Identification of 16 chicken microchromosomes by molecular markers using two-colour fluorescence in situ hybridization (FISH)
A feature of avian karyotypes is the presence of microchromosomes. As a typical avian genome, the chicken karyotype (2n = 78) consists of nine pairs of macrochromosomes, including the W and Z sexual chromosomes, and 30 pairs of indistinguishable microchromosomes usually ordered arbitrarily by decrea...
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| Veröffentlicht in: | Chromosome research 1998-06, Vol.6 (4), p.307-313 |
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| creator | Fillon, V Morisson, M Zoorob, R Auffray, C Douaire, M Gellin, J Vignal, A |
| description | A feature of avian karyotypes is the presence of microchromosomes. As a typical avian genome, the chicken karyotype (2n = 78) consists of nine pairs of macrochromosomes, including the W and Z sexual chromosomes, and 30 pairs of indistinguishable microchromosomes usually ordered arbitrarily by decreasing size. Despite their reduced size, microchromosomes represent one-third of the genome and have a high gene density. So as to provide a tool to identify them, we developed a set of large insert-containing clones to be used as tags in two-colour fluorescence in situ hybridization experiments. Seventeen clones, six of which contain a microsatellite sequence and two others the fatty acid synthase gene or genes from the major histocompatibility complex, all presenting a strong hybridization signal, were selected for this purpose and enabled us to identify 16 different microchromosomes. The ability to recognize individual microchromosomes will be of great value for cytogenetic gene mapping, assignation of linkage groups from genetic maps and other studies on avian genome structure. |
| doi_str_mv | 10.1023/A:1009274925051 |
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As a typical avian genome, the chicken karyotype (2n = 78) consists of nine pairs of macrochromosomes, including the W and Z sexual chromosomes, and 30 pairs of indistinguishable microchromosomes usually ordered arbitrarily by decreasing size. Despite their reduced size, microchromosomes represent one-third of the genome and have a high gene density. So as to provide a tool to identify them, we developed a set of large insert-containing clones to be used as tags in two-colour fluorescence in situ hybridization experiments. Seventeen clones, six of which contain a microsatellite sequence and two others the fatty acid synthase gene or genes from the major histocompatibility complex, all presenting a strong hybridization signal, were selected for this purpose and enabled us to identify 16 different microchromosomes. The ability to recognize individual microchromosomes will be of great value for cytogenetic gene mapping, assignation of linkage groups from genetic maps and other studies on avian genome structure.</description><identifier>ISSN: 0967-3849</identifier><identifier>EISSN: 1573-6849</identifier><identifier>DOI: 10.1023/A:1009274925051</identifier><identifier>PMID: 9688521</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Animals ; Chickens - genetics ; Chromosome Mapping - methods ; Chromosomes - classification ; Chromosomes - genetics ; Cloning, Molecular ; DNA Probes ; Fatty Acid Synthases - genetics ; Fluorescent Dyes ; Genes, MHC Class II ; Genetic Markers ; In Situ Hybridization, Fluorescence ; Indoles ; Karyotyping - methods ; Life Sciences ; Microsatellite Repeats</subject><ispartof>Chromosome research, 1998-06, Vol.6 (4), p.307-313</ispartof><rights>Kluwer Academic Publishers 1998</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c313t-6cc30d28bf4826b005e7b4ff7ce5acfada879c8b9a708a914c4b96cc941232f3</citedby><orcidid>0000-0002-6797-2125 ; 0000-0003-3289-7417 ; 0000-0002-5279-6675</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9688521$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02693344$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Fillon, V</creatorcontrib><creatorcontrib>Morisson, M</creatorcontrib><creatorcontrib>Zoorob, R</creatorcontrib><creatorcontrib>Auffray, C</creatorcontrib><creatorcontrib>Douaire, M</creatorcontrib><creatorcontrib>Gellin, J</creatorcontrib><creatorcontrib>Vignal, A</creatorcontrib><title>Identification of 16 chicken microchromosomes by molecular markers using two-colour fluorescence in situ hybridization (FISH)</title><title>Chromosome research</title><addtitle>Chromosome Res</addtitle><description>A feature of avian karyotypes is the presence of microchromosomes. As a typical avian genome, the chicken karyotype (2n = 78) consists of nine pairs of macrochromosomes, including the W and Z sexual chromosomes, and 30 pairs of indistinguishable microchromosomes usually ordered arbitrarily by decreasing size. Despite their reduced size, microchromosomes represent one-third of the genome and have a high gene density. So as to provide a tool to identify them, we developed a set of large insert-containing clones to be used as tags in two-colour fluorescence in situ hybridization experiments. Seventeen clones, six of which contain a microsatellite sequence and two others the fatty acid synthase gene or genes from the major histocompatibility complex, all presenting a strong hybridization signal, were selected for this purpose and enabled us to identify 16 different microchromosomes. The ability to recognize individual microchromosomes will be of great value for cytogenetic gene mapping, assignation of linkage groups from genetic maps and other studies on avian genome structure.</description><subject>Animals</subject><subject>Chickens - genetics</subject><subject>Chromosome Mapping - methods</subject><subject>Chromosomes - classification</subject><subject>Chromosomes - genetics</subject><subject>Cloning, Molecular</subject><subject>DNA Probes</subject><subject>Fatty Acid Synthases - genetics</subject><subject>Fluorescent Dyes</subject><subject>Genes, MHC Class II</subject><subject>Genetic Markers</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Indoles</subject><subject>Karyotyping - methods</subject><subject>Life Sciences</subject><subject>Microsatellite Repeats</subject><issn>0967-3849</issn><issn>1573-6849</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkM1LxDAQxYMoun6cPQnBg-ihOvlom3hbRN2FBQ96L2mauNG20aRVVvB_N7KLB08zDL-ZN-8hdEzgkgBlV9NrAiBpySXNISdbaELykmWF4HIbTUAWZcZSv4f2Y3wBAME42UW7shAip2SCvueN6QdnnVaD8z32FpMC66XTr6bHndPB62XwnY--MxHXK9z51uixVQF3KryaEPEYXf-Mh0-fad_6MWDbjj6YqE2vDXY9jm4Y8XJVB9e4r7XO-d38cXZxiHasaqM52tQD9HR3-3QzyxYP9_Ob6SLTjLAhK7Rm0FBRWy5oUQPkpqy5taU2udJWNUqUUotaqhKEkoRrXsu0JDmhjFp2gC7WZ5eqrd6CS4-vKq9cNZsuqt8Z0EIyxvkHSezZmn0L_n00cag6l5y0reqNH2MlAHgKr0jg6T_wJXnvk42qzEFwkUuRoJMNNNadaf7EN_mzH1bwiC4</recordid><startdate>19980601</startdate><enddate>19980601</enddate><creator>Fillon, V</creator><creator>Morisson, M</creator><creator>Zoorob, R</creator><creator>Auffray, C</creator><creator>Douaire, M</creator><creator>Gellin, J</creator><creator>Vignal, A</creator><general>Springer Nature B.V</general><general>Springer Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-6797-2125</orcidid><orcidid>https://orcid.org/0000-0003-3289-7417</orcidid><orcidid>https://orcid.org/0000-0002-5279-6675</orcidid></search><sort><creationdate>19980601</creationdate><title>Identification of 16 chicken microchromosomes by molecular markers using two-colour fluorescence in situ hybridization (FISH)</title><author>Fillon, V ; Morisson, M ; Zoorob, R ; Auffray, C ; Douaire, M ; Gellin, J ; Vignal, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c313t-6cc30d28bf4826b005e7b4ff7ce5acfada879c8b9a708a914c4b96cc941232f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Animals</topic><topic>Chickens - genetics</topic><topic>Chromosome Mapping - methods</topic><topic>Chromosomes - classification</topic><topic>Chromosomes - genetics</topic><topic>Cloning, Molecular</topic><topic>DNA Probes</topic><topic>Fatty Acid Synthases - genetics</topic><topic>Fluorescent Dyes</topic><topic>Genes, MHC Class II</topic><topic>Genetic Markers</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Indoles</topic><topic>Karyotyping - methods</topic><topic>Life Sciences</topic><topic>Microsatellite Repeats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fillon, V</creatorcontrib><creatorcontrib>Morisson, M</creatorcontrib><creatorcontrib>Zoorob, R</creatorcontrib><creatorcontrib>Auffray, C</creatorcontrib><creatorcontrib>Douaire, M</creatorcontrib><creatorcontrib>Gellin, J</creatorcontrib><creatorcontrib>Vignal, A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Chromosome research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fillon, V</au><au>Morisson, M</au><au>Zoorob, R</au><au>Auffray, C</au><au>Douaire, M</au><au>Gellin, J</au><au>Vignal, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of 16 chicken microchromosomes by molecular markers using two-colour fluorescence in situ hybridization (FISH)</atitle><jtitle>Chromosome research</jtitle><addtitle>Chromosome Res</addtitle><date>1998-06-01</date><risdate>1998</risdate><volume>6</volume><issue>4</issue><spage>307</spage><epage>313</epage><pages>307-313</pages><issn>0967-3849</issn><eissn>1573-6849</eissn><abstract>A feature of avian karyotypes is the presence of microchromosomes. 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The ability to recognize individual microchromosomes will be of great value for cytogenetic gene mapping, assignation of linkage groups from genetic maps and other studies on avian genome structure.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>9688521</pmid><doi>10.1023/A:1009274925051</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-6797-2125</orcidid><orcidid>https://orcid.org/0000-0003-3289-7417</orcidid><orcidid>https://orcid.org/0000-0002-5279-6675</orcidid></addata></record> |
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| ispartof | Chromosome research, 1998-06, Vol.6 (4), p.307-313 |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Chickens - genetics Chromosome Mapping - methods Chromosomes - classification Chromosomes - genetics Cloning, Molecular DNA Probes Fatty Acid Synthases - genetics Fluorescent Dyes Genes, MHC Class II Genetic Markers In Situ Hybridization, Fluorescence Indoles Karyotyping - methods Life Sciences Microsatellite Repeats |
| title | Identification of 16 chicken microchromosomes by molecular markers using two-colour fluorescence in situ hybridization (FISH) |
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