subject>Mucosal antigen</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNqVkc9u1DAQhyMEEkvhHSyEkDgkHf9J7CAuq4XSlRZxgbPlOPbWSxIHO2mbcuEReEaepAm72ju-jOT5_M3IvyR5jSHD87k8ZDjnLC3KQmS4LEk2VJDnlGX3T5LVufU0WQHlIsVQ8ufJixgPAMAIFKvk17Ztx843fu-0apC-UUHpwQT3oAbnO-QtUgjzv7___PiokOoGtzcdssG3aBOmfvCx98HVbmxRr8LtXILRft-5B1Mjo0IzoWpC7ah9nPXb_fqfo_K1M_Fl8syqJppXp3qRfL_69G1zne6-ft5u1rtUU0J5WhhbcVwJWtYAdW5ULsDavCgp1iW1XBBgGoqSaCEoZ8AqxoRg2MwU4wzTi-Td0XujGtkH16owSa-cvF7v5HIHhIMQhNwu7Nsj2wf_czRxkK2L2jSN6owfo8QFEzlhdAbfH0EdfIzB2LMZg1yykQe5BCCXAOSSjTxlI-_nx29OU1Scv90G1WkXzwbGCmB4WebDEbtzjZn-Y4C8-rLj9BF1x6RE</recordid><startdate>199211</startdate><enddate>199211</enddate><creator>Reperant, Jean‐Michel</creator><creator>Naciri, Muriel</creator><creator>Chardes, Thierry</creator><creator>Bout, Daniel T.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Wiley-Blackwell</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>M7N</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-2485-1603</orcidid></search><sort><creationdate>199211</creationdate><title>Immunological characterization of a 17‐kDa antigen from Cryptosporidium parvum recognized early by mucosal IgA antibodies</title><author>Reperant, Jean‐Michel ; Naciri, Muriel ; Chardes, Thierry ; Bout, Daniel T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3237-6efb71b839d00d5ea580ff56931c93f78204c0692c8837404b448841e0ff47413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Antigenic determinants, haptens, artificial antigens</topic><topic>Antigens</topic><topic>Biological and medical sciences</topic><topic>Cryptosporidium parvum</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>IgA</topic><topic>Life Sciences</topic><topic>Microbiology and Parasitology</topic><topic>Molecular immunology</topic><topic>Mucosal antigen</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reperant, Jean‐Michel</creatorcontrib><creatorcontrib>Naciri, Muriel</creatorcontrib><creatorcontrib>Chardes, Thierry</creatorcontrib><creatorcontrib>Bout, Daniel T.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reperant, Jean‐Michel</au><au>Naciri, Muriel</au><au>Chardes, Thierry</au><au>Bout, Daniel T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunological characterization of a 17‐kDa antigen from Cryptosporidium parvum recognized early by mucosal IgA antibodies</atitle><jtitle>FEMS microbiology letters</jtitle><date>1992-11</date><risdate>1992</risdate><volume>99</volume><issue>1</issue><spage>7</spage><epage>14</epage><pages>7-14</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Cryptosporidium parvum antigens were characterized by immunoblot analysis of sera and intestinal secretions of BALB/c mice orally infected with 105 oocysts. A major band at 17 kDa under non‐reduced conditions and at 18 kDa under reduced conditions was recognized by anti‐C. parvum IgA and IgG in serum and intestinal secretions from day 15 post‐infection. This recognition persisted throughout the experiment (day 30). Mouse‐serum antibodies raised against the 17‐kDa purified antigen (P17) showed no cross‐reactivity with other C. parvum antigens. Immunofluorescence study revealed that this antigen is located on the sporozoite. It is suggested that this antigen could be a good candidate for studies of mucosal immune response to C. parvum and for vaccination.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1574-6968.1992.tb05534.x</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-2485-1603</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1992-11, Vol.99 (1), p.7-14 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_02708822v1 |
| source | Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals Digital Archive Legacy; Alma/SFX Local Collection |
| subjects | Antigenic determinants, haptens, artificial antigens Antigens Biological and medical sciences Cryptosporidium parvum Fundamental and applied biological sciences. Psychology Fundamental immunology IgA Life Sciences Microbiology and Parasitology Molecular immunology Mucosal antigen |
| title | Immunological characterization of a 17‐kDa antigen from Cryptosporidium parvum recognized early by mucosal IgA antibodies |
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