Molecular cloning of two pheromone binding proteins in the cabbage armyworm Mamestra brassicae
Two cDNA clones encoding pheromone binding proteins (PBPs) were isolated from antennal cDNA of Mamestra brassicae by reverse transcription–polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends–PCR (RACE-PCR) performed with specific primers deduced from the N-terminal sequences of t...
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| Veröffentlicht in: | Insect biochemistry and molecular biology 1998-11, Vol.28 (11), p.815-818 |
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| container_title | Insect biochemistry and molecular biology |
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| creator | Maiebeche-Coisne, M Jacquin-Joly, E Francois, M-C Nagnan-Le Meillour, P |
| description | Two cDNA clones encoding pheromone binding proteins (PBPs) were isolated from antennal cDNA of
Mamestra brassicae by reverse transcription–polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends–PCR (RACE-PCR) performed with specific primers deduced from the N-terminal sequences of two PBPs previously reported. The deduced protein sequences of the two PBPs showed a strong relationship between primary structures and functional properties of the corresponding mature proteins. |
| doi_str_mv | 10.1016/S0965-1748(98)00080-0 |
| format | Article |
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| identifier | ISSN: 0965-1748 |
| ispartof | Insect biochemistry and molecular biology, 1998-11, Vol.28 (11), p.815-818 |
| issn | 0965-1748 1879-0240 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Animals Base Sequence Biochemistry, Molecular Biology Carrier Proteins - chemistry Cloning, Molecular DNA, Complementary - chemistry Insect Proteins - chemistry Insect Proteins - genetics Lepidoptera Life Sciences Male Mamestra brassicae Molecular Sequence Data Moths - genetics Noctuidae Olfaction Pheromone binding protein Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid |
| title | Molecular cloning of two pheromone binding proteins in the cabbage armyworm Mamestra brassicae |
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