Chicken Granulosa Cell Proliferation and Progesterone Production in Culture: Effects of EGF and Theca Secretions
Paracrine/autocrine factors like epidermal growth factor (EGF) and transforming growth factor (TGFα) produced in the mammalian ovary modulate ovarian functions such as cell proliferation, differentiation, and steroidogenesis. To study similar activities in the avian ovary, we have developed a cultur...
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| Veröffentlicht in: | General and comparative endocrinology 1994-06, Vol.94 (3), p.341-356 |
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| creator | Peddle, M.J. Onagbesan, O.M. Williams, J. |
| description | Paracrine/autocrine factors like epidermal growth factor (EGF) and transforming growth factor (TGFα) produced in the mammalian ovary modulate ovarian functions such as cell proliferation, differentiation, and steroidogenesis. To study similar activities in the avian ovary, we have developed a culture system for chicken granulosa cells using either serum containing or serum-free media. In the defined medium, granulosa cells for the F1, F2, and F3 follicles of the chicken ovary proliferated as indicated by enhanced levels of DNA, synthesised progesterone in similar amounts to those observed in acute experiments, and responded to luteinizing hormone (LH) during and after the culture period. The morphology of the cells and profile of growth in both media (although better in serum than in serum-free medium) are similar to those reported for mammalian cells. EGF stimulated granulosa cell proliferation over 72 hr in culture while inhibiting basal and LH-stimulated progesterone production; these effects were greatest in the less mature cells taken from the F3 follicles. Theca-conditioned medium (TCM) from chicken ovarian theca cell cultures mimicked the effects of EGF when cultured with granulosa cells. Taken together, these results suggest that EGF/GFα-like peptides have a role in avian ovarian function. Moreover, the effects observed for TCM suggest that these peptides may be present in the theca layer with a paracrine function in the adjacent granulosa. |
| doi_str_mv | 10.1006/gcen.1994.1090 |
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To study similar activities in the avian ovary, we have developed a culture system for chicken granulosa cells using either serum containing or serum-free media. In the defined medium, granulosa cells for the F1, F2, and F3 follicles of the chicken ovary proliferated as indicated by enhanced levels of DNA, synthesised progesterone in similar amounts to those observed in acute experiments, and responded to luteinizing hormone (LH) during and after the culture period. The morphology of the cells and profile of growth in both media (although better in serum than in serum-free medium) are similar to those reported for mammalian cells. EGF stimulated granulosa cell proliferation over 72 hr in culture while inhibiting basal and LH-stimulated progesterone production; these effects were greatest in the less mature cells taken from the F3 follicles. Theca-conditioned medium (TCM) from chicken ovarian theca cell cultures mimicked the effects of EGF when cultured with granulosa cells. Taken together, these results suggest that EGF/GFα-like peptides have a role in avian ovarian function. Moreover, the effects observed for TCM suggest that these peptides may be present in the theca layer with a paracrine function in the adjacent granulosa.</description><identifier>ISSN: 0016-6480</identifier><identifier>EISSN: 1095-6840</identifier><identifier>DOI: 10.1006/gcen.1994.1090</identifier><identifier>PMID: 7926642</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>ADN ; Animals ; BIOSINTESIS ; BIOSYNTHESE ; Cell Division - physiology ; Cells, Cultured ; CELLULE ; CELULAS ; Chickens ; CRECIMIENTO ; CROISSANCE ; CULTIVO DE CELULAS ; CULTURE DE CELLULE ; Culture Media, Conditioned ; Culture Media, Serum-Free ; DNA - biosynthesis ; Endocrinology and metabolism ; Epidermal Growth Factor - physiology ; Female ; FEMELLE ; Granulosa Cells - physiology ; Granulosa Cells - ultrastructure ; HEMBRA ; HORMONAS ; HORMONE ; Human health and pathology ; Humans ; Life Sciences ; Luteinizing Hormone - pharmacology ; MEDIO DE CULTIVO ; MILIEU DE CULTURE ; Ovary - cytology ; Ovary - metabolism ; PEPTIDE ; PEPTIDOS ; POLLO ; POULET ; PROGESTERONA ; PROGESTERONE ; Progesterone - biosynthesis ; PROMOTEUR DE CROISSANCE ANIMALE ; PROMOTORES DEL CRECIMIENTO ANIMAL ; Recombinant Proteins - pharmacology ; SECRECION ; SECRETION ; Theca Cells - physiology ; Theca Cells - ultrastructure ; Tumor Necrosis Factor-alpha - pharmacology</subject><ispartof>General and comparative endocrinology, 1994-06, Vol.94 (3), p.341-356</ispartof><rights>1994 Academic Press</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c487t-bcfbbf8c3d91b3bdd3476ffb45d34a90a6fdf6b1c230f027d274fe21ad0d57813</citedby><orcidid>0000-0002-4688-3000</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0016648084710902$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7926642$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02701615$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Peddle, M.J.</creatorcontrib><creatorcontrib>Onagbesan, O.M.</creatorcontrib><creatorcontrib>Williams, J.</creatorcontrib><title>Chicken Granulosa Cell Proliferation and Progesterone Production in Culture: Effects of EGF and Theca Secretions</title><title>General and comparative endocrinology</title><addtitle>Gen Comp Endocrinol</addtitle><description>Paracrine/autocrine factors like epidermal growth factor (EGF) and transforming growth factor (TGFα) produced in the mammalian ovary modulate ovarian functions such as cell proliferation, differentiation, and steroidogenesis. To study similar activities in the avian ovary, we have developed a culture system for chicken granulosa cells using either serum containing or serum-free media. In the defined medium, granulosa cells for the F1, F2, and F3 follicles of the chicken ovary proliferated as indicated by enhanced levels of DNA, synthesised progesterone in similar amounts to those observed in acute experiments, and responded to luteinizing hormone (LH) during and after the culture period. The morphology of the cells and profile of growth in both media (although better in serum than in serum-free medium) are similar to those reported for mammalian cells. EGF stimulated granulosa cell proliferation over 72 hr in culture while inhibiting basal and LH-stimulated progesterone production; these effects were greatest in the less mature cells taken from the F3 follicles. Theca-conditioned medium (TCM) from chicken ovarian theca cell cultures mimicked the effects of EGF when cultured with granulosa cells. Taken together, these results suggest that EGF/GFα-like peptides have a role in avian ovarian function. Moreover, the effects observed for TCM suggest that these peptides may be present in the theca layer with a paracrine function in the adjacent granulosa.</description><subject>ADN</subject><subject>Animals</subject><subject>BIOSINTESIS</subject><subject>BIOSYNTHESE</subject><subject>Cell Division - physiology</subject><subject>Cells, Cultured</subject><subject>CELLULE</subject><subject>CELULAS</subject><subject>Chickens</subject><subject>CRECIMIENTO</subject><subject>CROISSANCE</subject><subject>CULTIVO DE CELULAS</subject><subject>CULTURE DE CELLULE</subject><subject>Culture Media, Conditioned</subject><subject>Culture Media, Serum-Free</subject><subject>DNA - biosynthesis</subject><subject>Endocrinology and metabolism</subject><subject>Epidermal Growth Factor - physiology</subject><subject>Female</subject><subject>FEMELLE</subject><subject>Granulosa Cells - physiology</subject><subject>Granulosa Cells - ultrastructure</subject><subject>HEMBRA</subject><subject>HORMONAS</subject><subject>HORMONE</subject><subject>Human health and pathology</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Luteinizing Hormone - pharmacology</subject><subject>MEDIO DE CULTIVO</subject><subject>MILIEU DE CULTURE</subject><subject>Ovary - cytology</subject><subject>Ovary - metabolism</subject><subject>PEPTIDE</subject><subject>PEPTIDOS</subject><subject>POLLO</subject><subject>POULET</subject><subject>PROGESTERONA</subject><subject>PROGESTERONE</subject><subject>Progesterone - biosynthesis</subject><subject>PROMOTEUR DE CROISSANCE ANIMALE</subject><subject>PROMOTORES DEL CRECIMIENTO ANIMAL</subject><subject>Recombinant Proteins - pharmacology</subject><subject>SECRECION</subject><subject>SECRETION</subject><subject>Theca Cells - physiology</subject><subject>Theca Cells - ultrastructure</subject><subject>Tumor Necrosis Factor-alpha - pharmacology</subject><issn>0016-6480</issn><issn>1095-6840</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1UcFq3DAQFaUh3aa99lAo-BTowVvJliW7t2A2m8JCCknOQpZGu2q90layA_37SvGSW0-jmffmDXoPoU8ErwnG7NtegVuTrqOp7fAbtEqlKVlL8Vu0wpiwktEWv0PvY_yFMW5qRi7RJe8qxmi1Qqf-YNVvcMU2SDePPsqih3EsfgY_WgNBTta7QjqdJ3uIEwTvIDd6Vi-YdUU_j9Mc4HuxMQbUFAtvis329mXt8QBKFg-gAmR6_IAujBwjfDzXK_R0u3ns78rd_fZHf7MrFW35VA7KDINpVa07MtSD1jXlzJiBNuklOyyZ0YYNRFU1NrjiuuLUQEWkxrrhLamv0NdF9yBHcQr2KMNf4aUVdzc7kWdpKblDmufMvV64p-D_zOmT4mijSjZIB36OgjNOOW94Iq4Xogo-xgDmVZlgkeMQOQ6R4xA5jrTw5aw8D0fQr_Sz_wn_vOBGeiH3wUbx9NA1uGEVS2C7gJB8erYQRFQWnAJtQ7JZaG__d_cfJH6iAg</recordid><startdate>19940601</startdate><enddate>19940601</enddate><creator>Peddle, M.J.</creator><creator>Onagbesan, O.M.</creator><creator>Williams, J.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-4688-3000</orcidid></search><sort><creationdate>19940601</creationdate><title>Chicken Granulosa Cell Proliferation and Progesterone Production in Culture: Effects of EGF and Theca Secretions</title><author>Peddle, M.J. ; Onagbesan, O.M. ; Williams, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-bcfbbf8c3d91b3bdd3476ffb45d34a90a6fdf6b1c230f027d274fe21ad0d57813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>ADN</topic><topic>Animals</topic><topic>BIOSINTESIS</topic><topic>BIOSYNTHESE</topic><topic>Cell Division - physiology</topic><topic>Cells, Cultured</topic><topic>CELLULE</topic><topic>CELULAS</topic><topic>Chickens</topic><topic>CRECIMIENTO</topic><topic>CROISSANCE</topic><topic>CULTIVO DE CELULAS</topic><topic>CULTURE DE CELLULE</topic><topic>Culture Media, Conditioned</topic><topic>Culture Media, Serum-Free</topic><topic>DNA - biosynthesis</topic><topic>Endocrinology and metabolism</topic><topic>Epidermal Growth Factor - physiology</topic><topic>Female</topic><topic>FEMELLE</topic><topic>Granulosa Cells - physiology</topic><topic>Granulosa Cells - ultrastructure</topic><topic>HEMBRA</topic><topic>HORMONAS</topic><topic>HORMONE</topic><topic>Human health and pathology</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>Luteinizing Hormone - pharmacology</topic><topic>MEDIO DE CULTIVO</topic><topic>MILIEU DE CULTURE</topic><topic>Ovary - cytology</topic><topic>Ovary - metabolism</topic><topic>PEPTIDE</topic><topic>PEPTIDOS</topic><topic>POLLO</topic><topic>POULET</topic><topic>PROGESTERONA</topic><topic>PROGESTERONE</topic><topic>Progesterone - biosynthesis</topic><topic>PROMOTEUR DE CROISSANCE ANIMALE</topic><topic>PROMOTORES DEL CRECIMIENTO ANIMAL</topic><topic>Recombinant Proteins - pharmacology</topic><topic>SECRECION</topic><topic>SECRETION</topic><topic>Theca Cells - physiology</topic><topic>Theca Cells - ultrastructure</topic><topic>Tumor Necrosis Factor-alpha - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peddle, M.J.</creatorcontrib><creatorcontrib>Onagbesan, O.M.</creatorcontrib><creatorcontrib>Williams, J.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>General and comparative endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peddle, M.J.</au><au>Onagbesan, O.M.</au><au>Williams, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chicken Granulosa Cell Proliferation and Progesterone Production in Culture: Effects of EGF and Theca Secretions</atitle><jtitle>General and comparative endocrinology</jtitle><addtitle>Gen Comp Endocrinol</addtitle><date>1994-06-01</date><risdate>1994</risdate><volume>94</volume><issue>3</issue><spage>341</spage><epage>356</epage><pages>341-356</pages><issn>0016-6480</issn><eissn>1095-6840</eissn><abstract>Paracrine/autocrine factors like epidermal growth factor (EGF) and transforming growth factor (TGFα) produced in the mammalian ovary modulate ovarian functions such as cell proliferation, differentiation, and steroidogenesis. To study similar activities in the avian ovary, we have developed a culture system for chicken granulosa cells using either serum containing or serum-free media. In the defined medium, granulosa cells for the F1, F2, and F3 follicles of the chicken ovary proliferated as indicated by enhanced levels of DNA, synthesised progesterone in similar amounts to those observed in acute experiments, and responded to luteinizing hormone (LH) during and after the culture period. The morphology of the cells and profile of growth in both media (although better in serum than in serum-free medium) are similar to those reported for mammalian cells. EGF stimulated granulosa cell proliferation over 72 hr in culture while inhibiting basal and LH-stimulated progesterone production; these effects were greatest in the less mature cells taken from the F3 follicles. Theca-conditioned medium (TCM) from chicken ovarian theca cell cultures mimicked the effects of EGF when cultured with granulosa cells. Taken together, these results suggest that EGF/GFα-like peptides have a role in avian ovarian function. Moreover, the effects observed for TCM suggest that these peptides may be present in the theca layer with a paracrine function in the adjacent granulosa.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7926642</pmid><doi>10.1006/gcen.1994.1090</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0002-4688-3000</orcidid></addata></record> |
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| ispartof | General and comparative endocrinology, 1994-06, Vol.94 (3), p.341-356 |
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| subjects | ADN Animals BIOSINTESIS BIOSYNTHESE Cell Division - physiology Cells, Cultured CELLULE CELULAS Chickens CRECIMIENTO CROISSANCE CULTIVO DE CELULAS CULTURE DE CELLULE Culture Media, Conditioned Culture Media, Serum-Free DNA - biosynthesis Endocrinology and metabolism Epidermal Growth Factor - physiology Female FEMELLE Granulosa Cells - physiology Granulosa Cells - ultrastructure HEMBRA HORMONAS HORMONE Human health and pathology Humans Life Sciences Luteinizing Hormone - pharmacology MEDIO DE CULTIVO MILIEU DE CULTURE Ovary - cytology Ovary - metabolism PEPTIDE PEPTIDOS POLLO POULET PROGESTERONA PROGESTERONE Progesterone - biosynthesis PROMOTEUR DE CROISSANCE ANIMALE PROMOTORES DEL CRECIMIENTO ANIMAL Recombinant Proteins - pharmacology SECRECION SECRETION Theca Cells - physiology Theca Cells - ultrastructure Tumor Necrosis Factor-alpha - pharmacology |
| title | Chicken Granulosa Cell Proliferation and Progesterone Production in Culture: Effects of EGF and Theca Secretions |
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