Related RNAs in Lepidopteran Cells after in Vitro Infection with Hyposoter didymator Virus Define a New Polydnavirus Gene Family
In the present study, we describe the isolation and the characterization of three different Hyposoter didymator virus (HdV) lepidopteran host-expressed genes, the products of which might interfere with the host physiology during parasitism. In this report, we study the expression of HdV genes in Sf9...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1999-10, Vol.263 (2), p.349-363 |
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| creator | Volkoff, Anne-Nathalie Cérutti, Pierre Rocher, Janick Ohresser, Marc C.P. Devauchelle, Gérard Duonor-Cérutti, Martine |
| description | In the present study, we describe the isolation and the characterization of three different Hyposoter didymator virus (HdV) lepidopteran host-expressed genes, the products of which might interfere with the host physiology during parasitism. In this report, we study the expression of HdV genes in Sf9 cells infected with HdV since results indicate that the Sf9 model mimics to some extent the in vivo model and may be utilized to study expression of HdV genes in lepidopteran host cells. This system allowed us to isolate three HdV-specific cDNAs, termed M24, M27, and M40. cDNA nucleotide sequence analysis demonstrated significant regions of homology. The three cDNAs displayed repeated sequences arranged in tandem array that might have evolved through domain duplication. Similar to other previously described polydnavirus host-expressed genes, two intron positions have been found in the M24 leader region. The cDNAs corresponded to RNAs of 1.5, 1.6, and 2.3 kb that are also detected in parasitized Spodoptera littoralis larvae. They are encoded by different genes likely located on different HdV DNA molecules. Corresponding RNAs are detected early postinfection and remain detectable for at least 10 days postinfection. They encode secreted glycine- and proline-rich proteins. An antiserum raised against a baculovirus recombinant M24-encoded protein detected similar proteins in the culture medium of infected lepidopteran cells and in parasitized host hemolymph. We propose that the three cloned genes belong to an HdV gene family specifically expressed in parasitized lepidopteran hosts. |
| doi_str_mv | 10.1006/viro.1999.9929 |
| format | Article |
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In this report, we study the expression of HdV genes in Sf9 cells infected with HdV since results indicate that the Sf9 model mimics to some extent the in vivo model and may be utilized to study expression of HdV genes in lepidopteran host cells. This system allowed us to isolate three HdV-specific cDNAs, termed M24, M27, and M40. cDNA nucleotide sequence analysis demonstrated significant regions of homology. The three cDNAs displayed repeated sequences arranged in tandem array that might have evolved through domain duplication. Similar to other previously described polydnavirus host-expressed genes, two intron positions have been found in the M24 leader region. The cDNAs corresponded to RNAs of 1.5, 1.6, and 2.3 kb that are also detected in parasitized Spodoptera littoralis larvae. They are encoded by different genes likely located on different HdV DNA molecules. Corresponding RNAs are detected early postinfection and remain detectable for at least 10 days postinfection. They encode secreted glycine- and proline-rich proteins. An antiserum raised against a baculovirus recombinant M24-encoded protein detected similar proteins in the culture medium of infected lepidopteran cells and in parasitized host hemolymph. We propose that the three cloned genes belong to an HdV gene family specifically expressed in parasitized lepidopteran hosts.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1006/viro.1999.9929</identifier><identifier>PMID: 10544108</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Cell Line ; Cloning, Molecular ; Female ; genbank/af131648 ; genbank/af132023 ; genbank/af132024 ; genbank/af156933 ; Gene Expression Regulation, Viral ; Genes, Viral - genetics ; Genome, Viral ; Hymenoptera - physiology ; Hymenoptera - virology ; Hyposoter didymator virus ; Introns - genetics ; Larva - metabolism ; Larva - parasitology ; Larva - virology ; Life Sciences ; Molecular Sequence Data ; Molecular Weight ; Noctuidae ; Open Reading Frames - genetics ; parasitism ; parasitoids ; Polydnaviridae - genetics ; Polydnaviridae - physiology ; Recombinant Proteins - chemistry ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; RNA, Messenger - analysis ; RNA, Messenger - biosynthesis ; RNA, Messenger - genetics ; RNA, Viral - analysis ; RNA, Viral - biosynthesis ; RNA, Viral - genetics ; Sequence Alignment ; Sequence Homology, Nucleic Acid ; Spodoptera - cytology ; Spodoptera - genetics ; Spodoptera - parasitology ; Spodoptera - virology ; Spodoptera littoralis ; Tandem Repeat Sequences - genetics ; Viral Proteins - chemistry ; Viral Proteins - genetics ; Viral Proteins - metabolism</subject><ispartof>Virology (New York, N.Y.), 1999-10, Vol.263 (2), p.349-363</ispartof><rights>1999 Academic Press</rights><rights>Copyright 1999 Academic Press.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c497t-e2f699046265cc9f78b9060f9c05a28833edfd117abf4d657516a5d6dc44057d3</citedby><cites>FETCH-LOGICAL-c497t-e2f699046265cc9f78b9060f9c05a28833edfd117abf4d657516a5d6dc44057d3</cites><orcidid>0000-0002-7211-1370</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0042682299999294$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10544108$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02694329$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Volkoff, Anne-Nathalie</creatorcontrib><creatorcontrib>Cérutti, Pierre</creatorcontrib><creatorcontrib>Rocher, Janick</creatorcontrib><creatorcontrib>Ohresser, Marc C.P.</creatorcontrib><creatorcontrib>Devauchelle, Gérard</creatorcontrib><creatorcontrib>Duonor-Cérutti, Martine</creatorcontrib><title>Related RNAs in Lepidopteran Cells after in Vitro Infection with Hyposoter didymator Virus Define a New Polydnavirus Gene Family</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>In the present study, we describe the isolation and the characterization of three different Hyposoter didymator virus (HdV) lepidopteran host-expressed genes, the products of which might interfere with the host physiology during parasitism. In this report, we study the expression of HdV genes in Sf9 cells infected with HdV since results indicate that the Sf9 model mimics to some extent the in vivo model and may be utilized to study expression of HdV genes in lepidopteran host cells. This system allowed us to isolate three HdV-specific cDNAs, termed M24, M27, and M40. cDNA nucleotide sequence analysis demonstrated significant regions of homology. The three cDNAs displayed repeated sequences arranged in tandem array that might have evolved through domain duplication. Similar to other previously described polydnavirus host-expressed genes, two intron positions have been found in the M24 leader region. The cDNAs corresponded to RNAs of 1.5, 1.6, and 2.3 kb that are also detected in parasitized Spodoptera littoralis larvae. They are encoded by different genes likely located on different HdV DNA molecules. Corresponding RNAs are detected early postinfection and remain detectable for at least 10 days postinfection. They encode secreted glycine- and proline-rich proteins. An antiserum raised against a baculovirus recombinant M24-encoded protein detected similar proteins in the culture medium of infected lepidopteran cells and in parasitized host hemolymph. We propose that the three cloned genes belong to an HdV gene family specifically expressed in parasitized lepidopteran hosts.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Cell Line</subject><subject>Cloning, Molecular</subject><subject>Female</subject><subject>genbank/af131648</subject><subject>genbank/af132023</subject><subject>genbank/af132024</subject><subject>genbank/af156933</subject><subject>Gene Expression Regulation, Viral</subject><subject>Genes, Viral - genetics</subject><subject>Genome, Viral</subject><subject>Hymenoptera - physiology</subject><subject>Hymenoptera - virology</subject><subject>Hyposoter didymator virus</subject><subject>Introns - genetics</subject><subject>Larva - metabolism</subject><subject>Larva - parasitology</subject><subject>Larva - virology</subject><subject>Life Sciences</subject><subject>Molecular Sequence Data</subject><subject>Molecular Weight</subject><subject>Noctuidae</subject><subject>Open Reading Frames - genetics</subject><subject>parasitism</subject><subject>parasitoids</subject><subject>Polydnaviridae - genetics</subject><subject>Polydnaviridae - physiology</subject><subject>Recombinant Proteins - chemistry</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Viral - analysis</subject><subject>RNA, Viral - biosynthesis</subject><subject>RNA, Viral - genetics</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Spodoptera - cytology</subject><subject>Spodoptera - genetics</subject><subject>Spodoptera - parasitology</subject><subject>Spodoptera - virology</subject><subject>Spodoptera littoralis</subject><subject>Tandem Repeat Sequences - genetics</subject><subject>Viral Proteins - chemistry</subject><subject>Viral Proteins - genetics</subject><subject>Viral Proteins - metabolism</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhiMEokvhyhF8QuKQZfwRJz6uFtqttCqoUK6WNx5To2y82NmtcuOn45AKcUGcrJn38diapyheUlhSAPnu5GNYUqXUUimmHhULCkqWwAV9XCwABCtlw9hZ8Syl75DruoanxRmFSggKzaL4eYOdGdCSm-tVIr4nWzx4Gw4DRtOTNXZdIsblasq--iEGctU7bAcfenLvhzuyGQ8hhYmw3o57M4SYwXhM5D063yMx5BrvyafQjbY3p9_JJeb-hdn7bnxePHGmS_ji4Twvbi8-fFlvyu3Hy6v1alu2QtVDicxJpUBIJqu2Va5udgokONVCZVjTcI7WWUprs3PCyqquqDSVlbYVAqra8vPi7Tz3znT6EP3exFEH4_VmtdVTD5hUgjN1opl9M7OHGH4cMQ1671Obd2F6DMekpWKMc179F6Q1F8Apz-ByBtsYUoro_nyBgp5E6kmknkTqSWS-8Oph8nG3R_sXPpvLwOsZcCZo8y36pG8_M6AcmBI5F5loZgLzWk8eo06tx75F62MWqG3w_3r9F5URtdU</recordid><startdate>19991025</startdate><enddate>19991025</enddate><creator>Volkoff, Anne-Nathalie</creator><creator>Cérutti, Pierre</creator><creator>Rocher, Janick</creator><creator>Ohresser, Marc C.P.</creator><creator>Devauchelle, Gérard</creator><creator>Duonor-Cérutti, Martine</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-7211-1370</orcidid></search><sort><creationdate>19991025</creationdate><title>Related RNAs in Lepidopteran Cells after in Vitro Infection with Hyposoter didymator Virus Define a New Polydnavirus Gene Family</title><author>Volkoff, Anne-Nathalie ; 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In this report, we study the expression of HdV genes in Sf9 cells infected with HdV since results indicate that the Sf9 model mimics to some extent the in vivo model and may be utilized to study expression of HdV genes in lepidopteran host cells. This system allowed us to isolate three HdV-specific cDNAs, termed M24, M27, and M40. cDNA nucleotide sequence analysis demonstrated significant regions of homology. The three cDNAs displayed repeated sequences arranged in tandem array that might have evolved through domain duplication. Similar to other previously described polydnavirus host-expressed genes, two intron positions have been found in the M24 leader region. The cDNAs corresponded to RNAs of 1.5, 1.6, and 2.3 kb that are also detected in parasitized Spodoptera littoralis larvae. They are encoded by different genes likely located on different HdV DNA molecules. Corresponding RNAs are detected early postinfection and remain detectable for at least 10 days postinfection. They encode secreted glycine- and proline-rich proteins. An antiserum raised against a baculovirus recombinant M24-encoded protein detected similar proteins in the culture medium of infected lepidopteran cells and in parasitized host hemolymph. We propose that the three cloned genes belong to an HdV gene family specifically expressed in parasitized lepidopteran hosts.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>10544108</pmid><doi>10.1006/viro.1999.9929</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-7211-1370</orcidid><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 1999-10, Vol.263 (2), p.349-363 |
| issn | 0042-6822 1096-0341 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_02694329v1 |
| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular Female genbank/af131648 genbank/af132023 genbank/af132024 genbank/af156933 Gene Expression Regulation, Viral Genes, Viral - genetics Genome, Viral Hymenoptera - physiology Hymenoptera - virology Hyposoter didymator virus Introns - genetics Larva - metabolism Larva - parasitology Larva - virology Life Sciences Molecular Sequence Data Molecular Weight Noctuidae Open Reading Frames - genetics parasitism parasitoids Polydnaviridae - genetics Polydnaviridae - physiology Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism RNA, Messenger - analysis RNA, Messenger - biosynthesis RNA, Messenger - genetics RNA, Viral - analysis RNA, Viral - biosynthesis RNA, Viral - genetics Sequence Alignment Sequence Homology, Nucleic Acid Spodoptera - cytology Spodoptera - genetics Spodoptera - parasitology Spodoptera - virology Spodoptera littoralis Tandem Repeat Sequences - genetics Viral Proteins - chemistry Viral Proteins - genetics Viral Proteins - metabolism |
| title | Related RNAs in Lepidopteran Cells after in Vitro Infection with Hyposoter didymator Virus Define a New Polydnavirus Gene Family |
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