Application of gene probes to detect a penaeid shrimp baculovirus in fixed tissue using in situ hybridization
DNA inserts obtained from a cloning of the penaeid shrimp baculovirus, BP, were non-radioactively labelled with digoxigenin for use as gene probes to detect BP in paraffin-embedded, formalin/alcohol-fixed tissue sections using in situ hybridization. Results show that the probes detect BP in various...
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| Veröffentlicht in: | Diseases of aquatic organisms 1993, Vol.17 (3), p.215-221 |
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| container_title | Diseases of aquatic organisms |
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| creator | Bruce, LD Redman, RM Lightner, DV Bonami, JR |
| description | DNA inserts obtained from a cloning of the penaeid shrimp baculovirus, BP, were non-radioactively labelled with digoxigenin for use as gene probes to detect BP in paraffin-embedded, formalin/alcohol-fixed tissue sections using in situ hybridization. Results show that the probes detect BP in various species of shrimp from different geographical areas. The probes demonstrate no reaction to BP-negative tissue sections. The probes also demonstrate no reaction to tissue infected with other penaeid shrimp viruses. |
| doi_str_mv | 10.3354/dao017215 |
| format | Article |
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Results show that the probes detect BP in various species of shrimp from different geographical areas. The probes demonstrate no reaction to BP-negative tissue sections. The probes also demonstrate no reaction to tissue infected with other penaeid shrimp viruses.</description><identifier>ISSN: 0177-5103</identifier><identifier>EISSN: 1616-1580</identifier><identifier>DOI: 10.3354/dao017215</identifier><language>eng</language><publisher>Inter Research</publisher><subject>baculovirus ; Baculovirus penaei ; Life Sciences ; Marine ; Penaeus</subject><ispartof>Diseases of aquatic organisms, 1993, Vol.17 (3), p.215-221</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-46dca0046b2be9a2eb6fcb140ac42765444edfd840f5a40eb76b112ddb11f99c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,3759,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttps://hal.inrae.fr/hal-02706156$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Bruce, LD</creatorcontrib><creatorcontrib>Redman, RM</creatorcontrib><creatorcontrib>Lightner, DV</creatorcontrib><creatorcontrib>Bonami, JR</creatorcontrib><title>Application of gene probes to detect a penaeid shrimp baculovirus in fixed tissue using in situ hybridization</title><title>Diseases of aquatic organisms</title><description>DNA inserts obtained from a cloning of the penaeid shrimp baculovirus, BP, were non-radioactively labelled with digoxigenin for use as gene probes to detect BP in paraffin-embedded, formalin/alcohol-fixed tissue sections using in situ hybridization. Results show that the probes detect BP in various species of shrimp from different geographical areas. The probes demonstrate no reaction to BP-negative tissue sections. The probes also demonstrate no reaction to tissue infected with other penaeid shrimp viruses.</description><subject>baculovirus</subject><subject>Baculovirus penaei</subject><subject>Life Sciences</subject><subject>Marine</subject><subject>Penaeus</subject><issn>0177-5103</issn><issn>1616-1580</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><recordid>eNpFkE9LAzEQxYMoWKsHv0FOgofVJJtku8dS1AoFL3oO-TNpI9vNuskW66d3a0UvM_Dm8ZuZh9A1JXdlKfi905HQilFxgiZUUllQMSOnaDKKVSEoKc_RRUrvhFBWCzpB23nXNcHqHGKLo8draAF3fTSQcI7YQQabscYdtBqCw2nTh22HjbZDE3ehHxIOLfbhExzOIaUB8JBCuz6oKeQBb_amDy58_Wy4RGdeNwmufvsUvT0-vC6Wxerl6XkxXxWWM5YLLp3VhHBpmIFaMzDSW0M50eO8koJzDs67GSdeaE7AVNJQypwbq69rW07R7ZG70Y3qxot1v1dRB7Wcr9RBI6wikgq5o6P35ugdv_4YIGW1DclC0-gW4pAUlbMxq1L8Q20fU-rB_5EpUYfw1V_45Tcaq3ij</recordid><startdate>1993</startdate><enddate>1993</enddate><creator>Bruce, LD</creator><creator>Redman, RM</creator><creator>Lightner, DV</creator><creator>Bonami, JR</creator><general>Inter Research</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7TN</scope><scope>7U9</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H94</scope><scope>H95</scope><scope>H99</scope><scope>L.F</scope><scope>L.G</scope><scope>P64</scope><scope>1XC</scope></search><sort><creationdate>1993</creationdate><title>Application of gene probes to detect a penaeid shrimp baculovirus in fixed tissue using in situ hybridization</title><author>Bruce, LD ; Redman, RM ; Lightner, DV ; Bonami, JR</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-46dca0046b2be9a2eb6fcb140ac42765444edfd840f5a40eb76b112ddb11f99c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>baculovirus</topic><topic>Baculovirus penaei</topic><topic>Life Sciences</topic><topic>Marine</topic><topic>Penaeus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bruce, LD</creatorcontrib><creatorcontrib>Redman, RM</creatorcontrib><creatorcontrib>Lightner, DV</creatorcontrib><creatorcontrib>Bonami, JR</creatorcontrib><collection>CrossRef</collection><collection>Oceanic Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Diseases of aquatic organisms</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bruce, LD</au><au>Redman, RM</au><au>Lightner, DV</au><au>Bonami, JR</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of gene probes to detect a penaeid shrimp baculovirus in fixed tissue using in situ hybridization</atitle><jtitle>Diseases of aquatic organisms</jtitle><date>1993</date><risdate>1993</risdate><volume>17</volume><issue>3</issue><spage>215</spage><epage>221</epage><pages>215-221</pages><issn>0177-5103</issn><eissn>1616-1580</eissn><abstract>DNA inserts obtained from a cloning of the penaeid shrimp baculovirus, BP, were non-radioactively labelled with digoxigenin for use as gene probes to detect BP in paraffin-embedded, formalin/alcohol-fixed tissue sections using in situ hybridization. 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| fulltext | fulltext |
| identifier | ISSN: 0177-5103 |
| ispartof | Diseases of aquatic organisms, 1993, Vol.17 (3), p.215-221 |
| issn | 0177-5103 1616-1580 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_02706156v1 |
| source | Inter-Research; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | baculovirus Baculovirus penaei Life Sciences Marine Penaeus |
| title | Application of gene probes to detect a penaeid shrimp baculovirus in fixed tissue using in situ hybridization |
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