Advantages of Combining Low-Density Lipoproteins with Glutamine for Cryopreservation of Canine Semen
Twenty sperm samples from five dogs were frozen in liquid nitrogen at -196°C in 16 different media, two control media containing 20% egg yolk and 6% low-density lipoproteins (LDL); 10 test media containing 6% LDL (the active cryoprotective ingredient of chicken egg yolk) combined with 10, 20, 30, 40...
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| Veröffentlicht in: | Reproduction in domestic animals 2010-04, Vol.45 (2), p.189-200 |
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| creator | Bencharif, D Amirat, L Pascal, O Anton, M Schmitt, E Desherces, S Delhomme, G Langlois, M-L Barrière, P Larrat, M Tainturier, D |
| description | Twenty sperm samples from five dogs were frozen in liquid nitrogen at -196°C in 16 different media, two control media containing 20% egg yolk and 6% low-density lipoproteins (LDL); 10 test media containing 6% LDL (the active cryoprotective ingredient of chicken egg yolk) combined with 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 mmol of glutamine respectively at 4%, 5%, 7%, and 8% LDL. Following thawing, sperm mobility was assessed using an image analyser, HAMILTON THORN CERROS 12. The percentage of mobile spermatozoa was 62.05% in the 6% LDL + 20 mmol glutamine medium compared with 48.90% in the egg yolk-based medium (p < 0.05) or 57.55% for the 6% LDL medium (p < 0.05). Furthermore, in most cases, the motility parameters (average path velocity, curvilinear velocity, straight line velocity) in the 6% LDL + 20 mmol glutamine medium, were superior, to a statistically significant extent, to those in the control media. Finally, the 6% LDL + 20 mmol glutamine combination provides spermatozoa with better protection during freezing than egg yolk or the 6% LDL medium alone in terms of acrosome integrity (fluorescein isothiocyanate-Pisum sativum agglutinin test: p < 0.05), the flagellar plasma membrane (hypo-osmotic test: p < 0.05 for 6% LDL), the DNA (acridine orange test; no significant difference) and the integrity of the acrosome (Spermac® test: no significant difference). |
| doi_str_mv | 10.1111/j.1439-0531.2008.01198.x |
| format | Article |
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Following thawing, sperm mobility was assessed using an image analyser, HAMILTON THORN CERROS 12. The percentage of mobile spermatozoa was 62.05% in the 6% LDL + 20 mmol glutamine medium compared with 48.90% in the egg yolk-based medium (p < 0.05) or 57.55% for the 6% LDL medium (p < 0.05). Furthermore, in most cases, the motility parameters (average path velocity, curvilinear velocity, straight line velocity) in the 6% LDL + 20 mmol glutamine medium, were superior, to a statistically significant extent, to those in the control media. Finally, the 6% LDL + 20 mmol glutamine combination provides spermatozoa with better protection during freezing than egg yolk or the 6% LDL medium alone in terms of acrosome integrity (fluorescein isothiocyanate-Pisum sativum agglutinin test: p < 0.05), the flagellar plasma membrane (hypo-osmotic test: p < 0.05 for 6% LDL), the DNA (acridine orange test; no significant difference) and the integrity of the acrosome (Spermac® test: no significant difference).</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/j.1439-0531.2008.01198.x</identifier><identifier>PMID: 18992079</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Animal biology ; Animal reproduction ; Animals ; Biological and medical sciences ; Cryogenic engineering ; Cryopreservation - methods ; Cryopreservation - veterinary ; Cryoprotective Agents - pharmacology ; Dogs ; Dogs - physiology ; Dose-Response Relationship, Drug ; Fundamental and applied biological sciences. Psychology ; Glutamine - pharmacology ; Life Sciences ; Lipoproteins, LDL - pharmacology ; Low density lipoprotein ; Male ; Mammalian reproduction. General aspects ; Reproductive Biology ; Semen Preservation - methods ; Semen Preservation - veterinary ; Spermatozoa - cytology ; Spermatozoa - drug effects ; Spermatozoa - physiology ; Vertebrates: reproduction</subject><ispartof>Reproduction in domestic animals, 2010-04, Vol.45 (2), p.189-200</ispartof><rights>2008 The Authors. Journal compilation © 2008 Blackwell Verlag</rights><rights>2015 INIST-CNRS</rights><rights>2010 Blackwell Verlag GmbH</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5538-db6299ff36efce29da6dcad513206c1c8abfe01da178d262a950a92392ce612d3</citedby><cites>FETCH-LOGICAL-c5538-db6299ff36efce29da6dcad513206c1c8abfe01da178d262a950a92392ce612d3</cites><orcidid>0000-0002-6762-6679 ; 0000-0002-2825-2272 ; 0000-0003-0857-9769</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1439-0531.2008.01198.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1439-0531.2008.01198.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22491427$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18992079$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02663894$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Bencharif, D</creatorcontrib><creatorcontrib>Amirat, L</creatorcontrib><creatorcontrib>Pascal, O</creatorcontrib><creatorcontrib>Anton, M</creatorcontrib><creatorcontrib>Schmitt, E</creatorcontrib><creatorcontrib>Desherces, S</creatorcontrib><creatorcontrib>Delhomme, G</creatorcontrib><creatorcontrib>Langlois, M-L</creatorcontrib><creatorcontrib>Barrière, P</creatorcontrib><creatorcontrib>Larrat, M</creatorcontrib><creatorcontrib>Tainturier, D</creatorcontrib><title>Advantages of Combining Low-Density Lipoproteins with Glutamine for Cryopreservation of Canine Semen</title><title>Reproduction in domestic animals</title><addtitle>Reprod Domest Anim</addtitle><description>Twenty sperm samples from five dogs were frozen in liquid nitrogen at -196°C in 16 different media, two control media containing 20% egg yolk and 6% low-density lipoproteins (LDL); 10 test media containing 6% LDL (the active cryoprotective ingredient of chicken egg yolk) combined with 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 mmol of glutamine respectively at 4%, 5%, 7%, and 8% LDL. Following thawing, sperm mobility was assessed using an image analyser, HAMILTON THORN CERROS 12. The percentage of mobile spermatozoa was 62.05% in the 6% LDL + 20 mmol glutamine medium compared with 48.90% in the egg yolk-based medium (p < 0.05) or 57.55% for the 6% LDL medium (p < 0.05). Furthermore, in most cases, the motility parameters (average path velocity, curvilinear velocity, straight line velocity) in the 6% LDL + 20 mmol glutamine medium, were superior, to a statistically significant extent, to those in the control media. Finally, the 6% LDL + 20 mmol glutamine combination provides spermatozoa with better protection during freezing than egg yolk or the 6% LDL medium alone in terms of acrosome integrity (fluorescein isothiocyanate-Pisum sativum agglutinin test: p < 0.05), the flagellar plasma membrane (hypo-osmotic test: p < 0.05 for 6% LDL), the DNA (acridine orange test; no significant difference) and the integrity of the acrosome (Spermac® test: no significant difference).</description><subject>Animal biology</subject><subject>Animal reproduction</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cryogenic engineering</subject><subject>Cryopreservation - methods</subject><subject>Cryopreservation - veterinary</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>Dogs</subject><subject>Dogs - physiology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glutamine - pharmacology</subject><subject>Life Sciences</subject><subject>Lipoproteins, LDL - pharmacology</subject><subject>Low density lipoprotein</subject><subject>Male</subject><subject>Mammalian reproduction. General aspects</subject><subject>Reproductive Biology</subject><subject>Semen Preservation - methods</subject><subject>Semen Preservation - veterinary</subject><subject>Spermatozoa - cytology</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - physiology</subject><subject>Vertebrates: reproduction</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV1v0zAYhSMEYmXwFyBCQoiLFL924o-LXVTd1iEVkDYGl5abOJ1LYhc7_fr3OEtVJK7mG1t-n3N0Xp0kSQGNIZ7PqzHkRGSoIDDGCPExAhB8vH-WjE6D58kICUIzyig_S16FsEIICs7Yy-QMuBAYMTFKqkm1VbZTSx1SV6dT1y6MNXaZzt0uu9Q2mO6Qzs3arb3rtLEh3ZnuIZ01m061xuq0dj6d-kOc66D9VnXG2UcnZfvxnW61fZ28qFUT9JvjfZ7cX1_9mN5k8--zL9PJPCuLgvCsWlAsRF0TqutSY1EpWpWqKoBgREsouVrUGkGlgPEKU6xEgZTAROBSU8AVOU8-Db4PqpFrb1rlD9IpI28mc9n_IUwp4SLfQmQ_Dmxc7M9Gh062JpS6aZTVbhMky7ngOefiCWROGeSAI_n-P3LlNt7GlSUGwnKBOYsQH6DSuxC8rk9JAcm-XbmSfYmyL1H27crHduU-St8e_TeLVlf_hMc6I_DhCKhQqqb2ypYmnDiMcwE57jNcDNzONPrw5ADy9nLSv6I-G_QmdHp_0iv_W1JGWCF_fZvJr4Lhn1jM5G3k3w18rZxUSx8z3d9hBAQBB4pJTv4C3FPXWA</recordid><startdate>201004</startdate><enddate>201004</enddate><creator>Bencharif, D</creator><creator>Amirat, L</creator><creator>Pascal, O</creator><creator>Anton, M</creator><creator>Schmitt, E</creator><creator>Desherces, S</creator><creator>Delhomme, G</creator><creator>Langlois, M-L</creator><creator>Barrière, P</creator><creator>Larrat, M</creator><creator>Tainturier, D</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Wiley</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-6762-6679</orcidid><orcidid>https://orcid.org/0000-0002-2825-2272</orcidid><orcidid>https://orcid.org/0000-0003-0857-9769</orcidid></search><sort><creationdate>201004</creationdate><title>Advantages of Combining Low-Density Lipoproteins with Glutamine for Cryopreservation of Canine Semen</title><author>Bencharif, D ; Amirat, L ; Pascal, O ; Anton, M ; Schmitt, E ; Desherces, S ; Delhomme, G ; Langlois, M-L ; Barrière, P ; Larrat, M ; Tainturier, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5538-db6299ff36efce29da6dcad513206c1c8abfe01da178d262a950a92392ce612d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animal biology</topic><topic>Animal reproduction</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cryogenic engineering</topic><topic>Cryopreservation - methods</topic><topic>Cryopreservation - veterinary</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>Dogs</topic><topic>Dogs - physiology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glutamine - pharmacology</topic><topic>Life Sciences</topic><topic>Lipoproteins, LDL - pharmacology</topic><topic>Low density lipoprotein</topic><topic>Male</topic><topic>Mammalian reproduction. General aspects</topic><topic>Reproductive Biology</topic><topic>Semen Preservation - methods</topic><topic>Semen Preservation - veterinary</topic><topic>Spermatozoa - cytology</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - physiology</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bencharif, D</creatorcontrib><creatorcontrib>Amirat, L</creatorcontrib><creatorcontrib>Pascal, O</creatorcontrib><creatorcontrib>Anton, M</creatorcontrib><creatorcontrib>Schmitt, E</creatorcontrib><creatorcontrib>Desherces, S</creatorcontrib><creatorcontrib>Delhomme, G</creatorcontrib><creatorcontrib>Langlois, M-L</creatorcontrib><creatorcontrib>Barrière, P</creatorcontrib><creatorcontrib>Larrat, M</creatorcontrib><creatorcontrib>Tainturier, D</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bencharif, D</au><au>Amirat, L</au><au>Pascal, O</au><au>Anton, M</au><au>Schmitt, E</au><au>Desherces, S</au><au>Delhomme, G</au><au>Langlois, M-L</au><au>Barrière, P</au><au>Larrat, M</au><au>Tainturier, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Advantages of Combining Low-Density Lipoproteins with Glutamine for Cryopreservation of Canine Semen</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Domest Anim</addtitle><date>2010-04</date><risdate>2010</risdate><volume>45</volume><issue>2</issue><spage>189</spage><epage>200</epage><pages>189-200</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>Twenty sperm samples from five dogs were frozen in liquid nitrogen at -196°C in 16 different media, two control media containing 20% egg yolk and 6% low-density lipoproteins (LDL); 10 test media containing 6% LDL (the active cryoprotective ingredient of chicken egg yolk) combined with 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 mmol of glutamine respectively at 4%, 5%, 7%, and 8% LDL. Following thawing, sperm mobility was assessed using an image analyser, HAMILTON THORN CERROS 12. The percentage of mobile spermatozoa was 62.05% in the 6% LDL + 20 mmol glutamine medium compared with 48.90% in the egg yolk-based medium (p < 0.05) or 57.55% for the 6% LDL medium (p < 0.05). Furthermore, in most cases, the motility parameters (average path velocity, curvilinear velocity, straight line velocity) in the 6% LDL + 20 mmol glutamine medium, were superior, to a statistically significant extent, to those in the control media. Finally, the 6% LDL + 20 mmol glutamine combination provides spermatozoa with better protection during freezing than egg yolk or the 6% LDL medium alone in terms of acrosome integrity (fluorescein isothiocyanate-Pisum sativum agglutinin test: p < 0.05), the flagellar plasma membrane (hypo-osmotic test: p < 0.05 for 6% LDL), the DNA (acridine orange test; no significant difference) and the integrity of the acrosome (Spermac® test: no significant difference).</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>18992079</pmid><doi>10.1111/j.1439-0531.2008.01198.x</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-6762-6679</orcidid><orcidid>https://orcid.org/0000-0002-2825-2272</orcidid><orcidid>https://orcid.org/0000-0003-0857-9769</orcidid></addata></record> |
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| subjects | Animal biology Animal reproduction Animals Biological and medical sciences Cryogenic engineering Cryopreservation - methods Cryopreservation - veterinary Cryoprotective Agents - pharmacology Dogs Dogs - physiology Dose-Response Relationship, Drug Fundamental and applied biological sciences. Psychology Glutamine - pharmacology Life Sciences Lipoproteins, LDL - pharmacology Low density lipoprotein Male Mammalian reproduction. General aspects Reproductive Biology Semen Preservation - methods Semen Preservation - veterinary Spermatozoa - cytology Spermatozoa - drug effects Spermatozoa - physiology Vertebrates: reproduction |
| title | Advantages of Combining Low-Density Lipoproteins with Glutamine for Cryopreservation of Canine Semen |
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