Molecular cloning of GRA4, a Toxoplasma gondii dense granule protein, recognized by mucosal IgA antibodies
Clones which were selected from a Toxoplasma gondii expression library with the immune serum from a T. gondii-infected rabbit, were further screened using milk and intestinal secretions from mice which had been orally infected with T. gondii cysts. The gene products of several clones reacted strongl...
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| Veröffentlicht in: | Molecular and biochemical parasitology 1992-12, Vol.56 (2), p.227-238 |
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| container_title | Molecular and biochemical parasitology |
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| creator | Mevelec, Marie-Noëlle Chardès, Thierry Mercereau-Puijalon, Odile Bourguin, Isabelle Achbarou, Abderahim Dubremetz, Jean-François Bout, Daniel |
| description | Clones which were selected from a
Toxoplasma gondii expression library with the immune serum from a
T. gondii-infected rabbit, were further screened using milk and intestinal secretions from mice which had been orally infected with
T. gondii cysts. The gene products of several clones reacted strongly with milk IgA and weakly with intestinal IgA. Three of these clones (5.1, 36.1, 37.4) were shown to encode a dense granule protein of 40 kDa (GRA4). The GRA4 protein co-migrates with one of the
T. gondii antigens recognized by mucosal IgA. The complete nucleotide sequence of GRA4 has been obtained by cloning genomic
T. gondii BamHI fragments containing the 37.4 DNA insert. The coding sequence contains no intron. The deduced amino acid sequence indicates a proline rich (12%) product with an internal hydrophobic region of 19 amino acids and a potential site of
N-glycosylation. The primary translation product with a theoretical size of 36 260 Da contains a putative N-terminal signal sequence of 20 amino acids but no apparent glycolipid anchor sequence. Quantitation of the
GRA4 gene and Southern blot analysis suggested that the
GRA4 gene is single copy.
GRA4 gene is translated in tachyzoites to yield a single mRNA species of about 1900 bases. |
| doi_str_mv | 10.1016/0166-6851(92)90172-G |
| format | Article |
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Toxoplasma gondii expression library with the immune serum from a
T. gondii-infected rabbit, were further screened using milk and intestinal secretions from mice which had been orally infected with
T. gondii cysts. The gene products of several clones reacted strongly with milk IgA and weakly with intestinal IgA. Three of these clones (5.1, 36.1, 37.4) were shown to encode a dense granule protein of 40 kDa (GRA4). The GRA4 protein co-migrates with one of the
T. gondii antigens recognized by mucosal IgA. The complete nucleotide sequence of GRA4 has been obtained by cloning genomic
T. gondii BamHI fragments containing the 37.4 DNA insert. The coding sequence contains no intron. The deduced amino acid sequence indicates a proline rich (12%) product with an internal hydrophobic region of 19 amino acids and a potential site of
N-glycosylation. The primary translation product with a theoretical size of 36 260 Da contains a putative N-terminal signal sequence of 20 amino acids but no apparent glycolipid anchor sequence. Quantitation of the
GRA4 gene and Southern blot analysis suggested that the
GRA4 gene is single copy.
GRA4 gene is translated in tachyzoites to yield a single mRNA species of about 1900 bases.</description><identifier>ISSN: 0166-6851</identifier><identifier>EISSN: 1872-9428</identifier><identifier>DOI: 10.1016/0166-6851(92)90172-G</identifier><identifier>PMID: 1362450</identifier><identifier>CODEN: MBIPDP</identifier><language>eng</language><publisher>Shannon: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies, Protozoan - biosynthesis ; Antibodies, Protozoan - immunology ; Base Sequence ; Biological and medical sciences ; Cloning, Molecular ; Cytoplasmic Granules - chemistry ; Dense granule ; DNA ; Female ; Fundamental and applied biological sciences. Psychology ; Genes, Protozoan - genetics ; Genes. Genome ; IgA ; Immunoglobulin A - immunology ; Intestinal Mucosa - immunology ; Invertebrates ; Life Sciences ; Mice ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Poly A - genetics ; Polymerase Chain Reaction ; Pregnancy ; Pregnancy Complications, Parasitic - immunology ; Proline-rich protein ; Protozoan Proteins - genetics ; Protozoan Proteins - immunology ; RNA, Messenger - genetics ; Sequence Analysis, DNA ; Toxoplasma - genetics ; Toxoplasma - immunology ; Toxoplasma gondii ; Toxoplasmosis, Animal - immunology</subject><ispartof>Molecular and biochemical parasitology, 1992-12, Vol.56 (2), p.227-238</ispartof><rights>1992</rights><rights>1993 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c517t-d3db5174387ce6236bd512a446374809e13dcdfcd1799ede4e29672c7de3e6903</citedby><cites>FETCH-LOGICAL-c517t-d3db5174387ce6236bd512a446374809e13dcdfcd1799ede4e29672c7de3e6903</cites><orcidid>0000-0001-6784-8549</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/016668519290172G$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4557249$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1362450$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02705390$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Mevelec, Marie-Noëlle</creatorcontrib><creatorcontrib>Chardès, Thierry</creatorcontrib><creatorcontrib>Mercereau-Puijalon, Odile</creatorcontrib><creatorcontrib>Bourguin, Isabelle</creatorcontrib><creatorcontrib>Achbarou, Abderahim</creatorcontrib><creatorcontrib>Dubremetz, Jean-François</creatorcontrib><creatorcontrib>Bout, Daniel</creatorcontrib><title>Molecular cloning of GRA4, a Toxoplasma gondii dense granule protein, recognized by mucosal IgA antibodies</title><title>Molecular and biochemical parasitology</title><addtitle>Mol Biochem Parasitol</addtitle><description>Clones which were selected from a
Toxoplasma gondii expression library with the immune serum from a
T. gondii-infected rabbit, were further screened using milk and intestinal secretions from mice which had been orally infected with
T. gondii cysts. The gene products of several clones reacted strongly with milk IgA and weakly with intestinal IgA. Three of these clones (5.1, 36.1, 37.4) were shown to encode a dense granule protein of 40 kDa (GRA4). The GRA4 protein co-migrates with one of the
T. gondii antigens recognized by mucosal IgA. The complete nucleotide sequence of GRA4 has been obtained by cloning genomic
T. gondii BamHI fragments containing the 37.4 DNA insert. The coding sequence contains no intron. The deduced amino acid sequence indicates a proline rich (12%) product with an internal hydrophobic region of 19 amino acids and a potential site of
N-glycosylation. The primary translation product with a theoretical size of 36 260 Da contains a putative N-terminal signal sequence of 20 amino acids but no apparent glycolipid anchor sequence. Quantitation of the
GRA4 gene and Southern blot analysis suggested that the
GRA4 gene is single copy.
GRA4 gene is translated in tachyzoites to yield a single mRNA species of about 1900 bases.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Protozoan - biosynthesis</subject><subject>Antibodies, Protozoan - immunology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Cytoplasmic Granules - chemistry</subject><subject>Dense granule</subject><subject>DNA</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Protozoan - genetics</subject><subject>Genes. Genome</subject><subject>IgA</subject><subject>Immunoglobulin A - immunology</subject><subject>Intestinal Mucosa - immunology</subject><subject>Invertebrates</subject><subject>Life Sciences</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Poly A - genetics</subject><subject>Polymerase Chain Reaction</subject><subject>Pregnancy</subject><subject>Pregnancy Complications, Parasitic - immunology</subject><subject>Proline-rich protein</subject><subject>Protozoan Proteins - genetics</subject><subject>Protozoan Proteins - immunology</subject><subject>RNA, Messenger - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Toxoplasma - genetics</subject><subject>Toxoplasma - immunology</subject><subject>Toxoplasma gondii</subject><subject>Toxoplasmosis, Animal - immunology</subject><issn>0166-6851</issn><issn>1872-9428</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkd-L1DAQgIso53r6HyjkQcSDq-Z3mhdhOXTvYEWQ8zmkybTmyDZr0x6ef71Zu6xv-hAyzHwzTPJV1UuC3xFM5PtyZC0bQd5qeqExUbTePKpWpCmB5rR5XK1OyNPqWc53GGOhpDyrzgiTlAu8qu4-pwhujnZELqYhDD1KHdp8XfNLZNFt-pn20eadRX0afAjIw5AB9aMd5ghoP6YJwnCJRnCpH8Iv8Kh9QLvZpWwjuunXyA5TaJMPkJ9XTzobM7w43ufVt08fb6-u6-2Xzc3Vels7QdRUe-bbEnDWKAeSMtl6QajlXDLFG6yBMO985zxRWoMHDlRLRZ3ywEBqzM6ri2XudxvNfgw7Oz6YZIO5Xm_NIYepwoJpfE8K-2Zhy0t-zJAnswvZQYx2gDRnoxgXkjXivyCRQjYM0wLyBXRjynmE7rQCwebgzRykmIMUo6n5481sStur4_y53YH_27SIKvXXx7rNzsauCHAhnzAuhKJcF-zDgkH54PsAo8kuwODAh6JoMj6Ff-_xG9gnsl4</recordid><startdate>19921201</startdate><enddate>19921201</enddate><creator>Mevelec, Marie-Noëlle</creator><creator>Chardès, Thierry</creator><creator>Mercereau-Puijalon, Odile</creator><creator>Bourguin, Isabelle</creator><creator>Achbarou, Abderahim</creator><creator>Dubremetz, Jean-François</creator><creator>Bout, Daniel</creator><general>Elsevier B.V</general><general>Elsevier Science</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-6784-8549</orcidid></search><sort><creationdate>19921201</creationdate><title>Molecular cloning of GRA4, a Toxoplasma gondii dense granule protein, recognized by mucosal IgA antibodies</title><author>Mevelec, Marie-Noëlle ; Chardès, Thierry ; Mercereau-Puijalon, Odile ; Bourguin, Isabelle ; Achbarou, Abderahim ; Dubremetz, Jean-François ; Bout, Daniel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c517t-d3db5174387ce6236bd512a446374809e13dcdfcd1799ede4e29672c7de3e6903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Protozoan - biosynthesis</topic><topic>Antibodies, Protozoan - immunology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>Cytoplasmic Granules - chemistry</topic><topic>Dense granule</topic><topic>DNA</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Protozoan - genetics</topic><topic>Genes. Genome</topic><topic>IgA</topic><topic>Immunoglobulin A - immunology</topic><topic>Intestinal Mucosa - immunology</topic><topic>Invertebrates</topic><topic>Life Sciences</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Poly A - genetics</topic><topic>Polymerase Chain Reaction</topic><topic>Pregnancy</topic><topic>Pregnancy Complications, Parasitic - immunology</topic><topic>Proline-rich protein</topic><topic>Protozoan Proteins - genetics</topic><topic>Protozoan Proteins - immunology</topic><topic>RNA, Messenger - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Toxoplasma - genetics</topic><topic>Toxoplasma - immunology</topic><topic>Toxoplasma gondii</topic><topic>Toxoplasmosis, Animal - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mevelec, Marie-Noëlle</creatorcontrib><creatorcontrib>Chardès, Thierry</creatorcontrib><creatorcontrib>Mercereau-Puijalon, Odile</creatorcontrib><creatorcontrib>Bourguin, Isabelle</creatorcontrib><creatorcontrib>Achbarou, Abderahim</creatorcontrib><creatorcontrib>Dubremetz, Jean-François</creatorcontrib><creatorcontrib>Bout, Daniel</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Molecular and biochemical parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mevelec, Marie-Noëlle</au><au>Chardès, Thierry</au><au>Mercereau-Puijalon, Odile</au><au>Bourguin, Isabelle</au><au>Achbarou, Abderahim</au><au>Dubremetz, Jean-François</au><au>Bout, Daniel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning of GRA4, a Toxoplasma gondii dense granule protein, recognized by mucosal IgA antibodies</atitle><jtitle>Molecular and biochemical parasitology</jtitle><addtitle>Mol Biochem Parasitol</addtitle><date>1992-12-01</date><risdate>1992</risdate><volume>56</volume><issue>2</issue><spage>227</spage><epage>238</epage><pages>227-238</pages><issn>0166-6851</issn><eissn>1872-9428</eissn><coden>MBIPDP</coden><abstract>Clones which were selected from a
Toxoplasma gondii expression library with the immune serum from a
T. gondii-infected rabbit, were further screened using milk and intestinal secretions from mice which had been orally infected with
T. gondii cysts. The gene products of several clones reacted strongly with milk IgA and weakly with intestinal IgA. Three of these clones (5.1, 36.1, 37.4) were shown to encode a dense granule protein of 40 kDa (GRA4). The GRA4 protein co-migrates with one of the
T. gondii antigens recognized by mucosal IgA. The complete nucleotide sequence of GRA4 has been obtained by cloning genomic
T. gondii BamHI fragments containing the 37.4 DNA insert. The coding sequence contains no intron. The deduced amino acid sequence indicates a proline rich (12%) product with an internal hydrophobic region of 19 amino acids and a potential site of
N-glycosylation. The primary translation product with a theoretical size of 36 260 Da contains a putative N-terminal signal sequence of 20 amino acids but no apparent glycolipid anchor sequence. Quantitation of the
GRA4 gene and Southern blot analysis suggested that the
GRA4 gene is single copy.
GRA4 gene is translated in tachyzoites to yield a single mRNA species of about 1900 bases.</abstract><cop>Shannon</cop><pub>Elsevier B.V</pub><pmid>1362450</pmid><doi>10.1016/0166-6851(92)90172-G</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-6784-8549</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0166-6851 |
| ispartof | Molecular and biochemical parasitology, 1992-12, Vol.56 (2), p.227-238 |
| issn | 0166-6851 1872-9428 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_02705390v1 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Animals Antibodies, Protozoan - biosynthesis Antibodies, Protozoan - immunology Base Sequence Biological and medical sciences Cloning, Molecular Cytoplasmic Granules - chemistry Dense granule DNA Female Fundamental and applied biological sciences. Psychology Genes, Protozoan - genetics Genes. Genome IgA Immunoglobulin A - immunology Intestinal Mucosa - immunology Invertebrates Life Sciences Mice Molecular and cellular biology Molecular genetics Molecular Sequence Data Poly A - genetics Polymerase Chain Reaction Pregnancy Pregnancy Complications, Parasitic - immunology Proline-rich protein Protozoan Proteins - genetics Protozoan Proteins - immunology RNA, Messenger - genetics Sequence Analysis, DNA Toxoplasma - genetics Toxoplasma - immunology Toxoplasma gondii Toxoplasmosis, Animal - immunology |
| title | Molecular cloning of GRA4, a Toxoplasma gondii dense granule protein, recognized by mucosal IgA antibodies |
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