Serodiagnosis of goat warble fly infestation by Przhevalskiana silenus with a commercial ELISA kit
Cross-reactivity between Hypoderma lineatum antigen and anti-Przhevalskiana silenus antibodies has been demonstrated by an ELISA technique. To evaluate the applicability of a commercial ELISA kit for the immunodiagnosis of goat warble fly infestation, different dilutions of serum and conjugate were...
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| Veröffentlicht in: | Veterinary record 1999-06, Vol.144 (26), p.726-729 |
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| container_title | Veterinary record |
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| creator | Otranto, D. Boulard, C. Giangaspero, A. Caringella, M. P. Rimmele, D. Puccini, V. |
| description | Cross-reactivity between Hypoderma lineatum antigen and anti-Przhevalskiana silenus antibodies has been demonstrated by an ELISA technique. To evaluate the applicability of a commercial ELISA kit for the immunodiagnosis of goat warble fly infestation, different dilutions of serum and conjugate were tested, the development of antibody to P silenus in naturally infested goats was studied, and the results were compared with an ELISA technique using an antigen extracted from the first instar larvae of H lineatum. The best results were obtained with a serum dilution of 1:50; with both techniques the highest antibody concentration were recorded in October, November and December. In view of the confirmed cross-reactivity between H lineatum antigen and anti-P silenus antibodies, and the simplicity and rapidity of the assay, the commercial ELISA kit can be considered as a useful tool for the diagnosis of goat warble fly infestation. |
| doi_str_mv | 10.1136/vr.144.26.726 |
| format | Article |
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In view of the confirmed cross-reactivity between H lineatum antigen and anti-P silenus antibodies, and the simplicity and rapidity of the assay, the commercial ELISA kit can be considered as a useful tool for the diagnosis of goat warble fly infestation.</description><identifier>ISSN: 0042-4900</identifier><identifier>EISSN: 2042-7670</identifier><identifier>DOI: 10.1136/vr.144.26.726</identifier><identifier>PMID: 10423816</identifier><language>eng</language><publisher>England: BMJ Publishing Group Limited</publisher><subject>Animals ; Antibodies - analysis ; Diptera - immunology ; Diptera - parasitology ; Enzyme-Linked Immunosorbent Assay ; Goat Diseases - diagnosis ; Goat Diseases - immunology ; Goat Diseases - parasitology ; Goats ; Hypodermyiasis - diagnosis ; Hypodermyiasis - immunology ; Hypodermyiasis - veterinary ; Life Sciences ; Sensitivity and Specificity ; Serologic Tests - veterinary</subject><ispartof>Veterinary record, 1999-06, Vol.144 (26), p.726-729</ispartof><rights>British Veterinary Association. All rights reserved.</rights><rights>1999 British Veterinary Association</rights><rights>Copyright: 1999 British Veterinary Association. 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| ispartof | Veterinary record, 1999-06, Vol.144 (26), p.726-729 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Antibodies - analysis Diptera - immunology Diptera - parasitology Enzyme-Linked Immunosorbent Assay Goat Diseases - diagnosis Goat Diseases - immunology Goat Diseases - parasitology Goats Hypodermyiasis - diagnosis Hypodermyiasis - immunology Hypodermyiasis - veterinary Life Sciences Sensitivity and Specificity Serologic Tests - veterinary |
| title | Serodiagnosis of goat warble fly infestation by Przhevalskiana silenus with a commercial ELISA kit |
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