Flavor constituents of aqueous fraction extracted from Comte cheese by liquid carbon dioxide

Isolation, purification and identification of some low-molecular-weight compounds from a water-soluble extract of Comte cheese was achieved. We used liquid CO2 extraction, subsequent fractionation (by gel permeation and reserve-phase HPLC) and mass spectrometry. Major constituents were N-acyl amino...

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Veröffentlicht in:Journal of food science 1993-09, Vol.58 (5), p.1005-1009
Hauptverfasser: Roudot-Algaron, F, Le Bars, D, Einhorn, J, Adda, J, Gripon, J.C
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container_end_page 1009
container_issue 5
container_start_page 1005
container_title Journal of food science
container_volume 58
creator Roudot-Algaron, F
Le Bars, D
Einhorn, J
Adda, J
Gripon, J.C
description Isolation, purification and identification of some low-molecular-weight compounds from a water-soluble extract of Comte cheese was achieved. We used liquid CO2 extraction, subsequent fractionation (by gel permeation and reserve-phase HPLC) and mass spectrometry. Major constituents were N-acyl amino acids (N-acetyl methionine, N-propionyl methionine, N-propionyl leucine, N-propionyl phenylalanine), diketopiperazines cyclo(Pro-Pro), cyclo(Pro-Val), cyclo(Pro-Phe), cyclo(Pro-Leu), cyclo(Pro-Ala)) and non-peptide compounds (theobromine, 4-methyl-t-thiazoleethanol, uracil, (5-6)dihydro-uracil, thymine). These compounds were synthesized and tasted. Most were bitter. N-propionyl methionine had a cheese flavor but was not detected when added to a simulated cheese at 180 mg/L
doi_str_mv 10.1111/j.1365-2621.1993.tb06099.x
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We used liquid CO2 extraction, subsequent fractionation (by gel permeation and reserve-phase HPLC) and mass spectrometry. Major constituents were N-acyl amino acids (N-acetyl methionine, N-propionyl methionine, N-propionyl leucine, N-propionyl phenylalanine), diketopiperazines cyclo(Pro-Pro), cyclo(Pro-Val), cyclo(Pro-Phe), cyclo(Pro-Leu), cyclo(Pro-Ala)) and non-peptide compounds (theobromine, 4-methyl-t-thiazoleethanol, uracil, (5-6)dihydro-uracil, thymine). These compounds were synthesized and tasted. Most were bitter. 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We used liquid CO2 extraction, subsequent fractionation (by gel permeation and reserve-phase HPLC) and mass spectrometry. Major constituents were N-acyl amino acids (N-acetyl methionine, N-propionyl methionine, N-propionyl leucine, N-propionyl phenylalanine), diketopiperazines cyclo(Pro-Pro), cyclo(Pro-Val), cyclo(Pro-Phe), cyclo(Pro-Leu), cyclo(Pro-Ala)) and non-peptide compounds (theobromine, 4-methyl-t-thiazoleethanol, uracil, (5-6)dihydro-uracil, thymine). These compounds were synthesized and tasted. Most were bitter. N-propionyl methionine had a cheese flavor but was not detected when added to a simulated cheese at 180 mg/L</description><subject>AROMA</subject><subject>Biological and medical sciences</subject><subject>Carbon dioxide</subject><subject>Cheese</subject><subject>Chemical and Process Engineering</subject><subject>COMPOSICION APROXIMADA</subject><subject>composition</subject><subject>COMPOSITION GLOBALE</subject><subject>comté</subject><subject>DIOXIDO DE CARBONO</subject><subject>DIOXYDE DE CARBONE</subject><subject>Engineering Sciences</subject><subject>FLAVEUR</subject><subject>flavor</subject><subject>Food engineering</subject><subject>Food industries</subject><subject>Food science</subject><subject>FROMAGE</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Life Sciences</subject><subject>liquid CO2</subject><subject>Milk and cheese industries. 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source Wiley Online Library Journals Frontfile Complete
subjects AROMA
Biological and medical sciences
Carbon dioxide
Cheese
Chemical and Process Engineering
COMPOSICION APROXIMADA
composition
COMPOSITION GLOBALE
comté
DIOXIDO DE CARBONO
DIOXYDE DE CARBONE
Engineering Sciences
FLAVEUR
flavor
Food engineering
Food industries
Food science
FROMAGE
Fundamental and applied biological sciences. Psychology
Life Sciences
liquid CO2
Milk and cheese industries. Ice creams
QUESO
Taste
title Flavor constituents of aqueous fraction extracted from Comte cheese by liquid carbon dioxide
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