Functional analysis of the ALD gene family of Saccharomyces cerevisiae during anaerobic growth on glucose: the NADP+-dependent Ald6p and Ald5p isoforms play a major role in acetate formation
UMR Sciences pour l' nologie, Microbiologie et Technologie des Fermentations, INRA, 2 Place Viala, F-34060 Montpellier Cedex 1, France Correspondence Sylvie Dequin dequin{at}ensam.inra.fr In Saccharomyces cerevisiae , acetate is formed by acetaldehyde dehydrogenase (ACDH), a key enzyme of the p...
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| Veröffentlicht in: | Microbiology (Society for General Microbiology) 2004-07, Vol.150 (7), p.2209-2220 |
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| creator | Saint-Prix, Florence Bonquist, Linda Dequin, Sylvie |
| description | UMR Sciences pour l' nologie, Microbiologie et Technologie des Fermentations, INRA, 2 Place Viala, F-34060 Montpellier Cedex 1, France
Correspondence Sylvie Dequin dequin{at}ensam.inra.fr
In Saccharomyces cerevisiae , acetate is formed by acetaldehyde dehydrogenase (ACDH), a key enzyme of the pyruvate dehydrogenase (PDH) bypass, which fulfils the essential task of generating acetyl-CoA in the cytosol. The role of the five members of the ACDH family ( ALD genes) was investigated during anaerobic growth on glucose. Single and multiple ald mutants were generated in the wine-yeast-derived V5 and laboratory CEN.PK strains and analysed under standard (YPD 5 % glucose) and wine (MS 20 % glucose) fermentation conditions. The deletion of ALD6 and ALD5 decreased acetate formation in both strains, demonstrating for the first time that the mitochondrial Ald5p isoform is involved in the biosynthesis of acetate during anaerobic growth on glucose. Acetate production of the ald4 mutant was slightly decreased in the CEN.PK strain during growth on YPD only. In contrast, the deletion of ALD2 or ALD3 had no effect on acetate production. The absence of Ald6p was compensated by the mitochondrial isoforms and this involves the transcriptional activation of ALD4 . Consistent with this, growth retardation was observed in ald6 ald4 , and this effect was amplified by the additional deletion of ALD5 . A ald null mutant, devoid of ACDH activity, was viable and produced similar levels of acetate to the ald6 ald4 ald5 strain, excluding a role of Ald2p and Ald3p. Thus, acetate is mainly produced by the cytosolic PDH bypass via Ald6p and by a mitochondrial route involving Ald5p. An unknown alternative pathway can compensate for the loss of Ald6p, Ald4p and Ald5p.
Abbreviations: ACDH, acetaldehyde dehydrogenase; HA, haemagglutinin; PDC, pyruvate decarboxylase; PDH, pyruvate dehydrogenase |
| doi_str_mv | 10.1099/mic.0.26999-0 |
| format | Article |
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Correspondence Sylvie Dequin dequin{at}ensam.inra.fr
In Saccharomyces cerevisiae , acetate is formed by acetaldehyde dehydrogenase (ACDH), a key enzyme of the pyruvate dehydrogenase (PDH) bypass, which fulfils the essential task of generating acetyl-CoA in the cytosol. The role of the five members of the ACDH family ( ALD genes) was investigated during anaerobic growth on glucose. Single and multiple ald mutants were generated in the wine-yeast-derived V5 and laboratory CEN.PK strains and analysed under standard (YPD 5 % glucose) and wine (MS 20 % glucose) fermentation conditions. The deletion of ALD6 and ALD5 decreased acetate formation in both strains, demonstrating for the first time that the mitochondrial Ald5p isoform is involved in the biosynthesis of acetate during anaerobic growth on glucose. Acetate production of the ald4 mutant was slightly decreased in the CEN.PK strain during growth on YPD only. In contrast, the deletion of ALD2 or ALD3 had no effect on acetate production. The absence of Ald6p was compensated by the mitochondrial isoforms and this involves the transcriptional activation of ALD4 . Consistent with this, growth retardation was observed in ald6 ald4 , and this effect was amplified by the additional deletion of ALD5 . A ald null mutant, devoid of ACDH activity, was viable and produced similar levels of acetate to the ald6 ald4 ald5 strain, excluding a role of Ald2p and Ald3p. Thus, acetate is mainly produced by the cytosolic PDH bypass via Ald6p and by a mitochondrial route involving Ald5p. An unknown alternative pathway can compensate for the loss of Ald6p, Ald4p and Ald5p.
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Correspondence Sylvie Dequin dequin{at}ensam.inra.fr
In Saccharomyces cerevisiae , acetate is formed by acetaldehyde dehydrogenase (ACDH), a key enzyme of the pyruvate dehydrogenase (PDH) bypass, which fulfils the essential task of generating acetyl-CoA in the cytosol. The role of the five members of the ACDH family ( ALD genes) was investigated during anaerobic growth on glucose. Single and multiple ald mutants were generated in the wine-yeast-derived V5 and laboratory CEN.PK strains and analysed under standard (YPD 5 % glucose) and wine (MS 20 % glucose) fermentation conditions. The deletion of ALD6 and ALD5 decreased acetate formation in both strains, demonstrating for the first time that the mitochondrial Ald5p isoform is involved in the biosynthesis of acetate during anaerobic growth on glucose. Acetate production of the ald4 mutant was slightly decreased in the CEN.PK strain during growth on YPD only. In contrast, the deletion of ALD2 or ALD3 had no effect on acetate production. The absence of Ald6p was compensated by the mitochondrial isoforms and this involves the transcriptional activation of ALD4 . Consistent with this, growth retardation was observed in ald6 ald4 , and this effect was amplified by the additional deletion of ALD5 . A ald null mutant, devoid of ACDH activity, was viable and produced similar levels of acetate to the ald6 ald4 ald5 strain, excluding a role of Ald2p and Ald3p. Thus, acetate is mainly produced by the cytosolic PDH bypass via Ald6p and by a mitochondrial route involving Ald5p. An unknown alternative pathway can compensate for the loss of Ald6p, Ald4p and Ald5p.
Abbreviations: ACDH, acetaldehyde dehydrogenase; HA, haemagglutinin; PDC, pyruvate decarboxylase; PDH, pyruvate dehydrogenase</description><subject>Acetates - metabolism</subject><subject>Aldehyde Oxidoreductases - genetics</subject><subject>Aldehyde Oxidoreductases - metabolism</subject><subject>Anaerobiosis</subject><subject>Biological and medical sciences</subject><subject>Culture Media</subject><subject>Fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal plant pathogens</subject><subject>Gene Deletion</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Genes, Fungal</subject><subject>Glucose - metabolism</subject><subject>Isoenzymes - metabolism</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Microbiology and Parasitology</subject><subject>Miscellaneous</subject><subject>Multigene Family</subject><subject>Mycological methods and techniques used in mycology</subject><subject>Mycology</subject><subject>NADP - metabolism</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - growth & development</subject><issn>1350-0872</issn><issn>1465-2080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkcFu1DAQhiMEoqVw5Ip8AQmhLHYce2tuq5ZSpBUgAWdr1h7vunLi1E5a7cvxbDjdFfRij8bf_DPjv6peM7pgVKmPnTcLumikUqqmT6pT1kpRN_ScPi0xF7Sm58vmpHqR8w2l5ZGy59UJE42QQvLT6s_V1JvRxx4CgXLss88kOjLukKzWl2SLPRIHnQ_7Of0TjNlBit3eYCYGE9757AGJnZLvt7MEprjxhmxTvB93JPZkGyYTM3560Py2uvzxobY4YG-xH8kqWDmUMjtHYiA-RxdTl8kQYE-AdHATE0kxIPE9AYMjjGWggsA89cvqmYOQ8dXxPqt-X33-dXFdr79_-XqxWtemVc1YcxCOmobzFjfGCWedFE45a5lrRPmL842UyqLDVjpDN0q1rXIUKDRcWmo5P6veH3R3EPSQfAdpryN4fb1a6zlHG7kUjMs7Vth3B3ZI8XbCPOrOZ4MhQI9xylrKJROSzaL1ATQp5pzQ_VNmVM_mlkKjqX4wV9PCvzkKT5sO7X_66GYB3h4ByAaCS9Abnx9xSsiWq0fb-O3u3ifUxebSa3Yuzk2ZoHqpm4Yq_hf-wL2Z</recordid><startdate>20040701</startdate><enddate>20040701</enddate><creator>Saint-Prix, Florence</creator><creator>Bonquist, Linda</creator><creator>Dequin, Sylvie</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><general>Microbiology Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-9114-2324</orcidid></search><sort><creationdate>20040701</creationdate><title>Functional analysis of the ALD gene family of Saccharomyces cerevisiae during anaerobic growth on glucose: the NADP+-dependent Ald6p and Ald5p isoforms play a major role in acetate formation</title><author>Saint-Prix, Florence ; Bonquist, Linda ; Dequin, Sylvie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c492t-3a5f0c2334ebcf5fdf65f9fdd1f255258b669defe46fc0b99449f0a0a236d0d33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Acetates - metabolism</topic><topic>Aldehyde Oxidoreductases - genetics</topic><topic>Aldehyde Oxidoreductases - metabolism</topic><topic>Anaerobiosis</topic><topic>Biological and medical sciences</topic><topic>Culture Media</topic><topic>Fermentation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal plant pathogens</topic><topic>Gene Deletion</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Genes, Fungal</topic><topic>Glucose - metabolism</topic><topic>Isoenzymes - metabolism</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Microbiology and Parasitology</topic><topic>Miscellaneous</topic><topic>Multigene Family</topic><topic>Mycological methods and techniques used in mycology</topic><topic>Mycology</topic><topic>NADP - metabolism</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - growth & development</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saint-Prix, Florence</creatorcontrib><creatorcontrib>Bonquist, Linda</creatorcontrib><creatorcontrib>Dequin, Sylvie</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Microbiology (Society for General Microbiology)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saint-Prix, Florence</au><au>Bonquist, Linda</au><au>Dequin, Sylvie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Functional analysis of the ALD gene family of Saccharomyces cerevisiae during anaerobic growth on glucose: the NADP+-dependent Ald6p and Ald5p isoforms play a major role in acetate formation</atitle><jtitle>Microbiology (Society for General Microbiology)</jtitle><addtitle>Microbiology</addtitle><date>2004-07-01</date><risdate>2004</risdate><volume>150</volume><issue>7</issue><spage>2209</spage><epage>2220</epage><pages>2209-2220</pages><issn>1350-0872</issn><eissn>1465-2080</eissn><abstract>UMR Sciences pour l' nologie, Microbiologie et Technologie des Fermentations, INRA, 2 Place Viala, F-34060 Montpellier Cedex 1, France
Correspondence Sylvie Dequin dequin{at}ensam.inra.fr
In Saccharomyces cerevisiae , acetate is formed by acetaldehyde dehydrogenase (ACDH), a key enzyme of the pyruvate dehydrogenase (PDH) bypass, which fulfils the essential task of generating acetyl-CoA in the cytosol. The role of the five members of the ACDH family ( ALD genes) was investigated during anaerobic growth on glucose. Single and multiple ald mutants were generated in the wine-yeast-derived V5 and laboratory CEN.PK strains and analysed under standard (YPD 5 % glucose) and wine (MS 20 % glucose) fermentation conditions. The deletion of ALD6 and ALD5 decreased acetate formation in both strains, demonstrating for the first time that the mitochondrial Ald5p isoform is involved in the biosynthesis of acetate during anaerobic growth on glucose. Acetate production of the ald4 mutant was slightly decreased in the CEN.PK strain during growth on YPD only. In contrast, the deletion of ALD2 or ALD3 had no effect on acetate production. The absence of Ald6p was compensated by the mitochondrial isoforms and this involves the transcriptional activation of ALD4 . Consistent with this, growth retardation was observed in ald6 ald4 , and this effect was amplified by the additional deletion of ALD5 . A ald null mutant, devoid of ACDH activity, was viable and produced similar levels of acetate to the ald6 ald4 ald5 strain, excluding a role of Ald2p and Ald3p. Thus, acetate is mainly produced by the cytosolic PDH bypass via Ald6p and by a mitochondrial route involving Ald5p. An unknown alternative pathway can compensate for the loss of Ald6p, Ald4p and Ald5p.
Abbreviations: ACDH, acetaldehyde dehydrogenase; HA, haemagglutinin; PDC, pyruvate decarboxylase; PDH, pyruvate dehydrogenase</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>15256563</pmid><doi>10.1099/mic.0.26999-0</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-9114-2324</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acetates - metabolism Aldehyde Oxidoreductases - genetics Aldehyde Oxidoreductases - metabolism Anaerobiosis Biological and medical sciences Culture Media Fermentation Fundamental and applied biological sciences. Psychology Fungal plant pathogens Gene Deletion Gene Expression Regulation, Fungal Genes, Fungal Glucose - metabolism Isoenzymes - metabolism Life Sciences Microbiology Microbiology and Parasitology Miscellaneous Multigene Family Mycological methods and techniques used in mycology Mycology NADP - metabolism Phytopathology. Animal pests. Plant and forest protection Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - growth & development |
| title | Functional analysis of the ALD gene family of Saccharomyces cerevisiae during anaerobic growth on glucose: the NADP+-dependent Ald6p and Ald5p isoforms play a major role in acetate formation |
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