Plant growth regulator levels during maturation of larch somatic embryos
The profile of various hormones was assessed during maturation of somatic embryos of hybrid larch ( Larix kaempferi (Lamb.) Carriere × L. decidua Miller). Auxins (2,4-dichlorophenoxyacetic acid, 2,4-D; indole-3-acetic acid, IAA; indole-3-butyric acid, IBA; indole-3-acetic-aspartate, IAasp), cytokin...
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| description | The profile of various hormones was assessed during maturation of somatic embryos of hybrid larch (
Larix kaempferi (Lamb.) Carriere ×
L. decidua Miller). Auxins (2,4-dichlorophenoxyacetic acid, 2,4-D; indole-3-acetic acid, IAA; indole-3-butyric acid, IBA; indole-3-acetic-aspartate, IAasp), cytokinins (benzyl aminopurine, BAP; isopentyladenine, iP; isopentyladenosine, iPA; zeatin, Z; zeatin riboside, ZR) and abscisic acid (ABA; abscisic acid glucosyl ester, ABA-GE) were measured in somatic embryos maturing on a medium supplemented with 60 μM ABA and 1 μM IBA. These were compared to embryos and megagametophytes dissected from mature hybrid larch seed. Plant growth regulators were extracted in methanol, purified, fractionated by high pressure liquid chromatography (HPLC) and quantified by enzyme-linked immunosorbent assay (ELISA). In somatic embryos, ABA and ABA-GE rose steadily to 225 and 26 pmol·embryo
–1, respectively. In zygotic embryos and megagametophytes, abscisic acid levels were orders of magnitude lower. IBA concentration in the first week of somatic embryo maturation was 816 fmol·embryo
–1, but dropped to below detection levels within a week. IAA and IAasp concentrations rose over the course of development. Final concentration of IAA in somatic embryos (731 fmol·embryo
–1) was comparable to that found in zygotic embryos (877 fmol·embryo
–1). Cytokinins such as Z and ZR fluctuated at low concentrations (0.1–5.2 fmol·embryo
–1 range). With the exception of week 5, when levels of iP and iPA reached 20–30 fmol·embryo
–1, levels of these substances were generally within the 0.1–6.5 fmol·embryo
–1 range. Cytokinin concentrations were similar to those of mature zygotic embryos. |
| doi_str_mv | 10.1016/S0981-9428(01)01271-2 |
| format | Article |
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Larix kaempferi (Lamb.) Carriere ×
L. decidua Miller). Auxins (2,4-dichlorophenoxyacetic acid, 2,4-D; indole-3-acetic acid, IAA; indole-3-butyric acid, IBA; indole-3-acetic-aspartate, IAasp), cytokinins (benzyl aminopurine, BAP; isopentyladenine, iP; isopentyladenosine, iPA; zeatin, Z; zeatin riboside, ZR) and abscisic acid (ABA; abscisic acid glucosyl ester, ABA-GE) were measured in somatic embryos maturing on a medium supplemented with 60 μM ABA and 1 μM IBA. These were compared to embryos and megagametophytes dissected from mature hybrid larch seed. Plant growth regulators were extracted in methanol, purified, fractionated by high pressure liquid chromatography (HPLC) and quantified by enzyme-linked immunosorbent assay (ELISA). In somatic embryos, ABA and ABA-GE rose steadily to 225 and 26 pmol·embryo
–1, respectively. In zygotic embryos and megagametophytes, abscisic acid levels were orders of magnitude lower. IBA concentration in the first week of somatic embryo maturation was 816 fmol·embryo
–1, but dropped to below detection levels within a week. IAA and IAasp concentrations rose over the course of development. Final concentration of IAA in somatic embryos (731 fmol·embryo
–1) was comparable to that found in zygotic embryos (877 fmol·embryo
–1). Cytokinins such as Z and ZR fluctuated at low concentrations (0.1–5.2 fmol·embryo
–1 range). With the exception of week 5, when levels of iP and iPA reached 20–30 fmol·embryo
–1, levels of these substances were generally within the 0.1–6.5 fmol·embryo
–1 range. Cytokinin concentrations were similar to those of mature zygotic embryos.</description><identifier>ISSN: 0981-9428</identifier><identifier>EISSN: 1873-2690</identifier><identifier>DOI: 10.1016/S0981-9428(01)01271-2</identifier><identifier>CODEN: PPBIEX</identifier><language>eng</language><publisher>Paris: Elsevier Masson SAS</publisher><subject>Agronomy. Soil science and plant productions ; ARBOLES FORESTALES ; ARBRE FORESTIER ; Biochemistry, Molecular Biology ; Biological and medical sciences ; Development Biology ; Economic plant physiology ; ELISA ; EMBRION SOMATICO ; EMBRYON SOMATIQUE ; FOREST TREES ; Fructification and ripening ; Fructification, ripening. Postharvest physiology ; Fundamental and applied biological sciences. Psychology ; Growth and development ; growth regulators ; larch ; LARIX ; Life Sciences ; MADURACION ; MATURATION ; PLANT GROWTH SUBSTANCES ; Plant physiology and development ; SOMATIC EMBRYOS ; SUBSTANCE DE CROISSANCE VEGETALE ; SUSTANCIAS DE CRECIMIENTO VEGETAL ; Vegetal Biology ; Vegetative and sexual reproduction, floral biology, fructification ; zygotic embryos</subject><ispartof>Plant physiology and biochemistry, 2001-06, Vol.39 (6), p.495-502</ispartof><rights>2001 Éditions scientifiques et médicales Elsevier SAS</rights><rights>2001 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c510t-dceb491d5438871d7033df1c5835db8a7318d7ab468e3e5b255a168c7059a0493</citedby><cites>FETCH-LOGICAL-c510t-dceb491d5438871d7033df1c5835db8a7318d7ab468e3e5b255a168c7059a0493</cites><orcidid>0000-0001-7852-5868</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0981-9428(01)01271-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,778,782,883,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1037241$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02678588$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>von Aderkas, Patrick</creatorcontrib><creatorcontrib>Lelu, Marie-Anne</creatorcontrib><creatorcontrib>Label, Philippe</creatorcontrib><title>Plant growth regulator levels during maturation of larch somatic embryos</title><title>Plant physiology and biochemistry</title><description>The profile of various hormones was assessed during maturation of somatic embryos of hybrid larch (
Larix kaempferi (Lamb.) Carriere ×
L. decidua Miller). Auxins (2,4-dichlorophenoxyacetic acid, 2,4-D; indole-3-acetic acid, IAA; indole-3-butyric acid, IBA; indole-3-acetic-aspartate, IAasp), cytokinins (benzyl aminopurine, BAP; isopentyladenine, iP; isopentyladenosine, iPA; zeatin, Z; zeatin riboside, ZR) and abscisic acid (ABA; abscisic acid glucosyl ester, ABA-GE) were measured in somatic embryos maturing on a medium supplemented with 60 μM ABA and 1 μM IBA. These were compared to embryos and megagametophytes dissected from mature hybrid larch seed. Plant growth regulators were extracted in methanol, purified, fractionated by high pressure liquid chromatography (HPLC) and quantified by enzyme-linked immunosorbent assay (ELISA). In somatic embryos, ABA and ABA-GE rose steadily to 225 and 26 pmol·embryo
–1, respectively. In zygotic embryos and megagametophytes, abscisic acid levels were orders of magnitude lower. IBA concentration in the first week of somatic embryo maturation was 816 fmol·embryo
–1, but dropped to below detection levels within a week. IAA and IAasp concentrations rose over the course of development. Final concentration of IAA in somatic embryos (731 fmol·embryo
–1) was comparable to that found in zygotic embryos (877 fmol·embryo
–1). Cytokinins such as Z and ZR fluctuated at low concentrations (0.1–5.2 fmol·embryo
–1 range). With the exception of week 5, when levels of iP and iPA reached 20–30 fmol·embryo
–1, levels of these substances were generally within the 0.1–6.5 fmol·embryo
–1 range. Cytokinin concentrations were similar to those of mature zygotic embryos.</description><subject>Agronomy. Soil science and plant productions</subject><subject>ARBOLES FORESTALES</subject><subject>ARBRE FORESTIER</subject><subject>Biochemistry, Molecular Biology</subject><subject>Biological and medical sciences</subject><subject>Development Biology</subject><subject>Economic plant physiology</subject><subject>ELISA</subject><subject>EMBRION SOMATICO</subject><subject>EMBRYON SOMATIQUE</subject><subject>FOREST TREES</subject><subject>Fructification and ripening</subject><subject>Fructification, ripening. Postharvest physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Growth and development</subject><subject>growth regulators</subject><subject>larch</subject><subject>LARIX</subject><subject>Life Sciences</subject><subject>MADURACION</subject><subject>MATURATION</subject><subject>PLANT GROWTH SUBSTANCES</subject><subject>Plant physiology and development</subject><subject>SOMATIC EMBRYOS</subject><subject>SUBSTANCE DE CROISSANCE VEGETALE</subject><subject>SUSTANCIAS DE CRECIMIENTO VEGETAL</subject><subject>Vegetal Biology</subject><subject>Vegetative and sexual reproduction, floral biology, fructification</subject><subject>zygotic embryos</subject><issn>0981-9428</issn><issn>1873-2690</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNqFkE1r3DAQhkVpodu0PyGgQw_Nwe2MZFnyqYSQdAsLLf04i7Ek76p4rSB5t-Tf145L6K2ngZnnfRkexi4R3iNg8-E7tAarthbmHeAVoNBYiWdsg0bLSjQtPGebJ-Qle1XKLwAQtZYbtv060DjxfU6_pwPPYX8aaEqZD-EchsL9Kcdxz480nTJNMY089Xyg7A68pHkbHQ_HLj-k8pq96Gko4c3fecF-3t3-uNlWuy-fPt9c7yqnEKbKu9DVLXpVS2M0eg1S-h6dMlL5zpCWaLymrm5MkEF1QinCxjgNqiWoW3nBrtbeAw32Pscj5QebKNrt9c4uOxCNNsqYM86sWlmXUyk59E8BBLuos4_q7OLFAtpHdVbMubdr7p6Ko6HPNLpY_glLLeql_nLFekqW9nlG7r6J2ewiF5aaj-t9NhnOMWRbXAyjCz7m4CbrU_zPI38ADg6KVA</recordid><startdate>20010601</startdate><enddate>20010601</enddate><creator>von Aderkas, Patrick</creator><creator>Lelu, Marie-Anne</creator><creator>Label, Philippe</creator><general>Elsevier Masson SAS</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-7852-5868</orcidid></search><sort><creationdate>20010601</creationdate><title>Plant growth regulator levels during maturation of larch somatic embryos</title><author>von Aderkas, Patrick ; Lelu, Marie-Anne ; Label, Philippe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c510t-dceb491d5438871d7033df1c5835db8a7318d7ab468e3e5b255a168c7059a0493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>ARBOLES FORESTALES</topic><topic>ARBRE FORESTIER</topic><topic>Biochemistry, Molecular Biology</topic><topic>Biological and medical sciences</topic><topic>Development Biology</topic><topic>Economic plant physiology</topic><topic>ELISA</topic><topic>EMBRION SOMATICO</topic><topic>EMBRYON SOMATIQUE</topic><topic>FOREST TREES</topic><topic>Fructification and ripening</topic><topic>Fructification, ripening. Postharvest physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Growth and development</topic><topic>growth regulators</topic><topic>larch</topic><topic>LARIX</topic><topic>Life Sciences</topic><topic>MADURACION</topic><topic>MATURATION</topic><topic>PLANT GROWTH SUBSTANCES</topic><topic>Plant physiology and development</topic><topic>SOMATIC EMBRYOS</topic><topic>SUBSTANCE DE CROISSANCE VEGETALE</topic><topic>SUSTANCIAS DE CRECIMIENTO VEGETAL</topic><topic>Vegetal Biology</topic><topic>Vegetative and sexual reproduction, floral biology, fructification</topic><topic>zygotic embryos</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>von Aderkas, Patrick</creatorcontrib><creatorcontrib>Lelu, Marie-Anne</creatorcontrib><creatorcontrib>Label, Philippe</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Plant physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>von Aderkas, Patrick</au><au>Lelu, Marie-Anne</au><au>Label, Philippe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Plant growth regulator levels during maturation of larch somatic embryos</atitle><jtitle>Plant physiology and biochemistry</jtitle><date>2001-06-01</date><risdate>2001</risdate><volume>39</volume><issue>6</issue><spage>495</spage><epage>502</epage><pages>495-502</pages><issn>0981-9428</issn><eissn>1873-2690</eissn><coden>PPBIEX</coden><abstract>The profile of various hormones was assessed during maturation of somatic embryos of hybrid larch (
Larix kaempferi (Lamb.) Carriere ×
L. decidua Miller). Auxins (2,4-dichlorophenoxyacetic acid, 2,4-D; indole-3-acetic acid, IAA; indole-3-butyric acid, IBA; indole-3-acetic-aspartate, IAasp), cytokinins (benzyl aminopurine, BAP; isopentyladenine, iP; isopentyladenosine, iPA; zeatin, Z; zeatin riboside, ZR) and abscisic acid (ABA; abscisic acid glucosyl ester, ABA-GE) were measured in somatic embryos maturing on a medium supplemented with 60 μM ABA and 1 μM IBA. These were compared to embryos and megagametophytes dissected from mature hybrid larch seed. Plant growth regulators were extracted in methanol, purified, fractionated by high pressure liquid chromatography (HPLC) and quantified by enzyme-linked immunosorbent assay (ELISA). In somatic embryos, ABA and ABA-GE rose steadily to 225 and 26 pmol·embryo
–1, respectively. In zygotic embryos and megagametophytes, abscisic acid levels were orders of magnitude lower. IBA concentration in the first week of somatic embryo maturation was 816 fmol·embryo
–1, but dropped to below detection levels within a week. IAA and IAasp concentrations rose over the course of development. Final concentration of IAA in somatic embryos (731 fmol·embryo
–1) was comparable to that found in zygotic embryos (877 fmol·embryo
–1). Cytokinins such as Z and ZR fluctuated at low concentrations (0.1–5.2 fmol·embryo
–1 range). With the exception of week 5, when levels of iP and iPA reached 20–30 fmol·embryo
–1, levels of these substances were generally within the 0.1–6.5 fmol·embryo
–1 range. Cytokinin concentrations were similar to those of mature zygotic embryos.</abstract><cop>Paris</cop><pub>Elsevier Masson SAS</pub><doi>10.1016/S0981-9428(01)01271-2</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-7852-5868</orcidid></addata></record> |
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| ispartof | Plant physiology and biochemistry, 2001-06, Vol.39 (6), p.495-502 |
| issn | 0981-9428 1873-2690 |
| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | Agronomy. Soil science and plant productions ARBOLES FORESTALES ARBRE FORESTIER Biochemistry, Molecular Biology Biological and medical sciences Development Biology Economic plant physiology ELISA EMBRION SOMATICO EMBRYON SOMATIQUE FOREST TREES Fructification and ripening Fructification, ripening. Postharvest physiology Fundamental and applied biological sciences. Psychology Growth and development growth regulators larch LARIX Life Sciences MADURACION MATURATION PLANT GROWTH SUBSTANCES Plant physiology and development SOMATIC EMBRYOS SUBSTANCE DE CROISSANCE VEGETALE SUSTANCIAS DE CRECIMIENTO VEGETAL Vegetal Biology Vegetative and sexual reproduction, floral biology, fructification zygotic embryos |
| title | Plant growth regulator levels during maturation of larch somatic embryos |
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