Characterization of AtCHX17, a member of the cation/H+ exchangers, CHX family, from Arabidopsis thaliana suggests a role in K+ homeostasis
The Arabidopsis genome contains many sequences annotated as encoding H+-coupled cotransporters. Among those are the members of the cation:proton antiporter-2 (CPA2) family (or CHX family), predicted to encode Na+,K+/H+ antiporters. AtCHX17, a member of the CPA2 family, was selected for expression st...
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| Veröffentlicht in: | The Plant journal : for cell and molecular biology 2004-09, Vol.39 (6), p.834-846 |
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| container_title | The Plant journal : for cell and molecular biology |
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| creator | Cellier, F Conejero, G Ricaud, L Luu, D.T Lepetit, M Gosti, F Casse, F |
| description | The Arabidopsis genome contains many sequences annotated as encoding H+-coupled cotransporters. Among those are the members of the cation:proton antiporter-2 (CPA2) family (or CHX family), predicted to encode Na+,K+/H+ antiporters. AtCHX17, a member of the CPA2 family, was selected for expression studies, and phenotypic analysis of knockout mutants was performed. AtCHX17 expression was only detected in roots. The gene was strongly induced by salt stress, potassium starvation, abscisic acid (ABA) and external acidic pH. Using the β-glucuronidase reporter gene strategy and in situ RT-PCR experiments, we have found that AtCHX17 was expressed preferentially in epidermal and cortical cells of the mature root zones. Knockout mutants accumulated less K+ in roots in response to salt stress and potassium starvation compared with the wild type. These data support the hypothesis that AtCHX17 is involved in K+ acquisition and homeostasis. |
| doi_str_mv | 10.1111/j.1365-313x.2004.02177.x |
| format | Article |
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Among those are the members of the cation:proton antiporter-2 (CPA2) family (or CHX family), predicted to encode Na+,K+/H+ antiporters. AtCHX17, a member of the CPA2 family, was selected for expression studies, and phenotypic analysis of knockout mutants was performed. AtCHX17 expression was only detected in roots. The gene was strongly induced by salt stress, potassium starvation, abscisic acid (ABA) and external acidic pH. Using the β-glucuronidase reporter gene strategy and in situ RT-PCR experiments, we have found that AtCHX17 was expressed preferentially in epidermal and cortical cells of the mature root zones. Knockout mutants accumulated less K+ in roots in response to salt stress and potassium starvation compared with the wild type. These data support the hypothesis that AtCHX17 is involved in K+ acquisition and homeostasis.</description><identifier>ISSN: 0960-7412</identifier><identifier>EISSN: 1365-313X</identifier><identifier>DOI: 10.1111/j.1365-313x.2004.02177.x</identifier><identifier>PMID: 15341627</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>abscisic acid ; alleles ; Amino Acid Sequence ; amino acid sequences ; antiporters ; Arabidopsis ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis Proteins - chemistry ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; AtCHX17 gene ; Base Sequence ; beta-glucuronidase ; Biological and medical sciences ; Cell physiology ; CHX transporters ; Conserved Sequence ; cortex ; DNA Primers ; epidermis (plant) ; Fundamental and applied biological sciences. Psychology ; gene expression regulation ; genetic complementation ; Genetics ; Glucuronidase - genetics ; Homeostasis ; hydroponics ; K+ transport ; K+‐starvation ; knockout mutants ; Life Sciences ; Molecular Sequence Data ; Na+,K+/H+ antiporters ; nucleotide sequences ; phenotypic variation ; plant morphology ; Plant physiology and development ; plant proteins ; Plants genetics ; Plants, Genetically Modified - metabolism ; Plasma membrane and permeation ; potassium ; Potassium - metabolism ; reporter genes ; roots ; Saccharomyces cerevisiae ; salt stress ; Sequence Alignment ; Sequence Homology, Amino Acid ; signal transduction ; sodium, potassium-hydrogen antiporters ; Sodium-Hydrogen Exchangers - chemistry ; Sodium-Hydrogen Exchangers - genetics ; Sodium-Hydrogen Exchangers - metabolism ; tissue distribution ; transgenic plants</subject><ispartof>The Plant journal : for cell and molecular biology, 2004-09, Vol.39 (6), p.834-846</ispartof><rights>2004 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5697-840162c6ccb36143f929babedd0d8c037786691a85030475b5a20f5e0c52ffca3</citedby><cites>FETCH-LOGICAL-c5697-840162c6ccb36143f929babedd0d8c037786691a85030475b5a20f5e0c52ffca3</cites><orcidid>0000-0002-0372-5985 ; 0000-0003-0559-5317</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-313X.2004.02177.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-313X.2004.02177.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,1427,27903,27904,45553,45554,46387,46811</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16100252$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15341627$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02673157$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Cellier, F</creatorcontrib><creatorcontrib>Conejero, G</creatorcontrib><creatorcontrib>Ricaud, L</creatorcontrib><creatorcontrib>Luu, D.T</creatorcontrib><creatorcontrib>Lepetit, M</creatorcontrib><creatorcontrib>Gosti, F</creatorcontrib><creatorcontrib>Casse, F</creatorcontrib><title>Characterization of AtCHX17, a member of the cation/H+ exchangers, CHX family, from Arabidopsis thaliana suggests a role in K+ homeostasis</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>The Arabidopsis genome contains many sequences annotated as encoding H+-coupled cotransporters. Among those are the members of the cation:proton antiporter-2 (CPA2) family (or CHX family), predicted to encode Na+,K+/H+ antiporters. AtCHX17, a member of the CPA2 family, was selected for expression studies, and phenotypic analysis of knockout mutants was performed. AtCHX17 expression was only detected in roots. The gene was strongly induced by salt stress, potassium starvation, abscisic acid (ABA) and external acidic pH. Using the β-glucuronidase reporter gene strategy and in situ RT-PCR experiments, we have found that AtCHX17 was expressed preferentially in epidermal and cortical cells of the mature root zones. Knockout mutants accumulated less K+ in roots in response to salt stress and potassium starvation compared with the wild type. These data support the hypothesis that AtCHX17 is involved in K+ acquisition and homeostasis.</description><subject>abscisic acid</subject><subject>alleles</subject><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>antiporters</subject><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - chemistry</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>AtCHX17 gene</subject><subject>Base Sequence</subject><subject>beta-glucuronidase</subject><subject>Biological and medical sciences</subject><subject>Cell physiology</subject><subject>CHX transporters</subject><subject>Conserved Sequence</subject><subject>cortex</subject><subject>DNA Primers</subject><subject>epidermis (plant)</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression regulation</subject><subject>genetic complementation</subject><subject>Genetics</subject><subject>Glucuronidase - genetics</subject><subject>Homeostasis</subject><subject>hydroponics</subject><subject>K+ transport</subject><subject>K+‐starvation</subject><subject>knockout mutants</subject><subject>Life Sciences</subject><subject>Molecular Sequence Data</subject><subject>Na+,K+/H+ antiporters</subject><subject>nucleotide sequences</subject><subject>phenotypic variation</subject><subject>plant morphology</subject><subject>Plant physiology and development</subject><subject>plant proteins</subject><subject>Plants genetics</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>Plasma membrane and permeation</subject><subject>potassium</subject><subject>Potassium - metabolism</subject><subject>reporter genes</subject><subject>roots</subject><subject>Saccharomyces cerevisiae</subject><subject>salt stress</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>signal transduction</subject><subject>sodium, potassium-hydrogen antiporters</subject><subject>Sodium-Hydrogen Exchangers - chemistry</subject><subject>Sodium-Hydrogen Exchangers - genetics</subject><subject>Sodium-Hydrogen Exchangers - metabolism</subject><subject>tissue distribution</subject><subject>transgenic plants</subject><issn>0960-7412</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFuEzEQhlcIRNPCK4AvIKEm6dhe29kDhygCAkQCiVbqzZp1vImj3XVqb2jCI_DUeJtAr_hia_z9M__ozzJCYUzTudqMKZdixCnfjxlAPgZGlRrvn2SDvx-3T7MBFBJGKqfsLDuPcQNAFZf58-yMCp5TydQg-z1bY0DT2eB-Yed8S3xFpt1sfkvVkCBpbFPa0Be7tSXmAbmaXxK7N2tsVzbEIUkwqbBx9WFIquAbMg1YuqXfRheTDGuHLZK4W61s7GJqGnxtiWvJ10uy9o31scOEvsieVVhH-_J0X2Q3Hz9cz-ajxbdPn2fTxcgIWajRJIdk3UhjSi5pzquCFSWWdrmE5cQAV2oiZUFxIoBDrkQpkEElLBjBqsogv8jeHfsmZ3obXIPhoD06PZ8udF8DJhWnQv2kiX17ZLfB3-2Sfd24aGxdY2v9Lmopkx2WiwROjqAJPsZgq3-dKeg-M73RfTS6j0b3memHzPQ-SV-dZuzKxi4fhaeQEvDmBGA0WFcBW-PiIycpABMsce-P3L2r7eG_Dejr71_6V9K_Puor9BpXIc24-cGAcoBCsLQm_wPN5rnr</recordid><startdate>200409</startdate><enddate>200409</enddate><creator>Cellier, F</creator><creator>Conejero, G</creator><creator>Ricaud, L</creator><creator>Luu, D.T</creator><creator>Lepetit, M</creator><creator>Gosti, F</creator><creator>Casse, F</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><general>Wiley</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-0372-5985</orcidid><orcidid>https://orcid.org/0000-0003-0559-5317</orcidid></search><sort><creationdate>200409</creationdate><title>Characterization of AtCHX17, a member of the cation/H+ exchangers, CHX family, from Arabidopsis thaliana suggests a role in K+ homeostasis</title><author>Cellier, F ; Conejero, G ; Ricaud, L ; Luu, D.T ; Lepetit, M ; Gosti, F ; Casse, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5697-840162c6ccb36143f929babedd0d8c037786691a85030475b5a20f5e0c52ffca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>abscisic acid</topic><topic>alleles</topic><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>antiporters</topic><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - chemistry</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Arabidopsis thaliana</topic><topic>AtCHX17 gene</topic><topic>Base Sequence</topic><topic>beta-glucuronidase</topic><topic>Biological and medical sciences</topic><topic>Cell physiology</topic><topic>CHX transporters</topic><topic>Conserved Sequence</topic><topic>cortex</topic><topic>DNA Primers</topic><topic>epidermis (plant)</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression regulation</topic><topic>genetic complementation</topic><topic>Genetics</topic><topic>Glucuronidase - genetics</topic><topic>Homeostasis</topic><topic>hydroponics</topic><topic>K+ transport</topic><topic>K+‐starvation</topic><topic>knockout mutants</topic><topic>Life Sciences</topic><topic>Molecular Sequence Data</topic><topic>Na+,K+/H+ antiporters</topic><topic>nucleotide sequences</topic><topic>phenotypic variation</topic><topic>plant morphology</topic><topic>Plant physiology and development</topic><topic>plant proteins</topic><topic>Plants genetics</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>Plasma membrane and permeation</topic><topic>potassium</topic><topic>Potassium - metabolism</topic><topic>reporter genes</topic><topic>roots</topic><topic>Saccharomyces cerevisiae</topic><topic>salt stress</topic><topic>Sequence Alignment</topic><topic>Sequence Homology, Amino Acid</topic><topic>signal transduction</topic><topic>sodium, potassium-hydrogen antiporters</topic><topic>Sodium-Hydrogen Exchangers - chemistry</topic><topic>Sodium-Hydrogen Exchangers - genetics</topic><topic>Sodium-Hydrogen Exchangers - metabolism</topic><topic>tissue distribution</topic><topic>transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cellier, F</creatorcontrib><creatorcontrib>Conejero, G</creatorcontrib><creatorcontrib>Ricaud, L</creatorcontrib><creatorcontrib>Luu, D.T</creatorcontrib><creatorcontrib>Lepetit, M</creatorcontrib><creatorcontrib>Gosti, F</creatorcontrib><creatorcontrib>Casse, F</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>The Plant journal : for cell and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cellier, F</au><au>Conejero, G</au><au>Ricaud, L</au><au>Luu, D.T</au><au>Lepetit, M</au><au>Gosti, F</au><au>Casse, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of AtCHX17, a member of the cation/H+ exchangers, CHX family, from Arabidopsis thaliana suggests a role in K+ homeostasis</atitle><jtitle>The Plant journal : for cell and molecular biology</jtitle><addtitle>Plant J</addtitle><date>2004-09</date><risdate>2004</risdate><volume>39</volume><issue>6</issue><spage>834</spage><epage>846</epage><pages>834-846</pages><issn>0960-7412</issn><eissn>1365-313X</eissn><abstract>The Arabidopsis genome contains many sequences annotated as encoding H+-coupled cotransporters. Among those are the members of the cation:proton antiporter-2 (CPA2) family (or CHX family), predicted to encode Na+,K+/H+ antiporters. AtCHX17, a member of the CPA2 family, was selected for expression studies, and phenotypic analysis of knockout mutants was performed. AtCHX17 expression was only detected in roots. The gene was strongly induced by salt stress, potassium starvation, abscisic acid (ABA) and external acidic pH. Using the β-glucuronidase reporter gene strategy and in situ RT-PCR experiments, we have found that AtCHX17 was expressed preferentially in epidermal and cortical cells of the mature root zones. Knockout mutants accumulated less K+ in roots in response to salt stress and potassium starvation compared with the wild type. These data support the hypothesis that AtCHX17 is involved in K+ acquisition and homeostasis.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>15341627</pmid><doi>10.1111/j.1365-313x.2004.02177.x</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-0372-5985</orcidid><orcidid>https://orcid.org/0000-0003-0559-5317</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | abscisic acid alleles Amino Acid Sequence amino acid sequences antiporters Arabidopsis Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - chemistry Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis thaliana AtCHX17 gene Base Sequence beta-glucuronidase Biological and medical sciences Cell physiology CHX transporters Conserved Sequence cortex DNA Primers epidermis (plant) Fundamental and applied biological sciences. Psychology gene expression regulation genetic complementation Genetics Glucuronidase - genetics Homeostasis hydroponics K+ transport K+‐starvation knockout mutants Life Sciences Molecular Sequence Data Na+,K+/H+ antiporters nucleotide sequences phenotypic variation plant morphology Plant physiology and development plant proteins Plants genetics Plants, Genetically Modified - metabolism Plasma membrane and permeation potassium Potassium - metabolism reporter genes roots Saccharomyces cerevisiae salt stress Sequence Alignment Sequence Homology, Amino Acid signal transduction sodium, potassium-hydrogen antiporters Sodium-Hydrogen Exchangers - chemistry Sodium-Hydrogen Exchangers - genetics Sodium-Hydrogen Exchangers - metabolism tissue distribution transgenic plants |
| title | Characterization of AtCHX17, a member of the cation/H+ exchangers, CHX family, from Arabidopsis thaliana suggests a role in K+ homeostasis |
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