Expression of the tobacco Tnt1 retrotransposon promoter in heterologous species
The expression of the tobacco (Nicotiana tabacum) retrotransposon Tnt1 has previously been shown to be strongly regulated and driven from the 5' long terminal repeat (LTR). We report here that the Tnt1 LTR can promote activity of the beta-glucuronidase (GUS) reporter gene in two heterologous sp...
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| Veröffentlicht in: | Plant molecular biology 1994-10, Vol.26 (1), p.393-402 |
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| creator | Pauls, P.K. (Laboratoire de Biologie Cellulaire INRA, Versailles (France)) Kunert, K Huttner, E Grandbastien, M.A |
| description | The expression of the tobacco (Nicotiana tabacum) retrotransposon Tnt1 has previously been shown to be strongly regulated and driven from the 5' long terminal repeat (LTR). We report here that the Tnt1 LTR can promote activity of the beta-glucuronidase (GUS) reporter gene in two heterologous species of the Brassicaceae family, namely rapeseed (Brassica napus) and Arabidopsis thaliana. The translational LTR-GUS fusion was active in transient expression studies performed with tobacco and rapeseed protoplasts, indicating that the LTR sequences are recognized in heterologous species. Our results also showed that Tnt1 LTR-promoted GUS expression in transgenic Arabidopsis is strongly regulated, and that, in contrast to tobacco, hormonal activation plays a significant role in the expression of the Tnt1 LTR in Arabidopsis. LTR sequences were shown to be more effective than the CaMV 35S enhancer region in transient expression studies performed with tobacco or rapeseed protoplasts, and substitution of the LTR sequences upstream from the major transcriptional start with the CaMV 35S enhancer region gave high levels of expression in transgenic tobacco and Arabidopsis leaves, suggesting that a Tnt1 element with similar substitutions in its 5' LTR might be suited for gene-tagging experiments in heterologous species. |
| doi_str_mv | 10.1007/BF00039548 |
| format | Article |
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(Laboratoire de Biologie Cellulaire INRA, Versailles (France)) ; Kunert, K ; Huttner, E ; Grandbastien, M.A</creator><creatorcontrib>Pauls, P.K. (Laboratoire de Biologie Cellulaire INRA, Versailles (France)) ; Kunert, K ; Huttner, E ; Grandbastien, M.A</creatorcontrib><description>The expression of the tobacco (Nicotiana tabacum) retrotransposon Tnt1 has previously been shown to be strongly regulated and driven from the 5' long terminal repeat (LTR). We report here that the Tnt1 LTR can promote activity of the beta-glucuronidase (GUS) reporter gene in two heterologous species of the Brassicaceae family, namely rapeseed (Brassica napus) and Arabidopsis thaliana. The translational LTR-GUS fusion was active in transient expression studies performed with tobacco and rapeseed protoplasts, indicating that the LTR sequences are recognized in heterologous species. Our results also showed that Tnt1 LTR-promoted GUS expression in transgenic Arabidopsis is strongly regulated, and that, in contrast to tobacco, hormonal activation plays a significant role in the expression of the Tnt1 LTR in Arabidopsis. LTR sequences were shown to be more effective than the CaMV 35S enhancer region in transient expression studies performed with tobacco or rapeseed protoplasts, and substitution of the LTR sequences upstream from the major transcriptional start with the CaMV 35S enhancer region gave high levels of expression in transgenic tobacco and Arabidopsis leaves, suggesting that a Tnt1 element with similar substitutions in its 5' LTR might be suited for gene-tagging experiments in heterologous species.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/BF00039548</identifier><identifier>PMID: 7948885</identifier><language>eng</language><publisher>Netherlands: Springer Verlag (Germany)</publisher><subject>Arabidopsis - genetics ; ARABIDOPSIS THALIANA ; Biochemistry, Molecular Biology ; Brassica - genetics ; BRASSICA NAPUS ; cauliflower mosaic virus ; EXPRESION GENICA ; EXPRESSION DES GENES ; GENE ; GENE EXPRESSION ; Gene Expression Regulation, Plant - genetics ; GENE TRANSPOSITION ; GENES ; Genes, Reporter ; Glucuronidase - genetics ; Life Sciences ; Nicotiana - genetics ; NICOTIANA TABACUM ; PLANTAS TRANSGENICAS ; PLANTE TRANSGENIQUE ; Plants, Genetically Modified ; Plants, Toxic ; Promoter Regions, Genetic - genetics ; Protoplasts ; Recombinant Fusion Proteins - biosynthesis ; Repetitive Sequences, Nucleic Acid - genetics ; Retroelements - genetics ; Species Specificity ; TRANSGENIC PLANTS ; TRANSPOSICION DE GENES ; TRANSPOSITION DE GENES</subject><ispartof>Plant molecular biology, 1994-10, Vol.26 (1), p.393-402</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c366t-7eb5f8e32a1d60ef293ef8e4ff9f3ba79ae1bddeaedb46261753bcef25c7a20e3</citedby><cites>FETCH-LOGICAL-c366t-7eb5f8e32a1d60ef293ef8e4ff9f3ba79ae1bddeaedb46261753bcef25c7a20e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7948885$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02704867$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Pauls, P.K. (Laboratoire de Biologie Cellulaire INRA, Versailles (France))</creatorcontrib><creatorcontrib>Kunert, K</creatorcontrib><creatorcontrib>Huttner, E</creatorcontrib><creatorcontrib>Grandbastien, M.A</creatorcontrib><title>Expression of the tobacco Tnt1 retrotransposon promoter in heterologous species</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>The expression of the tobacco (Nicotiana tabacum) retrotransposon Tnt1 has previously been shown to be strongly regulated and driven from the 5' long terminal repeat (LTR). We report here that the Tnt1 LTR can promote activity of the beta-glucuronidase (GUS) reporter gene in two heterologous species of the Brassicaceae family, namely rapeseed (Brassica napus) and Arabidopsis thaliana. The translational LTR-GUS fusion was active in transient expression studies performed with tobacco and rapeseed protoplasts, indicating that the LTR sequences are recognized in heterologous species. Our results also showed that Tnt1 LTR-promoted GUS expression in transgenic Arabidopsis is strongly regulated, and that, in contrast to tobacco, hormonal activation plays a significant role in the expression of the Tnt1 LTR in Arabidopsis. LTR sequences were shown to be more effective than the CaMV 35S enhancer region in transient expression studies performed with tobacco or rapeseed protoplasts, and substitution of the LTR sequences upstream from the major transcriptional start with the CaMV 35S enhancer region gave high levels of expression in transgenic tobacco and Arabidopsis leaves, suggesting that a Tnt1 element with similar substitutions in its 5' LTR might be suited for gene-tagging experiments in heterologous species.</description><subject>Arabidopsis - genetics</subject><subject>ARABIDOPSIS THALIANA</subject><subject>Biochemistry, Molecular Biology</subject><subject>Brassica - genetics</subject><subject>BRASSICA NAPUS</subject><subject>cauliflower mosaic virus</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>GENE</subject><subject>GENE EXPRESSION</subject><subject>Gene Expression Regulation, Plant - genetics</subject><subject>GENE TRANSPOSITION</subject><subject>GENES</subject><subject>Genes, Reporter</subject><subject>Glucuronidase - genetics</subject><subject>Life Sciences</subject><subject>Nicotiana - genetics</subject><subject>NICOTIANA TABACUM</subject><subject>PLANTAS TRANSGENICAS</subject><subject>PLANTE TRANSGENIQUE</subject><subject>Plants, Genetically Modified</subject><subject>Plants, Toxic</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Protoplasts</subject><subject>Recombinant Fusion Proteins - biosynthesis</subject><subject>Repetitive Sequences, Nucleic Acid - genetics</subject><subject>Retroelements - genetics</subject><subject>Species Specificity</subject><subject>TRANSGENIC PLANTS</subject><subject>TRANSPOSICION DE GENES</subject><subject>TRANSPOSITION DE GENES</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkc1LwzAYxoMoc04vHgWhJ0GhmjRN0h7n2JxQ3GWeQ5q-2SpdU5NO9L83o0NP79ePh4fnReia4EeCsXh6XmCMac7S7ASNCRM0ZjjJTtEYEy7iNCXJObrw_gPjgFM-QiORp1mWsTFazb87B97Xto2sifotRL0tldY2Wrc9iRz0zvZOtb6zPjCdszvbg4vqNtpCaGxjN3bvI9-BrsFfojOjGg9XxzpB74v5eraMi9XL62xaxJpy3scCSmYyoIkiFcdgkpxCmFNjckNLJXIFpKwqUFCVKU84EYyWOnBMC5VgoBN0P-huVSM7V--U-5FW1XI5LeRhhxOB04yLLxLYu4EN5j_34Hu5q72GplEtBOuScM5xSg_gwwBqZ713YP6UCZaHpOV_0gG-Paruyx1Uf-gx2nC_Ge5GWak2rvbyrchZeAnN6S8O0YHM</recordid><startdate>19941001</startdate><enddate>19941001</enddate><creator>Pauls, P.K. 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(Laboratoire de Biologie Cellulaire INRA, Versailles (France))</creatorcontrib><creatorcontrib>Kunert, K</creatorcontrib><creatorcontrib>Huttner, E</creatorcontrib><creatorcontrib>Grandbastien, M.A</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pauls, P.K. (Laboratoire de Biologie Cellulaire INRA, Versailles (France))</au><au>Kunert, K</au><au>Huttner, E</au><au>Grandbastien, M.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of the tobacco Tnt1 retrotransposon promoter in heterologous species</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1994-10-01</date><risdate>1994</risdate><volume>26</volume><issue>1</issue><spage>393</spage><epage>402</epage><pages>393-402</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><abstract>The expression of the tobacco (Nicotiana tabacum) retrotransposon Tnt1 has previously been shown to be strongly regulated and driven from the 5' long terminal repeat (LTR). We report here that the Tnt1 LTR can promote activity of the beta-glucuronidase (GUS) reporter gene in two heterologous species of the Brassicaceae family, namely rapeseed (Brassica napus) and Arabidopsis thaliana. The translational LTR-GUS fusion was active in transient expression studies performed with tobacco and rapeseed protoplasts, indicating that the LTR sequences are recognized in heterologous species. Our results also showed that Tnt1 LTR-promoted GUS expression in transgenic Arabidopsis is strongly regulated, and that, in contrast to tobacco, hormonal activation plays a significant role in the expression of the Tnt1 LTR in Arabidopsis. LTR sequences were shown to be more effective than the CaMV 35S enhancer region in transient expression studies performed with tobacco or rapeseed protoplasts, and substitution of the LTR sequences upstream from the major transcriptional start with the CaMV 35S enhancer region gave high levels of expression in transgenic tobacco and Arabidopsis leaves, suggesting that a Tnt1 element with similar substitutions in its 5' LTR might be suited for gene-tagging experiments in heterologous species.</abstract><cop>Netherlands</cop><pub>Springer Verlag (Germany)</pub><pmid>7948885</pmid><doi>10.1007/BF00039548</doi><tpages>10</tpages></addata></record> |
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| identifier | ISSN: 0167-4412 |
| ispartof | Plant molecular biology, 1994-10, Vol.26 (1), p.393-402 |
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| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Arabidopsis - genetics ARABIDOPSIS THALIANA Biochemistry, Molecular Biology Brassica - genetics BRASSICA NAPUS cauliflower mosaic virus EXPRESION GENICA EXPRESSION DES GENES GENE GENE EXPRESSION Gene Expression Regulation, Plant - genetics GENE TRANSPOSITION GENES Genes, Reporter Glucuronidase - genetics Life Sciences Nicotiana - genetics NICOTIANA TABACUM PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE Plants, Genetically Modified Plants, Toxic Promoter Regions, Genetic - genetics Protoplasts Recombinant Fusion Proteins - biosynthesis Repetitive Sequences, Nucleic Acid - genetics Retroelements - genetics Species Specificity TRANSGENIC PLANTS TRANSPOSICION DE GENES TRANSPOSITION DE GENES |
| title | Expression of the tobacco Tnt1 retrotransposon promoter in heterologous species |
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