Post-transcriptional gene silencing in plum pox virus resistant transgenic european plum containing the plum pox potyvirus coat protein gene
Transgenic plums containing the plum pox potyvirus coat protein (PPV-CP) gene were inoculated with PPV. Infection was monitored by evaluating symptoms, ELISA, and IC-RT-PCR. Transgenic clone C5 was highly resistant to PPV during four years of testing and displayed characteristics typical of post-tra...
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| Veröffentlicht in: | Transgenic research 2001-06, Vol.10 (3), p.201-209 |
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| creator | SCORZA, Ralph CALLAHAN, Ann LEVY, Laurene DAMSTEEGT, Vern WEBB, Kevin RAVELONANDRO, Michel |
| description | Transgenic plums containing the plum pox potyvirus coat protein (PPV-CP) gene were inoculated with PPV. Infection was monitored by evaluating symptoms, ELISA, and IC-RT-PCR. Transgenic clone C5 was highly resistant to PPV during four years of testing and displayed characteristics typical of post-transcriptional gene silencing (PTGS), including a high level of transgene transcription in the nucleus, low levels of transgene mRNA in the cytoplasm, a complex multicopy transgene insertion with aberrant copies, and methylation of the silenced PPV-CP transgene. The PPV-CP transgene was also methylated in seedlings of C5 and these seedlings were resistant to PPV. Our results show, for the first time, that PTGS functions as a mechanism for virus resistance in a woody perennial species. |
| doi_str_mv | 10.1023/A:1016644823203 |
| format | Article |
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Infection was monitored by evaluating symptoms, ELISA, and IC-RT-PCR. Transgenic clone C5 was highly resistant to PPV during four years of testing and displayed characteristics typical of post-transcriptional gene silencing (PTGS), including a high level of transgene transcription in the nucleus, low levels of transgene mRNA in the cytoplasm, a complex multicopy transgene insertion with aberrant copies, and methylation of the silenced PPV-CP transgene. The PPV-CP transgene was also methylated in seedlings of C5 and these seedlings were resistant to PPV. Our results show, for the first time, that PTGS functions as a mechanism for virus resistance in a woody perennial species.</description><identifier>ISSN: 0962-8819</identifier><identifier>EISSN: 1573-9368</identifier><identifier>DOI: 10.1023/A:1016644823203</identifier><identifier>PMID: 11437277</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Agronomy. Soil science and plant productions ; Biological and medical sciences ; Biotechnology ; Blotting, Southern ; Capsid - genetics ; Capsid - metabolism ; Coat protein ; Cytoplasm ; Disease resistance ; DNA Methylation ; Enzyme-Linked Immunosorbent Assay ; Fruit - genetics ; Fruit - metabolism ; Fruit - virology ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Viral ; Gene Silencing ; Genes, Viral - genetics ; Genetic engineering ; Genetic Predisposition to Disease ; Genetic technics ; Genetics and breeding of economic plants ; Insertion ; Life Sciences ; Methods. Procedures. Technologies ; Pest resistance ; Plant Diseases - genetics ; Plant Diseases - virology ; Plant pathogens ; Plant virus diseases ; Plant viruses ; Plants, Genetically Modified ; Plum pox ; plum pox potyvirus ; Plum pox virus ; Plum Pox Virus - genetics ; Plum Pox Virus - physiology ; Polymerase Chain Reaction ; Post-transcription ; Potyvirus ; Proteins ; Prunus ; Prunus domestica domestica ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Seedlings ; Transcription, Genetic - genetics ; Transgenes - genetics ; Transgenic animals and transgenic plants ; Transgenic plants ; Varietal selection. Specialized plant breeding, plant breeding aims</subject><ispartof>Transgenic research, 2001-06, Vol.10 (3), p.201-209</ispartof><rights>2001 INIST-CNRS</rights><rights>Kluwer Academic Publishers 2001.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c402t-27ed98fe7b637137524e40e519182a3252163d5ba5d9a9a4c1851b0bf578ad963</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1102723$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11437277$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02676903$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>SCORZA, Ralph</creatorcontrib><creatorcontrib>CALLAHAN, Ann</creatorcontrib><creatorcontrib>LEVY, Laurene</creatorcontrib><creatorcontrib>DAMSTEEGT, Vern</creatorcontrib><creatorcontrib>WEBB, Kevin</creatorcontrib><creatorcontrib>RAVELONANDRO, Michel</creatorcontrib><title>Post-transcriptional gene silencing in plum pox virus resistant transgenic european plum containing the plum pox potyvirus coat protein gene</title><title>Transgenic research</title><addtitle>Transgenic Res</addtitle><description>Transgenic plums containing the plum pox potyvirus coat protein (PPV-CP) gene were inoculated with PPV. Infection was monitored by evaluating symptoms, ELISA, and IC-RT-PCR. Transgenic clone C5 was highly resistant to PPV during four years of testing and displayed characteristics typical of post-transcriptional gene silencing (PTGS), including a high level of transgene transcription in the nucleus, low levels of transgene mRNA in the cytoplasm, a complex multicopy transgene insertion with aberrant copies, and methylation of the silenced PPV-CP transgene. The PPV-CP transgene was also methylated in seedlings of C5 and these seedlings were resistant to PPV. Our results show, for the first time, that PTGS functions as a mechanism for virus resistance in a woody perennial species.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Blotting, Southern</subject><subject>Capsid - genetics</subject><subject>Capsid - metabolism</subject><subject>Coat protein</subject><subject>Cytoplasm</subject><subject>Disease resistance</subject><subject>DNA Methylation</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fruit - genetics</subject><subject>Fruit - metabolism</subject><subject>Fruit - virology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Viral</subject><subject>Gene Silencing</subject><subject>Genes, Viral - genetics</subject><subject>Genetic engineering</subject><subject>Genetic Predisposition to Disease</subject><subject>Genetic technics</subject><subject>Genetics and breeding of economic plants</subject><subject>Insertion</subject><subject>Life Sciences</subject><subject>Methods. Procedures. Technologies</subject><subject>Pest resistance</subject><subject>Plant Diseases - genetics</subject><subject>Plant Diseases - virology</subject><subject>Plant pathogens</subject><subject>Plant virus diseases</subject><subject>Plant viruses</subject><subject>Plants, Genetically Modified</subject><subject>Plum pox</subject><subject>plum pox potyvirus</subject><subject>Plum pox virus</subject><subject>Plum Pox Virus - genetics</subject><subject>Plum Pox Virus - physiology</subject><subject>Polymerase Chain Reaction</subject><subject>Post-transcription</subject><subject>Potyvirus</subject><subject>Proteins</subject><subject>Prunus</subject><subject>Prunus domestica domestica</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Seedlings</subject><subject>Transcription, Genetic - genetics</subject><subject>Transgenes - genetics</subject><subject>Transgenic animals and transgenic plants</subject><subject>Transgenic plants</subject><subject>Varietal selection. 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Soil science and plant productions</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Blotting, Southern</topic><topic>Capsid - genetics</topic><topic>Capsid - metabolism</topic><topic>Coat protein</topic><topic>Cytoplasm</topic><topic>Disease resistance</topic><topic>DNA Methylation</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fruit - genetics</topic><topic>Fruit - metabolism</topic><topic>Fruit - virology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Viral</topic><topic>Gene Silencing</topic><topic>Genes, Viral - genetics</topic><topic>Genetic engineering</topic><topic>Genetic Predisposition to Disease</topic><topic>Genetic technics</topic><topic>Genetics and breeding of economic plants</topic><topic>Insertion</topic><topic>Life Sciences</topic><topic>Methods. Procedures. Technologies</topic><topic>Pest resistance</topic><topic>Plant Diseases - genetics</topic><topic>Plant Diseases - virology</topic><topic>Plant pathogens</topic><topic>Plant virus diseases</topic><topic>Plant viruses</topic><topic>Plants, Genetically Modified</topic><topic>Plum pox</topic><topic>plum pox potyvirus</topic><topic>Plum pox virus</topic><topic>Plum Pox Virus - genetics</topic><topic>Plum Pox Virus - physiology</topic><topic>Polymerase Chain Reaction</topic><topic>Post-transcription</topic><topic>Potyvirus</topic><topic>Proteins</topic><topic>Prunus</topic><topic>Prunus domestica domestica</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Seedlings</topic><topic>Transcription, Genetic - genetics</topic><topic>Transgenes - genetics</topic><topic>Transgenic animals and transgenic plants</topic><topic>Transgenic plants</topic><topic>Varietal selection. 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Infection was monitored by evaluating symptoms, ELISA, and IC-RT-PCR. Transgenic clone C5 was highly resistant to PPV during four years of testing and displayed characteristics typical of post-transcriptional gene silencing (PTGS), including a high level of transgene transcription in the nucleus, low levels of transgene mRNA in the cytoplasm, a complex multicopy transgene insertion with aberrant copies, and methylation of the silenced PPV-CP transgene. The PPV-CP transgene was also methylated in seedlings of C5 and these seedlings were resistant to PPV. Our results show, for the first time, that PTGS functions as a mechanism for virus resistance in a woody perennial species.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>11437277</pmid><doi>10.1023/A:1016644823203</doi><tpages>9</tpages></addata></record> |
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| ispartof | Transgenic research, 2001-06, Vol.10 (3), p.201-209 |
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| subjects | Agronomy. Soil science and plant productions Biological and medical sciences Biotechnology Blotting, Southern Capsid - genetics Capsid - metabolism Coat protein Cytoplasm Disease resistance DNA Methylation Enzyme-Linked Immunosorbent Assay Fruit - genetics Fruit - metabolism Fruit - virology Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Viral Gene Silencing Genes, Viral - genetics Genetic engineering Genetic Predisposition to Disease Genetic technics Genetics and breeding of economic plants Insertion Life Sciences Methods. Procedures. Technologies Pest resistance Plant Diseases - genetics Plant Diseases - virology Plant pathogens Plant virus diseases Plant viruses Plants, Genetically Modified Plum pox plum pox potyvirus Plum pox virus Plum Pox Virus - genetics Plum Pox Virus - physiology Polymerase Chain Reaction Post-transcription Potyvirus Proteins Prunus Prunus domestica domestica Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics RNA, Messenger - metabolism Seedlings Transcription, Genetic - genetics Transgenes - genetics Transgenic animals and transgenic plants Transgenic plants Varietal selection. Specialized plant breeding, plant breeding aims |
| title | Post-transcriptional gene silencing in plum pox virus resistant transgenic european plum containing the plum pox potyvirus coat protein gene |
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