Simultaneous detection of X- and Y-bearing bull spermatozoa by double colour fluorescence in situ hybridization

Double colour fluorescence in situ hybridization with sex chromosome probes was applied on sperm cells of five Swedish Holstein‐Friesian bulls. It was demonstrated that cosmids with strong fluorescence signals and scraped chromosomes can successfully be used as markers in this type of study. X and Y...

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Veröffentlicht in:	Molecular reproduction and development 1999-08, Vol.53 (4), p.407-412
Hauptverfasser:	Hassanane, M, Kovacs, A, Laurent, P, Lindblad, K, Gustavsson, I
Format:	Artikel
Sprache:	eng
Schlagworte:	Aneuploidy Animals Biological and medical sciences bull bulls Cattle cosmids detection FISH fluorescence Fundamental and applied biological sciences. Psychology In Situ Hybridization, Fluorescence - methods In Situ Hybridization, Fluorescence - veterinary Life Sciences Male Mammalian reproduction. General aspects Microscopy, Fluorescence Molecular Probes nondisjunction nucleic acid hybridization spermatozoa Spermatozoa - ultrastructure Vertebrates: reproduction X chromosome X Chromosome - genetics Y chromosome Y Chromosome - genetics
Online-Zugang:	Volltext
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