The ethanol switch: a tool for tissue‐specific gene induction during plant development
Summary Controlled gene expression in time and space is a powerful tool for the analysis of gene function during plant development. Here, we report ethanol inducible gene expression in defined sub‐domains of the shoot apical and floral meristems. For this, expression of an ethanol‐regulated transcri...
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| Veröffentlicht in: | The Plant journal : for cell and molecular biology 2003-12, Vol.36 (6), p.918-930 |
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| container_title | The Plant journal : for cell and molecular biology |
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| creator | Deveaux, Yves Peaucelle, Alexis Roberts, Gethin R. Coen, Enrico Simon, Rudiger Mizukami, Yukiko Traas, Jan Murray, James A. H. Doonan, John H. Laufs, Patrick |
| description | Summary
Controlled gene expression in time and space is a powerful tool for the analysis of gene function during plant development. Here, we report ethanol inducible gene expression in defined sub‐domains of the shoot apical and floral meristems. For this, expression of an ethanol‐regulated transcription factor, ALCR, is restricted to precise domains using specific promoters. Gene expression activation is followed using reporters under the control of the alcA promoter, which responds to ALCR only in the presence of the ethanol. We demonstrate that precise control of spatially limited gene expression can be achieved. The kinetics of reporter gene activation and inactivation following a pulse of ethanol induction shows that the system is dynamic and suitable for precise temporal control of expression. The system is both flexible and robust, permitting simultaneous expression of two genes in a given domain or, conversely, the expression of a gene in two separate domains. We also show that this strategy can be applied to mis‐express genes with developmental roles, by manipulating expression of the SHOOT MERISTEMLESS (STM) and CYCLIN D3;1 (CYCD3;1) genes during plant development. |
| doi_str_mv | 10.1046/j.1365-313X.2003.01922.x |
| format | Article |
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Controlled gene expression in time and space is a powerful tool for the analysis of gene function during plant development. Here, we report ethanol inducible gene expression in defined sub‐domains of the shoot apical and floral meristems. For this, expression of an ethanol‐regulated transcription factor, ALCR, is restricted to precise domains using specific promoters. Gene expression activation is followed using reporters under the control of the alcA promoter, which responds to ALCR only in the presence of the ethanol. We demonstrate that precise control of spatially limited gene expression can be achieved. The kinetics of reporter gene activation and inactivation following a pulse of ethanol induction shows that the system is dynamic and suitable for precise temporal control of expression. The system is both flexible and robust, permitting simultaneous expression of two genes in a given domain or, conversely, the expression of a gene in two separate domains. We also show that this strategy can be applied to mis‐express genes with developmental roles, by manipulating expression of the SHOOT MERISTEMLESS (STM) and CYCLIN D3;1 (CYCD3;1) genes during plant development.</description><identifier>ISSN: 0960-7412</identifier><identifier>EISSN: 1365-313X</identifier><identifier>DOI: 10.1046/j.1365-313X.2003.01922.x</identifier><identifier>PMID: 14675455</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>1 gene ; ALCR protein ; Arabidopsis ; Arabidopsis - drug effects ; Arabidopsis - genetics ; Arabidopsis - growth & development ; Base Sequence ; Biological and medical sciences ; conditional gene expression ; CYCD3 ; CYCLIN D ; DNA Primers ; DNA, Plant - genetics ; Ethanol - pharmacology ; ethanol switch ; Flowers - drug effects ; Flowers - genetics ; Flowers - growth & development ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Developmental - drug effects ; Gene Expression Regulation, Plant - drug effects ; Genetic Techniques ; Life Sciences ; meristem ; Plant growth. Development of the storage organs ; Plant physiology and development ; Promoter Regions, Genetic - genetics ; SHOOT MERISTEMLESS ; SHOOT MERISTEMLESS gene ; Transcriptional Activation ; Transformation, Genetic ; Vegetative apparatus, growth and morphogenesis. Senescence</subject><ispartof>The Plant journal : for cell and molecular biology, 2003-12, Vol.36 (6), p.918-930</ispartof><rights>2004 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5762-e0cd0d1b9813a73a431fe4f6e110baa95a05bc1622e523b1ede6b9c0637f4d683</citedby><cites>FETCH-LOGICAL-c5762-e0cd0d1b9813a73a431fe4f6e110baa95a05bc1622e523b1ede6b9c0637f4d683</cites><orcidid>0000-0002-3297-1317 ; 0000-0003-4459-3445 ; 0000-0002-8582-6500</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-313X.2003.01922.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-313X.2003.01922.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,315,781,785,886,1418,1434,27929,27930,45579,45580,46414,46838</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15338610$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14675455$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02680631$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Deveaux, Yves</creatorcontrib><creatorcontrib>Peaucelle, Alexis</creatorcontrib><creatorcontrib>Roberts, Gethin R.</creatorcontrib><creatorcontrib>Coen, Enrico</creatorcontrib><creatorcontrib>Simon, Rudiger</creatorcontrib><creatorcontrib>Mizukami, Yukiko</creatorcontrib><creatorcontrib>Traas, Jan</creatorcontrib><creatorcontrib>Murray, James A. H.</creatorcontrib><creatorcontrib>Doonan, John H.</creatorcontrib><creatorcontrib>Laufs, Patrick</creatorcontrib><title>The ethanol switch: a tool for tissue‐specific gene induction during plant development</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>Summary
Controlled gene expression in time and space is a powerful tool for the analysis of gene function during plant development. Here, we report ethanol inducible gene expression in defined sub‐domains of the shoot apical and floral meristems. For this, expression of an ethanol‐regulated transcription factor, ALCR, is restricted to precise domains using specific promoters. Gene expression activation is followed using reporters under the control of the alcA promoter, which responds to ALCR only in the presence of the ethanol. We demonstrate that precise control of spatially limited gene expression can be achieved. The kinetics of reporter gene activation and inactivation following a pulse of ethanol induction shows that the system is dynamic and suitable for precise temporal control of expression. The system is both flexible and robust, permitting simultaneous expression of two genes in a given domain or, conversely, the expression of a gene in two separate domains. We also show that this strategy can be applied to mis‐express genes with developmental roles, by manipulating expression of the SHOOT MERISTEMLESS (STM) and CYCLIN D3;1 (CYCD3;1) genes during plant development.</description><subject>1 gene</subject><subject>ALCR protein</subject><subject>Arabidopsis</subject><subject>Arabidopsis - drug effects</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - growth & development</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>conditional gene expression</subject><subject>CYCD3</subject><subject>CYCLIN D</subject><subject>DNA Primers</subject><subject>DNA, Plant - genetics</subject><subject>Ethanol - pharmacology</subject><subject>ethanol switch</subject><subject>Flowers - drug effects</subject><subject>Flowers - genetics</subject><subject>Flowers - growth & development</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Developmental - drug effects</subject><subject>Gene Expression Regulation, Plant - drug effects</subject><subject>Genetic Techniques</subject><subject>Life Sciences</subject><subject>meristem</subject><subject>Plant growth. Development of the storage organs</subject><subject>Plant physiology and development</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>SHOOT MERISTEMLESS</subject><subject>SHOOT MERISTEMLESS gene</subject><subject>Transcriptional Activation</subject><subject>Transformation, Genetic</subject><subject>Vegetative apparatus, growth and morphogenesis. Senescence</subject><issn>0960-7412</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAQxy0EokvhFZAvIHFI8PgrDhKHqgIKWgkOi9Sb5TiTrlfZOMRJP248As_Ik5Cwq_YIJ489vxn_pR8hFFgOTOq3uxyEVpkAcZlzxkTOoOQ8v31EVveNx2TFSs2yQgI_Ic9S2jEGhdDyKTkBqQsllVqRy80WKY5b18WWppsw-u076ugY52sTBzqGlCb8_fNX6tGHJnh6hR3S0NWTH0PsaD0Nobuifeu6kdZ4jW3s99iNz8mTxrUJXxzPU_L944fN-UW2_vrp8_nZOvOq0DxD5mtWQ1UaEK4QTgpoUDYaAVjlXKkcU5UHzTkqLirAGnVVeqZF0chaG3FK3hz2bl1r-yHs3XBnowv24mxtlzfGtZlxuIaZfX1g-yH-mDCNdh-Sx3bOjnFKtgBZcODmnyAUxhhhxAyaA-iHmNKAzX0EYHZRZXd2MWIXI3ZRZf-qsrfz6MvjH1O1x_ph8OhmBl4dAZe8a5vBdT6kB04JYTSwmXt_4G5Ci3f_HcBuvn1ZKvEHfQqv5Q</recordid><startdate>200312</startdate><enddate>200312</enddate><creator>Deveaux, Yves</creator><creator>Peaucelle, Alexis</creator><creator>Roberts, Gethin R.</creator><creator>Coen, Enrico</creator><creator>Simon, Rudiger</creator><creator>Mizukami, Yukiko</creator><creator>Traas, Jan</creator><creator>Murray, James A. H.</creator><creator>Doonan, John H.</creator><creator>Laufs, Patrick</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><general>Wiley</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-3297-1317</orcidid><orcidid>https://orcid.org/0000-0003-4459-3445</orcidid><orcidid>https://orcid.org/0000-0002-8582-6500</orcidid></search><sort><creationdate>200312</creationdate><title>The ethanol switch: a tool for tissue‐specific gene induction during plant development</title><author>Deveaux, Yves ; Peaucelle, Alexis ; Roberts, Gethin R. ; Coen, Enrico ; Simon, Rudiger ; Mizukami, Yukiko ; Traas, Jan ; Murray, James A. H. ; Doonan, John H. ; Laufs, Patrick</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5762-e0cd0d1b9813a73a431fe4f6e110baa95a05bc1622e523b1ede6b9c0637f4d683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>1 gene</topic><topic>ALCR protein</topic><topic>Arabidopsis</topic><topic>Arabidopsis - drug effects</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - growth & development</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>conditional gene expression</topic><topic>CYCD3</topic><topic>CYCLIN D</topic><topic>DNA Primers</topic><topic>DNA, Plant - genetics</topic><topic>Ethanol - pharmacology</topic><topic>ethanol switch</topic><topic>Flowers - drug effects</topic><topic>Flowers - genetics</topic><topic>Flowers - growth & development</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Developmental - drug effects</topic><topic>Gene Expression Regulation, Plant - drug effects</topic><topic>Genetic Techniques</topic><topic>Life Sciences</topic><topic>meristem</topic><topic>Plant growth. Development of the storage organs</topic><topic>Plant physiology and development</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>SHOOT MERISTEMLESS</topic><topic>SHOOT MERISTEMLESS gene</topic><topic>Transcriptional Activation</topic><topic>Transformation, Genetic</topic><topic>Vegetative apparatus, growth and morphogenesis. Senescence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deveaux, Yves</creatorcontrib><creatorcontrib>Peaucelle, Alexis</creatorcontrib><creatorcontrib>Roberts, Gethin R.</creatorcontrib><creatorcontrib>Coen, Enrico</creatorcontrib><creatorcontrib>Simon, Rudiger</creatorcontrib><creatorcontrib>Mizukami, Yukiko</creatorcontrib><creatorcontrib>Traas, Jan</creatorcontrib><creatorcontrib>Murray, James A. H.</creatorcontrib><creatorcontrib>Doonan, John H.</creatorcontrib><creatorcontrib>Laufs, Patrick</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>The Plant journal : for cell and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deveaux, Yves</au><au>Peaucelle, Alexis</au><au>Roberts, Gethin R.</au><au>Coen, Enrico</au><au>Simon, Rudiger</au><au>Mizukami, Yukiko</au><au>Traas, Jan</au><au>Murray, James A. H.</au><au>Doonan, John H.</au><au>Laufs, Patrick</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The ethanol switch: a tool for tissue‐specific gene induction during plant development</atitle><jtitle>The Plant journal : for cell and molecular biology</jtitle><addtitle>Plant J</addtitle><date>2003-12</date><risdate>2003</risdate><volume>36</volume><issue>6</issue><spage>918</spage><epage>930</epage><pages>918-930</pages><issn>0960-7412</issn><eissn>1365-313X</eissn><abstract>Summary
Controlled gene expression in time and space is a powerful tool for the analysis of gene function during plant development. Here, we report ethanol inducible gene expression in defined sub‐domains of the shoot apical and floral meristems. For this, expression of an ethanol‐regulated transcription factor, ALCR, is restricted to precise domains using specific promoters. Gene expression activation is followed using reporters under the control of the alcA promoter, which responds to ALCR only in the presence of the ethanol. We demonstrate that precise control of spatially limited gene expression can be achieved. The kinetics of reporter gene activation and inactivation following a pulse of ethanol induction shows that the system is dynamic and suitable for precise temporal control of expression. The system is both flexible and robust, permitting simultaneous expression of two genes in a given domain or, conversely, the expression of a gene in two separate domains. We also show that this strategy can be applied to mis‐express genes with developmental roles, by manipulating expression of the SHOOT MERISTEMLESS (STM) and CYCLIN D3;1 (CYCD3;1) genes during plant development.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>14675455</pmid><doi>10.1046/j.1365-313X.2003.01922.x</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-3297-1317</orcidid><orcidid>https://orcid.org/0000-0003-4459-3445</orcidid><orcidid>https://orcid.org/0000-0002-8582-6500</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 1 gene ALCR protein Arabidopsis Arabidopsis - drug effects Arabidopsis - genetics Arabidopsis - growth & development Base Sequence Biological and medical sciences conditional gene expression CYCD3 CYCLIN D DNA Primers DNA, Plant - genetics Ethanol - pharmacology ethanol switch Flowers - drug effects Flowers - genetics Flowers - growth & development Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Developmental - drug effects Gene Expression Regulation, Plant - drug effects Genetic Techniques Life Sciences meristem Plant growth. Development of the storage organs Plant physiology and development Promoter Regions, Genetic - genetics SHOOT MERISTEMLESS SHOOT MERISTEMLESS gene Transcriptional Activation Transformation, Genetic Vegetative apparatus, growth and morphogenesis. Senescence |
| title | The ethanol switch: a tool for tissue‐specific gene induction during plant development |
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