Photofrin-induced fluorescence in progressive and regressive murine colonic cancer cells: correlation with cell photosensitivity
Microspectrofluorometry and fluorescence imaging were used to investigate the intracellular fluorescence of two murine colonic cancer cell lines — a progressive cell line (PROb) and a regressive cell line (REGb) — incubated with Photofrin. These two cell lines, which were initially cloned from the s...
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| Veröffentlicht in: | Journal of photochemistry and photobiology. B, Biology Biology, 1995-03, Vol.27 (3), p.225-231 |
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| container_title | Journal of photochemistry and photobiology. B, Biology |
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| creator | Blais, J. Amirand, C. Ballini, J.P. Debey, P. Foultier, M.T. Patrice, T. |
| description | Microspectrofluorometry and fluorescence imaging were used to investigate the intracellular fluorescence of two murine colonic cancer cell lines — a progressive cell line (PROb) and a regressive cell line (REGb) — incubated with Photofrin. These two cell lines, which were initially cloned from the same chemically induced colonic murine cancer, differ in their metastic properties and have been considered as models to mimic the tumoral cell heterogeneity.
The fluorescence from cytoplasmic area of cell incubated with Photofrin appeared as a complex emission, with two maxima at 632 and 695 nm assigned to monomer species, and a poorly resolved band around 665 nm assigned to aggregates. The spectral distribution was shown to depend on the incubation time, with an aggregate contribution increasing for extended periods. The amount of Photofrin uptake, as determined from the total fluorescence intensity, was found for PROb to be twice that for REGb. However, the phototoxicities were quite similar for both cell lines, suggesting that drug concentration may not be the only determining factor in photobiological efficiency. |
| doi_str_mv | 10.1016/1011-1344(94)07081-X |
| format | Article |
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The fluorescence from cytoplasmic area of cell incubated with Photofrin appeared as a complex emission, with two maxima at 632 and 695 nm assigned to monomer species, and a poorly resolved band around 665 nm assigned to aggregates. The spectral distribution was shown to depend on the incubation time, with an aggregate contribution increasing for extended periods. The amount of Photofrin uptake, as determined from the total fluorescence intensity, was found for PROb to be twice that for REGb. However, the phototoxicities were quite similar for both cell lines, suggesting that drug concentration may not be the only determining factor in photobiological efficiency.</description><identifier>ISSN: 1011-1344</identifier><identifier>EISSN: 1873-2682</identifier><identifier>EISSN: 1011-1344</identifier><identifier>DOI: 10.1016/1011-1344(94)07081-X</identifier><identifier>PMID: 7769535</identifier><language>eng</language><publisher>Switzerland: Elsevier B.V</publisher><subject>Animals ; Colonic cancer cells ; Colonic Neoplasms - metabolism ; Fluorescence imaging ; Hematoporphyrin Derivative - pharmacokinetics ; Lasers ; Life Sciences ; Metastasis ; Mice ; Microspectrofluorometry ; Microspectrophotometry - instrumentation ; Photochemotherapy ; Photodynamic therapy ; Photofrin ; Time Factors ; Tumor Cells, Cultured</subject><ispartof>Journal of photochemistry and photobiology. B, Biology, 1995-03, Vol.27 (3), p.225-231</ispartof><rights>1995</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-3c7cefe6e06fb660ca7d9c05bbf22078e48116c4c1b7cd170a615c3e1ba665803</citedby><cites>FETCH-LOGICAL-c391t-3c7cefe6e06fb660ca7d9c05bbf22078e48116c4c1b7cd170a615c3e1ba665803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/101113449407081X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7769535$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02708038$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Blais, J.</creatorcontrib><creatorcontrib>Amirand, C.</creatorcontrib><creatorcontrib>Ballini, J.P.</creatorcontrib><creatorcontrib>Debey, P.</creatorcontrib><creatorcontrib>Foultier, M.T.</creatorcontrib><creatorcontrib>Patrice, T.</creatorcontrib><title>Photofrin-induced fluorescence in progressive and regressive murine colonic cancer cells: correlation with cell photosensitivity</title><title>Journal of photochemistry and photobiology. B, Biology</title><addtitle>J Photochem Photobiol B</addtitle><description>Microspectrofluorometry and fluorescence imaging were used to investigate the intracellular fluorescence of two murine colonic cancer cell lines — a progressive cell line (PROb) and a regressive cell line (REGb) — incubated with Photofrin. These two cell lines, which were initially cloned from the same chemically induced colonic murine cancer, differ in their metastic properties and have been considered as models to mimic the tumoral cell heterogeneity.
The fluorescence from cytoplasmic area of cell incubated with Photofrin appeared as a complex emission, with two maxima at 632 and 695 nm assigned to monomer species, and a poorly resolved band around 665 nm assigned to aggregates. The spectral distribution was shown to depend on the incubation time, with an aggregate contribution increasing for extended periods. The amount of Photofrin uptake, as determined from the total fluorescence intensity, was found for PROb to be twice that for REGb. However, the phototoxicities were quite similar for both cell lines, suggesting that drug concentration may not be the only determining factor in photobiological efficiency.</description><subject>Animals</subject><subject>Colonic cancer cells</subject><subject>Colonic Neoplasms - metabolism</subject><subject>Fluorescence imaging</subject><subject>Hematoporphyrin Derivative - pharmacokinetics</subject><subject>Lasers</subject><subject>Life Sciences</subject><subject>Metastasis</subject><subject>Mice</subject><subject>Microspectrofluorometry</subject><subject>Microspectrophotometry - instrumentation</subject><subject>Photochemotherapy</subject><subject>Photodynamic therapy</subject><subject>Photofrin</subject><subject>Time Factors</subject><subject>Tumor Cells, Cultured</subject><issn>1011-1344</issn><issn>1873-2682</issn><issn>1011-1344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU1P3DAQtaoioJR_0Eo-VXBIsfNhJz0gIdQWpJXgUCRuljOZdAdl7a2dLOLWn16H3e4RH8bz8d6MPY-xT1J8lUKqi2RkJouyPGvKc6FFLbPHd-xY1rrIclXn75P_H3LEPsT4JNKplD5kh1qrpiqqY_b3fulH3wdyGbluAux4P0w-YAR0gJwcXwf_O8WRNsit63jAfbiaEhE5-ME7Ag42UQIHHIb4LWVDwMGO5B1_pnH5mufreV5EF2mkDY0vH9lBb4eIp7v7hD38-P7r-iZb3P28vb5aZFA0cswK0IA9KhSqb5USYHXXgKjats9zoWssaykVlCBbDZ3UwipZQYGytUpVtShO2Pm279IOZh1oZcOL8ZbMzdXCzDmRpxWKot7IhP2yxaav_5kwjmZFcX69deinaLTOG61EnYDlFgjBxxiw33eWwswizUaaWQHTlOZVJPOYaJ93_ad2hd2etFMl1S-3dUwL2RAGE4FmOToKCKPpPL094B8rS6QC</recordid><startdate>19950301</startdate><enddate>19950301</enddate><creator>Blais, J.</creator><creator>Amirand, C.</creator><creator>Ballini, J.P.</creator><creator>Debey, P.</creator><creator>Foultier, M.T.</creator><creator>Patrice, T.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>19950301</creationdate><title>Photofrin-induced fluorescence in progressive and regressive murine colonic cancer cells: correlation with cell photosensitivity</title><author>Blais, J. ; Amirand, C. ; Ballini, J.P. ; Debey, P. ; Foultier, M.T. ; Patrice, T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-3c7cefe6e06fb660ca7d9c05bbf22078e48116c4c1b7cd170a615c3e1ba665803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Animals</topic><topic>Colonic cancer cells</topic><topic>Colonic Neoplasms - metabolism</topic><topic>Fluorescence imaging</topic><topic>Hematoporphyrin Derivative - pharmacokinetics</topic><topic>Lasers</topic><topic>Life Sciences</topic><topic>Metastasis</topic><topic>Mice</topic><topic>Microspectrofluorometry</topic><topic>Microspectrophotometry - instrumentation</topic><topic>Photochemotherapy</topic><topic>Photodynamic therapy</topic><topic>Photofrin</topic><topic>Time Factors</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Blais, J.</creatorcontrib><creatorcontrib>Amirand, C.</creatorcontrib><creatorcontrib>Ballini, J.P.</creatorcontrib><creatorcontrib>Debey, P.</creatorcontrib><creatorcontrib>Foultier, M.T.</creatorcontrib><creatorcontrib>Patrice, T.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of photochemistry and photobiology. B, Biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Blais, J.</au><au>Amirand, C.</au><au>Ballini, J.P.</au><au>Debey, P.</au><au>Foultier, M.T.</au><au>Patrice, T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Photofrin-induced fluorescence in progressive and regressive murine colonic cancer cells: correlation with cell photosensitivity</atitle><jtitle>Journal of photochemistry and photobiology. B, Biology</jtitle><addtitle>J Photochem Photobiol B</addtitle><date>1995-03-01</date><risdate>1995</risdate><volume>27</volume><issue>3</issue><spage>225</spage><epage>231</epage><pages>225-231</pages><issn>1011-1344</issn><eissn>1873-2682</eissn><eissn>1011-1344</eissn><abstract>Microspectrofluorometry and fluorescence imaging were used to investigate the intracellular fluorescence of two murine colonic cancer cell lines — a progressive cell line (PROb) and a regressive cell line (REGb) — incubated with Photofrin. These two cell lines, which were initially cloned from the same chemically induced colonic murine cancer, differ in their metastic properties and have been considered as models to mimic the tumoral cell heterogeneity.
The fluorescence from cytoplasmic area of cell incubated with Photofrin appeared as a complex emission, with two maxima at 632 and 695 nm assigned to monomer species, and a poorly resolved band around 665 nm assigned to aggregates. The spectral distribution was shown to depend on the incubation time, with an aggregate contribution increasing for extended periods. The amount of Photofrin uptake, as determined from the total fluorescence intensity, was found for PROb to be twice that for REGb. However, the phototoxicities were quite similar for both cell lines, suggesting that drug concentration may not be the only determining factor in photobiological efficiency.</abstract><cop>Switzerland</cop><pub>Elsevier B.V</pub><pmid>7769535</pmid><doi>10.1016/1011-1344(94)07081-X</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of photochemistry and photobiology. B, Biology, 1995-03, Vol.27 (3), p.225-231 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Colonic cancer cells Colonic Neoplasms - metabolism Fluorescence imaging Hematoporphyrin Derivative - pharmacokinetics Lasers Life Sciences Metastasis Mice Microspectrofluorometry Microspectrophotometry - instrumentation Photochemotherapy Photodynamic therapy Photofrin Time Factors Tumor Cells, Cultured |
| title | Photofrin-induced fluorescence in progressive and regressive murine colonic cancer cells: correlation with cell photosensitivity |
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