Genetically engineered enteropathogenic Escherichia coli strain elicits a specific immune response and protects against a virulent challenge
Enteropathogenic Escherichia coli (EPEC), a major cause of severe disease with diarrhea in infants, is also involved in weaned rabbit colibacillosis. EPEC O103 is frequent in rabbit-fattening units of Western Europe. It causes high mortality and growth retardation, leading to substantial economic lo...
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| Veröffentlicht in: | Microbes and infection 2003-08, Vol.5 (10), p.857-867 |
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| creator | Boullier, Séverine Nougayrède, Jean-Philippe Marchès, Olivier Tasca, Christian Boury, Michèle Oswald, Eric De Rycke, Jean Milon, Alain |
| description | Enteropathogenic
Escherichia coli (EPEC), a major cause of severe disease with diarrhea in infants, is also involved in weaned rabbit colibacillosis. EPEC O103 is frequent in rabbit-fattening units of Western Europe. It causes high mortality and growth retardation, leading to substantial economic losses. We report here the construction by allelic exchange of an EPEC O103 strain mutated in
espB and
tir, two essential virulence genes. Upon live oral administration to weaned rabbits, the E22ΔTir/EspB mutant strain efficiently colonized the intestinal tract without any adverse consequences. The rabbits were challenged with the highly pathogenic parental strain E22. The mutant provided complete protection to rabbits and total resistance to intestinal colonization by E22. In addition, E22ΔTir/EspB strain induced a specific humoral response against the bacterial adhesin AF/R2. These Abs prevent bacterial attachment to epithelial cells in vitro. These results open the way for the development of an efficient vaccine strategy against rabbit EPEC infections. |
| doi_str_mv | 10.1016/S1286-4579(03)00175-8 |
| format | Article |
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Escherichia coli (EPEC), a major cause of severe disease with diarrhea in infants, is also involved in weaned rabbit colibacillosis. EPEC O103 is frequent in rabbit-fattening units of Western Europe. It causes high mortality and growth retardation, leading to substantial economic losses. We report here the construction by allelic exchange of an EPEC O103 strain mutated in
espB and
tir, two essential virulence genes. Upon live oral administration to weaned rabbits, the E22ΔTir/EspB mutant strain efficiently colonized the intestinal tract without any adverse consequences. The rabbits were challenged with the highly pathogenic parental strain E22. The mutant provided complete protection to rabbits and total resistance to intestinal colonization by E22. In addition, E22ΔTir/EspB strain induced a specific humoral response against the bacterial adhesin AF/R2. These Abs prevent bacterial attachment to epithelial cells in vitro. These results open the way for the development of an efficient vaccine strategy against rabbit EPEC infections.</description><identifier>ISSN: 1286-4579</identifier><identifier>EISSN: 1769-714X</identifier><identifier>DOI: 10.1016/S1286-4579(03)00175-8</identifier><identifier>PMID: 12919854</identifier><language>eng</language><publisher>Lausanne: Elsevier SAS</publisher><subject>Adhesins, Escherichia coli - immunology ; Administration, Oral ; Animals ; Anti-AF/R2 Abs ; Antibodies, Bacterial - blood ; Antigens, Bacterial - genetics ; Antigens, Bacterial - immunology ; Bacterial Adhesion - immunology ; Bacterial Outer Membrane Proteins - genetics ; Bacteriology ; Biological and medical sciences ; Body Weight ; Diarrhea - immunology ; Diarrhea - microbiology ; Enzyme-Linked Immunosorbent Assay ; EPEC O103 ; Escherichia coli - genetics ; Escherichia coli - growth & development ; Escherichia coli - immunology ; Escherichia coli - pathogenicity ; Escherichia coli Infections - immunology ; Escherichia coli Infections - microbiology ; Escherichia coli Proteins - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Deletion ; HeLa Cells ; Humans ; Intestines - microbiology ; Life Sciences ; Microbiology ; Mutagenesis, Insertional ; Rabbit ( Oryctolagus cuniculus) ; Rabbits ; Receptors, Cell Surface - genetics ; Vaccine ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Virulence - genetics</subject><ispartof>Microbes and infection, 2003-08, Vol.5 (10), p.857-867</ispartof><rights>2003</rights><rights>2003 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-ab617daf87b22ac89499041f74f8286c6e4f02d8502c06a779ae8d243d09e0303</citedby><cites>FETCH-LOGICAL-c456t-ab617daf87b22ac89499041f74f8286c6e4f02d8502c06a779ae8d243d09e0303</cites><orcidid>0000-0002-6802-1890</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S1286-4579(03)00175-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15027224$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12919854$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02682615$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Boullier, Séverine</creatorcontrib><creatorcontrib>Nougayrède, Jean-Philippe</creatorcontrib><creatorcontrib>Marchès, Olivier</creatorcontrib><creatorcontrib>Tasca, Christian</creatorcontrib><creatorcontrib>Boury, Michèle</creatorcontrib><creatorcontrib>Oswald, Eric</creatorcontrib><creatorcontrib>De Rycke, Jean</creatorcontrib><creatorcontrib>Milon, Alain</creatorcontrib><title>Genetically engineered enteropathogenic Escherichia coli strain elicits a specific immune response and protects against a virulent challenge</title><title>Microbes and infection</title><addtitle>Microbes Infect</addtitle><description>Enteropathogenic
Escherichia coli (EPEC), a major cause of severe disease with diarrhea in infants, is also involved in weaned rabbit colibacillosis. EPEC O103 is frequent in rabbit-fattening units of Western Europe. It causes high mortality and growth retardation, leading to substantial economic losses. We report here the construction by allelic exchange of an EPEC O103 strain mutated in
espB and
tir, two essential virulence genes. Upon live oral administration to weaned rabbits, the E22ΔTir/EspB mutant strain efficiently colonized the intestinal tract without any adverse consequences. The rabbits were challenged with the highly pathogenic parental strain E22. The mutant provided complete protection to rabbits and total resistance to intestinal colonization by E22. In addition, E22ΔTir/EspB strain induced a specific humoral response against the bacterial adhesin AF/R2. These Abs prevent bacterial attachment to epithelial cells in vitro. These results open the way for the development of an efficient vaccine strategy against rabbit EPEC infections.</description><subject>Adhesins, Escherichia coli - immunology</subject><subject>Administration, Oral</subject><subject>Animals</subject><subject>Anti-AF/R2 Abs</subject><subject>Antibodies, Bacterial - blood</subject><subject>Antigens, Bacterial - genetics</subject><subject>Antigens, Bacterial - immunology</subject><subject>Bacterial Adhesion - immunology</subject><subject>Bacterial Outer Membrane Proteins - genetics</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Body Weight</subject><subject>Diarrhea - immunology</subject><subject>Diarrhea - microbiology</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>EPEC O103</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - growth & development</subject><subject>Escherichia coli - immunology</subject><subject>Escherichia coli - pathogenicity</subject><subject>Escherichia coli Infections - immunology</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Escherichia coli Proteins - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Deletion</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Intestines - microbiology</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Mutagenesis, Insertional</subject><subject>Rabbit ( Oryctolagus cuniculus)</subject><subject>Rabbits</subject><subject>Receptors, Cell Surface - genetics</subject><subject>Vaccine</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Virulence - genetics</subject><issn>1286-4579</issn><issn>1769-714X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuO1DAQRSMEYh7wCSBvQMwiYDuO7axGo9E8kFpiAUjsLLdT6S6UOI2dtDT_wEdTmW6YJSuXrFN169YtijeCfxRc6E9fhbS6VLVpPvDqgnNh6tI-K06F0U1phPrxnOq_yElxlvNPgmqj1cviRMhGNLZWp8XvO4gwYfB9_8AgbjACJGipnCCNOz9txw1EDOwmhy0kDFv0LIw9sjwlj5FBjwGnzDzLOwjYEYrDMEdgCfJujBmYjy3bpXGCsHAb6soT8XtMc086LGxJnbThVfGi832G18f3vPh-e_Pt-r5cfbn7fH21KoOq9VT6tRam9Z01ayl9sI1qGq5EZ1RnyXHQoDouW1tzGbj2xjQebCtV1fIGeMWr8-LiMJeE3S7h4NODGz26-6uVW_641FZqUe8Fse8PLDn4NUOe3IA5QN_7COOcnbCWV9YoAusDGNKYc4Lu32TB3RKZe4zMLXk4XrnHyJylvrdHgXk9QPvUdcyIgHdHwGfKqUs-BsxPHNk0Ui7c5YEDOt0eIbkcEGKAFhOd3rUj_meVP2ZhtSU</recordid><startdate>20030801</startdate><enddate>20030801</enddate><creator>Boullier, Séverine</creator><creator>Nougayrède, Jean-Philippe</creator><creator>Marchès, Olivier</creator><creator>Tasca, Christian</creator><creator>Boury, Michèle</creator><creator>Oswald, Eric</creator><creator>De Rycke, Jean</creator><creator>Milon, Alain</creator><general>Elsevier SAS</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-6802-1890</orcidid></search><sort><creationdate>20030801</creationdate><title>Genetically engineered enteropathogenic Escherichia coli strain elicits a specific immune response and protects against a virulent challenge</title><author>Boullier, Séverine ; Nougayrède, Jean-Philippe ; Marchès, Olivier ; Tasca, Christian ; Boury, Michèle ; Oswald, Eric ; De Rycke, Jean ; Milon, Alain</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-ab617daf87b22ac89499041f74f8286c6e4f02d8502c06a779ae8d243d09e0303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adhesins, Escherichia coli - immunology</topic><topic>Administration, Oral</topic><topic>Animals</topic><topic>Anti-AF/R2 Abs</topic><topic>Antibodies, Bacterial - blood</topic><topic>Antigens, Bacterial - genetics</topic><topic>Antigens, Bacterial - immunology</topic><topic>Bacterial Adhesion - immunology</topic><topic>Bacterial Outer Membrane Proteins - genetics</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Body Weight</topic><topic>Diarrhea - immunology</topic><topic>Diarrhea - microbiology</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>EPEC O103</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - growth & development</topic><topic>Escherichia coli - immunology</topic><topic>Escherichia coli - pathogenicity</topic><topic>Escherichia coli Infections - immunology</topic><topic>Escherichia coli Infections - microbiology</topic><topic>Escherichia coli Proteins - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Deletion</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Intestines - microbiology</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Mutagenesis, Insertional</topic><topic>Rabbit ( Oryctolagus cuniculus)</topic><topic>Rabbits</topic><topic>Receptors, Cell Surface - genetics</topic><topic>Vaccine</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Virulence - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boullier, Séverine</creatorcontrib><creatorcontrib>Nougayrède, Jean-Philippe</creatorcontrib><creatorcontrib>Marchès, Olivier</creatorcontrib><creatorcontrib>Tasca, Christian</creatorcontrib><creatorcontrib>Boury, Michèle</creatorcontrib><creatorcontrib>Oswald, Eric</creatorcontrib><creatorcontrib>De Rycke, Jean</creatorcontrib><creatorcontrib>Milon, Alain</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Microbes and infection</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boullier, Séverine</au><au>Nougayrède, Jean-Philippe</au><au>Marchès, Olivier</au><au>Tasca, Christian</au><au>Boury, Michèle</au><au>Oswald, Eric</au><au>De Rycke, Jean</au><au>Milon, Alain</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetically engineered enteropathogenic Escherichia coli strain elicits a specific immune response and protects against a virulent challenge</atitle><jtitle>Microbes and infection</jtitle><addtitle>Microbes Infect</addtitle><date>2003-08-01</date><risdate>2003</risdate><volume>5</volume><issue>10</issue><spage>857</spage><epage>867</epage><pages>857-867</pages><issn>1286-4579</issn><eissn>1769-714X</eissn><abstract>Enteropathogenic
Escherichia coli (EPEC), a major cause of severe disease with diarrhea in infants, is also involved in weaned rabbit colibacillosis. EPEC O103 is frequent in rabbit-fattening units of Western Europe. It causes high mortality and growth retardation, leading to substantial economic losses. We report here the construction by allelic exchange of an EPEC O103 strain mutated in
espB and
tir, two essential virulence genes. Upon live oral administration to weaned rabbits, the E22ΔTir/EspB mutant strain efficiently colonized the intestinal tract without any adverse consequences. The rabbits were challenged with the highly pathogenic parental strain E22. The mutant provided complete protection to rabbits and total resistance to intestinal colonization by E22. In addition, E22ΔTir/EspB strain induced a specific humoral response against the bacterial adhesin AF/R2. These Abs prevent bacterial attachment to epithelial cells in vitro. These results open the way for the development of an efficient vaccine strategy against rabbit EPEC infections.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>Paris</cop><pub>Elsevier SAS</pub><pmid>12919854</pmid><doi>10.1016/S1286-4579(03)00175-8</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-6802-1890</orcidid></addata></record> |
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| ispartof | Microbes and infection, 2003-08, Vol.5 (10), p.857-867 |
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| subjects | Adhesins, Escherichia coli - immunology Administration, Oral Animals Anti-AF/R2 Abs Antibodies, Bacterial - blood Antigens, Bacterial - genetics Antigens, Bacterial - immunology Bacterial Adhesion - immunology Bacterial Outer Membrane Proteins - genetics Bacteriology Biological and medical sciences Body Weight Diarrhea - immunology Diarrhea - microbiology Enzyme-Linked Immunosorbent Assay EPEC O103 Escherichia coli - genetics Escherichia coli - growth & development Escherichia coli - immunology Escherichia coli - pathogenicity Escherichia coli Infections - immunology Escherichia coli Infections - microbiology Escherichia coli Proteins - genetics Fundamental and applied biological sciences. Psychology Gene Deletion HeLa Cells Humans Intestines - microbiology Life Sciences Microbiology Mutagenesis, Insertional Rabbit ( Oryctolagus cuniculus) Rabbits Receptors, Cell Surface - genetics Vaccine Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Virulence - genetics |
| title | Genetically engineered enteropathogenic Escherichia coli strain elicits a specific immune response and protects against a virulent challenge |
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