Cloning and expression in yeast of a plant potassium ion transport system

A membrane polypeptide involved in K+ transport in a higher plant was cloned by complementation of a yeast mutant defective in K+ uptake with a complementary DNA library from Arabidopsis thaliana. A 2.65-kilobase complementary DNA conferred ability to grow on media with K+ concentration in the micro...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 1992-05, Vol.256 (5057), p.663-665
Hauptverfasser:	Sentenac, H. (Biochimie et Physiologie Vegetales, Montpellier, France), Bonneaud, N, Minet, M, Lacroute, F, Salmon, J.M, Gaymard, F, Grignon, C
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino acids Ankyrins Arabidopsis Proteins Arabidopsis thaliana Biological and medical sciences Biological Transport Blotting, Southern Carrier Proteins - chemistry Carrier Proteins - genetics cDNA CDNA libraries Cell physiology Cellular biology CLONACION CLONAGE Cloning Cloning, Molecular Complementary DNA CRUCIFERAE Cytology Deoxyribonuclease EcoRI Deoxyribonucleic acid DNA DNA - genetics EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology Gene Expression genes Kinetics Life Sciences Literary Devices Membrane and intracellular transports Molecular and cellular biology Molecular Sequence Data NUCLEOTIDE nucleotide sequence NUCLEOTIDOS Plant cells Plant Proteins - chemistry Plant Proteins - genetics Plants - genetics Plasmids POTASIO POTASSIUM Potassium - metabolism Potassium channels Potassium Channels - chemistry Potassium Channels - genetics predictions SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - genetics Sequence Homology, Nucleic Acid String theory systems Topography Transcripts (Written Records) transport Yeast Yeast (Food product) Yeasts
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