Comparison of the humoral and cellular immune responses to two preparations of Cryptosporidium parvum CP15/60 recombinant protein
This study compares the immune responses produced by immunising mice and rabbits with two preparations of the recombinant 15/60 kDa protein of Cryptosporidium parvum. Genomic C. parvum DNA was amplified and the recombinant protein was synthesized as a fusion protein with glutathione-S-transferase in...
Gespeichert in:
| Veröffentlicht in: | Microbial pathogenesis 1999-06, Vol.26 (6), p.307-315 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 315 |
|---|---|
| container_issue | 6 |
| container_start_page | 307 |
| container_title | Microbial pathogenesis |
| container_volume | 26 |
| creator | Iochmann, S Sagodira, S Mevelec, M.N Reperant, J.M Naciri, M Coursaget, P Bout, D |
| description | This study compares the immune responses produced by immunising mice and rabbits with two preparations of the recombinant 15/60 kDa protein of Cryptosporidium parvum. Genomic C. parvum DNA was amplified and the recombinant protein was synthesized as a fusion protein with glutathione-S-transferase in Escherichia coli and in the eukaryotic system of baculovirus/insect cells. Both recombinant proteins induced similar levels of serum antibodies against the fusion recombinant protein, but the eukaryotic recombinant protein triggered a stronger humoral response to C. parvum. Similarly, increased lymphoproliferation occurred only after stimulation of spleen cells from mice immunised with the eukaryotic recombinant protein. This suggests that the eukaryotic protein is a better candidate for immunological studies on cryptosporidiosis. |
| doi_str_mv | 10.1006/mpat.1999.0276 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_02697374v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17232042</sourcerecordid><originalsourceid>FETCH-LOGICAL-f325t-2607b495b5b8a3786e2694248d0d2963d41e1cd30f161bd39b40299e11fce39e3</originalsourceid><addsrcrecordid>eNqFkc1v1DAQxS1ERZfClSP4gJA4ZDv-iBMfqwhopZVAgp4jJ3FYozgOttOqR_7zzmoXeuQ00szvPb2ZIeQNgy0DUJd-MXnLtNZb4JV6RjYMtCoYh_o52UBd80ICg3PyMqVfAKCl0C_IOQMhBZR6Q_40AS2iS2GmYaR5b-l-9SGaiZp5oL2dpnUykTrv19nSaNMS5mQTzYHm-0CXaFFussPuwaCJD0sOCEU3uNVTHN5hab6x8lIB6vvgOzebOaM0ZOvmV-RsNFOyr0_1gtx-_vSjuS52X7_cNFe7YhS8zAVXUHVSl13Z1UZUtbJcacllPcDAtRKDZJb1g4CRKdYNQncSuNaWsbG3QltxQT4effdmapfovIkPbTCuvb7atYceoGElKnnHkP1wZDHj79Wm3HqXDrcwsw1rahGsuZbwX5BVXHCQHMG3J3DtvB3-Bfj7CQTenwCTejON0cy9S09craoSDsneHbHRhNb8xMe1t985DnBZtKmEeARxsqDr</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17232042</pqid></control><display><type>article</type><title>Comparison of the humoral and cellular immune responses to two preparations of Cryptosporidium parvum CP15/60 recombinant protein</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Iochmann, S ; Sagodira, S ; Mevelec, M.N ; Reperant, J.M ; Naciri, M ; Coursaget, P ; Bout, D</creator><creatorcontrib>Iochmann, S ; Sagodira, S ; Mevelec, M.N ; Reperant, J.M ; Naciri, M ; Coursaget, P ; Bout, D</creatorcontrib><description>This study compares the immune responses produced by immunising mice and rabbits with two preparations of the recombinant 15/60 kDa protein of Cryptosporidium parvum. Genomic C. parvum DNA was amplified and the recombinant protein was synthesized as a fusion protein with glutathione-S-transferase in Escherichia coli and in the eukaryotic system of baculovirus/insect cells. Both recombinant proteins induced similar levels of serum antibodies against the fusion recombinant protein, but the eukaryotic recombinant protein triggered a stronger humoral response to C. parvum. Similarly, increased lymphoproliferation occurred only after stimulation of spleen cells from mice immunised with the eukaryotic recombinant protein. This suggests that the eukaryotic protein is a better candidate for immunological studies on cryptosporidiosis.</description><identifier>ISSN: 0882-4010</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1006/mpat.1999.0276</identifier><identifier>PMID: 10343059</identifier><identifier>CODEN: MIPAEV</identifier><language>eng</language><publisher>Oxford: Elsevier</publisher><subject>animal health ; animal parasites and pests ; Animals ; Antibody Formation ; Biological and medical sciences ; Cell Division ; Cell Line ; Cryptosporidium parvum - genetics ; Cryptosporidium parvum - immunology ; Drosophila Proteins ; Eukaryotic Cells ; Fundamental and applied biological sciences. Psychology ; Glutathione Transferase - genetics ; Glutathione Transferase - immunology ; human health and safety ; Immunity, Cellular ; Life Sciences ; medicine ; Mice ; Microtubule-Associated Proteins - genetics ; Microtubule-Associated Proteins - immunology ; Nuclear Proteins - genetics ; Nuclear Proteins - immunology ; Prokaryotic Cells ; Protozoa ; Protozoan Proteins - genetics ; Protozoan Proteins - immunology ; Protozoan Vaccines - immunology ; Rabbits ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - immunology ; Spleen - cytology ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Vaccines, Synthetic - immunology</subject><ispartof>Microbial pathogenesis, 1999-06, Vol.26 (6), p.307-315</ispartof><rights>1999 INIST-CNRS</rights><rights>Copyright 1999 Academic Press.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0003-2485-1603 ; 0000-0002-4877-895X ; 0000-0001-6784-8549</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1867501$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10343059$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02697374$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Iochmann, S</creatorcontrib><creatorcontrib>Sagodira, S</creatorcontrib><creatorcontrib>Mevelec, M.N</creatorcontrib><creatorcontrib>Reperant, J.M</creatorcontrib><creatorcontrib>Naciri, M</creatorcontrib><creatorcontrib>Coursaget, P</creatorcontrib><creatorcontrib>Bout, D</creatorcontrib><title>Comparison of the humoral and cellular immune responses to two preparations of Cryptosporidium parvum CP15/60 recombinant protein</title><title>Microbial pathogenesis</title><addtitle>Microb Pathog</addtitle><description>This study compares the immune responses produced by immunising mice and rabbits with two preparations of the recombinant 15/60 kDa protein of Cryptosporidium parvum. Genomic C. parvum DNA was amplified and the recombinant protein was synthesized as a fusion protein with glutathione-S-transferase in Escherichia coli and in the eukaryotic system of baculovirus/insect cells. Both recombinant proteins induced similar levels of serum antibodies against the fusion recombinant protein, but the eukaryotic recombinant protein triggered a stronger humoral response to C. parvum. Similarly, increased lymphoproliferation occurred only after stimulation of spleen cells from mice immunised with the eukaryotic recombinant protein. This suggests that the eukaryotic protein is a better candidate for immunological studies on cryptosporidiosis.</description><subject>animal health</subject><subject>animal parasites and pests</subject><subject>Animals</subject><subject>Antibody Formation</subject><subject>Biological and medical sciences</subject><subject>Cell Division</subject><subject>Cell Line</subject><subject>Cryptosporidium parvum - genetics</subject><subject>Cryptosporidium parvum - immunology</subject><subject>Drosophila Proteins</subject><subject>Eukaryotic Cells</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glutathione Transferase - genetics</subject><subject>Glutathione Transferase - immunology</subject><subject>human health and safety</subject><subject>Immunity, Cellular</subject><subject>Life Sciences</subject><subject>medicine</subject><subject>Mice</subject><subject>Microtubule-Associated Proteins - genetics</subject><subject>Microtubule-Associated Proteins - immunology</subject><subject>Nuclear Proteins - genetics</subject><subject>Nuclear Proteins - immunology</subject><subject>Prokaryotic Cells</subject><subject>Protozoa</subject><subject>Protozoan Proteins - genetics</subject><subject>Protozoan Proteins - immunology</subject><subject>Protozoan Vaccines - immunology</subject><subject>Rabbits</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - immunology</subject><subject>Spleen - cytology</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Vaccines, Synthetic - immunology</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS1ERZfClSP4gJA4ZDv-iBMfqwhopZVAgp4jJ3FYozgOttOqR_7zzmoXeuQ00szvPb2ZIeQNgy0DUJd-MXnLtNZb4JV6RjYMtCoYh_o52UBd80ICg3PyMqVfAKCl0C_IOQMhBZR6Q_40AS2iS2GmYaR5b-l-9SGaiZp5oL2dpnUykTrv19nSaNMS5mQTzYHm-0CXaFFussPuwaCJD0sOCEU3uNVTHN5hab6x8lIB6vvgOzebOaM0ZOvmV-RsNFOyr0_1gtx-_vSjuS52X7_cNFe7YhS8zAVXUHVSl13Z1UZUtbJcacllPcDAtRKDZJb1g4CRKdYNQncSuNaWsbG3QltxQT4effdmapfovIkPbTCuvb7atYceoGElKnnHkP1wZDHj79Wm3HqXDrcwsw1rahGsuZbwX5BVXHCQHMG3J3DtvB3-Bfj7CQTenwCTejON0cy9S09craoSDsneHbHRhNb8xMe1t985DnBZtKmEeARxsqDr</recordid><startdate>19990601</startdate><enddate>19990601</enddate><creator>Iochmann, S</creator><creator>Sagodira, S</creator><creator>Mevelec, M.N</creator><creator>Reperant, J.M</creator><creator>Naciri, M</creator><creator>Coursaget, P</creator><creator>Bout, D</creator><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>M7N</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-2485-1603</orcidid><orcidid>https://orcid.org/0000-0002-4877-895X</orcidid><orcidid>https://orcid.org/0000-0001-6784-8549</orcidid></search><sort><creationdate>19990601</creationdate><title>Comparison of the humoral and cellular immune responses to two preparations of Cryptosporidium parvum CP15/60 recombinant protein</title><author>Iochmann, S ; Sagodira, S ; Mevelec, M.N ; Reperant, J.M ; Naciri, M ; Coursaget, P ; Bout, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f325t-2607b495b5b8a3786e2694248d0d2963d41e1cd30f161bd39b40299e11fce39e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>animal health</topic><topic>animal parasites and pests</topic><topic>Animals</topic><topic>Antibody Formation</topic><topic>Biological and medical sciences</topic><topic>Cell Division</topic><topic>Cell Line</topic><topic>Cryptosporidium parvum - genetics</topic><topic>Cryptosporidium parvum - immunology</topic><topic>Drosophila Proteins</topic><topic>Eukaryotic Cells</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glutathione Transferase - genetics</topic><topic>Glutathione Transferase - immunology</topic><topic>human health and safety</topic><topic>Immunity, Cellular</topic><topic>Life Sciences</topic><topic>medicine</topic><topic>Mice</topic><topic>Microtubule-Associated Proteins - genetics</topic><topic>Microtubule-Associated Proteins - immunology</topic><topic>Nuclear Proteins - genetics</topic><topic>Nuclear Proteins - immunology</topic><topic>Prokaryotic Cells</topic><topic>Protozoa</topic><topic>Protozoan Proteins - genetics</topic><topic>Protozoan Proteins - immunology</topic><topic>Protozoan Vaccines - immunology</topic><topic>Rabbits</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - immunology</topic><topic>Spleen - cytology</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Vaccines, Synthetic - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Iochmann, S</creatorcontrib><creatorcontrib>Sagodira, S</creatorcontrib><creatorcontrib>Mevelec, M.N</creatorcontrib><creatorcontrib>Reperant, J.M</creatorcontrib><creatorcontrib>Naciri, M</creatorcontrib><creatorcontrib>Coursaget, P</creatorcontrib><creatorcontrib>Bout, D</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Iochmann, S</au><au>Sagodira, S</au><au>Mevelec, M.N</au><au>Reperant, J.M</au><au>Naciri, M</au><au>Coursaget, P</au><au>Bout, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of the humoral and cellular immune responses to two preparations of Cryptosporidium parvum CP15/60 recombinant protein</atitle><jtitle>Microbial pathogenesis</jtitle><addtitle>Microb Pathog</addtitle><date>1999-06-01</date><risdate>1999</risdate><volume>26</volume><issue>6</issue><spage>307</spage><epage>315</epage><pages>307-315</pages><issn>0882-4010</issn><eissn>1096-1208</eissn><coden>MIPAEV</coden><abstract>This study compares the immune responses produced by immunising mice and rabbits with two preparations of the recombinant 15/60 kDa protein of Cryptosporidium parvum. Genomic C. parvum DNA was amplified and the recombinant protein was synthesized as a fusion protein with glutathione-S-transferase in Escherichia coli and in the eukaryotic system of baculovirus/insect cells. Both recombinant proteins induced similar levels of serum antibodies against the fusion recombinant protein, but the eukaryotic recombinant protein triggered a stronger humoral response to C. parvum. Similarly, increased lymphoproliferation occurred only after stimulation of spleen cells from mice immunised with the eukaryotic recombinant protein. This suggests that the eukaryotic protein is a better candidate for immunological studies on cryptosporidiosis.</abstract><cop>Oxford</cop><pub>Elsevier</pub><pmid>10343059</pmid><doi>10.1006/mpat.1999.0276</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-2485-1603</orcidid><orcidid>https://orcid.org/0000-0002-4877-895X</orcidid><orcidid>https://orcid.org/0000-0001-6784-8549</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0882-4010 |
| ispartof | Microbial pathogenesis, 1999-06, Vol.26 (6), p.307-315 |
| issn | 0882-4010 1096-1208 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_02697374v1 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | animal health animal parasites and pests Animals Antibody Formation Biological and medical sciences Cell Division Cell Line Cryptosporidium parvum - genetics Cryptosporidium parvum - immunology Drosophila Proteins Eukaryotic Cells Fundamental and applied biological sciences. Psychology Glutathione Transferase - genetics Glutathione Transferase - immunology human health and safety Immunity, Cellular Life Sciences medicine Mice Microtubule-Associated Proteins - genetics Microtubule-Associated Proteins - immunology Nuclear Proteins - genetics Nuclear Proteins - immunology Prokaryotic Cells Protozoa Protozoan Proteins - genetics Protozoan Proteins - immunology Protozoan Vaccines - immunology Rabbits Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - immunology Spleen - cytology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccines, Synthetic - immunology |
| title | Comparison of the humoral and cellular immune responses to two preparations of Cryptosporidium parvum CP15/60 recombinant protein |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-12T16%3A12%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparison%20of%20the%20humoral%20and%20cellular%20immune%20responses%20to%20two%20preparations%20of%20Cryptosporidium%20parvum%20CP15/60%20recombinant%20protein&rft.jtitle=Microbial%20pathogenesis&rft.au=Iochmann,%20S&rft.date=1999-06-01&rft.volume=26&rft.issue=6&rft.spage=307&rft.epage=315&rft.pages=307-315&rft.issn=0882-4010&rft.eissn=1096-1208&rft.coden=MIPAEV&rft_id=info:doi/10.1006/mpat.1999.0276&rft_dat=%3Cproquest_hal_p%3E17232042%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17232042&rft_id=info:pmid/10343059&rfr_iscdi=true |