Single-nucleotide polymorphisms in two Marek's disease virus genes (Meq and gD): application to a retrospective molecular epidemiology study (1982-1999) in France
Laboratoire de Virologie et Barrière d'Espèces, UR 086, INRA, Centre de recherches de Tours, 37380 Nouzilly, France Correspondence S. Laurent slaurent{at}tours.inra.fr Marek's disease virus (MDV) is a herpesvirus that causes a lymphoproliferative disease in chickens. Vaccines against MDV a...
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| Veröffentlicht in: | Journal of general virology 2004-06, Vol.85 (6), p.1387-1392 |
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| creator | Laurent, S Esnault, E Rasschaert, D |
| description | Laboratoire de Virologie et Barrière d'Espèces, UR 086, INRA, Centre de recherches de Tours, 37380 Nouzilly, France
Correspondence S. Laurent slaurent{at}tours.inra.fr
Marek's disease virus (MDV) is a herpesvirus that causes a lymphoproliferative disease in chickens. Vaccines against MDV are available, but the virus is gradually becoming more virulent. A molecular epidemiology study of MDV was carried out by assessing nucleotide variation in two different genes, Meq and gD , in 68 French field isolates circulating from 1982 to 1999, compared with reference strains. Viral DNA was amplified by nested PCR and sequenced directly. Comparison of the nucleotide sequences revealed a high nucleotide sequence identity (98 %). Single-nucleotide polymorphisms were identified, leading to the identification of three gene alleles for gD and six for Meq . Nine combinations of alleles were identified. A majority of French isolates (60·5 %) clustered in the C1 type, which has been present for over 17 years. Waves of non-C1-type isolates appeared when vaccine efficacy decreased. Furthermore, specific discriminating sequences were obtained for the CVI-988 vaccine strain. |
| doi_str_mv | 10.1099/vir.0.79782-0 |
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Correspondence S. Laurent slaurent{at}tours.inra.fr
Marek's disease virus (MDV) is a herpesvirus that causes a lymphoproliferative disease in chickens. Vaccines against MDV are available, but the virus is gradually becoming more virulent. A molecular epidemiology study of MDV was carried out by assessing nucleotide variation in two different genes, Meq and gD , in 68 French field isolates circulating from 1982 to 1999, compared with reference strains. Viral DNA was amplified by nested PCR and sequenced directly. Comparison of the nucleotide sequences revealed a high nucleotide sequence identity (98 %). Single-nucleotide polymorphisms were identified, leading to the identification of three gene alleles for gD and six for Meq . Nine combinations of alleles were identified. A majority of French isolates (60·5 %) clustered in the C1 type, which has been present for over 17 years. Waves of non-C1-type isolates appeared when vaccine efficacy decreased. Furthermore, specific discriminating sequences were obtained for the CVI-988 vaccine strain.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/vir.0.79782-0</identifier><identifier>PMID: 15166420</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Animals ; Biological and medical sciences ; Epidemiology ; France - epidemiology ; Fundamental and applied biological sciences. Psychology ; Herpesvirus ; Herpesvirus 2, Gallid - genetics ; Life Sciences ; Marek Disease - epidemiology ; Marek's disease herpesvirus ; Microbiology ; Microbiology and Parasitology ; Miscellaneous ; Molecular Epidemiology ; Oncogene Proteins, Viral - genetics ; Polymorphism, Single Nucleotide ; Retrospective Studies ; Time Factors ; Vaccination ; Virology</subject><ispartof>Journal of general virology, 2004-06, Vol.85 (6), p.1387-1392</ispartof><rights>2004 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c480t-3350d179af023151da0d824d2cf1a3993572c87a73b4b3e4c97b3a425b4d72663</citedby><cites>FETCH-LOGICAL-c480t-3350d179af023151da0d824d2cf1a3993572c87a73b4b3e4c97b3a425b4d72663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,777,781,882,3733,3734,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15841188$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15166420$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02670686$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Laurent, S</creatorcontrib><creatorcontrib>Esnault, E</creatorcontrib><creatorcontrib>Rasschaert, D</creatorcontrib><title>Single-nucleotide polymorphisms in two Marek's disease virus genes (Meq and gD): application to a retrospective molecular epidemiology study (1982-1999) in France</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>Laboratoire de Virologie et Barrière d'Espèces, UR 086, INRA, Centre de recherches de Tours, 37380 Nouzilly, France
Correspondence S. Laurent slaurent{at}tours.inra.fr
Marek's disease virus (MDV) is a herpesvirus that causes a lymphoproliferative disease in chickens. Vaccines against MDV are available, but the virus is gradually becoming more virulent. A molecular epidemiology study of MDV was carried out by assessing nucleotide variation in two different genes, Meq and gD , in 68 French field isolates circulating from 1982 to 1999, compared with reference strains. Viral DNA was amplified by nested PCR and sequenced directly. Comparison of the nucleotide sequences revealed a high nucleotide sequence identity (98 %). Single-nucleotide polymorphisms were identified, leading to the identification of three gene alleles for gD and six for Meq . Nine combinations of alleles were identified. A majority of French isolates (60·5 %) clustered in the C1 type, which has been present for over 17 years. Waves of non-C1-type isolates appeared when vaccine efficacy decreased. Furthermore, specific discriminating sequences were obtained for the CVI-988 vaccine strain.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Epidemiology</subject><subject>France - epidemiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Herpesvirus</subject><subject>Herpesvirus 2, Gallid - genetics</subject><subject>Life Sciences</subject><subject>Marek Disease - epidemiology</subject><subject>Marek's disease herpesvirus</subject><subject>Microbiology</subject><subject>Microbiology and Parasitology</subject><subject>Miscellaneous</subject><subject>Molecular Epidemiology</subject><subject>Oncogene Proteins, Viral - genetics</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Retrospective Studies</subject><subject>Time Factors</subject><subject>Vaccination</subject><subject>Virology</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhSMEokNhyRa8AWYWGa5_EtvsqkIp0lQsSteWkzgZgxOndjLVvA5PiocZATtWluzP99xzTpa9xLDGIOX7nQ1rWHPJBcnhUbbArCxykl4eZwsAQnJMMT_LnsX4HQAzVvCn2RkucFkyAovs560dOmfyYa6d8ZNtDBq92_c-jFsb-4jsgKYHj250MD_eRdTYaHQ0KMnOEXVmMBEtb8w90kODuo-rD0iPo7O1nqxPPz3SKJgp-DiaerI7g3rvTD07HZAZk1pvvfPdHsVpbvZoiWWygaWUq4PwVdBDbZ5nT1rtonlxOs-zu6tP3y6v883Xz18uLzZ5zQRMOaUFNJhL3QKhyWCjoRGENaRusaZS0oKTWnDNacUqalgteUU1I0XFGk7Kkp5nq-PcrXZqDLbXYa-8tur6YqMOd0BKDqUodzixb4_sGPz9bOKkehtr45wejJ-j4lhyYLT4L4gFlJhgksD8CNYprBhM-2cFDOrQtEqRK1C_m1aQ-FenwXPVm-Yvfao2AW9OgI61du0hSxv_4QTDWIjELU-2bbd9sMGo1Gpv0xqV9QdRUahSYSp4Ql8f0VZ7pbuQxt3dEsAUMJCCCEp_ARdmx4E</recordid><startdate>20040601</startdate><enddate>20040601</enddate><creator>Laurent, S</creator><creator>Esnault, E</creator><creator>Rasschaert, D</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><general>Microbiology Society</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>20040601</creationdate><title>Single-nucleotide polymorphisms in two Marek's disease virus genes (Meq and gD): application to a retrospective molecular epidemiology study (1982-1999) in France</title><author>Laurent, S ; Esnault, E ; Rasschaert, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c480t-3350d179af023151da0d824d2cf1a3993572c87a73b4b3e4c97b3a425b4d72663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Epidemiology</topic><topic>France - epidemiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Herpesvirus</topic><topic>Herpesvirus 2, Gallid - genetics</topic><topic>Life Sciences</topic><topic>Marek Disease - epidemiology</topic><topic>Marek's disease herpesvirus</topic><topic>Microbiology</topic><topic>Microbiology and Parasitology</topic><topic>Miscellaneous</topic><topic>Molecular Epidemiology</topic><topic>Oncogene Proteins, Viral - genetics</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Retrospective Studies</topic><topic>Time Factors</topic><topic>Vaccination</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Laurent, S</creatorcontrib><creatorcontrib>Esnault, E</creatorcontrib><creatorcontrib>Rasschaert, D</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Laurent, S</au><au>Esnault, E</au><au>Rasschaert, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Single-nucleotide polymorphisms in two Marek's disease virus genes (Meq and gD): application to a retrospective molecular epidemiology study (1982-1999) in France</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>2004-06-01</date><risdate>2004</risdate><volume>85</volume><issue>6</issue><spage>1387</spage><epage>1392</epage><pages>1387-1392</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>Laboratoire de Virologie et Barrière d'Espèces, UR 086, INRA, Centre de recherches de Tours, 37380 Nouzilly, France
Correspondence S. Laurent slaurent{at}tours.inra.fr
Marek's disease virus (MDV) is a herpesvirus that causes a lymphoproliferative disease in chickens. Vaccines against MDV are available, but the virus is gradually becoming more virulent. A molecular epidemiology study of MDV was carried out by assessing nucleotide variation in two different genes, Meq and gD , in 68 French field isolates circulating from 1982 to 1999, compared with reference strains. Viral DNA was amplified by nested PCR and sequenced directly. Comparison of the nucleotide sequences revealed a high nucleotide sequence identity (98 %). Single-nucleotide polymorphisms were identified, leading to the identification of three gene alleles for gD and six for Meq . Nine combinations of alleles were identified. A majority of French isolates (60·5 %) clustered in the C1 type, which has been present for over 17 years. Waves of non-C1-type isolates appeared when vaccine efficacy decreased. Furthermore, specific discriminating sequences were obtained for the CVI-988 vaccine strain.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>15166420</pmid><doi>10.1099/vir.0.79782-0</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biological and medical sciences Epidemiology France - epidemiology Fundamental and applied biological sciences. Psychology Herpesvirus Herpesvirus 2, Gallid - genetics Life Sciences Marek Disease - epidemiology Marek's disease herpesvirus Microbiology Microbiology and Parasitology Miscellaneous Molecular Epidemiology Oncogene Proteins, Viral - genetics Polymorphism, Single Nucleotide Retrospective Studies Time Factors Vaccination Virology |
| title | Single-nucleotide polymorphisms in two Marek's disease virus genes (Meq and gD): application to a retrospective molecular epidemiology study (1982-1999) in France |
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