Prevalence and distribution of the insertion element ISMag1 in Mycoplasma agalactiae
In characterising Mycoplasma agalactiae strains from various European countries and from Africa, a new insertion sequence (IS), ISMag1, which is related to IS of the family of IS30 insertion elements, has been identified by DNA sequence analysis and Southern blot hybridisation. ISMag1 has a size of...
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| Veröffentlicht in: | Veterinary microbiology 2003-03, Vol.92 (1-2), p.37-48 |
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| creator | PILO, Paola FLEURY, Bénédicte MARENDA, Marc FREY, Joachim VILEI, Edy M |
| description | In characterising Mycoplasma agalactiae strains from various European countries and from Africa, a new insertion sequence (IS), ISMag1, which is related to IS of the family of IS30 insertion elements, has been identified by DNA sequence analysis and Southern blot hybridisation. ISMag1 has a size of 1515bp, and contains inverted repeats of 3bp and a gene encoding the putative transposase on a single open reading frame. ISMag1 is present only in the rarely isolated serotypes E, F, G and H of M. agalactiae, where it is found in 1 to approximately 30 copies. The different patterns obtained by hybridisation of a labelled probe of ISMag1 to genomic DNA cut with various restriction enzymes correlate to some extent to the different serotypes and to variations of the nucleotide sequences of the uvrC genes of the different strains. Based on uvrC sequences, the strains of M. agalactiae carrying ISMag1 form a cluster, separate from the other strains. IS patterns obtained with ISMag1 allow a fine subtyping of the serotypes E, F, G and H of M. agalactiae for epidemiological studies. The potential role of ISMag1 and of its copy numbers on virulence and persistence of the respective strains requests further studies. |
| doi_str_mv | 10.1016/S0378-1135(02)00311-5 |
| format | Article |
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ISMag1 has a size of 1515bp, and contains inverted repeats of 3bp and a gene encoding the putative transposase on a single open reading frame. ISMag1 is present only in the rarely isolated serotypes E, F, G and H of M. agalactiae, where it is found in 1 to approximately 30 copies. The different patterns obtained by hybridisation of a labelled probe of ISMag1 to genomic DNA cut with various restriction enzymes correlate to some extent to the different serotypes and to variations of the nucleotide sequences of the uvrC genes of the different strains. Based on uvrC sequences, the strains of M. agalactiae carrying ISMag1 form a cluster, separate from the other strains. IS patterns obtained with ISMag1 allow a fine subtyping of the serotypes E, F, G and H of M. agalactiae for epidemiological studies. 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ISMag1 has a size of 1515bp, and contains inverted repeats of 3bp and a gene encoding the putative transposase on a single open reading frame. ISMag1 is present only in the rarely isolated serotypes E, F, G and H of M. agalactiae, where it is found in 1 to approximately 30 copies. The different patterns obtained by hybridisation of a labelled probe of ISMag1 to genomic DNA cut with various restriction enzymes correlate to some extent to the different serotypes and to variations of the nucleotide sequences of the uvrC genes of the different strains. Based on uvrC sequences, the strains of M. agalactiae carrying ISMag1 form a cluster, separate from the other strains. IS patterns obtained with ISMag1 allow a fine subtyping of the serotypes E, F, G and H of M. agalactiae for epidemiological studies. The potential role of ISMag1 and of its copy numbers on virulence and persistence of the respective strains requests further studies.</description><subject>Amino Acid Sequence</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>DNA Probes - chemistry</subject><subject>DNA Probes - genetics</subject><subject>DNA Transposable Elements - genetics</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>Epidemiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Variation</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Mycoplasma - classification</subject><subject>Mycoplasma - genetics</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction</subject><subject>Sequence Analysis, DNA</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQQC0EokvhJ4ByAdFDYMZJbOdYVUArbQVSy9maOJPWyEkWO1up_x7vh9ojM4eRxm_skZ8Q7xG-IKD6egOVNiVi1XwGeQZQIZbNC7FCo6tSNrV8KVZPyIl4k9IfAKhbBa_FCcraGFDtStz-ivxAgSfHBU190fu0RN9tFz9PxTwUyz0Xfkoc9w0OPPK0FFc313SH-aC4fnTzJlAaqaA7CuQWT_xWvBooJH53rKfi9_dvtxeX5frnj6uL83XpaoClHEg7iaqTpq61I0Ype4eMrXKd6YYm11rnaGrS0BrDhqEHaND1bdXrtjoVZ4d77ynYTfQjxUc7k7eX52u764FUqtWoHjCznw7sJs5_t5wWO_rkOASaeN4mq6VpQaL8L4hG5awhg80BdHFOKfLwtAKC3Tmye0d2JyBvYveObJPnPhwf2HYj989TRykZ-HgEKDkKQ6TJ-fTM5d8yGpvqH870l9Y</recordid><startdate>20030320</startdate><enddate>20030320</enddate><creator>PILO, Paola</creator><creator>FLEURY, Bénédicte</creator><creator>MARENDA, Marc</creator><creator>FREY, Joachim</creator><creator>VILEI, Edy M</creator><general>Elsevier Science</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>20030320</creationdate><title>Prevalence and distribution of the insertion element ISMag1 in Mycoplasma agalactiae</title><author>PILO, Paola ; FLEURY, Bénédicte ; MARENDA, Marc ; FREY, Joachim ; VILEI, Edy M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-fa7c216b28447cae122dc1e196cb8bf596c4777754a70988e8e0d0051cd93d793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Amino Acid Sequence</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>DNA Probes - chemistry</topic><topic>DNA Probes - genetics</topic><topic>DNA Transposable Elements - genetics</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>Epidemiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Variation</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Mycoplasma - classification</topic><topic>Mycoplasma - genetics</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PILO, Paola</creatorcontrib><creatorcontrib>FLEURY, Bénédicte</creatorcontrib><creatorcontrib>MARENDA, Marc</creatorcontrib><creatorcontrib>FREY, Joachim</creatorcontrib><creatorcontrib>VILEI, Edy M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>PILO, Paola</au><au>FLEURY, Bénédicte</au><au>MARENDA, Marc</au><au>FREY, Joachim</au><au>VILEI, Edy M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence and distribution of the insertion element ISMag1 in Mycoplasma agalactiae</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2003-03-20</date><risdate>2003</risdate><volume>92</volume><issue>1-2</issue><spage>37</spage><epage>48</epage><pages>37-48</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>In characterising Mycoplasma agalactiae strains from various European countries and from Africa, a new insertion sequence (IS), ISMag1, which is related to IS of the family of IS30 insertion elements, has been identified by DNA sequence analysis and Southern blot hybridisation. ISMag1 has a size of 1515bp, and contains inverted repeats of 3bp and a gene encoding the putative transposase on a single open reading frame. ISMag1 is present only in the rarely isolated serotypes E, F, G and H of M. agalactiae, where it is found in 1 to approximately 30 copies. The different patterns obtained by hybridisation of a labelled probe of ISMag1 to genomic DNA cut with various restriction enzymes correlate to some extent to the different serotypes and to variations of the nucleotide sequences of the uvrC genes of the different strains. Based on uvrC sequences, the strains of M. agalactiae carrying ISMag1 form a cluster, separate from the other strains. IS patterns obtained with ISMag1 allow a fine subtyping of the serotypes E, F, G and H of M. agalactiae for epidemiological studies. The potential role of ISMag1 and of its copy numbers on virulence and persistence of the respective strains requests further studies.</abstract><cop>Amsterdam</cop><pub>Elsevier Science</pub><pmid>12488069</pmid><doi>10.1016/S0378-1135(02)00311-5</doi><tpages>12</tpages></addata></record> |
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| subjects | Amino Acid Sequence Bacteriology Base Sequence Biological and medical sciences Blotting, Southern DNA Probes - chemistry DNA Probes - genetics DNA Transposable Elements - genetics DNA, Bacterial - chemistry DNA, Bacterial - genetics Epidemiology Fundamental and applied biological sciences. Psychology Genetic Variation Life Sciences Microbiology Molecular Sequence Data Mycoplasma - classification Mycoplasma - genetics Phylogeny Polymerase Chain Reaction Sequence Analysis, DNA |
| title | Prevalence and distribution of the insertion element ISMag1 in Mycoplasma agalactiae |
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