Use of a subtractive hybridization approach to identify new Medicago truncatula genes induced during root nodule development

We report the identification of new molecular markers associated with different stages of Rhizobium-induced nodule development in the legume Medicago truncatula. A cDNA library was constructed from pre-nitrogen-fixing M. truncatula nodules, and differentially screened with a polymerase chain reactio...

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Veröffentlicht in:	Molecular plant-microbe interactions 1996, Vol.9 (4), p.233-242
Hauptverfasser:	GAMAS, P, DE CARVALHO NIEBEL, F, LESCURE, N, CULLIMORE, J. V
Format:	Artikel
Sprache:	eng
Schlagworte:	adn Agronomy. Soil science and plant productions Amino Acid Sequence analytical methods arn mensajero arn messager Base Sequence Biochemistry, Molecular Biology Biological and medical sciences Blotting, Northern - methods chemical composition Cloning, Molecular composicion quimica composition chimique dna DNA, Complementary Economic plant physiology expresion genica expression des genes formation de nodosites Fundamental and applied biological sciences. Psychology gene gene expression genes identificacion identification Life Sciences Medicago sativa - genetics medicago truncatula Membrane Proteins messenger rna Molecular Sequence Data Nitrogen Fixation nodosite racinaire nodulacion nucleotide sequence nudosidades radiculares Plant Proteins - genetics Plant Roots - growth & development proteinas proteine proteins root nodulation root nodules secuencia nucleotidica sequence nucleotidique Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...) technique analytique tecnicas analiticas
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