Comparative effects of auxin transport inhibitors on rhizogenesis and mycorrhizal establishment of spruce seedlings inoculated with Laccaria bicolor
We compared the effects of two auxin transport inhibitors (2,3,5-triiodobenzoic acid (TIBA) and 1-N-naphthylphthalamic acid (NPA)) on rhizogenesis and mycorrhizal establishment of Picea abies L. (Karst.) seedlings inoculated with Laccaria bicolor S238N (Maire) Orton. Inoculation of seedlings with L....
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| Veröffentlicht in: | Tree physiology 2003-08, Vol.23 (11), p.785-791 |
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| creator | Rincon, A Priha, O Sotta, B Bonnet, M Le Tacon, F |
| description | We compared the effects of two auxin transport inhibitors (2,3,5-triiodobenzoic acid (TIBA) and 1-N-naphthylphthalamic acid (NPA)) on rhizogenesis and mycorrhizal establishment of Picea abies L. (Karst.) seedlings inoculated with Laccaria bicolor S238N (Maire) Orton. Inoculation of seedlings with L. bicolor under in vitro conditions strongly increased host root and shoot growth. Although TIBA had no effect on taproot growth, NPA decreased taproot growth and deformed the root apex into a globular shape in both non-inoculated seedlings and seedlings inoculated with L. bicolor. Inoculation with L. bicolor strongly increased lateral rhizogenesis of the seedlings, and application of 100 micromolar indole-3-acetic acid (IAA) partially reproduced this effect. Although TIBA completely inhibited the stimulatory effect of L. bicolor on lateral root formation, NPA inhibited it only partially. Both TIBA and NPA counteracted the effect of exogenous IAA on lateral rhizogenesis. Inoculation with L. bicolor significantly increased shoot growth and seedling dry biomass, whereas application of exogenous IAA had no effect on either parameter. There was no effect of NPA on shoot growth and biomass production. The presence of TIBA completely prevented the development of ectomycorrhizal structures (mantle and Hartig net). In the presence of NPA, the number of seedlings colonized by the fungus was reduced and the degree of development of ectomycorrhizal structures was variable, but not completely prevented. In medium lacking tryptophan, neither TIBA nor NPA inhibited the release of IAA produced by L. bicolor in pure culture. When 100 micromolar tryptophan was added to the medium, TIBA significantly increased the amount of IAA released by the fungus, whereas NPA had no significant effect. We conclude that fungal IAA plays an important role in plant rhizogenesis and in the establishment of ectomycorrhizal symbiosis. |
| doi_str_mv | 10.1093/treephys/23.11.785 |
| format | Article |
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(Karst.) seedlings inoculated with Laccaria bicolor S238N (Maire) Orton. Inoculation of seedlings with L. bicolor under in vitro conditions strongly increased host root and shoot growth. Although TIBA had no effect on taproot growth, NPA decreased taproot growth and deformed the root apex into a globular shape in both non-inoculated seedlings and seedlings inoculated with L. bicolor. Inoculation with L. bicolor strongly increased lateral rhizogenesis of the seedlings, and application of 100 micromolar indole-3-acetic acid (IAA) partially reproduced this effect. Although TIBA completely inhibited the stimulatory effect of L. bicolor on lateral root formation, NPA inhibited it only partially. Both TIBA and NPA counteracted the effect of exogenous IAA on lateral rhizogenesis. Inoculation with L. bicolor significantly increased shoot growth and seedling dry biomass, whereas application of exogenous IAA had no effect on either parameter. There was no effect of NPA on shoot growth and biomass production. The presence of TIBA completely prevented the development of ectomycorrhizal structures (mantle and Hartig net). In the presence of NPA, the number of seedlings colonized by the fungus was reduced and the degree of development of ectomycorrhizal structures was variable, but not completely prevented. In medium lacking tryptophan, neither TIBA nor NPA inhibited the release of IAA produced by L. bicolor in pure culture. When 100 micromolar tryptophan was added to the medium, TIBA significantly increased the amount of IAA released by the fungus, whereas NPA had no significant effect. We conclude that fungal IAA plays an important role in plant rhizogenesis and in the establishment of ectomycorrhizal symbiosis.</description><identifier>ISSN: 0829-318X</identifier><identifier>EISSN: 1758-4469</identifier><identifier>DOI: 10.1093/treephys/23.11.785</identifier><identifier>PMID: 12839732</identifier><language>eng</language><publisher>Canada: Oxford University Press (OUP)</publisher><subject>1-n-naphthylphthalamic acid ; 2,3,5-triiodobenzoic acid ; Agaricales - physiology ; Agricultural sciences ; auxins ; developmental stages ; dry matter accumulation ; ectomycorrhizae ; hormone antagonists ; host plants ; in vitro culture ; indole acetic acid ; Indoleacetic Acids - antagonists & inhibitors ; Laccaria bicolor ; Life Sciences ; microbial colonization ; Mycorrhizae - physiology ; mycorrhizal fungi ; nutrient transport ; Phthalimides - pharmacology ; Picea - microbiology ; Picea - physiology ; Picea abies ; plant anatomy ; plant development ; plant hormones ; Plant Roots - microbiology ; Plant Roots - physiology ; root growth ; seedling growth ; Seedlings - microbiology ; Seedlings - physiology ; shoots ; Silviculture, forestry ; Trees - microbiology ; Trees - physiology ; Triiodobenzoic Acids - pharmacology</subject><ispartof>Tree physiology, 2003-08, Vol.23 (11), p.785-791</ispartof><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-e41fe11d6044cf84e6420357ab89ce224f4e6408c172229860136d5e1c5bbc383</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12839732$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02670847$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Rincon, A</creatorcontrib><creatorcontrib>Priha, O</creatorcontrib><creatorcontrib>Sotta, B</creatorcontrib><creatorcontrib>Bonnet, M</creatorcontrib><creatorcontrib>Le Tacon, F</creatorcontrib><title>Comparative effects of auxin transport inhibitors on rhizogenesis and mycorrhizal establishment of spruce seedlings inoculated with Laccaria bicolor</title><title>Tree physiology</title><addtitle>Tree Physiol</addtitle><description>We compared the effects of two auxin transport inhibitors (2,3,5-triiodobenzoic acid (TIBA) and 1-N-naphthylphthalamic acid (NPA)) on rhizogenesis and mycorrhizal establishment of Picea abies L. (Karst.) seedlings inoculated with Laccaria bicolor S238N (Maire) Orton. Inoculation of seedlings with L. bicolor under in vitro conditions strongly increased host root and shoot growth. Although TIBA had no effect on taproot growth, NPA decreased taproot growth and deformed the root apex into a globular shape in both non-inoculated seedlings and seedlings inoculated with L. bicolor. Inoculation with L. bicolor strongly increased lateral rhizogenesis of the seedlings, and application of 100 micromolar indole-3-acetic acid (IAA) partially reproduced this effect. Although TIBA completely inhibited the stimulatory effect of L. bicolor on lateral root formation, NPA inhibited it only partially. Both TIBA and NPA counteracted the effect of exogenous IAA on lateral rhizogenesis. Inoculation with L. bicolor significantly increased shoot growth and seedling dry biomass, whereas application of exogenous IAA had no effect on either parameter. There was no effect of NPA on shoot growth and biomass production. The presence of TIBA completely prevented the development of ectomycorrhizal structures (mantle and Hartig net). In the presence of NPA, the number of seedlings colonized by the fungus was reduced and the degree of development of ectomycorrhizal structures was variable, but not completely prevented. In medium lacking tryptophan, neither TIBA nor NPA inhibited the release of IAA produced by L. bicolor in pure culture. When 100 micromolar tryptophan was added to the medium, TIBA significantly increased the amount of IAA released by the fungus, whereas NPA had no significant effect. We conclude that fungal IAA plays an important role in plant rhizogenesis and in the establishment of ectomycorrhizal symbiosis.</description><subject>1-n-naphthylphthalamic acid</subject><subject>2,3,5-triiodobenzoic acid</subject><subject>Agaricales - physiology</subject><subject>Agricultural sciences</subject><subject>auxins</subject><subject>developmental stages</subject><subject>dry matter accumulation</subject><subject>ectomycorrhizae</subject><subject>hormone antagonists</subject><subject>host plants</subject><subject>in vitro culture</subject><subject>indole acetic acid</subject><subject>Indoleacetic Acids - antagonists & inhibitors</subject><subject>Laccaria bicolor</subject><subject>Life Sciences</subject><subject>microbial colonization</subject><subject>Mycorrhizae - physiology</subject><subject>mycorrhizal fungi</subject><subject>nutrient transport</subject><subject>Phthalimides - pharmacology</subject><subject>Picea - microbiology</subject><subject>Picea - physiology</subject><subject>Picea abies</subject><subject>plant anatomy</subject><subject>plant development</subject><subject>plant hormones</subject><subject>Plant Roots - microbiology</subject><subject>Plant Roots - physiology</subject><subject>root growth</subject><subject>seedling growth</subject><subject>Seedlings - microbiology</subject><subject>Seedlings - physiology</subject><subject>shoots</subject><subject>Silviculture, forestry</subject><subject>Trees - microbiology</subject><subject>Trees - physiology</subject><subject>Triiodobenzoic Acids - pharmacology</subject><issn>0829-318X</issn><issn>1758-4469</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkcGO0zAQhi0EYsvCC3AAn5A4tOuxncQ5ripgkSpxgJW4WY4zaYySONjOQnkOHhhHLXCy9PubbzT6CXkJbAesFjcpIM79Kd5wsQPYVap4RDZQFWorZVk_JhumeL0VoL5ekWcxfmMMCqXqp-QKuBJ1JfiG_N77cTbBJPeAFLsObYrUd9QsP91EUzBTnH1I1E29a1zyIf9ONPTulz_ihNFFaqaWjifrw5qagWJMphlc7Eec0uqKc1gs0ojYDm46xizzdhlMwpb-cKmnB2OtCc7Qxlk_-PCcPOnMEPHF5b0m9-_ffdnfbQ-fPnzc3x62VjJIW5TQIUBbMiltpySWkjNRVKZRtUXOZbdGTFmoOOe1KhmIsi0QbNE0VihxTd6evb0Z9BzcaMJJe-P03e1BrxnjZcWUrB4gs2_O7Bz89yXfqEcXLQ6DmdAvUVdC5iVqBfkZtMHHGLD7Zwam19703940FxpA597y0KuLfWlGbP-PXIrKwOsz0BmvzTG4qO8_83wQy8aCsUL8Aa7AosI</recordid><startdate>20030801</startdate><enddate>20030801</enddate><creator>Rincon, A</creator><creator>Priha, O</creator><creator>Sotta, B</creator><creator>Bonnet, M</creator><creator>Le Tacon, F</creator><general>Oxford University Press (OUP)</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>20030801</creationdate><title>Comparative effects of auxin transport inhibitors on rhizogenesis and mycorrhizal establishment of spruce seedlings inoculated with Laccaria bicolor</title><author>Rincon, A ; Priha, O ; Sotta, B ; Bonnet, M ; Le Tacon, F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-e41fe11d6044cf84e6420357ab89ce224f4e6408c172229860136d5e1c5bbc383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>1-n-naphthylphthalamic acid</topic><topic>2,3,5-triiodobenzoic acid</topic><topic>Agaricales - physiology</topic><topic>Agricultural sciences</topic><topic>auxins</topic><topic>developmental stages</topic><topic>dry matter accumulation</topic><topic>ectomycorrhizae</topic><topic>hormone antagonists</topic><topic>host plants</topic><topic>in vitro culture</topic><topic>indole acetic acid</topic><topic>Indoleacetic Acids - antagonists & inhibitors</topic><topic>Laccaria bicolor</topic><topic>Life Sciences</topic><topic>microbial colonization</topic><topic>Mycorrhizae - physiology</topic><topic>mycorrhizal fungi</topic><topic>nutrient transport</topic><topic>Phthalimides - pharmacology</topic><topic>Picea - microbiology</topic><topic>Picea - physiology</topic><topic>Picea abies</topic><topic>plant anatomy</topic><topic>plant development</topic><topic>plant hormones</topic><topic>Plant Roots - microbiology</topic><topic>Plant Roots - physiology</topic><topic>root growth</topic><topic>seedling growth</topic><topic>Seedlings - microbiology</topic><topic>Seedlings - physiology</topic><topic>shoots</topic><topic>Silviculture, forestry</topic><topic>Trees - microbiology</topic><topic>Trees - physiology</topic><topic>Triiodobenzoic Acids - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rincon, A</creatorcontrib><creatorcontrib>Priha, O</creatorcontrib><creatorcontrib>Sotta, B</creatorcontrib><creatorcontrib>Bonnet, M</creatorcontrib><creatorcontrib>Le Tacon, F</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Tree physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rincon, A</au><au>Priha, O</au><au>Sotta, B</au><au>Bonnet, M</au><au>Le Tacon, F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative effects of auxin transport inhibitors on rhizogenesis and mycorrhizal establishment of spruce seedlings inoculated with Laccaria bicolor</atitle><jtitle>Tree physiology</jtitle><addtitle>Tree Physiol</addtitle><date>2003-08-01</date><risdate>2003</risdate><volume>23</volume><issue>11</issue><spage>785</spage><epage>791</epage><pages>785-791</pages><issn>0829-318X</issn><eissn>1758-4469</eissn><abstract>We compared the effects of two auxin transport inhibitors (2,3,5-triiodobenzoic acid (TIBA) and 1-N-naphthylphthalamic acid (NPA)) on rhizogenesis and mycorrhizal establishment of Picea abies L. (Karst.) seedlings inoculated with Laccaria bicolor S238N (Maire) Orton. Inoculation of seedlings with L. bicolor under in vitro conditions strongly increased host root and shoot growth. Although TIBA had no effect on taproot growth, NPA decreased taproot growth and deformed the root apex into a globular shape in both non-inoculated seedlings and seedlings inoculated with L. bicolor. Inoculation with L. bicolor strongly increased lateral rhizogenesis of the seedlings, and application of 100 micromolar indole-3-acetic acid (IAA) partially reproduced this effect. Although TIBA completely inhibited the stimulatory effect of L. bicolor on lateral root formation, NPA inhibited it only partially. Both TIBA and NPA counteracted the effect of exogenous IAA on lateral rhizogenesis. Inoculation with L. bicolor significantly increased shoot growth and seedling dry biomass, whereas application of exogenous IAA had no effect on either parameter. There was no effect of NPA on shoot growth and biomass production. The presence of TIBA completely prevented the development of ectomycorrhizal structures (mantle and Hartig net). In the presence of NPA, the number of seedlings colonized by the fungus was reduced and the degree of development of ectomycorrhizal structures was variable, but not completely prevented. In medium lacking tryptophan, neither TIBA nor NPA inhibited the release of IAA produced by L. bicolor in pure culture. When 100 micromolar tryptophan was added to the medium, TIBA significantly increased the amount of IAA released by the fungus, whereas NPA had no significant effect. We conclude that fungal IAA plays an important role in plant rhizogenesis and in the establishment of ectomycorrhizal symbiosis.</abstract><cop>Canada</cop><pub>Oxford University Press (OUP)</pub><pmid>12839732</pmid><doi>10.1093/treephys/23.11.785</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE |
| subjects | 1-n-naphthylphthalamic acid 2,3,5-triiodobenzoic acid Agaricales - physiology Agricultural sciences auxins developmental stages dry matter accumulation ectomycorrhizae hormone antagonists host plants in vitro culture indole acetic acid Indoleacetic Acids - antagonists & inhibitors Laccaria bicolor Life Sciences microbial colonization Mycorrhizae - physiology mycorrhizal fungi nutrient transport Phthalimides - pharmacology Picea - microbiology Picea - physiology Picea abies plant anatomy plant development plant hormones Plant Roots - microbiology Plant Roots - physiology root growth seedling growth Seedlings - microbiology Seedlings - physiology shoots Silviculture, forestry Trees - microbiology Trees - physiology Triiodobenzoic Acids - pharmacology |
| title | Comparative effects of auxin transport inhibitors on rhizogenesis and mycorrhizal establishment of spruce seedlings inoculated with Laccaria bicolor |
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