Dynamics of acetylcholinesterase activity recovery in two earthworm species following exposure to ethyl-parathion
In order for cholinesterase (ChE) activity to be used as an effective biomarker in earthworms, the time course of enzyme activity inhibition and recovery must be fully characterized. A laboratory experiment was carried out using parathion as a model organophosphorus pesticide at the recommended dose...
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| Veröffentlicht in: | Soil biology & biochemistry 2008-12, Vol.40 (12), p.3086-3091 |
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| description | In order for cholinesterase (ChE) activity to be used as an effective biomarker in earthworms, the time course of enzyme activity inhibition and recovery must be fully characterized. A laboratory experiment was carried out using parathion as a model organophosphorus pesticide at the recommended dose (1
mg
kg
−1) and a 10 fold higher dose (10
mg
kg
−1), on two earthworm species (
Allolobophora chlorotica and
Aporrectodea caliginosa). ChE activity and weight were measured every week for a 14
day period of exposure to parathion and then for 8
weeks in uncontaminated soil. After 3
days of exposure, the weight of both earthworm species had decreased by 10–15% compared to the control, regardless of the dose used. During the remainder of the exposure period, no differences were observed between the two doses for
A. chlorotica; but
A. caliginosa showed rapid weight recuperation for the lowest dose applied. After 28
days and over, the control and both exposed species of worms lost similar amounts of weight. ChE inhibition was measured during and after the exposure period. ChE inhibition followed a different time course for the two species investigated.
A. chlorotica appeared less sensitive to parathion than
A. caliginosa. In this latter species, ChE inhibition was rapid at close to 70% of the control after 3
days, for either dose, and reached 80–90% after 7
days exposure. While
A. chlorotica exhibited the same pattern of inhibition for 10
mg
kg
−1 of parathion, the inhibition process was slower for the recommended dose with 50% inhibition after 7
days of exposure and 70% after 14
days. ChE activity recovery, after transfer to uncontaminated soil, also followed a different pattern for the two species. After exposure to 1
mg
kg
−1 parathion, ChE activity from
A. chlorotica underwent a slow but constant recovery process to regain the control value after 8
weeks in unpolluted conditions. On the other hand, the ChE activity from
A. caliginosa remained strongly inhibited. The differential susceptibility to parathion found in this study could be related to differences in the specificity of the total ChE activities between those two species. |
| doi_str_mv | 10.1016/j.soilbio.2008.09.010 |
| format | Article |
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mg
kg
−1) and a 10 fold higher dose (10
mg
kg
−1), on two earthworm species (
Allolobophora chlorotica and
Aporrectodea caliginosa). ChE activity and weight were measured every week for a 14
day period of exposure to parathion and then for 8
weeks in uncontaminated soil. After 3
days of exposure, the weight of both earthworm species had decreased by 10–15% compared to the control, regardless of the dose used. During the remainder of the exposure period, no differences were observed between the two doses for
A. chlorotica; but
A. caliginosa showed rapid weight recuperation for the lowest dose applied. After 28
days and over, the control and both exposed species of worms lost similar amounts of weight. ChE inhibition was measured during and after the exposure period. ChE inhibition followed a different time course for the two species investigated.
A. chlorotica appeared less sensitive to parathion than
A. caliginosa. In this latter species, ChE inhibition was rapid at close to 70% of the control after 3
days, for either dose, and reached 80–90% after 7
days exposure. While
A. chlorotica exhibited the same pattern of inhibition for 10
mg
kg
−1 of parathion, the inhibition process was slower for the recommended dose with 50% inhibition after 7
days of exposure and 70% after 14
days. ChE activity recovery, after transfer to uncontaminated soil, also followed a different pattern for the two species. After exposure to 1
mg
kg
−1 parathion, ChE activity from
A. chlorotica underwent a slow but constant recovery process to regain the control value after 8
weeks in unpolluted conditions. On the other hand, the ChE activity from
A. caliginosa remained strongly inhibited. The differential susceptibility to parathion found in this study could be related to differences in the specificity of the total ChE activities between those two species.</description><identifier>ISSN: 0038-0717</identifier><identifier>EISSN: 1879-3428</identifier><identifier>DOI: 10.1016/j.soilbio.2008.09.010</identifier><identifier>CODEN: SBIOAH</identifier><language>eng</language><publisher>Amsterdam: Elsevier Ltd</publisher><subject>Acetylcholinesterase ; Agricultural sciences ; Agronomy. Soil science and plant productions ; Allolobophora chlorotica ; Aporrectodea caliginosa ; Biochemistry and biology ; Biological and medical sciences ; Biomarker ; biomarkers ; body weight ; Chemical, physicochemical, biochemical and biological properties ; dose response ; Earthworms ; environmental exposure ; enzyme activity ; enzyme inhibition ; Fundamental and applied biological sciences. Psychology ; in vivo studies ; laboratories ; Life Sciences ; nontarget organisms ; Organophosphate ; parathion ; Physics, chemistry, biochemistry and biology of agricultural and forest soils ; polluted soils ; Soil science ; Soil study ; toxicity testing ; Zoology (interactions between soil fauna and agricultural or forest soils)</subject><ispartof>Soil biology & biochemistry, 2008-12, Vol.40 (12), p.3086-3091</ispartof><rights>2008 Elsevier Ltd</rights><rights>2009 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c494t-99a695211e77469ade41a6eea3596a91c2f0e8920aa31ad2469bc25640cfa6a53</citedby><orcidid>0000-0001-5580-0687 ; 0000-0001-5495-5775 ; 0000-0003-4454-3034</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.soilbio.2008.09.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,777,781,882,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20912343$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02663564$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Rault, Magali</creatorcontrib><creatorcontrib>Collange, Béatrice</creatorcontrib><creatorcontrib>Mazzia, Christophe</creatorcontrib><creatorcontrib>Capowiez, Yvan</creatorcontrib><title>Dynamics of acetylcholinesterase activity recovery in two earthworm species following exposure to ethyl-parathion</title><title>Soil biology & biochemistry</title><description>In order for cholinesterase (ChE) activity to be used as an effective biomarker in earthworms, the time course of enzyme activity inhibition and recovery must be fully characterized. A laboratory experiment was carried out using parathion as a model organophosphorus pesticide at the recommended dose (1
mg
kg
−1) and a 10 fold higher dose (10
mg
kg
−1), on two earthworm species (
Allolobophora chlorotica and
Aporrectodea caliginosa). ChE activity and weight were measured every week for a 14
day period of exposure to parathion and then for 8
weeks in uncontaminated soil. After 3
days of exposure, the weight of both earthworm species had decreased by 10–15% compared to the control, regardless of the dose used. During the remainder of the exposure period, no differences were observed between the two doses for
A. chlorotica; but
A. caliginosa showed rapid weight recuperation for the lowest dose applied. After 28
days and over, the control and both exposed species of worms lost similar amounts of weight. ChE inhibition was measured during and after the exposure period. ChE inhibition followed a different time course for the two species investigated.
A. chlorotica appeared less sensitive to parathion than
A. caliginosa. In this latter species, ChE inhibition was rapid at close to 70% of the control after 3
days, for either dose, and reached 80–90% after 7
days exposure. While
A. chlorotica exhibited the same pattern of inhibition for 10
mg
kg
−1 of parathion, the inhibition process was slower for the recommended dose with 50% inhibition after 7
days of exposure and 70% after 14
days. ChE activity recovery, after transfer to uncontaminated soil, also followed a different pattern for the two species. After exposure to 1
mg
kg
−1 parathion, ChE activity from
A. chlorotica underwent a slow but constant recovery process to regain the control value after 8
weeks in unpolluted conditions. On the other hand, the ChE activity from
A. caliginosa remained strongly inhibited. The differential susceptibility to parathion found in this study could be related to differences in the specificity of the total ChE activities between those two species.</description><subject>Acetylcholinesterase</subject><subject>Agricultural sciences</subject><subject>Agronomy. Soil science and plant productions</subject><subject>Allolobophora chlorotica</subject><subject>Aporrectodea caliginosa</subject><subject>Biochemistry and biology</subject><subject>Biological and medical sciences</subject><subject>Biomarker</subject><subject>biomarkers</subject><subject>body weight</subject><subject>Chemical, physicochemical, biochemical and biological properties</subject><subject>dose response</subject><subject>Earthworms</subject><subject>environmental exposure</subject><subject>enzyme activity</subject><subject>enzyme inhibition</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>in vivo studies</subject><subject>laboratories</subject><subject>Life Sciences</subject><subject>nontarget organisms</subject><subject>Organophosphate</subject><subject>parathion</subject><subject>Physics, chemistry, biochemistry and biology of agricultural and forest soils</subject><subject>polluted soils</subject><subject>Soil science</subject><subject>Soil study</subject><subject>toxicity testing</subject><subject>Zoology (interactions between soil fauna and agricultural or forest soils)</subject><issn>0038-0717</issn><issn>1879-3428</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNqFkcGO0zAQhiMEEmXhERC-gMQhZewkTnxCq11gkSpxgD1bs-5k48qNs7bbkrfHVaq9crI0-v4Zz3xF8Z7DmgOXX3br6K17sH4tALo1qDVweFGseNeqsqpF97JYAVRdCS1vXxdvYtwBgGh4tSqebucR99ZE5nuGhtLszOCdHSkmChgpF5M92jSzQMYfKczMjiydPCMMaTj5sGdxImMpst475092fGT0d_LxEIilzKVhduWEAdNg_fi2eNWji_Tu8l4V99-__bm5Kze_fvy8ud6UplZ1KpVCqRrBObVtLRVuqeYoibBqlETFjeiBOiUAseK4FZl5MKKRNZgeJTbVVfF56Tug01Owewyz9mj13fVGn2sgpKxy4Mgz-2lhp-CfDnl1vbfRkHM4kj9EzZUUXSchg80CmuBjDNQ_d-agzzL0Tl9k6LMMDUpnGTn38TIAo0HXBxyNjc9hAYqLqq4y92HhevQaH0Nm7n8L4BXwRoIU57W-LgTl2x0tBR3z7UdDW5sFJb319j9_-Qd2Hq3s</recordid><startdate>20081201</startdate><enddate>20081201</enddate><creator>Rault, Magali</creator><creator>Collange, Béatrice</creator><creator>Mazzia, Christophe</creator><creator>Capowiez, Yvan</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SN</scope><scope>7TV</scope><scope>C1K</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-5580-0687</orcidid><orcidid>https://orcid.org/0000-0001-5495-5775</orcidid><orcidid>https://orcid.org/0000-0003-4454-3034</orcidid></search><sort><creationdate>20081201</creationdate><title>Dynamics of acetylcholinesterase activity recovery in two earthworm species following exposure to ethyl-parathion</title><author>Rault, Magali ; Collange, Béatrice ; Mazzia, Christophe ; Capowiez, Yvan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c494t-99a695211e77469ade41a6eea3596a91c2f0e8920aa31ad2469bc25640cfa6a53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Acetylcholinesterase</topic><topic>Agricultural sciences</topic><topic>Agronomy. Soil science and plant productions</topic><topic>Allolobophora chlorotica</topic><topic>Aporrectodea caliginosa</topic><topic>Biochemistry and biology</topic><topic>Biological and medical sciences</topic><topic>Biomarker</topic><topic>biomarkers</topic><topic>body weight</topic><topic>Chemical, physicochemical, biochemical and biological properties</topic><topic>dose response</topic><topic>Earthworms</topic><topic>environmental exposure</topic><topic>enzyme activity</topic><topic>enzyme inhibition</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>in vivo studies</topic><topic>laboratories</topic><topic>Life Sciences</topic><topic>nontarget organisms</topic><topic>Organophosphate</topic><topic>parathion</topic><topic>Physics, chemistry, biochemistry and biology of agricultural and forest soils</topic><topic>polluted soils</topic><topic>Soil science</topic><topic>Soil study</topic><topic>toxicity testing</topic><topic>Zoology (interactions between soil fauna and agricultural or forest soils)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rault, Magali</creatorcontrib><creatorcontrib>Collange, Béatrice</creatorcontrib><creatorcontrib>Mazzia, Christophe</creatorcontrib><creatorcontrib>Capowiez, Yvan</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Ecology Abstracts</collection><collection>Pollution Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Soil biology & biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rault, Magali</au><au>Collange, Béatrice</au><au>Mazzia, Christophe</au><au>Capowiez, Yvan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dynamics of acetylcholinesterase activity recovery in two earthworm species following exposure to ethyl-parathion</atitle><jtitle>Soil biology & biochemistry</jtitle><date>2008-12-01</date><risdate>2008</risdate><volume>40</volume><issue>12</issue><spage>3086</spage><epage>3091</epage><pages>3086-3091</pages><issn>0038-0717</issn><eissn>1879-3428</eissn><coden>SBIOAH</coden><abstract>In order for cholinesterase (ChE) activity to be used as an effective biomarker in earthworms, the time course of enzyme activity inhibition and recovery must be fully characterized. A laboratory experiment was carried out using parathion as a model organophosphorus pesticide at the recommended dose (1
mg
kg
−1) and a 10 fold higher dose (10
mg
kg
−1), on two earthworm species (
Allolobophora chlorotica and
Aporrectodea caliginosa). ChE activity and weight were measured every week for a 14
day period of exposure to parathion and then for 8
weeks in uncontaminated soil. After 3
days of exposure, the weight of both earthworm species had decreased by 10–15% compared to the control, regardless of the dose used. During the remainder of the exposure period, no differences were observed between the two doses for
A. chlorotica; but
A. caliginosa showed rapid weight recuperation for the lowest dose applied. After 28
days and over, the control and both exposed species of worms lost similar amounts of weight. ChE inhibition was measured during and after the exposure period. ChE inhibition followed a different time course for the two species investigated.
A. chlorotica appeared less sensitive to parathion than
A. caliginosa. In this latter species, ChE inhibition was rapid at close to 70% of the control after 3
days, for either dose, and reached 80–90% after 7
days exposure. While
A. chlorotica exhibited the same pattern of inhibition for 10
mg
kg
−1 of parathion, the inhibition process was slower for the recommended dose with 50% inhibition after 7
days of exposure and 70% after 14
days. ChE activity recovery, after transfer to uncontaminated soil, also followed a different pattern for the two species. After exposure to 1
mg
kg
−1 parathion, ChE activity from
A. chlorotica underwent a slow but constant recovery process to regain the control value after 8
weeks in unpolluted conditions. On the other hand, the ChE activity from
A. caliginosa remained strongly inhibited. The differential susceptibility to parathion found in this study could be related to differences in the specificity of the total ChE activities between those two species.</abstract><cop>Amsterdam</cop><pub>Elsevier Ltd</pub><doi>10.1016/j.soilbio.2008.09.010</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0001-5580-0687</orcidid><orcidid>https://orcid.org/0000-0001-5495-5775</orcidid><orcidid>https://orcid.org/0000-0003-4454-3034</orcidid></addata></record> |
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| ispartof | Soil biology & biochemistry, 2008-12, Vol.40 (12), p.3086-3091 |
| issn | 0038-0717 1879-3428 |
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| source | Elsevier ScienceDirect Journals |
| subjects | Acetylcholinesterase Agricultural sciences Agronomy. Soil science and plant productions Allolobophora chlorotica Aporrectodea caliginosa Biochemistry and biology Biological and medical sciences Biomarker biomarkers body weight Chemical, physicochemical, biochemical and biological properties dose response Earthworms environmental exposure enzyme activity enzyme inhibition Fundamental and applied biological sciences. Psychology in vivo studies laboratories Life Sciences nontarget organisms Organophosphate parathion Physics, chemistry, biochemistry and biology of agricultural and forest soils polluted soils Soil science Soil study toxicity testing Zoology (interactions between soil fauna and agricultural or forest soils) |
| title | Dynamics of acetylcholinesterase activity recovery in two earthworm species following exposure to ethyl-parathion |
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