Cryopreservation of cold-acclimated shoot tips of pear in vitro cultures after encapsulation-dehydration
Cryopreservation of axillary shoot-tips of pear in vitro cultures ( Pyrus communis L. cv Beurré Hardy) was performed after encapsulation in alginate beads. Encapsulated shoot-tips were first precultured in medium enriched with sucrose and then dried in a sterile air flow and cooled in liquid nitroge...
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| Veröffentlicht in: | Cryobiology 1992, Vol.29 (6), p.691-700 |
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| creator | Scottez, C. Chevreau, E. Godard, N. Arnaud, Y. Duron, M. Dereuddre, J. |
| description | Cryopreservation of axillary shoot-tips of pear
in vitro cultures (
Pyrus communis L. cv Beurré Hardy) was performed after encapsulation in alginate beads. Encapsulated shoot-tips were first precultured in medium enriched with sucrose and then dried in a sterile air flow and cooled in liquid nitrogen. After slow rewarming in air at room temperature, alginate beads were transferred to solid culture medium for 1 week before removal of shoot-tips from beads and subculture onto fresh medium. Shoot recovery from cryopreserved shoot-tips was greatly improved by 8–12 weeks of cold acclimation at 0 °C of donor
in vitro cultures. The best results (80% shoot recovery) were obtained using 0.75
M sucrose for preculture and 4-h dehydration (giving 20% residual water). The resistance of encapsulated and dehydrated shoot-tips to liquid nitrogen did not depend on cooling rate. Apical shoot-tips about 3 mm in length with several axillary buds were also cryopreserved successfully (47% shoot recovery). |
| doi_str_mv | 10.1016/0011-2240(92)90073-B |
| format | Article |
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in vitro cultures (
Pyrus communis L. cv Beurré Hardy) was performed after encapsulation in alginate beads. Encapsulated shoot-tips were first precultured in medium enriched with sucrose and then dried in a sterile air flow and cooled in liquid nitrogen. After slow rewarming in air at room temperature, alginate beads were transferred to solid culture medium for 1 week before removal of shoot-tips from beads and subculture onto fresh medium. Shoot recovery from cryopreserved shoot-tips was greatly improved by 8–12 weeks of cold acclimation at 0 °C of donor
in vitro cultures. The best results (80% shoot recovery) were obtained using 0.75
M sucrose for preculture and 4-h dehydration (giving 20% residual water). The resistance of encapsulated and dehydrated shoot-tips to liquid nitrogen did not depend on cooling rate. Apical shoot-tips about 3 mm in length with several axillary buds were also cryopreserved successfully (47% shoot recovery).</description><identifier>ISSN: 0011-2240</identifier><identifier>EISSN: 1090-2392</identifier><identifier>DOI: 10.1016/0011-2240(92)90073-B</identifier><identifier>CODEN: CRYBAS</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Agronomy. Soil science and plant productions ; Biological and medical sciences ; COLD ; CONGELACION ; CONGELATION ; CRECIMIENTO ; CROISSANCE ; CULTIVO IN VITRO ; CULTURE IN VITRO ; DEHYDRATION ; DESHIDRATACION ; DESHYDRATATION ; ENCAPSULACION ; ENCAPSULATION ; FREEZING ; FRIO ; FROID ; Fundamental and applied biological sciences. Psychology ; Generalities. Genetics. Plant material ; Genetics and breeding of economic plants ; GERMOPLASMA ; GERMPLASM ; GROWTH ; IN VITRO CULTURE ; Life Sciences ; MATERIEL GENETIQUE ; MERISTEMAS ; MERISTEME ; MERISTEMS ; PHYSIOLOGY ; Plant material ; PRESERVACION ; PRESERVATION ; PYRUS COMMUNIS ; RESISTANCE A LA TEMPERATURE ; RESISTENCIA A LA TEMPERATURA ; SACCHAROSE ; SHOOT MERISTEMS ; STEMS ; SUCROSA ; SUCROSE ; TALLO ; TEMPERATURE RESISTANCE ; TIGE</subject><ispartof>Cryobiology, 1992, Vol.29 (6), p.691-700</ispartof><rights>1992</rights><rights>1993 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c301t-45b8b26d229a85378234b082ab79b85a09641a851c895624f9a09d3843c8fd903</citedby><cites>FETCH-LOGICAL-c301t-45b8b26d229a85378234b082ab79b85a09641a851c895624f9a09d3843c8fd903</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/001122409290073B$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,309,310,314,776,780,785,786,881,3537,4010,4036,4037,23909,23910,25118,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4476542$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02706030$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Scottez, C.</creatorcontrib><creatorcontrib>Chevreau, E.</creatorcontrib><creatorcontrib>Godard, N.</creatorcontrib><creatorcontrib>Arnaud, Y.</creatorcontrib><creatorcontrib>Duron, M.</creatorcontrib><creatorcontrib>Dereuddre, J.</creatorcontrib><title>Cryopreservation of cold-acclimated shoot tips of pear in vitro cultures after encapsulation-dehydration</title><title>Cryobiology</title><description>Cryopreservation of axillary shoot-tips of pear
in vitro cultures (
Pyrus communis L. cv Beurré Hardy) was performed after encapsulation in alginate beads. Encapsulated shoot-tips were first precultured in medium enriched with sucrose and then dried in a sterile air flow and cooled in liquid nitrogen. After slow rewarming in air at room temperature, alginate beads were transferred to solid culture medium for 1 week before removal of shoot-tips from beads and subculture onto fresh medium. Shoot recovery from cryopreserved shoot-tips was greatly improved by 8–12 weeks of cold acclimation at 0 °C of donor
in vitro cultures. The best results (80% shoot recovery) were obtained using 0.75
M sucrose for preculture and 4-h dehydration (giving 20% residual water). The resistance of encapsulated and dehydrated shoot-tips to liquid nitrogen did not depend on cooling rate. Apical shoot-tips about 3 mm in length with several axillary buds were also cryopreserved successfully (47% shoot recovery).</description><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>COLD</subject><subject>CONGELACION</subject><subject>CONGELATION</subject><subject>CRECIMIENTO</subject><subject>CROISSANCE</subject><subject>CULTIVO IN VITRO</subject><subject>CULTURE IN VITRO</subject><subject>DEHYDRATION</subject><subject>DESHIDRATACION</subject><subject>DESHYDRATATION</subject><subject>ENCAPSULACION</subject><subject>ENCAPSULATION</subject><subject>FREEZING</subject><subject>FRIO</subject><subject>FROID</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities. Genetics. Plant material</subject><subject>Genetics and breeding of economic plants</subject><subject>GERMOPLASMA</subject><subject>GERMPLASM</subject><subject>GROWTH</subject><subject>IN VITRO CULTURE</subject><subject>Life Sciences</subject><subject>MATERIEL GENETIQUE</subject><subject>MERISTEMAS</subject><subject>MERISTEME</subject><subject>MERISTEMS</subject><subject>PHYSIOLOGY</subject><subject>Plant material</subject><subject>PRESERVACION</subject><subject>PRESERVATION</subject><subject>PYRUS COMMUNIS</subject><subject>RESISTANCE A LA TEMPERATURE</subject><subject>RESISTENCIA A LA TEMPERATURA</subject><subject>SACCHAROSE</subject><subject>SHOOT MERISTEMS</subject><subject>STEMS</subject><subject>SUCROSA</subject><subject>SUCROSE</subject><subject>TALLO</subject><subject>TEMPERATURE RESISTANCE</subject><subject>TIGE</subject><issn>0011-2240</issn><issn>1090-2392</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNp9kE1rGzEQhkVpoG7SP1B60CGH5rDN6GM_dAkkJm0KhhzanMWspK1VttYiyQb_-2i9wceeJGaed5h5CPnC4BsD1twCMFZxLuGr4jcKoBXVwzuyYqCg4kLx92R1Rj6Qjyn9BYCmFXJFtut4DFN0ycUDZh92NAzUhNFWaMzo_2F2lqZtCJlmP6W5OzmM1O_owecYqNmPeV_yFIfsInU7g1Paj6dZlXXbo42n_xW5GHBM7tPbe0levj_-Xj9Vm-cfP9f3m8oIYLmSdd_1vLGcK-xq0XZcyB46jn2r-q5GUI1kpcNMp-qGy0GVkhWdFKYbrAJxSW6WuVsc9RTLBfGoA3r9dL_Rcw14Cw0IOLDCyoU1MaQU3XAOMNCzWT1r07M2rbg-mdUPJXa9xCZMBsch4s74dM5K2Ta15AX7vGADBo1_YkFefinBFWPzmndL0xUXB--iTsYXec766EzWNvj_L_EK2NCUcg</recordid><startdate>1992</startdate><enddate>1992</enddate><creator>Scottez, C.</creator><creator>Chevreau, E.</creator><creator>Godard, N.</creator><creator>Arnaud, Y.</creator><creator>Duron, M.</creator><creator>Dereuddre, J.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>1XC</scope></search><sort><creationdate>1992</creationdate><title>Cryopreservation of cold-acclimated shoot tips of pear in vitro cultures after encapsulation-dehydration</title><author>Scottez, C. ; Chevreau, E. ; Godard, N. ; Arnaud, Y. ; Duron, M. ; Dereuddre, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c301t-45b8b26d229a85378234b082ab79b85a09641a851c895624f9a09d3843c8fd903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Biological and medical sciences</topic><topic>COLD</topic><topic>CONGELACION</topic><topic>CONGELATION</topic><topic>CRECIMIENTO</topic><topic>CROISSANCE</topic><topic>CULTIVO IN VITRO</topic><topic>CULTURE IN VITRO</topic><topic>DEHYDRATION</topic><topic>DESHIDRATACION</topic><topic>DESHYDRATATION</topic><topic>ENCAPSULACION</topic><topic>ENCAPSULATION</topic><topic>FREEZING</topic><topic>FRIO</topic><topic>FROID</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Generalities. Genetics. Plant material</topic><topic>Genetics and breeding of economic plants</topic><topic>GERMOPLASMA</topic><topic>GERMPLASM</topic><topic>GROWTH</topic><topic>IN VITRO CULTURE</topic><topic>Life Sciences</topic><topic>MATERIEL GENETIQUE</topic><topic>MERISTEMAS</topic><topic>MERISTEME</topic><topic>MERISTEMS</topic><topic>PHYSIOLOGY</topic><topic>Plant material</topic><topic>PRESERVACION</topic><topic>PRESERVATION</topic><topic>PYRUS COMMUNIS</topic><topic>RESISTANCE A LA TEMPERATURE</topic><topic>RESISTENCIA A LA TEMPERATURA</topic><topic>SACCHAROSE</topic><topic>SHOOT MERISTEMS</topic><topic>STEMS</topic><topic>SUCROSA</topic><topic>SUCROSE</topic><topic>TALLO</topic><topic>TEMPERATURE RESISTANCE</topic><topic>TIGE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Scottez, C.</creatorcontrib><creatorcontrib>Chevreau, E.</creatorcontrib><creatorcontrib>Godard, N.</creatorcontrib><creatorcontrib>Arnaud, Y.</creatorcontrib><creatorcontrib>Duron, M.</creatorcontrib><creatorcontrib>Dereuddre, J.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Cryobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Scottez, C.</au><au>Chevreau, E.</au><au>Godard, N.</au><au>Arnaud, Y.</au><au>Duron, M.</au><au>Dereuddre, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cryopreservation of cold-acclimated shoot tips of pear in vitro cultures after encapsulation-dehydration</atitle><jtitle>Cryobiology</jtitle><date>1992</date><risdate>1992</risdate><volume>29</volume><issue>6</issue><spage>691</spage><epage>700</epage><pages>691-700</pages><issn>0011-2240</issn><eissn>1090-2392</eissn><coden>CRYBAS</coden><abstract>Cryopreservation of axillary shoot-tips of pear
in vitro cultures (
Pyrus communis L. cv Beurré Hardy) was performed after encapsulation in alginate beads. Encapsulated shoot-tips were first precultured in medium enriched with sucrose and then dried in a sterile air flow and cooled in liquid nitrogen. After slow rewarming in air at room temperature, alginate beads were transferred to solid culture medium for 1 week before removal of shoot-tips from beads and subculture onto fresh medium. Shoot recovery from cryopreserved shoot-tips was greatly improved by 8–12 weeks of cold acclimation at 0 °C of donor
in vitro cultures. The best results (80% shoot recovery) were obtained using 0.75
M sucrose for preculture and 4-h dehydration (giving 20% residual water). The resistance of encapsulated and dehydrated shoot-tips to liquid nitrogen did not depend on cooling rate. Apical shoot-tips about 3 mm in length with several axillary buds were also cryopreserved successfully (47% shoot recovery).</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><doi>10.1016/0011-2240(92)90073-B</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Cryobiology, 1992, Vol.29 (6), p.691-700 |
| issn | 0011-2240 1090-2392 |
| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | Agronomy. Soil science and plant productions Biological and medical sciences COLD CONGELACION CONGELATION CRECIMIENTO CROISSANCE CULTIVO IN VITRO CULTURE IN VITRO DEHYDRATION DESHIDRATACION DESHYDRATATION ENCAPSULACION ENCAPSULATION FREEZING FRIO FROID Fundamental and applied biological sciences. Psychology Generalities. Genetics. Plant material Genetics and breeding of economic plants GERMOPLASMA GERMPLASM GROWTH IN VITRO CULTURE Life Sciences MATERIEL GENETIQUE MERISTEMAS MERISTEME MERISTEMS PHYSIOLOGY Plant material PRESERVACION PRESERVATION PYRUS COMMUNIS RESISTANCE A LA TEMPERATURE RESISTENCIA A LA TEMPERATURA SACCHAROSE SHOOT MERISTEMS STEMS SUCROSA SUCROSE TALLO TEMPERATURE RESISTANCE TIGE |
| title | Cryopreservation of cold-acclimated shoot tips of pear in vitro cultures after encapsulation-dehydration |
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