Dietary Phytic Acid and Wheat Bran Enhance Mucosal Phytase Activity in Rat Small Intestine
The aim of this work was to investigate the influence of dietary phytic acid (PA) on intestinal phytase activity in growing rats by in vitro determination of phytase activity in the three segments of the small intestine (duodenum, jejunum and ileum), and by in vivo intestinal perfusion of a solution...
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| Veröffentlicht in: | The Journal of nutrition 2000-08, Vol.130 (8), p.2020-2025 |
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| creator | Lopez, Hubert W. Vallery, Franck Levrat-Verny, Marie-Anne Coudray, Charles Demigné, Christian Rémésy, Christian |
| description | The aim of this work was to investigate the influence of dietary phytic acid (PA) on intestinal phytase activity in growing rats by in vitro determination of phytase activity in the three segments of the small intestine (duodenum, jejunum and ileum), and by in vivo intestinal perfusion of a solution rich in PA (diluted soymilk). Using the in vitro method, duodenal and jejunal activities were enhanced significantly by adaptation to purified PA (+44 and +145% respectively, compared with control rats). For the rats adapted to the wheat bran (WB) diet, the induction of intestinal phytase by the substrate compared with the control values (P < 0.001) was observed only in ileum. Using soymilk in perfusions, rats consuming PA or WB diets hydrolyzed more phytate (P < 0.001 and P < 0.05, respectively) than controls. Further, Mg absorption from diluted soymilk was not affected by food adaptation, whereas Ca absorption was greater in the PA and WB groups (P < 0.001 and P < 0.05, respectively) than in the control group. Thus, intake of pure PA by rats enhances phytase in the upper parts of the small intestine (duodenum and jejunum), whereas the WB diet activates ileal phytase. Furthermore, the induction of phytase activity is greater in magnitude in rats fed synthetic PA than that observed in rats fed the WB diet. The enhancement of phytase improves intestinal Ca absorption, thus showing the capacity of the small intestine to adapt to diets rich in PA and poor in Ca. |
| doi_str_mv | 10.1093/jn/130.8.2020 |
| format | Article |
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Using the in vitro method, duodenal and jejunal activities were enhanced significantly by adaptation to purified PA (+44 and +145% respectively, compared with control rats). For the rats adapted to the wheat bran (WB) diet, the induction of intestinal phytase by the substrate compared with the control values (P < 0.001) was observed only in ileum. Using soymilk in perfusions, rats consuming PA or WB diets hydrolyzed more phytate (P < 0.001 and P < 0.05, respectively) than controls. Further, Mg absorption from diluted soymilk was not affected by food adaptation, whereas Ca absorption was greater in the PA and WB groups (P < 0.001 and P < 0.05, respectively) than in the control group. Thus, intake of pure PA by rats enhances phytase in the upper parts of the small intestine (duodenum and jejunum), whereas the WB diet activates ileal phytase. Furthermore, the induction of phytase activity is greater in magnitude in rats fed synthetic PA than that observed in rats fed the WB diet. The enhancement of phytase improves intestinal Ca absorption, thus showing the capacity of the small intestine to adapt to diets rich in PA and poor in Ca.</description><identifier>ISSN: 0022-3166</identifier><identifier>EISSN: 1541-6100</identifier><identifier>DOI: 10.1093/jn/130.8.2020</identifier><identifier>PMID: 10917919</identifier><identifier>CODEN: JONUAI</identifier><language>eng</language><publisher>Bethesda, MD: Elsevier Inc</publisher><subject>6-Phytase - biosynthesis ; absorption ; Acids ; Animals ; Biological and medical sciences ; Calcium ; degradation ; Dietary Fiber - pharmacology ; Enzyme Induction ; Food and Nutrition ; Fundamental and applied biological sciences. Psychology ; Glycine max ; Intestinal Mucosa - drug effects ; Intestinal Mucosa - enzymology ; Intestine, Small - drug effects ; Intestine, Small - enzymology ; Intestine. Mesentery ; Life Sciences ; magnesium ; Male ; Milk ; Nutrition ; phytate ; Phytic Acid - pharmacology ; Rats ; Rats, Wistar ; Rodents ; Small intestine ; Vertebrates: digestive system</subject><ispartof>The Journal of nutrition, 2000-08, Vol.130 (8), p.2020-2025</ispartof><rights>2000 American Society for Nutrition.</rights><rights>2000 INIST-CNRS</rights><rights>Copyright American Institute of Nutrition Aug 2000</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c532t-ddb5f7a66cd8748b97db60d7566c5505f6087d40c29665a92d6fe422274344fe3</citedby><cites>FETCH-LOGICAL-c532t-ddb5f7a66cd8748b97db60d7566c5505f6087d40c29665a92d6fe422274344fe3</cites><orcidid>0000-0001-6295-9389 ; 0000-0003-2680-7796</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,777,781,882,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1535919$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10917919$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02697740$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Lopez, Hubert W.</creatorcontrib><creatorcontrib>Vallery, Franck</creatorcontrib><creatorcontrib>Levrat-Verny, Marie-Anne</creatorcontrib><creatorcontrib>Coudray, Charles</creatorcontrib><creatorcontrib>Demigné, Christian</creatorcontrib><creatorcontrib>Rémésy, Christian</creatorcontrib><title>Dietary Phytic Acid and Wheat Bran Enhance Mucosal Phytase Activity in Rat Small Intestine</title><title>The Journal of nutrition</title><addtitle>J Nutr</addtitle><description>The aim of this work was to investigate the influence of dietary phytic acid (PA) on intestinal phytase activity in growing rats by in vitro determination of phytase activity in the three segments of the small intestine (duodenum, jejunum and ileum), and by in vivo intestinal perfusion of a solution rich in PA (diluted soymilk). Using the in vitro method, duodenal and jejunal activities were enhanced significantly by adaptation to purified PA (+44 and +145% respectively, compared with control rats). For the rats adapted to the wheat bran (WB) diet, the induction of intestinal phytase by the substrate compared with the control values (P < 0.001) was observed only in ileum. Using soymilk in perfusions, rats consuming PA or WB diets hydrolyzed more phytate (P < 0.001 and P < 0.05, respectively) than controls. Further, Mg absorption from diluted soymilk was not affected by food adaptation, whereas Ca absorption was greater in the PA and WB groups (P < 0.001 and P < 0.05, respectively) than in the control group. Thus, intake of pure PA by rats enhances phytase in the upper parts of the small intestine (duodenum and jejunum), whereas the WB diet activates ileal phytase. Furthermore, the induction of phytase activity is greater in magnitude in rats fed synthetic PA than that observed in rats fed the WB diet. The enhancement of phytase improves intestinal Ca absorption, thus showing the capacity of the small intestine to adapt to diets rich in PA and poor in Ca.</description><subject>6-Phytase - biosynthesis</subject><subject>absorption</subject><subject>Acids</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcium</subject><subject>degradation</subject><subject>Dietary Fiber - pharmacology</subject><subject>Enzyme Induction</subject><subject>Food and Nutrition</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycine max</subject><subject>Intestinal Mucosa - drug effects</subject><subject>Intestinal Mucosa - enzymology</subject><subject>Intestine, Small - drug effects</subject><subject>Intestine, Small - enzymology</subject><subject>Intestine. Mesentery</subject><subject>Life Sciences</subject><subject>magnesium</subject><subject>Male</subject><subject>Milk</subject><subject>Nutrition</subject><subject>phytate</subject><subject>Phytic Acid - pharmacology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Rodents</subject><subject>Small intestine</subject><subject>Vertebrates: digestive system</subject><issn>0022-3166</issn><issn>1541-6100</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10c9rFDEUB_Agit1Wj14lSCl4mO1LJj8mx7WttrCi-APBS8gmGTbLbKYmMwv735t1FpVCT4HHJy9574vQKwJzAqq-3MRLUsO8mVOg8ATNCGekEgTgKZoBUFrVRIgTdJrzBgAIU81zdFJuEqmImqGf18EPJu3x5_V-CBYvbHDYRId_rL0Z8LtkIr6JaxOtxx9H22fT_aEm-2KHsAvDHoeIvxT8dWu6Dt_FwechRP8CPWtNl_3L43mGvr-_-XZ1Wy0_fbi7Wiwry2s6VM6teCuNENY1kjUrJd1KgJO8VDgH3gpopGNgqRKCG0WdaD2jlEpWM9b6-gy9nfquTafvU9iWcXRvgr5dLPWhBlQoKRnsSLEXk71P_a-x_FNvQ7a-60z0_Zi1JFTKhvEC3zyAm35MscyhiZKME9UculUTsqnPOfn27_ME9CEdvYm6pKMbfUin-NfHpuNq691_eoqjgPMjMNmari3btyH_c7zmE5MT82Wtu-CTzjb4EpELydtBuz488oPfm9impQ</recordid><startdate>20000801</startdate><enddate>20000801</enddate><creator>Lopez, Hubert W.</creator><creator>Vallery, Franck</creator><creator>Levrat-Verny, Marie-Anne</creator><creator>Coudray, Charles</creator><creator>Demigné, Christian</creator><creator>Rémésy, Christian</creator><general>Elsevier Inc</general><general>American Society for Nutritional Sciences</general><general>American Institute of Nutrition</general><general>American Society for Nutrition</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-6295-9389</orcidid><orcidid>https://orcid.org/0000-0003-2680-7796</orcidid></search><sort><creationdate>20000801</creationdate><title>Dietary Phytic Acid and Wheat Bran Enhance Mucosal Phytase Activity in Rat Small Intestine</title><author>Lopez, Hubert W. ; Vallery, Franck ; Levrat-Verny, Marie-Anne ; Coudray, Charles ; Demigné, Christian ; Rémésy, Christian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c532t-ddb5f7a66cd8748b97db60d7566c5505f6087d40c29665a92d6fe422274344fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>6-Phytase - biosynthesis</topic><topic>absorption</topic><topic>Acids</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcium</topic><topic>degradation</topic><topic>Dietary Fiber - pharmacology</topic><topic>Enzyme Induction</topic><topic>Food and Nutrition</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycine max</topic><topic>Intestinal Mucosa - drug effects</topic><topic>Intestinal Mucosa - enzymology</topic><topic>Intestine, Small - drug effects</topic><topic>Intestine, Small - enzymology</topic><topic>Intestine. Mesentery</topic><topic>Life Sciences</topic><topic>magnesium</topic><topic>Male</topic><topic>Milk</topic><topic>Nutrition</topic><topic>phytate</topic><topic>Phytic Acid - pharmacology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Rodents</topic><topic>Small intestine</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lopez, Hubert W.</creatorcontrib><creatorcontrib>Vallery, Franck</creatorcontrib><creatorcontrib>Levrat-Verny, Marie-Anne</creatorcontrib><creatorcontrib>Coudray, Charles</creatorcontrib><creatorcontrib>Demigné, Christian</creatorcontrib><creatorcontrib>Rémésy, Christian</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>The Journal of nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lopez, Hubert W.</au><au>Vallery, Franck</au><au>Levrat-Verny, Marie-Anne</au><au>Coudray, Charles</au><au>Demigné, Christian</au><au>Rémésy, Christian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dietary Phytic Acid and Wheat Bran Enhance Mucosal Phytase Activity in Rat Small Intestine</atitle><jtitle>The Journal of nutrition</jtitle><addtitle>J Nutr</addtitle><date>2000-08-01</date><risdate>2000</risdate><volume>130</volume><issue>8</issue><spage>2020</spage><epage>2025</epage><pages>2020-2025</pages><issn>0022-3166</issn><eissn>1541-6100</eissn><coden>JONUAI</coden><abstract>The aim of this work was to investigate the influence of dietary phytic acid (PA) on intestinal phytase activity in growing rats by in vitro determination of phytase activity in the three segments of the small intestine (duodenum, jejunum and ileum), and by in vivo intestinal perfusion of a solution rich in PA (diluted soymilk). Using the in vitro method, duodenal and jejunal activities were enhanced significantly by adaptation to purified PA (+44 and +145% respectively, compared with control rats). For the rats adapted to the wheat bran (WB) diet, the induction of intestinal phytase by the substrate compared with the control values (P < 0.001) was observed only in ileum. Using soymilk in perfusions, rats consuming PA or WB diets hydrolyzed more phytate (P < 0.001 and P < 0.05, respectively) than controls. Further, Mg absorption from diluted soymilk was not affected by food adaptation, whereas Ca absorption was greater in the PA and WB groups (P < 0.001 and P < 0.05, respectively) than in the control group. Thus, intake of pure PA by rats enhances phytase in the upper parts of the small intestine (duodenum and jejunum), whereas the WB diet activates ileal phytase. Furthermore, the induction of phytase activity is greater in magnitude in rats fed synthetic PA than that observed in rats fed the WB diet. The enhancement of phytase improves intestinal Ca absorption, thus showing the capacity of the small intestine to adapt to diets rich in PA and poor in Ca.</abstract><cop>Bethesda, MD</cop><pub>Elsevier Inc</pub><pmid>10917919</pmid><doi>10.1093/jn/130.8.2020</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0001-6295-9389</orcidid><orcidid>https://orcid.org/0000-0003-2680-7796</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 6-Phytase - biosynthesis absorption Acids Animals Biological and medical sciences Calcium degradation Dietary Fiber - pharmacology Enzyme Induction Food and Nutrition Fundamental and applied biological sciences. Psychology Glycine max Intestinal Mucosa - drug effects Intestinal Mucosa - enzymology Intestine, Small - drug effects Intestine, Small - enzymology Intestine. Mesentery Life Sciences magnesium Male Milk Nutrition phytate Phytic Acid - pharmacology Rats Rats, Wistar Rodents Small intestine Vertebrates: digestive system |
| title | Dietary Phytic Acid and Wheat Bran Enhance Mucosal Phytase Activity in Rat Small Intestine |
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