Application of gene probes to detect a penaeid shrimp baculovirus in fixed tissue using in situ hybridization

DNA inserts obtained from a cloning of the penaeid shrimp baculovirus, BP, were non-radioactively labelled with digoxigenin for use as gene probes to detect BP in paraffin-embedded, formalin/alcohol-fixed tissue sections using in situ hybridization. Results show that the probes detect BP in various...

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Veröffentlicht in:Diseases of aquatic organisms 1993, Vol.17 (3), p.215-221
Hauptverfasser: Bruce, LD, Redman, RM, Lightner, DV, Bonami, JR
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Sprache:eng
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