improved protocol for electroporation of Oenococcus oeni ATCC BAA-1163 using ethanol as immediate membrane fluidizing agent
To finalize an effective and reproducible electroporation procedure to transform Oenococcus oeni ATCC BAA-1163 strain. The vector pGID052 was selected to optimize the electroporation procedure. Transformation efficiency was 5·8 x 10³ per μg of DNA. Transformation was improved when competent cells we...
Gespeichert in:
| Veröffentlicht in: | Letters in applied microbiology 2008-10, Vol.47 (4), p.333-338 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 338 |
|---|---|
| container_issue | 4 |
| container_start_page | 333 |
| container_title | Letters in applied microbiology |
| container_volume | 47 |
| creator | Assad-García, J.S Bonnin-Jusserand, M Garmyn, D Guzzo, J Alexandre, H Grandvalet, C |
| description | To finalize an effective and reproducible electroporation procedure to transform Oenococcus oeni ATCC BAA-1163 strain. The vector pGID052 was selected to optimize the electroporation procedure. Transformation efficiency was 5·8 x 10³ per μg of DNA. Transformation was improved when competent cells were prepared with exponential phase cultures; optimum electroporation parameters were an electric pulse of 12·5 kV cm⁻¹, under a resistance of 200 Ω and the presence of 10% (v/v) ethanol in the electroporation buffer (EPB). An effective protocol to transform O. oeni ATCC BAA-1163 strain by electroporation has been obtained by addition of ethanol to the EPB. A heterologous expression was obtained in O. oeni ATCC BAA-1163 by introducing a recombinant vector encoding a truncated form of ClpL2 protein. This is the first report of a successful electroporation of O. oeni ATCC BAA-1163. The major improvement was the addition of ethanol to the EPB, which has never been reported before as means of enhancing the incorporation of foreign DNA molecules into prokaryote cells by electroporation. This method constitutes a useful tool for the genetic study of this lactic bacterium. |
| doi_str_mv | 10.1111/j.1472-765X.2008.02435.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_02663266v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>66696705</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4865-1c5e8d03a5f98eb1b78378843abfcd45d3cc9103bd67b2009b169e0e50f469583</originalsourceid><addsrcrecordid>eNqNkU2P1CAYx4nRuOPqV1AumnjoyEuhcPDQnahrMmYP7ibeCKUwy6QtI7Trrn55qZ2MRyUhEJ7f88L_DwDEaI3zerdf47IiRcXZtzVBSKwRKSlb3z8Cq1PgMVghwnkhCCvPwLOU9iiTmMin4AxLUmJG5Ar88v0hhjvbwnyMwYQOuhCh7awZYziEqEcfBhgcvLJDDhszJRjs4GF9vdnAi7ouMOYUTskPO2jHWz3kEjpB3_e29Xq0sLd9E_Vgoesm3_qfM6h3dhifgydOd8m-OJ7n4Objh-vNZbG9-vR5U28LUwrOCmyYFS2imjkpbIObStBKiJLqxpm2ZC01RmJEm5ZXTVZDNphLiyxDruSSCXoO3i51b3WnDtH3Oj6ooL26rLdqfpt1onnf4cy-Wdgsx_fJplH1PhnbdfkDYUqKcy55hdg_QSwpkZiSDIoFNDGkFK07jYCRmt1UezWbpmbT1Oym-uOmus-pL489piar-TfxaF8GXh8BnYzuXJbZ-HTiCOKiyv5n7v3C_fCdffjvAdS2_jLfcv6rJd_poPQu5h43XwnCFGFGs8SS_gbxqMOD</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19329132</pqid></control><display><type>article</type><title>improved protocol for electroporation of Oenococcus oeni ATCC BAA-1163 using ethanol as immediate membrane fluidizing agent</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Assad-García, J.S ; Bonnin-Jusserand, M ; Garmyn, D ; Guzzo, J ; Alexandre, H ; Grandvalet, C</creator><creatorcontrib>Assad-García, J.S ; Bonnin-Jusserand, M ; Garmyn, D ; Guzzo, J ; Alexandre, H ; Grandvalet, C</creatorcontrib><description>To finalize an effective and reproducible electroporation procedure to transform Oenococcus oeni ATCC BAA-1163 strain. The vector pGID052 was selected to optimize the electroporation procedure. Transformation efficiency was 5·8 x 10³ per μg of DNA. Transformation was improved when competent cells were prepared with exponential phase cultures; optimum electroporation parameters were an electric pulse of 12·5 kV cm⁻¹, under a resistance of 200 Ω and the presence of 10% (v/v) ethanol in the electroporation buffer (EPB). An effective protocol to transform O. oeni ATCC BAA-1163 strain by electroporation has been obtained by addition of ethanol to the EPB. A heterologous expression was obtained in O. oeni ATCC BAA-1163 by introducing a recombinant vector encoding a truncated form of ClpL2 protein. This is the first report of a successful electroporation of O. oeni ATCC BAA-1163. The major improvement was the addition of ethanol to the EPB, which has never been reported before as means of enhancing the incorporation of foreign DNA molecules into prokaryote cells by electroporation. This method constitutes a useful tool for the genetic study of this lactic bacterium.</description><identifier>ISSN: 0266-8254</identifier><identifier>EISSN: 1472-765X</identifier><identifier>DOI: 10.1111/j.1472-765X.2008.02435.x</identifier><identifier>PMID: 19241529</identifier><identifier>CODEN: LAMIE7</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Bacterial Proteins - genetics ; Biological and medical sciences ; Cell Membrane - drug effects ; Cell Membrane Permeability - drug effects ; DNA, Bacterial - analysis ; DNA, Bacterial - genetics ; electroporation ; Electroporation - methods ; Ethanol - pharmacology ; Fundamental and applied biological sciences. Psychology ; Gram-Positive Asporogenous Rods - drug effects ; Gram-Positive Asporogenous Rods - genetics ; Life Sciences ; membrane fluidizing agent ; Microbiology ; Microbiology and Parasitology ; Oenococcus oeni ; Plasmids - genetics</subject><ispartof>Letters in applied microbiology, 2008-10, Vol.47 (4), p.333-338</ispartof><rights>2008 The Authors. Journal compilation © 2008 The Society for Applied Microbiology</rights><rights>2008 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4865-1c5e8d03a5f98eb1b78378843abfcd45d3cc9103bd67b2009b169e0e50f469583</citedby><cites>FETCH-LOGICAL-c4865-1c5e8d03a5f98eb1b78378843abfcd45d3cc9103bd67b2009b169e0e50f469583</cites><orcidid>0000-0002-1687-455X ; 0000-0002-9974-7838</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1472-765X.2008.02435.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1472-765X.2008.02435.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20687825$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19241529$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02663266$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Assad-García, J.S</creatorcontrib><creatorcontrib>Bonnin-Jusserand, M</creatorcontrib><creatorcontrib>Garmyn, D</creatorcontrib><creatorcontrib>Guzzo, J</creatorcontrib><creatorcontrib>Alexandre, H</creatorcontrib><creatorcontrib>Grandvalet, C</creatorcontrib><title>improved protocol for electroporation of Oenococcus oeni ATCC BAA-1163 using ethanol as immediate membrane fluidizing agent</title><title>Letters in applied microbiology</title><addtitle>Lett Appl Microbiol</addtitle><description>To finalize an effective and reproducible electroporation procedure to transform Oenococcus oeni ATCC BAA-1163 strain. The vector pGID052 was selected to optimize the electroporation procedure. Transformation efficiency was 5·8 x 10³ per μg of DNA. Transformation was improved when competent cells were prepared with exponential phase cultures; optimum electroporation parameters were an electric pulse of 12·5 kV cm⁻¹, under a resistance of 200 Ω and the presence of 10% (v/v) ethanol in the electroporation buffer (EPB). An effective protocol to transform O. oeni ATCC BAA-1163 strain by electroporation has been obtained by addition of ethanol to the EPB. A heterologous expression was obtained in O. oeni ATCC BAA-1163 by introducing a recombinant vector encoding a truncated form of ClpL2 protein. This is the first report of a successful electroporation of O. oeni ATCC BAA-1163. The major improvement was the addition of ethanol to the EPB, which has never been reported before as means of enhancing the incorporation of foreign DNA molecules into prokaryote cells by electroporation. This method constitutes a useful tool for the genetic study of this lactic bacterium.</description><subject>Bacterial Proteins - genetics</subject><subject>Biological and medical sciences</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane Permeability - drug effects</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Bacterial - genetics</subject><subject>electroporation</subject><subject>Electroporation - methods</subject><subject>Ethanol - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gram-Positive Asporogenous Rods - drug effects</subject><subject>Gram-Positive Asporogenous Rods - genetics</subject><subject>Life Sciences</subject><subject>membrane fluidizing agent</subject><subject>Microbiology</subject><subject>Microbiology and Parasitology</subject><subject>Oenococcus oeni</subject><subject>Plasmids - genetics</subject><issn>0266-8254</issn><issn>1472-765X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU2P1CAYx4nRuOPqV1AumnjoyEuhcPDQnahrMmYP7ibeCKUwy6QtI7Trrn55qZ2MRyUhEJ7f88L_DwDEaI3zerdf47IiRcXZtzVBSKwRKSlb3z8Cq1PgMVghwnkhCCvPwLOU9iiTmMin4AxLUmJG5Ar88v0hhjvbwnyMwYQOuhCh7awZYziEqEcfBhgcvLJDDhszJRjs4GF9vdnAi7ouMOYUTskPO2jHWz3kEjpB3_e29Xq0sLd9E_Vgoesm3_qfM6h3dhifgydOd8m-OJ7n4Objh-vNZbG9-vR5U28LUwrOCmyYFS2imjkpbIObStBKiJLqxpm2ZC01RmJEm5ZXTVZDNphLiyxDruSSCXoO3i51b3WnDtH3Oj6ooL26rLdqfpt1onnf4cy-Wdgsx_fJplH1PhnbdfkDYUqKcy55hdg_QSwpkZiSDIoFNDGkFK07jYCRmt1UezWbpmbT1Oym-uOmus-pL489piar-TfxaF8GXh8BnYzuXJbZ-HTiCOKiyv5n7v3C_fCdffjvAdS2_jLfcv6rJd_poPQu5h43XwnCFGFGs8SS_gbxqMOD</recordid><startdate>200810</startdate><enddate>200810</enddate><creator>Assad-García, J.S</creator><creator>Bonnin-Jusserand, M</creator><creator>Garmyn, D</creator><creator>Guzzo, J</creator><creator>Alexandre, H</creator><creator>Grandvalet, C</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-1687-455X</orcidid><orcidid>https://orcid.org/0000-0002-9974-7838</orcidid></search><sort><creationdate>200810</creationdate><title>improved protocol for electroporation of Oenococcus oeni ATCC BAA-1163 using ethanol as immediate membrane fluidizing agent</title><author>Assad-García, J.S ; Bonnin-Jusserand, M ; Garmyn, D ; Guzzo, J ; Alexandre, H ; Grandvalet, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4865-1c5e8d03a5f98eb1b78378843abfcd45d3cc9103bd67b2009b169e0e50f469583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Biological and medical sciences</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane Permeability - drug effects</topic><topic>DNA, Bacterial - analysis</topic><topic>DNA, Bacterial - genetics</topic><topic>electroporation</topic><topic>Electroporation - methods</topic><topic>Ethanol - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gram-Positive Asporogenous Rods - drug effects</topic><topic>Gram-Positive Asporogenous Rods - genetics</topic><topic>Life Sciences</topic><topic>membrane fluidizing agent</topic><topic>Microbiology</topic><topic>Microbiology and Parasitology</topic><topic>Oenococcus oeni</topic><topic>Plasmids - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Assad-García, J.S</creatorcontrib><creatorcontrib>Bonnin-Jusserand, M</creatorcontrib><creatorcontrib>Garmyn, D</creatorcontrib><creatorcontrib>Guzzo, J</creatorcontrib><creatorcontrib>Alexandre, H</creatorcontrib><creatorcontrib>Grandvalet, C</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Letters in applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Assad-García, J.S</au><au>Bonnin-Jusserand, M</au><au>Garmyn, D</au><au>Guzzo, J</au><au>Alexandre, H</au><au>Grandvalet, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>improved protocol for electroporation of Oenococcus oeni ATCC BAA-1163 using ethanol as immediate membrane fluidizing agent</atitle><jtitle>Letters in applied microbiology</jtitle><addtitle>Lett Appl Microbiol</addtitle><date>2008-10</date><risdate>2008</risdate><volume>47</volume><issue>4</issue><spage>333</spage><epage>338</epage><pages>333-338</pages><issn>0266-8254</issn><eissn>1472-765X</eissn><coden>LAMIE7</coden><abstract>To finalize an effective and reproducible electroporation procedure to transform Oenococcus oeni ATCC BAA-1163 strain. The vector pGID052 was selected to optimize the electroporation procedure. Transformation efficiency was 5·8 x 10³ per μg of DNA. Transformation was improved when competent cells were prepared with exponential phase cultures; optimum electroporation parameters were an electric pulse of 12·5 kV cm⁻¹, under a resistance of 200 Ω and the presence of 10% (v/v) ethanol in the electroporation buffer (EPB). An effective protocol to transform O. oeni ATCC BAA-1163 strain by electroporation has been obtained by addition of ethanol to the EPB. A heterologous expression was obtained in O. oeni ATCC BAA-1163 by introducing a recombinant vector encoding a truncated form of ClpL2 protein. This is the first report of a successful electroporation of O. oeni ATCC BAA-1163. The major improvement was the addition of ethanol to the EPB, which has never been reported before as means of enhancing the incorporation of foreign DNA molecules into prokaryote cells by electroporation. This method constitutes a useful tool for the genetic study of this lactic bacterium.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>19241529</pmid><doi>10.1111/j.1472-765X.2008.02435.x</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-1687-455X</orcidid><orcidid>https://orcid.org/0000-0002-9974-7838</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0266-8254 |
| ispartof | Letters in applied microbiology, 2008-10, Vol.47 (4), p.333-338 |
| issn | 0266-8254 1472-765X |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_02663266v1 |
| source | MEDLINE; Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Bacterial Proteins - genetics Biological and medical sciences Cell Membrane - drug effects Cell Membrane Permeability - drug effects DNA, Bacterial - analysis DNA, Bacterial - genetics electroporation Electroporation - methods Ethanol - pharmacology Fundamental and applied biological sciences. Psychology Gram-Positive Asporogenous Rods - drug effects Gram-Positive Asporogenous Rods - genetics Life Sciences membrane fluidizing agent Microbiology Microbiology and Parasitology Oenococcus oeni Plasmids - genetics |
| title | improved protocol for electroporation of Oenococcus oeni ATCC BAA-1163 using ethanol as immediate membrane fluidizing agent |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-31T00%3A26%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=improved%20protocol%20for%20electroporation%20of%20Oenococcus%20oeni%20ATCC%20BAA-1163%20using%20ethanol%20as%20immediate%20membrane%20fluidizing%20agent&rft.jtitle=Letters%20in%20applied%20microbiology&rft.au=Assad-Garc%C3%ADa,%20J.S&rft.date=2008-10&rft.volume=47&rft.issue=4&rft.spage=333&rft.epage=338&rft.pages=333-338&rft.issn=0266-8254&rft.eissn=1472-765X&rft.coden=LAMIE7&rft_id=info:doi/10.1111/j.1472-765X.2008.02435.x&rft_dat=%3Cproquest_hal_p%3E66696705%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19329132&rft_id=info:pmid/19241529&rfr_iscdi=true |