Use of a subtractive hybridization approach to identify new Medicago truncatula genes induced during root nodule development
We report the identification of new molecular markers associated with different stages of Rhizobium-induced nodule development in the legume Medicago truncatula. A cDNA library was constructed from pre-nitrogen-fixing M. truncatula nodules, and differentially screened with a polymerase chain reactio...
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| Veröffentlicht in: | Molecular plant-microbe interactions 1996, Vol.9 (4), p.233-242 |
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| creator | GAMAS, P DE CARVALHO NIEBEL, F LESCURE, N CULLIMORE, J. V |
| description | We report the identification of new molecular markers associated with different stages of Rhizobium-induced nodule development in the legume Medicago truncatula. A cDNA library was constructed from pre-nitrogen-fixing M. truncatula nodules, and differentially screened with a polymerase chain reaction-amplified subtracted probe. Twenty-nine new families of nodulin cDNA clones, designated MtN1 to MtN29, were thus identified in addition to clones for several known nodulins. All MtN genes were shown by Northern (RNA) hybridization analysis to be induced during nodulation, some of them well before nodule emergence. The MtN genes were classified into three groups depending on their expression kinetics. The expression of three MtN genes showed a limited induction by Nod factors purified from Rhizobium meliloti. Homologies with a variety of proteins were found for the deduced amino acid sequences of 10 of the MtN genes. |
| doi_str_mv | 10.1094/MPMI-9-0233 |
| format | Article |
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The expression of three MtN genes showed a limited induction by Nod factors purified from Rhizobium meliloti. Homologies with a variety of proteins were found for the deduced amino acid sequences of 10 of the MtN genes.</description><identifier>ISSN: 0894-0282</identifier><identifier>EISSN: 1943-7706</identifier><identifier>DOI: 10.1094/MPMI-9-0233</identifier><identifier>PMID: 8634476</identifier><identifier>CODEN: MPMIEL</identifier><language>eng</language><publisher>St Paul, MN: APS Press</publisher><subject>adn ; Agronomy. Soil science and plant productions ; Amino Acid Sequence ; analytical methods ; arn mensajero ; arn messager ; Base Sequence ; Biochemistry, Molecular Biology ; Biological and medical sciences ; Blotting, Northern - methods ; chemical composition ; Cloning, Molecular ; composicion quimica ; composition chimique ; dna ; DNA, Complementary ; Economic plant physiology ; expresion genica ; expression des genes ; formation de nodosites ; Fundamental and applied biological sciences. Psychology ; gene ; gene expression ; genes ; identificacion ; identification ; Life Sciences ; Medicago sativa - genetics ; medicago truncatula ; Membrane Proteins ; messenger rna ; Molecular Sequence Data ; Nitrogen Fixation ; nodosite racinaire ; nodulacion ; nucleotide sequence ; nudosidades radiculares ; Plant Proteins - genetics ; Plant Roots - growth & development ; proteinas ; proteine ; proteins ; root nodulation ; root nodules ; secuencia nucleotidica ; sequence nucleotidique ; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...) ; technique analytique ; tecnicas analiticas</subject><ispartof>Molecular plant-microbe interactions, 1996, Vol.9 (4), p.233-242</ispartof><rights>1996 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-5db94de769523469a1e354f7d3231d14872ede6c3376464b4849df502a0a89d43</citedby><orcidid>0000-0002-5596-9420 ; 0000-0001-8804-4849</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,777,781,882,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3077164$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8634476$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02690223$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>GAMAS, P</creatorcontrib><creatorcontrib>DE CARVALHO NIEBEL, F</creatorcontrib><creatorcontrib>LESCURE, N</creatorcontrib><creatorcontrib>CULLIMORE, J. V</creatorcontrib><creatorcontrib>INRA-CNRS, Castanet-Tolosan, France</creatorcontrib><creatorcontrib>Estonian Academic Agricultural Society, Tartu (Estonia)</creatorcontrib><title>Use of a subtractive hybridization approach to identify new Medicago truncatula genes induced during root nodule development</title><title>Molecular plant-microbe interactions</title><addtitle>Mol Plant Microbe Interact</addtitle><description>We report the identification of new molecular markers associated with different stages of Rhizobium-induced nodule development in the legume Medicago truncatula. A cDNA library was constructed from pre-nitrogen-fixing M. truncatula nodules, and differentially screened with a polymerase chain reaction-amplified subtracted probe. Twenty-nine new families of nodulin cDNA clones, designated MtN1 to MtN29, were thus identified in addition to clones for several known nodulins. All MtN genes were shown by Northern (RNA) hybridization analysis to be induced during nodulation, some of them well before nodule emergence. The MtN genes were classified into three groups depending on their expression kinetics. The expression of three MtN genes showed a limited induction by Nod factors purified from Rhizobium meliloti. Homologies with a variety of proteins were found for the deduced amino acid sequences of 10 of the MtN genes.</description><subject>adn</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Amino Acid Sequence</subject><subject>analytical methods</subject><subject>arn mensajero</subject><subject>arn messager</subject><subject>Base Sequence</subject><subject>Biochemistry, Molecular Biology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern - methods</subject><subject>chemical composition</subject><subject>Cloning, Molecular</subject><subject>composicion quimica</subject><subject>composition chimique</subject><subject>dna</subject><subject>DNA, Complementary</subject><subject>Economic plant physiology</subject><subject>expresion genica</subject><subject>expression des genes</subject><subject>formation de nodosites</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene</subject><subject>gene expression</subject><subject>genes</subject><subject>identificacion</subject><subject>identification</subject><subject>Life Sciences</subject><subject>Medicago sativa - genetics</subject><subject>medicago truncatula</subject><subject>Membrane Proteins</subject><subject>messenger rna</subject><subject>Molecular Sequence Data</subject><subject>Nitrogen Fixation</subject><subject>nodosite racinaire</subject><subject>nodulacion</subject><subject>nucleotide sequence</subject><subject>nudosidades radiculares</subject><subject>Plant Proteins - genetics</subject><subject>Plant Roots - growth & development</subject><subject>proteinas</subject><subject>proteine</subject><subject>proteins</subject><subject>root nodulation</subject><subject>root nodules</subject><subject>secuencia nucleotidica</subject><subject>sequence nucleotidique</subject><subject>Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)</subject><subject>technique analytique</subject><subject>tecnicas analiticas</subject><issn>0894-0282</issn><issn>1943-7706</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kVGLEzEUhQdR1u7qk89KHpYFkdFkkkkmj8ui7kKLgvY53EnutJHppCaZSsUf7wwtfbpw7scHh1MUbxj9yKgWn1bfV0-lLmnF-bNiwbTgpVJUPi8WtNFiypvqZXGd0i9KmZZ1fVVcNZILoeSi-LdOSEJHgKSxzRFs9gck22MbvfN_IfswENjvYwC7JTkQ73DIvjuSAf-QFTpvYRNIjuNgIY89kA0OmIgf3GjRETdGP2xIDCGTIbixR-LwgH3Y7ybPq-JFB33C1-d7U6y_fP758Fguv319erhfllawKpe1a7VwqKSuKy6kBoa8Fp1yvOLMMdGoCh1Ky7mSQopWNEK7rqYVUGi0E_ymeH_ybqE3--h3EI8mgDeP90szZ7SSmlYVP7CJvTuxU-ffI6Zsdj5Z7HsYMIzJqIZyKZoZ_HACbQwpRewuZkbNvIuZdzHazLtM9Nuzdmx36C7seYjpf3v-Q7LQdxEG69MF41QpJucm705YB8HAJk7I-gfTWlE6dWCK_wd7AJ6q</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>GAMAS, P</creator><creator>DE CARVALHO NIEBEL, F</creator><creator>LESCURE, N</creator><creator>CULLIMORE, J. V</creator><general>APS Press</general><general>American Phytopathological Society</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-5596-9420</orcidid><orcidid>https://orcid.org/0000-0001-8804-4849</orcidid></search><sort><creationdate>1996</creationdate><title>Use of a subtractive hybridization approach to identify new Medicago truncatula genes induced during root nodule development</title><author>GAMAS, P ; DE CARVALHO NIEBEL, F ; LESCURE, N ; CULLIMORE, J. V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-5db94de769523469a1e354f7d3231d14872ede6c3376464b4849df502a0a89d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>adn</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Amino Acid Sequence</topic><topic>analytical methods</topic><topic>arn mensajero</topic><topic>arn messager</topic><topic>Base Sequence</topic><topic>Biochemistry, Molecular Biology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern - methods</topic><topic>chemical composition</topic><topic>Cloning, Molecular</topic><topic>composicion quimica</topic><topic>composition chimique</topic><topic>dna</topic><topic>DNA, Complementary</topic><topic>Economic plant physiology</topic><topic>expresion genica</topic><topic>expression des genes</topic><topic>formation de nodosites</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene</topic><topic>gene expression</topic><topic>genes</topic><topic>identificacion</topic><topic>identification</topic><topic>Life Sciences</topic><topic>Medicago sativa - genetics</topic><topic>medicago truncatula</topic><topic>Membrane Proteins</topic><topic>messenger rna</topic><topic>Molecular Sequence Data</topic><topic>Nitrogen Fixation</topic><topic>nodosite racinaire</topic><topic>nodulacion</topic><topic>nucleotide sequence</topic><topic>nudosidades radiculares</topic><topic>Plant Proteins - genetics</topic><topic>Plant Roots - growth & development</topic><topic>proteinas</topic><topic>proteine</topic><topic>proteins</topic><topic>root nodulation</topic><topic>root nodules</topic><topic>secuencia nucleotidica</topic><topic>sequence nucleotidique</topic><topic>Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)</topic><topic>technique analytique</topic><topic>tecnicas analiticas</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GAMAS, P</creatorcontrib><creatorcontrib>DE CARVALHO NIEBEL, F</creatorcontrib><creatorcontrib>LESCURE, N</creatorcontrib><creatorcontrib>CULLIMORE, J. V</creatorcontrib><creatorcontrib>INRA-CNRS, Castanet-Tolosan, France</creatorcontrib><creatorcontrib>Estonian Academic Agricultural Society, Tartu (Estonia)</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Molecular plant-microbe interactions</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GAMAS, P</au><au>DE CARVALHO NIEBEL, F</au><au>LESCURE, N</au><au>CULLIMORE, J. V</au><aucorp>INRA-CNRS, Castanet-Tolosan, France</aucorp><aucorp>Estonian Academic Agricultural Society, Tartu (Estonia)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Use of a subtractive hybridization approach to identify new Medicago truncatula genes induced during root nodule development</atitle><jtitle>Molecular plant-microbe interactions</jtitle><addtitle>Mol Plant Microbe Interact</addtitle><date>1996</date><risdate>1996</risdate><volume>9</volume><issue>4</issue><spage>233</spage><epage>242</epage><pages>233-242</pages><issn>0894-0282</issn><eissn>1943-7706</eissn><coden>MPMIEL</coden><abstract>We report the identification of new molecular markers associated with different stages of Rhizobium-induced nodule development in the legume Medicago truncatula. A cDNA library was constructed from pre-nitrogen-fixing M. truncatula nodules, and differentially screened with a polymerase chain reaction-amplified subtracted probe. Twenty-nine new families of nodulin cDNA clones, designated MtN1 to MtN29, were thus identified in addition to clones for several known nodulins. All MtN genes were shown by Northern (RNA) hybridization analysis to be induced during nodulation, some of them well before nodule emergence. The MtN genes were classified into three groups depending on their expression kinetics. The expression of three MtN genes showed a limited induction by Nod factors purified from Rhizobium meliloti. Homologies with a variety of proteins were found for the deduced amino acid sequences of 10 of the MtN genes.</abstract><cop>St Paul, MN</cop><pub>APS Press</pub><pmid>8634476</pmid><doi>10.1094/MPMI-9-0233</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-5596-9420</orcidid><orcidid>https://orcid.org/0000-0001-8804-4849</orcidid></addata></record> |
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| ispartof | Molecular plant-microbe interactions, 1996, Vol.9 (4), p.233-242 |
| issn | 0894-0282 1943-7706 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | adn Agronomy. Soil science and plant productions Amino Acid Sequence analytical methods arn mensajero arn messager Base Sequence Biochemistry, Molecular Biology Biological and medical sciences Blotting, Northern - methods chemical composition Cloning, Molecular composicion quimica composition chimique dna DNA, Complementary Economic plant physiology expresion genica expression des genes formation de nodosites Fundamental and applied biological sciences. Psychology gene gene expression genes identificacion identification Life Sciences Medicago sativa - genetics medicago truncatula Membrane Proteins messenger rna Molecular Sequence Data Nitrogen Fixation nodosite racinaire nodulacion nucleotide sequence nudosidades radiculares Plant Proteins - genetics Plant Roots - growth & development proteinas proteine proteins root nodulation root nodules secuencia nucleotidica sequence nucleotidique Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...) technique analytique tecnicas analiticas |
| title | Use of a subtractive hybridization approach to identify new Medicago truncatula genes induced during root nodule development |
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