Exploring the acidotolerance of β-galactosidase from Lactobacillus delbrueckii subsp. bulgaricus: an attractive enzyme for lactose bioconversion

The LacZ gene encoding β-galactosidase from Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 ( L. bulgaricus) was cloned, sequenced and expressed in Escherichia coli, followed by purification and characterization of the protein. The recombinant enzyme was shown to be a homotetramer and could b...

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Veröffentlicht in:	Research in microbiology 2009-12, Vol.160 (10), p.775-784
Hauptverfasser:	Rhimi, Moez, Aghajari, Nushin, Jaouadi, Bassem, Juy, Michel, Boudebbouze, Samira, Maguin, Emmanuelle, Haser, Richard, Bejar, Samir
Format:	Artikel
Sprache:	eng
Schlagworte:	Acidotolerance beta-Galactosidase - genetics beta-Galactosidase - isolation & purification beta-Galactosidase - metabolism Biological and medical sciences Biotransformation Cloning, Molecular Fundamental and applied biological sciences. Psychology Gene Expression Genes, Bacterial Hydrogen-Ion Concentration Hydrolysis - drug effects Lac Operon Lactobacillus delbrueckii - enzymology Lactobacillus delbrueckii - genetics Lactose - metabolism Lactose hydrolysis Life Sciences Microbiology Molecular modeling Sequence Analysis, DNA Site-directed mutagenesis Temperature β-Galactosidase
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