Construction of a chimeric viral gene expressing plum pox virus coat protein

The capsid-encoding gene of plum pox virus (PPV) was fused with the leader sequence of the coat protein mRNA ( cp) of tobacco mosaic virus by a novel mutagenesis technique which involves reverse transcription of minus-strand RNA [synthesized by in vitro transcription of a double-stranded (ds) cDNA c...

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Veröffentlicht in:	Gene 1992-10, Vol.120 (2), p.167-173
Hauptverfasser:	Ravelonandro, Michel, Monsion, Marie, Teycheney, Pierre Yves, Delbos, René, Dunez, Jean
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN ARN MENSAJERO ARN MESSAGER Base Sequence Biological and medical sciences capsid Capsid - genetics Chimera Cloning, Molecular DNA Fundamental and applied biological sciences. Psychology GENE GENES Genes, Viral Genetics Life Sciences MESSENGER RNA Molecular and cellular biology Molecular genetics Molecular Sequence Data MUTACION Mutagenesis MUTATION NICOTIANA Nicotiana plants Plant Viruses - genetics PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE Plants, Genetically Modified PLASMIDE PLASMIDIOS PLASMIDS PLUM POX VIRUS Potyvirus Protein Sorting Signals - genetics recombinant plasmids Restriction Mapping RNA, Messenger - genetics RNA, Messenger - metabolism tobacco mosaic virus Tobacco Mosaic Virus - genetics Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing transgene TRANSGENIC PLANTS VIRUS SHARKA DEL CIRUELO VIRUS SHARKA DU PRUNIER
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