Effect of Melatonin on the Release of Gonadotropin-Releasing Hormone and Cyclic AMP from the Rat Hypothalamus: an in vitro Study
Using an in vitro static incubation system of adult male rat hypothalami, we have studied the effect of melatonin on the release of gonadotropin‐releasing hormone (GnRH) and cyclic adenosine monophosphate (cAMP). Mediobasal hypothalamus (MBH) and preoptic area (POA) were incubated separately in Mini...
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| Veröffentlicht in: | Journal of neuroendocrinology 1996-10, Vol.8 (10), p.801-807 |
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| creator | Messager, S. Caillol, M. Rossano, B. Martinet, L. |
| description | Using an in vitro static incubation system of adult male rat hypothalami, we have studied the effect of melatonin on the release of gonadotropin‐releasing hormone (GnRH) and cyclic adenosine monophosphate (cAMP). Mediobasal hypothalamus (MBH) and preoptic area (POA) were incubated separately in Minimum Essential Medium (MEM) for 6 h. The release of GnRH was measured by radioimmunoassay in the incubation medium sampled every 7.5 min. In the MBH and POA incubation medium, the mean amount of GnRH released was 8.9±1.1 and 3.4±0.6 pg GnRH/7.5 min, respectively (P0.05). Melatonin (10−8 M) did not alter the release of GnRH in the presence or absence of forskolin (10−4 M). Melatonin, which was without effect on basal cAMP, inhibited forskolin‐stimulated cAMP accumulation in the medium by 50% in the MBH and 40% in the POA. These results suggest that in our incubation system, melatonin does not modify GnRH release, but probably acts through the melatonin binding sites located in the hypothalamus to inhibit forskolin‐stimulated cAMP. |
| doi_str_mv | 10.1046/j.1365-2826.1996.52711.x |
| format | Article |
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Mediobasal hypothalamus (MBH) and preoptic area (POA) were incubated separately in Minimum Essential Medium (MEM) for 6 h. The release of GnRH was measured by radioimmunoassay in the incubation medium sampled every 7.5 min. In the MBH and POA incubation medium, the mean amount of GnRH released was 8.9±1.1 and 3.4±0.6 pg GnRH/7.5 min, respectively (P<0.01). The mean number of GnRH pulses under basal conditions was 2±0.3 per 2 h in the MBH and 1.6±0.3 per 2 h in the POA (P>0.05). Melatonin (10−8 M) did not alter the release of GnRH in the presence or absence of forskolin (10−4 M). Melatonin, which was without effect on basal cAMP, inhibited forskolin‐stimulated cAMP accumulation in the medium by 50% in the MBH and 40% in the POA. These results suggest that in our incubation system, melatonin does not modify GnRH release, but probably acts through the melatonin binding sites located in the hypothalamus to inhibit forskolin‐stimulated cAMP.</description><identifier>ISSN: 0953-8194</identifier><identifier>EISSN: 1365-2826</identifier><identifier>DOI: 10.1046/j.1365-2826.1996.52711.x</identifier><identifier>PMID: 8910811</identifier><language>eng</language><publisher>Oxford: Blackwell Science Ltd</publisher><subject>Animals ; Colforsin - pharmacology ; cyclic AMP ; Cyclic AMP - secretion ; GnRH ; Gonadotropin-Releasing Hormone - secretion ; hypothalamus ; Hypothalamus, Middle - drug effects ; Hypothalamus, Middle - secretion ; in vitro ; In Vitro Techniques ; Life Sciences ; Male ; melatonin ; Melatonin - pharmacology ; Preoptic Area - drug effects ; Preoptic Area - secretion ; Rats ; Rats, Wistar ; Reproducibility of Results</subject><ispartof>Journal of neuroendocrinology, 1996-10, Vol.8 (10), p.801-807</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4357-4a667bfb9efa8949267fae952fe1324acb580d6e749f63c43226f9c9d12f87193</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2826.1996.52711.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2826.1996.52711.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8910811$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02693711$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Messager, S.</creatorcontrib><creatorcontrib>Caillol, M.</creatorcontrib><creatorcontrib>Rossano, B.</creatorcontrib><creatorcontrib>Martinet, L.</creatorcontrib><title>Effect of Melatonin on the Release of Gonadotropin-Releasing Hormone and Cyclic AMP from the Rat Hypothalamus: an in vitro Study</title><title>Journal of neuroendocrinology</title><addtitle>J Neuroendocrinol</addtitle><description>Using an in vitro static incubation system of adult male rat hypothalami, we have studied the effect of melatonin on the release of gonadotropin‐releasing hormone (GnRH) and cyclic adenosine monophosphate (cAMP). Mediobasal hypothalamus (MBH) and preoptic area (POA) were incubated separately in Minimum Essential Medium (MEM) for 6 h. The release of GnRH was measured by radioimmunoassay in the incubation medium sampled every 7.5 min. In the MBH and POA incubation medium, the mean amount of GnRH released was 8.9±1.1 and 3.4±0.6 pg GnRH/7.5 min, respectively (P<0.01). The mean number of GnRH pulses under basal conditions was 2±0.3 per 2 h in the MBH and 1.6±0.3 per 2 h in the POA (P>0.05). Melatonin (10−8 M) did not alter the release of GnRH in the presence or absence of forskolin (10−4 M). Melatonin, which was without effect on basal cAMP, inhibited forskolin‐stimulated cAMP accumulation in the medium by 50% in the MBH and 40% in the POA. These results suggest that in our incubation system, melatonin does not modify GnRH release, but probably acts through the melatonin binding sites located in the hypothalamus to inhibit forskolin‐stimulated cAMP.</description><subject>Animals</subject><subject>Colforsin - pharmacology</subject><subject>cyclic AMP</subject><subject>Cyclic AMP - secretion</subject><subject>GnRH</subject><subject>Gonadotropin-Releasing Hormone - secretion</subject><subject>hypothalamus</subject><subject>Hypothalamus, Middle - drug effects</subject><subject>Hypothalamus, Middle - secretion</subject><subject>in vitro</subject><subject>In Vitro Techniques</subject><subject>Life Sciences</subject><subject>Male</subject><subject>melatonin</subject><subject>Melatonin - pharmacology</subject><subject>Preoptic Area - drug effects</subject><subject>Preoptic Area - secretion</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reproducibility of Results</subject><issn>0953-8194</issn><issn>1365-2826</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcuO0zAUhi0EGsrAIyB5hcQiGV8SXxCbqpQG1BkQDEJiY7mJzbgkcYmTmWbHo-OQqmtWts5_OZY_ACBGKUYZu9qnmLI8IYKwFEvJ0pxwjNPjI7A4C4_BAsmcJgLL7Cl4FsIeIcxzii7AhZAYCYwX4M_aWlP20Ft4bWrd-9a10LewvzPwi6mNDmbSNr7Vle87f3BtMs9d-xMWvmt8a6BuK7gay9qVcHn9GdrON3OD7mExHnx_p2vdDOFNdMK44N7FKvi1H6rxOXhidR3Mi9N5Cb69X9-uimT7afNhtdwmZUZznmSaMb6zO2msFjKThHGrjcyJNZiSTJe7XKCKGZ5Jy2jMEMKsLGWFiRUcS3oJXs-98Snq0LlGd6Py2qliuVXTDBEmafzEexy9r2bvofO_BxN61bhQmrrWrfFDUFzkWEiKolHMxrLzIXTGnpsxUhMotVcTDzXxUBMo9Q-UOsboy9OOYdeY6hw8kYn621l_cLUZ_7tXfbxZx2uMJ3Pchd4cz3Hd_VKMU56r7zcbVXD-7nYlhPpB_wKx2a_T</recordid><startdate>199610</startdate><enddate>199610</enddate><creator>Messager, S.</creator><creator>Caillol, M.</creator><creator>Rossano, B.</creator><creator>Martinet, L.</creator><general>Blackwell Science Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>199610</creationdate><title>Effect of Melatonin on the Release of Gonadotropin-Releasing Hormone and Cyclic AMP from the Rat Hypothalamus: an in vitro Study</title><author>Messager, S. ; Caillol, M. ; Rossano, B. ; Martinet, L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4357-4a667bfb9efa8949267fae952fe1324acb580d6e749f63c43226f9c9d12f87193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Colforsin - pharmacology</topic><topic>cyclic AMP</topic><topic>Cyclic AMP - secretion</topic><topic>GnRH</topic><topic>Gonadotropin-Releasing Hormone - secretion</topic><topic>hypothalamus</topic><topic>Hypothalamus, Middle - drug effects</topic><topic>Hypothalamus, Middle - secretion</topic><topic>in vitro</topic><topic>In Vitro Techniques</topic><topic>Life Sciences</topic><topic>Male</topic><topic>melatonin</topic><topic>Melatonin - pharmacology</topic><topic>Preoptic Area - drug effects</topic><topic>Preoptic Area - secretion</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reproducibility of Results</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Messager, S.</creatorcontrib><creatorcontrib>Caillol, M.</creatorcontrib><creatorcontrib>Rossano, B.</creatorcontrib><creatorcontrib>Martinet, L.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of neuroendocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Messager, S.</au><au>Caillol, M.</au><au>Rossano, B.</au><au>Martinet, L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Melatonin on the Release of Gonadotropin-Releasing Hormone and Cyclic AMP from the Rat Hypothalamus: an in vitro Study</atitle><jtitle>Journal of neuroendocrinology</jtitle><addtitle>J Neuroendocrinol</addtitle><date>1996-10</date><risdate>1996</risdate><volume>8</volume><issue>10</issue><spage>801</spage><epage>807</epage><pages>801-807</pages><issn>0953-8194</issn><eissn>1365-2826</eissn><abstract>Using an in vitro static incubation system of adult male rat hypothalami, we have studied the effect of melatonin on the release of gonadotropin‐releasing hormone (GnRH) and cyclic adenosine monophosphate (cAMP). Mediobasal hypothalamus (MBH) and preoptic area (POA) were incubated separately in Minimum Essential Medium (MEM) for 6 h. The release of GnRH was measured by radioimmunoassay in the incubation medium sampled every 7.5 min. In the MBH and POA incubation medium, the mean amount of GnRH released was 8.9±1.1 and 3.4±0.6 pg GnRH/7.5 min, respectively (P<0.01). The mean number of GnRH pulses under basal conditions was 2±0.3 per 2 h in the MBH and 1.6±0.3 per 2 h in the POA (P>0.05). Melatonin (10−8 M) did not alter the release of GnRH in the presence or absence of forskolin (10−4 M). Melatonin, which was without effect on basal cAMP, inhibited forskolin‐stimulated cAMP accumulation in the medium by 50% in the MBH and 40% in the POA. These results suggest that in our incubation system, melatonin does not modify GnRH release, but probably acts through the melatonin binding sites located in the hypothalamus to inhibit forskolin‐stimulated cAMP.</abstract><cop>Oxford</cop><pub>Blackwell Science Ltd</pub><pmid>8910811</pmid><doi>10.1046/j.1365-2826.1996.52711.x</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of neuroendocrinology, 1996-10, Vol.8 (10), p.801-807 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Colforsin - pharmacology cyclic AMP Cyclic AMP - secretion GnRH Gonadotropin-Releasing Hormone - secretion hypothalamus Hypothalamus, Middle - drug effects Hypothalamus, Middle - secretion in vitro In Vitro Techniques Life Sciences Male melatonin Melatonin - pharmacology Preoptic Area - drug effects Preoptic Area - secretion Rats Rats, Wistar Reproducibility of Results |
| title | Effect of Melatonin on the Release of Gonadotropin-Releasing Hormone and Cyclic AMP from the Rat Hypothalamus: an in vitro Study |
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