Stathmin gene expression in mammary gland and in Nb2 cells
Summary— Mammary gland growth occurs essentially during pregnancy and induction of milk synthesis is triggered at parturition. Prolactin is mammogenic in vivo but only marginally in vitro. Prolactin induces milk synthesis in vivo and in cultured mammary cells. Prolactin is also strictly required for...
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| Veröffentlicht in: | Biology of the cell 1995-01, Vol.85 (2-3), p.109-115 |
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| creator | Puissant, Claudine Mitev, Vanio Lemnaouar, Mustapha Manceau, Valérie Sobel, André Houdebine, Louis-Marie |
| description | Summary— Mammary gland growth occurs essentially during pregnancy and induction of milk synthesis is triggered at parturition. Prolactin is mammogenic in vivo but only marginally in vitro. Prolactin induces milk synthesis in vivo and in cultured mammary cells. Prolactin is also strictly required for the multiplication of the rat lymphoid Nb2 cells. Stathmin is an ubiquitous and highly conserved phosphoprotein which seems to be involved in the intracellular mechanisms which trigger cell multiplication and differentiation. In the present study, the concentration of stathmin mRNA has been evaluated during the pregnancy‐lactation‐weaning cycle in mouse and rabbit. Stathmin mRNA appeared at its highest level during pregnancy and it was almost undetectable during lactation. Prolactin injected into mid‐pregnant rabbits induced milk synthesis and this effect was not accompanied by any modification of stathmin mRNA concentration. In cultured primary rabbit mammary cells, prolactin induced casein gene expression without any alteration of stathmin mRNA concentration. In Nb2 cells, prolactin induced a progressive increase of stathmin mRNA concentration. This effect was not significant until after 4 h of prolactin action. These data suggest that stathmin is involved in mammary and Nb2 cell multiplication but may not be necessary for mammary cell differentiation. |
| doi_str_mv | 10.1016/0248-4900(96)85271-3 |
| format | Article |
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Prolactin is mammogenic in vivo but only marginally in vitro. Prolactin induces milk synthesis in vivo and in cultured mammary cells. Prolactin is also strictly required for the multiplication of the rat lymphoid Nb2 cells. Stathmin is an ubiquitous and highly conserved phosphoprotein which seems to be involved in the intracellular mechanisms which trigger cell multiplication and differentiation. In the present study, the concentration of stathmin mRNA has been evaluated during the pregnancy‐lactation‐weaning cycle in mouse and rabbit. Stathmin mRNA appeared at its highest level during pregnancy and it was almost undetectable during lactation. Prolactin injected into mid‐pregnant rabbits induced milk synthesis and this effect was not accompanied by any modification of stathmin mRNA concentration. In cultured primary rabbit mammary cells, prolactin induced casein gene expression without any alteration of stathmin mRNA concentration. In Nb2 cells, prolactin induced a progressive increase of stathmin mRNA concentration. This effect was not significant until after 4 h of prolactin action. These data suggest that stathmin is involved in mammary and Nb2 cell multiplication but may not be necessary for mammary cell differentiation.</description><identifier>ISSN: 0248-4900</identifier><identifier>EISSN: 1768-322X</identifier><identifier>DOI: 10.1016/0248-4900(96)85271-3</identifier><identifier>PMID: 8785512</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Actins - biosynthesis ; Actins - genetics ; Animals ; Caseins - biosynthesis ; Caseins - genetics ; Female ; Gene Expression Regulation ; Gene Expression Regulation, Neoplastic ; Lactation - genetics ; Life Sciences ; Lymphoma, T-Cell - pathology ; mammary cell ; Mammary Glands, Animal - drug effects ; Mammary Glands, Animal - metabolism ; Mice ; Microtubule Proteins ; Nb2 cell ; Neoplasm Proteins - biosynthesis ; Neoplasm Proteins - genetics ; Neoplasms, Hormone-Dependent - pathology ; Phosphoproteins - biosynthesis ; Phosphoproteins - genetics ; Pregnancy ; Pregnancy, Animal - genetics ; prolactin ; Prolactin - pharmacology ; Rabbits ; Rats ; RNA, Messenger - analysis ; RNA, Neoplasm - analysis ; Stathmin ; Tumor Cells, Cultured - drug effects</subject><ispartof>Biology of the cell, 1995-01, Vol.85 (2-3), p.109-115</ispartof><rights>1995 Société Française des Microscopies and Société Biologie Cellulaire de France</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8785512$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02704456$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Puissant, Claudine</creatorcontrib><creatorcontrib>Mitev, Vanio</creatorcontrib><creatorcontrib>Lemnaouar, Mustapha</creatorcontrib><creatorcontrib>Manceau, Valérie</creatorcontrib><creatorcontrib>Sobel, André</creatorcontrib><creatorcontrib>Houdebine, Louis-Marie</creatorcontrib><title>Stathmin gene expression in mammary gland and in Nb2 cells</title><title>Biology of the cell</title><addtitle>Biol Cell</addtitle><description>Summary— Mammary gland growth occurs essentially during pregnancy and induction of milk synthesis is triggered at parturition. Prolactin is mammogenic in vivo but only marginally in vitro. Prolactin induces milk synthesis in vivo and in cultured mammary cells. Prolactin is also strictly required for the multiplication of the rat lymphoid Nb2 cells. Stathmin is an ubiquitous and highly conserved phosphoprotein which seems to be involved in the intracellular mechanisms which trigger cell multiplication and differentiation. In the present study, the concentration of stathmin mRNA has been evaluated during the pregnancy‐lactation‐weaning cycle in mouse and rabbit. Stathmin mRNA appeared at its highest level during pregnancy and it was almost undetectable during lactation. Prolactin injected into mid‐pregnant rabbits induced milk synthesis and this effect was not accompanied by any modification of stathmin mRNA concentration. In cultured primary rabbit mammary cells, prolactin induced casein gene expression without any alteration of stathmin mRNA concentration. In Nb2 cells, prolactin induced a progressive increase of stathmin mRNA concentration. This effect was not significant until after 4 h of prolactin action. These data suggest that stathmin is involved in mammary and Nb2 cell multiplication but may not be necessary for mammary cell differentiation.</description><subject>Actins - biosynthesis</subject><subject>Actins - genetics</subject><subject>Animals</subject><subject>Caseins - biosynthesis</subject><subject>Caseins - genetics</subject><subject>Female</subject><subject>Gene Expression Regulation</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Lactation - genetics</subject><subject>Life Sciences</subject><subject>Lymphoma, T-Cell - pathology</subject><subject>mammary cell</subject><subject>Mammary Glands, Animal - drug effects</subject><subject>Mammary Glands, Animal - metabolism</subject><subject>Mice</subject><subject>Microtubule Proteins</subject><subject>Nb2 cell</subject><subject>Neoplasm Proteins - biosynthesis</subject><subject>Neoplasm Proteins - genetics</subject><subject>Neoplasms, Hormone-Dependent - pathology</subject><subject>Phosphoproteins - biosynthesis</subject><subject>Phosphoproteins - genetics</subject><subject>Pregnancy</subject><subject>Pregnancy, Animal - genetics</subject><subject>prolactin</subject><subject>Prolactin - pharmacology</subject><subject>Rabbits</subject><subject>Rats</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Neoplasm - analysis</subject><subject>Stathmin</subject><subject>Tumor Cells, Cultured - drug effects</subject><issn>0248-4900</issn><issn>1768-322X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9UNlKw0AUHUTRuvyBQp5EH6J3JrNkfLPFtmJRcEHfLhN7x0aTtGZal783saUPlwtn43AYO-RwxoHrcxAyjaUFOLH6NFXC8DjZYB1udBonQrxsss5assN2Q3gHAGlTtc22U5MqxUWHXTzM3XxS5lX0RhVF9DOrKYR8WkUNVLqydPVv9Fa4ahy114C3mYheqSjCPtvyrgh0sPp77Kl_9dgbxqO7wXXvchRPhExsbMQrp0xlRipSyhsuwJD3ZmzBZNZ5T3LsudQ-kVIrDyRSsFxS01CT1TzZY6fL3IkrcFbnbSWcuhyHlyNsMRAGpFT6q9UeL7Wzevq5oDDHMg9tW1fRdBHQNKlgQTXCo5VwkZU0Xueuhml4u-S_84J-1zQHbLfHdlhsh0Wr8X97TLB71xOc28YbL715mNPP2uvqD9QmMQqfbwfYvR8Y89y_wW7yBwbdgqE</recordid><startdate>199501</startdate><enddate>199501</enddate><creator>Puissant, Claudine</creator><creator>Mitev, Vanio</creator><creator>Lemnaouar, Mustapha</creator><creator>Manceau, Valérie</creator><creator>Sobel, André</creator><creator>Houdebine, Louis-Marie</creator><general>Blackwell Publishing Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>199501</creationdate><title>Stathmin gene expression in mammary gland and in Nb2 cells</title><author>Puissant, Claudine ; Mitev, Vanio ; Lemnaouar, Mustapha ; Manceau, Valérie ; Sobel, André ; Houdebine, Louis-Marie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h2439-72c1eb5b745e55f71207eff7d907b9affe4df146f34465f0e280914e7856e9613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Actins - biosynthesis</topic><topic>Actins - genetics</topic><topic>Animals</topic><topic>Caseins - biosynthesis</topic><topic>Caseins - genetics</topic><topic>Female</topic><topic>Gene Expression Regulation</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Lactation - genetics</topic><topic>Life Sciences</topic><topic>Lymphoma, T-Cell - pathology</topic><topic>mammary cell</topic><topic>Mammary Glands, Animal - drug effects</topic><topic>Mammary Glands, Animal - metabolism</topic><topic>Mice</topic><topic>Microtubule Proteins</topic><topic>Nb2 cell</topic><topic>Neoplasm Proteins - biosynthesis</topic><topic>Neoplasm Proteins - genetics</topic><topic>Neoplasms, Hormone-Dependent - pathology</topic><topic>Phosphoproteins - biosynthesis</topic><topic>Phosphoproteins - genetics</topic><topic>Pregnancy</topic><topic>Pregnancy, Animal - genetics</topic><topic>prolactin</topic><topic>Prolactin - pharmacology</topic><topic>Rabbits</topic><topic>Rats</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Neoplasm - analysis</topic><topic>Stathmin</topic><topic>Tumor Cells, Cultured - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Puissant, Claudine</creatorcontrib><creatorcontrib>Mitev, Vanio</creatorcontrib><creatorcontrib>Lemnaouar, Mustapha</creatorcontrib><creatorcontrib>Manceau, Valérie</creatorcontrib><creatorcontrib>Sobel, André</creatorcontrib><creatorcontrib>Houdebine, Louis-Marie</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Biology of the cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Puissant, Claudine</au><au>Mitev, Vanio</au><au>Lemnaouar, Mustapha</au><au>Manceau, Valérie</au><au>Sobel, André</au><au>Houdebine, Louis-Marie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stathmin gene expression in mammary gland and in Nb2 cells</atitle><jtitle>Biology of the cell</jtitle><addtitle>Biol Cell</addtitle><date>1995-01</date><risdate>1995</risdate><volume>85</volume><issue>2-3</issue><spage>109</spage><epage>115</epage><pages>109-115</pages><issn>0248-4900</issn><eissn>1768-322X</eissn><abstract>Summary— Mammary gland growth occurs essentially during pregnancy and induction of milk synthesis is triggered at parturition. Prolactin is mammogenic in vivo but only marginally in vitro. Prolactin induces milk synthesis in vivo and in cultured mammary cells. Prolactin is also strictly required for the multiplication of the rat lymphoid Nb2 cells. Stathmin is an ubiquitous and highly conserved phosphoprotein which seems to be involved in the intracellular mechanisms which trigger cell multiplication and differentiation. In the present study, the concentration of stathmin mRNA has been evaluated during the pregnancy‐lactation‐weaning cycle in mouse and rabbit. Stathmin mRNA appeared at its highest level during pregnancy and it was almost undetectable during lactation. Prolactin injected into mid‐pregnant rabbits induced milk synthesis and this effect was not accompanied by any modification of stathmin mRNA concentration. In cultured primary rabbit mammary cells, prolactin induced casein gene expression without any alteration of stathmin mRNA concentration. In Nb2 cells, prolactin induced a progressive increase of stathmin mRNA concentration. This effect was not significant until after 4 h of prolactin action. These data suggest that stathmin is involved in mammary and Nb2 cell multiplication but may not be necessary for mammary cell differentiation.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>8785512</pmid><doi>10.1016/0248-4900(96)85271-3</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biology of the cell, 1995-01, Vol.85 (2-3), p.109-115 |
| issn | 0248-4900 1768-322X |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Actins - biosynthesis Actins - genetics Animals Caseins - biosynthesis Caseins - genetics Female Gene Expression Regulation Gene Expression Regulation, Neoplastic Lactation - genetics Life Sciences Lymphoma, T-Cell - pathology mammary cell Mammary Glands, Animal - drug effects Mammary Glands, Animal - metabolism Mice Microtubule Proteins Nb2 cell Neoplasm Proteins - biosynthesis Neoplasm Proteins - genetics Neoplasms, Hormone-Dependent - pathology Phosphoproteins - biosynthesis Phosphoproteins - genetics Pregnancy Pregnancy, Animal - genetics prolactin Prolactin - pharmacology Rabbits Rats RNA, Messenger - analysis RNA, Neoplasm - analysis Stathmin Tumor Cells, Cultured - drug effects |
| title | Stathmin gene expression in mammary gland and in Nb2 cells |
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