Haemocyte changes in D. Melanogaster in response to long gland components of the parasitoid wasp Leptopilina boulardi: a Rho-GAP protein as an important factor
The hymenopteran wasp Leptopilina boulardi (Figitidae) is a larval solitary parasitoid of Drosophila larvae of the melanogaster sub-group. The factors used by parasitoid females to prevent encapsulation of their eggs by the host are localized in the female long gland and reservoir. We report here th...
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| Veröffentlicht in: | Journal of insect physiology 2005-02, Vol.51 (2), p.161-170 |
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| creator | Labrosse, C. Eslin, P. Doury, G. Drezen, J.M. Poirié, M. |
| description | The hymenopteran wasp
Leptopilina boulardi (Figitidae) is a larval solitary parasitoid of
Drosophila larvae of the
melanogaster sub-group. The factors used by parasitoid females to prevent encapsulation of their eggs by the host are localized in the female long gland and reservoir. We report here the physiological effects of these factors on host haemocytes using in vivo injection experiments. The total number of haemocytes, the number of plasmatocytes and the number of crystal cells were not modified by injection of long gland extracts. In contrast, long gland extracts either from virulent or avirulent strains had a significant effect on the lamellocyte number. Compared to the Ringer control, the avirulent long gland products induced an increase of the lamellocyte number while virulent extracts induced a drastic decrease together with an alteration of the morphology of these cells. Interestingly, changes in the lamellocyte morphology were also observed following injection of the P4 protein, a major component of
L. boulardi female long glands that displays a strong immune suppressive effect on
Drosophila larvae. The implication of the P4 protein in suppressing the host cellular immunity is discussed in correlation with its predicted molecular function as a Rho-GAP protein. |
| doi_str_mv | 10.1016/j.jinsphys.2004.10.004 |
| format | Article |
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Leptopilina boulardi (Figitidae) is a larval solitary parasitoid of
Drosophila larvae of the
melanogaster sub-group. The factors used by parasitoid females to prevent encapsulation of their eggs by the host are localized in the female long gland and reservoir. We report here the physiological effects of these factors on host haemocytes using in vivo injection experiments. The total number of haemocytes, the number of plasmatocytes and the number of crystal cells were not modified by injection of long gland extracts. In contrast, long gland extracts either from virulent or avirulent strains had a significant effect on the lamellocyte number. Compared to the Ringer control, the avirulent long gland products induced an increase of the lamellocyte number while virulent extracts induced a drastic decrease together with an alteration of the morphology of these cells. Interestingly, changes in the lamellocyte morphology were also observed following injection of the P4 protein, a major component of
L. boulardi female long glands that displays a strong immune suppressive effect on
Drosophila larvae. The implication of the P4 protein in suppressing the host cellular immunity is discussed in correlation with its predicted molecular function as a Rho-GAP protein.</description><identifier>ISSN: 0022-1910</identifier><identifier>EISSN: 1879-1611</identifier><identifier>DOI: 10.1016/j.jinsphys.2004.10.004</identifier><identifier>PMID: 15749101</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Analysis of Variance ; animal glands ; animal tissue extracts ; Animals ; Blood Cell Count ; cell morphology ; cell-mediated immunity ; Drosophila ; Drosophila melanogaster ; Drosophila melanogaster - drug effects ; Drosophila melanogaster - parasitology ; Encapsulation ; Exocrine Glands - metabolism ; Female ; Figitidae ; GTPase-Activating Proteins - metabolism ; GTPase-Activating Proteins - pharmacology ; Haemocytes ; hemocytes ; Hemocytes - drug effects ; Hemocytes - metabolism ; host immunosuppression ; host-parasite relationships ; immune evasion ; Immune Tolerance - drug effects ; Immunity, Cellular - drug effects ; immunosuppression (physiological) ; insect immunity ; insect proteins ; lamellocytes ; Larva - drug effects ; Larva - parasitology ; Leptopilina boulardi ; Life Sciences ; P4 protein ; Parasitoid wasps ; parasitoids ; Rho-GAP protein ; Tissue Extracts - metabolism ; Tissue Extracts - pharmacology ; virulence ; Wasps - metabolism</subject><ispartof>Journal of insect physiology, 2005-02, Vol.51 (2), p.161-170</ispartof><rights>2004 Elsevier Ltd</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c521t-6231c2720e450f4a2cf502296ff513dd255cfbeffb7b85ff37b861b03a66bfc73</citedby><cites>FETCH-LOGICAL-c521t-6231c2720e450f4a2cf502296ff513dd255cfbeffb7b85ff37b861b03a66bfc73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jinsphys.2004.10.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15749101$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02682287$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Labrosse, C.</creatorcontrib><creatorcontrib>Eslin, P.</creatorcontrib><creatorcontrib>Doury, G.</creatorcontrib><creatorcontrib>Drezen, J.M.</creatorcontrib><creatorcontrib>Poirié, M.</creatorcontrib><title>Haemocyte changes in D. Melanogaster in response to long gland components of the parasitoid wasp Leptopilina boulardi: a Rho-GAP protein as an important factor</title><title>Journal of insect physiology</title><addtitle>J Insect Physiol</addtitle><description>The hymenopteran wasp
Leptopilina boulardi (Figitidae) is a larval solitary parasitoid of
Drosophila larvae of the
melanogaster sub-group. The factors used by parasitoid females to prevent encapsulation of their eggs by the host are localized in the female long gland and reservoir. We report here the physiological effects of these factors on host haemocytes using in vivo injection experiments. The total number of haemocytes, the number of plasmatocytes and the number of crystal cells were not modified by injection of long gland extracts. In contrast, long gland extracts either from virulent or avirulent strains had a significant effect on the lamellocyte number. Compared to the Ringer control, the avirulent long gland products induced an increase of the lamellocyte number while virulent extracts induced a drastic decrease together with an alteration of the morphology of these cells. Interestingly, changes in the lamellocyte morphology were also observed following injection of the P4 protein, a major component of
L. boulardi female long glands that displays a strong immune suppressive effect on
Drosophila larvae. The implication of the P4 protein in suppressing the host cellular immunity is discussed in correlation with its predicted molecular function as a Rho-GAP protein.</description><subject>Analysis of Variance</subject><subject>animal glands</subject><subject>animal tissue extracts</subject><subject>Animals</subject><subject>Blood Cell Count</subject><subject>cell morphology</subject><subject>cell-mediated immunity</subject><subject>Drosophila</subject><subject>Drosophila melanogaster</subject><subject>Drosophila melanogaster - drug effects</subject><subject>Drosophila melanogaster - parasitology</subject><subject>Encapsulation</subject><subject>Exocrine Glands - metabolism</subject><subject>Female</subject><subject>Figitidae</subject><subject>GTPase-Activating Proteins - metabolism</subject><subject>GTPase-Activating Proteins - pharmacology</subject><subject>Haemocytes</subject><subject>hemocytes</subject><subject>Hemocytes - drug effects</subject><subject>Hemocytes - metabolism</subject><subject>host immunosuppression</subject><subject>host-parasite relationships</subject><subject>immune evasion</subject><subject>Immune Tolerance - drug effects</subject><subject>Immunity, Cellular - drug effects</subject><subject>immunosuppression (physiological)</subject><subject>insect immunity</subject><subject>insect proteins</subject><subject>lamellocytes</subject><subject>Larva - drug effects</subject><subject>Larva - parasitology</subject><subject>Leptopilina boulardi</subject><subject>Life Sciences</subject><subject>P4 protein</subject><subject>Parasitoid wasps</subject><subject>parasitoids</subject><subject>Rho-GAP protein</subject><subject>Tissue Extracts - metabolism</subject><subject>Tissue Extracts - pharmacology</subject><subject>virulence</subject><subject>Wasps - metabolism</subject><issn>0022-1910</issn><issn>1879-1611</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2O0zAUhSMEYjoDrzB4hcQiwXZqJ2VFNQxTpCIQMGvrxrluXaV2sN1BfRpeFUctsJzVkY6_--N7iuKa0YpRJt_uqp11cdweY8UpnWezyvKkmLG2WZRMMva0mFHKeckWjF4UlzHuKKVCtuJ5ccFEM882mxW_V4B7r48Jid6C22Ak1pEPFfmMAzi_gZgwTFbAOHoXkSRPBu82ZJPfe6L9PtvoUiTekLRFMkKAaJO3PfkFcSRrHJMf7WAdkM4fBgi9fUeAfNv68m75lYzBJ8wDIBJwxOZ2IYFLxIBOPrwonhkYIr4861Vx__H2x82qXH-5-3SzXJdacJZKyWumecMpzgU1c-DaiPz5hTRGsLrvuRDadGhM13StMKbOIllHa5CyM7qpr4o3p75bGNQY7B7CUXmwarVcq8mjXLact80Dy-zrE5tX_3nAmNTeRo1DPgj6Q1SymbeCLx4HWdPWTdvUGZQnUAcfY0DzbwVG1ZS32qm_easp78nPkguvzxMO3R77_2XngDPw6gQY8Ao2wUZ1_53nB0oXbaZkJt6fCMznfbAYVNQWncbeBtRJ9d4-tsUfNijKjg</recordid><startdate>20050201</startdate><enddate>20050201</enddate><creator>Labrosse, C.</creator><creator>Eslin, P.</creator><creator>Doury, G.</creator><creator>Drezen, J.M.</creator><creator>Poirié, M.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>20050201</creationdate><title>Haemocyte changes in D. Melanogaster in response to long gland components of the parasitoid wasp Leptopilina boulardi: a Rho-GAP protein as an important factor</title><author>Labrosse, C. ; Eslin, P. ; Doury, G. ; Drezen, J.M. ; Poirié, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c521t-6231c2720e450f4a2cf502296ff513dd255cfbeffb7b85ff37b861b03a66bfc73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Analysis of Variance</topic><topic>animal glands</topic><topic>animal tissue extracts</topic><topic>Animals</topic><topic>Blood Cell Count</topic><topic>cell morphology</topic><topic>cell-mediated immunity</topic><topic>Drosophila</topic><topic>Drosophila melanogaster</topic><topic>Drosophila melanogaster - drug effects</topic><topic>Drosophila melanogaster - parasitology</topic><topic>Encapsulation</topic><topic>Exocrine Glands - metabolism</topic><topic>Female</topic><topic>Figitidae</topic><topic>GTPase-Activating Proteins - metabolism</topic><topic>GTPase-Activating Proteins - pharmacology</topic><topic>Haemocytes</topic><topic>hemocytes</topic><topic>Hemocytes - drug effects</topic><topic>Hemocytes - metabolism</topic><topic>host immunosuppression</topic><topic>host-parasite relationships</topic><topic>immune evasion</topic><topic>Immune Tolerance - drug effects</topic><topic>Immunity, Cellular - drug effects</topic><topic>immunosuppression (physiological)</topic><topic>insect immunity</topic><topic>insect proteins</topic><topic>lamellocytes</topic><topic>Larva - drug effects</topic><topic>Larva - parasitology</topic><topic>Leptopilina boulardi</topic><topic>Life Sciences</topic><topic>P4 protein</topic><topic>Parasitoid wasps</topic><topic>parasitoids</topic><topic>Rho-GAP protein</topic><topic>Tissue Extracts - metabolism</topic><topic>Tissue Extracts - pharmacology</topic><topic>virulence</topic><topic>Wasps - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Labrosse, C.</creatorcontrib><creatorcontrib>Eslin, P.</creatorcontrib><creatorcontrib>Doury, G.</creatorcontrib><creatorcontrib>Drezen, J.M.</creatorcontrib><creatorcontrib>Poirié, M.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of insect physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Labrosse, C.</au><au>Eslin, P.</au><au>Doury, G.</au><au>Drezen, J.M.</au><au>Poirié, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Haemocyte changes in D. Melanogaster in response to long gland components of the parasitoid wasp Leptopilina boulardi: a Rho-GAP protein as an important factor</atitle><jtitle>Journal of insect physiology</jtitle><addtitle>J Insect Physiol</addtitle><date>2005-02-01</date><risdate>2005</risdate><volume>51</volume><issue>2</issue><spage>161</spage><epage>170</epage><pages>161-170</pages><issn>0022-1910</issn><eissn>1879-1611</eissn><abstract>The hymenopteran wasp
Leptopilina boulardi (Figitidae) is a larval solitary parasitoid of
Drosophila larvae of the
melanogaster sub-group. The factors used by parasitoid females to prevent encapsulation of their eggs by the host are localized in the female long gland and reservoir. We report here the physiological effects of these factors on host haemocytes using in vivo injection experiments. The total number of haemocytes, the number of plasmatocytes and the number of crystal cells were not modified by injection of long gland extracts. In contrast, long gland extracts either from virulent or avirulent strains had a significant effect on the lamellocyte number. Compared to the Ringer control, the avirulent long gland products induced an increase of the lamellocyte number while virulent extracts induced a drastic decrease together with an alteration of the morphology of these cells. Interestingly, changes in the lamellocyte morphology were also observed following injection of the P4 protein, a major component of
L. boulardi female long glands that displays a strong immune suppressive effect on
Drosophila larvae. The implication of the P4 protein in suppressing the host cellular immunity is discussed in correlation with its predicted molecular function as a Rho-GAP protein.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>15749101</pmid><doi>10.1016/j.jinsphys.2004.10.004</doi><tpages>10</tpages></addata></record> |
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| ispartof | Journal of insect physiology, 2005-02, Vol.51 (2), p.161-170 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Analysis of Variance animal glands animal tissue extracts Animals Blood Cell Count cell morphology cell-mediated immunity Drosophila Drosophila melanogaster Drosophila melanogaster - drug effects Drosophila melanogaster - parasitology Encapsulation Exocrine Glands - metabolism Female Figitidae GTPase-Activating Proteins - metabolism GTPase-Activating Proteins - pharmacology Haemocytes hemocytes Hemocytes - drug effects Hemocytes - metabolism host immunosuppression host-parasite relationships immune evasion Immune Tolerance - drug effects Immunity, Cellular - drug effects immunosuppression (physiological) insect immunity insect proteins lamellocytes Larva - drug effects Larva - parasitology Leptopilina boulardi Life Sciences P4 protein Parasitoid wasps parasitoids Rho-GAP protein Tissue Extracts - metabolism Tissue Extracts - pharmacology virulence Wasps - metabolism |
| title | Haemocyte changes in D. Melanogaster in response to long gland components of the parasitoid wasp Leptopilina boulardi: a Rho-GAP protein as an important factor |
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