Spontaneous plant regeneration in transformed roots and calli from Tylophora indica: changes in morphological phenotype and tylophorine accumulation associated with transformation by Agrobacterium rhizogenes
We examined the effects of genetic transformation by Agrobacterium rhizogenes on the production of tylophorine, a phenanthroindolizidine alkaloid, in the Indian medicinal plant, Tylophora indica. Transformed roots induced by the bacterium grew in axenic culture and produced shoots or embryogenic cal...
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| creator | Chaudhuri, K.N Ghosh, B Tepfer, D Jha, S |
| description | We examined the effects of genetic transformation by Agrobacterium rhizogenes on the production of tylophorine, a phenanthroindolizidine alkaloid, in the Indian medicinal plant, Tylophora indica. Transformed roots induced by the bacterium grew in axenic culture and produced shoots or embryogenic calli in the absence of hormone treatments. However, hormonal treatment was required to regenerate shoots in root explants of wild type control plants. Transformed plants showed morphological features typically seen in transgenic plants produced by A. rhizogenes, which include, short internodes, small and wrinkled leaves, more branches and numerous plagiotropic roots. Plants regenerated from transformed roots showed increased biomass accumulation (350-510% in the roots and 200-320% in the whole plants) and augmented tylophorine content (20-60%) in the shoots, resulting in a 160-280% increase in tylophorine production in different clones grown in vitro. |
| doi_str_mv | 10.1007/s00299-006-0164-z |
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Transformed roots induced by the bacterium grew in axenic culture and produced shoots or embryogenic calli in the absence of hormone treatments. However, hormonal treatment was required to regenerate shoots in root explants of wild type control plants. Transformed plants showed morphological features typically seen in transgenic plants produced by A. rhizogenes, which include, short internodes, small and wrinkled leaves, more branches and numerous plagiotropic roots. Plants regenerated from transformed roots showed increased biomass accumulation (350-510% in the roots and 200-320% in the whole plants) and augmented tylophorine content (20-60%) in the shoots, resulting in a 160-280% increase in tylophorine production in different clones grown in vitro.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-006-0164-z</identifier><identifier>PMID: 16609889</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Agrobacterium rhizogenes ; Alkaloids - analysis ; Alkaloids - biosynthesis ; Alkaloids - metabolism ; Biological and medical sciences ; Biotechnology ; callus culture ; DNA, Bacterial - metabolism ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; genetic transformation ; Genetics ; indolizidine alkaloids ; Indolizines - analysis ; Indolizines - metabolism ; Life Sciences ; Light ; Medicinal plants ; Methods. Procedures. Technologies ; Miscellaneous ; Phenanthrenes - analysis ; Phenanthrenes - metabolism ; Phenotype ; phenotypic variation ; Plant cells and fungal cells ; plant growth ; plant morphology ; plant regeneration ; Plant Roots - physiology ; Plant Shoots - physiology ; Plants genetics ; Plants, Genetically Modified ; Regeneration - radiation effects ; Rhizobium - metabolism ; Rhizobium rhizogenes ; Roots ; Shoots ; Transformation, Genetic ; Transgenic plants ; Tylophora ; Tylophora - genetics ; Tylophora - growth & development ; Tylophora - physiology ; Tylophora - radiation effects ; Tylophora indica ; tylophorine</subject><ispartof>Plant cell reports, 2006-10, Vol.25 (10), p.1059-1066</ispartof><rights>2007 INIST-CNRS</rights><rights>Springer-Verlag 2006</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-ce392fe8387784e6cb409bf3641bd0e96a072389126edbe4c4aa69acab1401733</citedby><cites>FETCH-LOGICAL-c445t-ce392fe8387784e6cb409bf3641bd0e96a072389126edbe4c4aa69acab1401733</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18172867$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16609889$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02667707$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Chaudhuri, K.N</creatorcontrib><creatorcontrib>Ghosh, B</creatorcontrib><creatorcontrib>Tepfer, D</creatorcontrib><creatorcontrib>Jha, S</creatorcontrib><title>Spontaneous plant regeneration in transformed roots and calli from Tylophora indica: changes in morphological phenotype and tylophorine accumulation associated with transformation by Agrobacterium rhizogenes</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><description>We examined the effects of genetic transformation by Agrobacterium rhizogenes on the production of tylophorine, a phenanthroindolizidine alkaloid, in the Indian medicinal plant, Tylophora indica. Transformed roots induced by the bacterium grew in axenic culture and produced shoots or embryogenic calli in the absence of hormone treatments. However, hormonal treatment was required to regenerate shoots in root explants of wild type control plants. Transformed plants showed morphological features typically seen in transgenic plants produced by A. rhizogenes, which include, short internodes, small and wrinkled leaves, more branches and numerous plagiotropic roots. Plants regenerated from transformed roots showed increased biomass accumulation (350-510% in the roots and 200-320% in the whole plants) and augmented tylophorine content (20-60%) in the shoots, resulting in a 160-280% increase in tylophorine production in different clones grown in vitro.</description><subject>Agrobacterium rhizogenes</subject><subject>Alkaloids - analysis</subject><subject>Alkaloids - biosynthesis</subject><subject>Alkaloids - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>callus culture</subject><subject>DNA, Bacterial - metabolism</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genetic transformation</subject><subject>Genetics</subject><subject>indolizidine alkaloids</subject><subject>Indolizines - analysis</subject><subject>Indolizines - metabolism</subject><subject>Life Sciences</subject><subject>Light</subject><subject>Medicinal plants</subject><subject>Methods. Procedures. Technologies</subject><subject>Miscellaneous</subject><subject>Phenanthrenes - analysis</subject><subject>Phenanthrenes - metabolism</subject><subject>Phenotype</subject><subject>phenotypic variation</subject><subject>Plant cells and fungal cells</subject><subject>plant growth</subject><subject>plant morphology</subject><subject>plant regeneration</subject><subject>Plant Roots - physiology</subject><subject>Plant Shoots - physiology</subject><subject>Plants genetics</subject><subject>Plants, Genetically Modified</subject><subject>Regeneration - radiation effects</subject><subject>Rhizobium - metabolism</subject><subject>Rhizobium rhizogenes</subject><subject>Roots</subject><subject>Shoots</subject><subject>Transformation, Genetic</subject><subject>Transgenic plants</subject><subject>Tylophora</subject><subject>Tylophora - genetics</subject><subject>Tylophora - growth & development</subject><subject>Tylophora - physiology</subject><subject>Tylophora - radiation effects</subject><subject>Tylophora indica</subject><subject>tylophorine</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFksFu1DAQhiMEoqXwAFzAQgKJQ2DspHbMbVUBRVqJQ1uJmzXxOhtXiR1sB7T7krwSXrJiJS6cLHu-mX888xfFcwrvKIB4HwGYlCUAL4Hyutw_KM5pXbGSQfXtYXEOgtFSCFqfFU9ivAfIQcEfF2eUc5BNI8-LXzeTdwmd8XMk04AukWC2xpmAyXpHrCMpoIudD6PZkOB9igTdhmgcBku64Edyuxv81PuAmd5YjR-I7tFtTTxkjz7k2OC3OTCQqTfOp91k_tRIx0Tr8l3reZyHRRVj9Npiyoo_bepPLSzhdkdW2-Bb1MkEO48k9HbvD13Hp8WjDodonh3Pi-Lu08fbq-ty_fXzl6vVutR1fZlKbSrJOtNUjRBNbbhua5BtV_GathswkmOeXdVIyrjZtKbWNSKXqLGlNVBRVRfF26Vuj4Oagh0x7JRHq65Xa3V4A8a5ECB-0My-Wdgp-O-ziUmNNmozDMvYFW8axiRU_wUZ5J4EyAy--ge893Nw-cOqAXFJs3idIbpAOvgYg-n-9klBHfyjFv-o7B918I_a55wXx8Jzm_d9yjgaJgOvjwDGvNAuL0bbeOIaKljDReZeLlyHXuE2ZObuhgGtgGZpymT1G2yZ3Ow</recordid><startdate>20061001</startdate><enddate>20061001</enddate><creator>Chaudhuri, K.N</creator><creator>Ghosh, B</creator><creator>Tepfer, D</creator><creator>Jha, S</creator><general>Springer</general><general>Springer Nature B.V</general><general>Springer Verlag</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>20061001</creationdate><title>Spontaneous plant regeneration in transformed roots and calli from Tylophora indica: changes in morphological phenotype and tylophorine accumulation associated with transformation by Agrobacterium rhizogenes</title><author>Chaudhuri, K.N ; Ghosh, B ; Tepfer, D ; Jha, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-ce392fe8387784e6cb409bf3641bd0e96a072389126edbe4c4aa69acab1401733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Agrobacterium rhizogenes</topic><topic>Alkaloids - analysis</topic><topic>Alkaloids - biosynthesis</topic><topic>Alkaloids - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>callus culture</topic><topic>DNA, Bacterial - metabolism</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genetic transformation</topic><topic>Genetics</topic><topic>indolizidine alkaloids</topic><topic>Indolizines - analysis</topic><topic>Indolizines - metabolism</topic><topic>Life Sciences</topic><topic>Light</topic><topic>Medicinal plants</topic><topic>Methods. Procedures. Technologies</topic><topic>Miscellaneous</topic><topic>Phenanthrenes - analysis</topic><topic>Phenanthrenes - metabolism</topic><topic>Phenotype</topic><topic>phenotypic variation</topic><topic>Plant cells and fungal cells</topic><topic>plant growth</topic><topic>plant morphology</topic><topic>plant regeneration</topic><topic>Plant Roots - physiology</topic><topic>Plant Shoots - physiology</topic><topic>Plants genetics</topic><topic>Plants, Genetically Modified</topic><topic>Regeneration - radiation effects</topic><topic>Rhizobium - metabolism</topic><topic>Rhizobium rhizogenes</topic><topic>Roots</topic><topic>Shoots</topic><topic>Transformation, Genetic</topic><topic>Transgenic plants</topic><topic>Tylophora</topic><topic>Tylophora - genetics</topic><topic>Tylophora - growth & development</topic><topic>Tylophora - physiology</topic><topic>Tylophora - radiation effects</topic><topic>Tylophora indica</topic><topic>tylophorine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chaudhuri, K.N</creatorcontrib><creatorcontrib>Ghosh, B</creatorcontrib><creatorcontrib>Tepfer, D</creatorcontrib><creatorcontrib>Jha, S</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chaudhuri, K.N</au><au>Ghosh, B</au><au>Tepfer, D</au><au>Jha, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spontaneous plant regeneration in transformed roots and calli from Tylophora indica: changes in morphological phenotype and tylophorine accumulation associated with transformation by Agrobacterium rhizogenes</atitle><jtitle>Plant cell reports</jtitle><addtitle>Plant Cell Rep</addtitle><date>2006-10-01</date><risdate>2006</risdate><volume>25</volume><issue>10</issue><spage>1059</spage><epage>1066</epage><pages>1059-1066</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><coden>PCRPD8</coden><abstract>We examined the effects of genetic transformation by Agrobacterium rhizogenes on the production of tylophorine, a phenanthroindolizidine alkaloid, in the Indian medicinal plant, Tylophora indica. Transformed roots induced by the bacterium grew in axenic culture and produced shoots or embryogenic calli in the absence of hormone treatments. However, hormonal treatment was required to regenerate shoots in root explants of wild type control plants. Transformed plants showed morphological features typically seen in transgenic plants produced by A. rhizogenes, which include, short internodes, small and wrinkled leaves, more branches and numerous plagiotropic roots. Plants regenerated from transformed roots showed increased biomass accumulation (350-510% in the roots and 200-320% in the whole plants) and augmented tylophorine content (20-60%) in the shoots, resulting in a 160-280% increase in tylophorine production in different clones grown in vitro.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>16609889</pmid><doi>10.1007/s00299-006-0164-z</doi><tpages>8</tpages></addata></record> |
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| subjects | Agrobacterium rhizogenes Alkaloids - analysis Alkaloids - biosynthesis Alkaloids - metabolism Biological and medical sciences Biotechnology callus culture DNA, Bacterial - metabolism Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology genetic transformation Genetics indolizidine alkaloids Indolizines - analysis Indolizines - metabolism Life Sciences Light Medicinal plants Methods. Procedures. Technologies Miscellaneous Phenanthrenes - analysis Phenanthrenes - metabolism Phenotype phenotypic variation Plant cells and fungal cells plant growth plant morphology plant regeneration Plant Roots - physiology Plant Shoots - physiology Plants genetics Plants, Genetically Modified Regeneration - radiation effects Rhizobium - metabolism Rhizobium rhizogenes Roots Shoots Transformation, Genetic Transgenic plants Tylophora Tylophora - genetics Tylophora - growth & development Tylophora - physiology Tylophora - radiation effects Tylophora indica tylophorine |
| title | Spontaneous plant regeneration in transformed roots and calli from Tylophora indica: changes in morphological phenotype and tylophorine accumulation associated with transformation by Agrobacterium rhizogenes |
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