Constitutive expression of Vr-ERE gene in transformed grapevines confers enhanced resistance to eutypine, a toxin from Eutypa lata
Eutypine (4-hydroxy-3-[3-methyl-3-butene-1-ynyl] benzaldehyde) is a toxin produced by Eutypa lata, the causal agent of eutypa dieback of grapevines. Recently, a eutypine detoxifying gene ( Vr-ERE) encoding an NADPH-dependent aldehyde reductase which converts eutypine into the corresponding alcohol,...
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| Veröffentlicht in: | Plant science (Limerick) 2003-05, Vol.164 (5), p.809-814 |
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| creator | Legrand, Valérie Dalmayrac, Sylvie Latché, Alain Pech, Jean-Claude Bouzayen, Mondher Fallot, Jean Torregrosa, Laurent Bouquet, Alain Roustan, Jean-Paul |
| description | Eutypine (4-hydroxy-3-[3-methyl-3-butene-1-ynyl] benzaldehyde) is a toxin produced by
Eutypa lata, the causal agent of eutypa dieback of grapevines. Recently, a eutypine detoxifying gene (
Vr-ERE) encoding an NADPH-dependent aldehyde reductase which converts eutypine into the corresponding alcohol, eutypinol, a non-toxic form of the toxin, has been cloned. We report here the obtention of transgenic plants from grapevine rootstock 110 Richter
Vitis berlandieri×
V. rupestris that express the
Vr-ERE gene. The over-expression of
Vr-ERE gene in grapevine tissues increases their detoxification capacity. The growth and development of the transgenic plants cultured in vitro exhibiting a high Vr-ERE activity were not affected by the presence of 300 μM of toxin whereas the growth of untransformed control plants was highly inhibited. These results suggest that the
Vr-ERE gene is an efficient candidate to confer resistance to the toxin and opens new opportunities to study the role of the toxin in the development of the disease. |
| doi_str_mv | 10.1016/S0168-9452(03)00069-4 |
| format | Article |
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Eutypa lata, the causal agent of eutypa dieback of grapevines. Recently, a eutypine detoxifying gene (
Vr-ERE) encoding an NADPH-dependent aldehyde reductase which converts eutypine into the corresponding alcohol, eutypinol, a non-toxic form of the toxin, has been cloned. We report here the obtention of transgenic plants from grapevine rootstock 110 Richter
Vitis berlandieri×
V. rupestris that express the
Vr-ERE gene. The over-expression of
Vr-ERE gene in grapevine tissues increases their detoxification capacity. The growth and development of the transgenic plants cultured in vitro exhibiting a high Vr-ERE activity were not affected by the presence of 300 μM of toxin whereas the growth of untransformed control plants was highly inhibited. These results suggest that the
Vr-ERE gene is an efficient candidate to confer resistance to the toxin and opens new opportunities to study the role of the toxin in the development of the disease.</description><identifier>ISSN: 0168-9452</identifier><identifier>EISSN: 1873-2259</identifier><identifier>DOI: 10.1016/S0168-9452(03)00069-4</identifier><identifier>CODEN: PLSCE4</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Agronomy. Soil science and plant productions ; Biological and medical sciences ; Detoxification ; Eutypa dieback ; Eutypa lata ; Eutypine ; Fundamental and applied biological sciences. Psychology ; Genetic transformation ; Genetics ; Genetics and breeding of economic plants ; Grapevine ; Life Sciences ; Pest resistance ; Plant pathogens ; Plants genetics ; Reductase ; Toxin ; Varietal selection. Specialized plant breeding, plant breeding aims</subject><ispartof>Plant science (Limerick), 2003-05, Vol.164 (5), p.809-814</ispartof><rights>2003 Elsevier Science Ireland Ltd</rights><rights>2003 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-7a8bca8f14b2dd3c337657bbadfe224d981f93d3b3edaad648cb740d56a772273</citedby><cites>FETCH-LOGICAL-c402t-7a8bca8f14b2dd3c337657bbadfe224d981f93d3b3edaad648cb740d56a772273</cites><orcidid>0000-0003-1622-5610</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0168945203000694$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14715090$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02679449$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Legrand, Valérie</creatorcontrib><creatorcontrib>Dalmayrac, Sylvie</creatorcontrib><creatorcontrib>Latché, Alain</creatorcontrib><creatorcontrib>Pech, Jean-Claude</creatorcontrib><creatorcontrib>Bouzayen, Mondher</creatorcontrib><creatorcontrib>Fallot, Jean</creatorcontrib><creatorcontrib>Torregrosa, Laurent</creatorcontrib><creatorcontrib>Bouquet, Alain</creatorcontrib><creatorcontrib>Roustan, Jean-Paul</creatorcontrib><title>Constitutive expression of Vr-ERE gene in transformed grapevines confers enhanced resistance to eutypine, a toxin from Eutypa lata</title><title>Plant science (Limerick)</title><description>Eutypine (4-hydroxy-3-[3-methyl-3-butene-1-ynyl] benzaldehyde) is a toxin produced by
Eutypa lata, the causal agent of eutypa dieback of grapevines. Recently, a eutypine detoxifying gene (
Vr-ERE) encoding an NADPH-dependent aldehyde reductase which converts eutypine into the corresponding alcohol, eutypinol, a non-toxic form of the toxin, has been cloned. We report here the obtention of transgenic plants from grapevine rootstock 110 Richter
Vitis berlandieri×
V. rupestris that express the
Vr-ERE gene. The over-expression of
Vr-ERE gene in grapevine tissues increases their detoxification capacity. The growth and development of the transgenic plants cultured in vitro exhibiting a high Vr-ERE activity were not affected by the presence of 300 μM of toxin whereas the growth of untransformed control plants was highly inhibited. These results suggest that the
Vr-ERE gene is an efficient candidate to confer resistance to the toxin and opens new opportunities to study the role of the toxin in the development of the disease.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>Detoxification</subject><subject>Eutypa dieback</subject><subject>Eutypa lata</subject><subject>Eutypine</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic transformation</subject><subject>Genetics</subject><subject>Genetics and breeding of economic plants</subject><subject>Grapevine</subject><subject>Life Sciences</subject><subject>Pest resistance</subject><subject>Plant pathogens</subject><subject>Plants genetics</subject><subject>Reductase</subject><subject>Toxin</subject><subject>Varietal selection. Specialized plant breeding, plant breeding aims</subject><issn>0168-9452</issn><issn>1873-2259</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqFkU2LFDEQhhtRcFz9CUIuigu25qs73SdZhtEVBgS_rqE6qexGepIxyQy7V3-56Z1lPXpJUpWn3oL3bZqXjL5jlPXvv9VjaEfZ8TdUnFNK-7GVj5oVG5RoOe_Gx83qAXnaPMv5V4V416lV82cdQy6-HIo_IsGbfcKcfQwkOvIztZuvG3KFAYkPpCQI2cW0Q0uuEuzx6ANmYmJwmDLBcA3B1L-q4HNZ3qREgodyu6_gWwK1vKk6LsUd2SxtIDMUeN48cTBnfHF_nzU_Pm6-ry_b7ZdPn9cX29ZIykurYJgMDI7JiVsrjBCq79Q0gXXIubTjwNworJgEWgDby8FMSlLb9aAU50qcNecn3WuY9T75HaRbHcHry4utXnqU92qUcjyyyr4-sfsUfx8wF73z2eA8Q8B4yJoNw6DosIh2J9CkmHNC96DMqF7S0Xfp6MV6TYW-S0fLOvfqfgFkA7Or3hqf_w1LxTo60sp9OHFYnTl6TDobj4vPPqEp2kb_n01_Aat1peU</recordid><startdate>20030501</startdate><enddate>20030501</enddate><creator>Legrand, Valérie</creator><creator>Dalmayrac, Sylvie</creator><creator>Latché, Alain</creator><creator>Pech, Jean-Claude</creator><creator>Bouzayen, Mondher</creator><creator>Fallot, Jean</creator><creator>Torregrosa, Laurent</creator><creator>Bouquet, Alain</creator><creator>Roustan, Jean-Paul</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-1622-5610</orcidid></search><sort><creationdate>20030501</creationdate><title>Constitutive expression of Vr-ERE gene in transformed grapevines confers enhanced resistance to eutypine, a toxin from Eutypa lata</title><author>Legrand, Valérie ; Dalmayrac, Sylvie ; Latché, Alain ; Pech, Jean-Claude ; Bouzayen, Mondher ; Fallot, Jean ; Torregrosa, Laurent ; Bouquet, Alain ; Roustan, Jean-Paul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-7a8bca8f14b2dd3c337657bbadfe224d981f93d3b3edaad648cb740d56a772273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Biological and medical sciences</topic><topic>Detoxification</topic><topic>Eutypa dieback</topic><topic>Eutypa lata</topic><topic>Eutypine</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic transformation</topic><topic>Genetics</topic><topic>Genetics and breeding of economic plants</topic><topic>Grapevine</topic><topic>Life Sciences</topic><topic>Pest resistance</topic><topic>Plant pathogens</topic><topic>Plants genetics</topic><topic>Reductase</topic><topic>Toxin</topic><topic>Varietal selection. Specialized plant breeding, plant breeding aims</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Legrand, Valérie</creatorcontrib><creatorcontrib>Dalmayrac, Sylvie</creatorcontrib><creatorcontrib>Latché, Alain</creatorcontrib><creatorcontrib>Pech, Jean-Claude</creatorcontrib><creatorcontrib>Bouzayen, Mondher</creatorcontrib><creatorcontrib>Fallot, Jean</creatorcontrib><creatorcontrib>Torregrosa, Laurent</creatorcontrib><creatorcontrib>Bouquet, Alain</creatorcontrib><creatorcontrib>Roustan, Jean-Paul</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Plant science (Limerick)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Legrand, Valérie</au><au>Dalmayrac, Sylvie</au><au>Latché, Alain</au><au>Pech, Jean-Claude</au><au>Bouzayen, Mondher</au><au>Fallot, Jean</au><au>Torregrosa, Laurent</au><au>Bouquet, Alain</au><au>Roustan, Jean-Paul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Constitutive expression of Vr-ERE gene in transformed grapevines confers enhanced resistance to eutypine, a toxin from Eutypa lata</atitle><jtitle>Plant science (Limerick)</jtitle><date>2003-05-01</date><risdate>2003</risdate><volume>164</volume><issue>5</issue><spage>809</spage><epage>814</epage><pages>809-814</pages><issn>0168-9452</issn><eissn>1873-2259</eissn><coden>PLSCE4</coden><abstract>Eutypine (4-hydroxy-3-[3-methyl-3-butene-1-ynyl] benzaldehyde) is a toxin produced by
Eutypa lata, the causal agent of eutypa dieback of grapevines. Recently, a eutypine detoxifying gene (
Vr-ERE) encoding an NADPH-dependent aldehyde reductase which converts eutypine into the corresponding alcohol, eutypinol, a non-toxic form of the toxin, has been cloned. We report here the obtention of transgenic plants from grapevine rootstock 110 Richter
Vitis berlandieri×
V. rupestris that express the
Vr-ERE gene. The over-expression of
Vr-ERE gene in grapevine tissues increases their detoxification capacity. The growth and development of the transgenic plants cultured in vitro exhibiting a high Vr-ERE activity were not affected by the presence of 300 μM of toxin whereas the growth of untransformed control plants was highly inhibited. These results suggest that the
Vr-ERE gene is an efficient candidate to confer resistance to the toxin and opens new opportunities to study the role of the toxin in the development of the disease.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><doi>10.1016/S0168-9452(03)00069-4</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0003-1622-5610</orcidid></addata></record> |
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| ispartof | Plant science (Limerick), 2003-05, Vol.164 (5), p.809-814 |
| issn | 0168-9452 1873-2259 |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Agronomy. Soil science and plant productions Biological and medical sciences Detoxification Eutypa dieback Eutypa lata Eutypine Fundamental and applied biological sciences. Psychology Genetic transformation Genetics Genetics and breeding of economic plants Grapevine Life Sciences Pest resistance Plant pathogens Plants genetics Reductase Toxin Varietal selection. Specialized plant breeding, plant breeding aims |
| title | Constitutive expression of Vr-ERE gene in transformed grapevines confers enhanced resistance to eutypine, a toxin from Eutypa lata |
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