17 beta-estradiol activates rapid signaling pathways involved in rat pachytene spermatocytes apoptosis through GPR30 and ER alpha
Aim of the present study was to investigate whether estrogens were able to directly activate rapid signaling pathways controlling spermatogenesis in rat pachytene spermatocytes (PS). Classically, estrogens act by binding to estrogen receptors (ERs) alpha and beta. Recently, it has been demonstrated...
Gespeichert in:
| Veröffentlicht in: | Molecular and cellular endocrinology 2010-05, Vol.320 (1-2), p.136-144 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 144 |
|---|---|
| container_issue | 1-2 |
| container_start_page | 136 |
| container_title | Molecular and cellular endocrinology |
| container_volume | 320 |
| creator | Chimento, Adele Sirianni, Rosa Delalande, Christelle Silandre, Dorothèe Bois, Camille Andò, Sebastiano Maggiolini, Marcello Carreau, Serge Pezzi, Vincenzo |
| description | Aim of the present study was to investigate whether estrogens were able to directly activate rapid signaling pathways controlling spermatogenesis in rat pachytene spermatocytes (PS). Classically, estrogens act by binding to estrogen receptors (ERs) alpha and beta. Recently, it has been demonstrated that rapid estrogen action can also be activated through the G-protein-coupled receptor (GPR)-30. Herein, we demonstrated that rat PS express ER alpha, ER beta and GPR30. Treatment of PS with estradiol (E2), the selective GPR30 agonist G1 and the selective ER alpha agonist PPT determined activation of ERK1/2 which are part of GPR30 signaling cascade. ERK1/2 activation in response to E2 and G1 was correlated to an increased phosphorylation of c-Jun. All treatments failed to induce these responses in the presence of EGFR inhibitor AG1478, ERK inhibitor PD98059 and ER inhibitor ICI182780. mRNA expression of cell cycle regulators cyclin A1 and B1 was downregulated by E2 and G1 while an up-regulation of proapoptotic factor Bax was observed in the same conditions. These data demonstrate that E2, working through both ER alpha and/or GPR30, activates in PS the rapid EGFR/ERK/c-Jun pathway, modulating the expression of genes involved in the balance between cellular proliferation and apoptosis. |
| doi_str_mv | 10.1016/j.mce.2010.01.035 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_hal_p</sourceid><recordid>TN_cdi_hal_primary_oai_HAL_hal_02662741v1</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>896191743</sourcerecordid><originalsourceid>FETCH-LOGICAL-h206t-53a6545af44850ce46a30cb34d5d112505b417e8ce1d0c43ead7b638b98eeb323</originalsourceid><addsrcrecordid>eNqFkU1vFDEMhiMEokvhB3BBuSEOszhxvvZYVaVFWqlV1Z5HniTdSTVfTLJb7ZF_zlQtXDnZfv34lWUz9lnAWoAw3x_XvY9rCUsNYg2o37CVcFZWDrR9y1aAgJWVYE_Yh5wfAcBq6d6zk2UEpTO4Yr-F5U0sVMVcZgpp7Dj5kg5UYuYzTSnwnHYDdWnY8YlK-0THzNNwGLtDDEuyQGVp-PZY4hB5nuLcUxn98dmApnEqY06Zl3Ye97uWX97cInAaAr-45dRNLX1k7x6oy_HTazxl9z8u7s6vqu315c_zs23VSjCl0khGK00PSjkNPipDCL5BFXQQQmrQjRI2Oh9FAK8wUrCNQddsXIwNSjxl3158W-rqaU49zcd6pFRfnW3rZw2kMdIqcRAL-_WFnebx1345Td2n7GPX0RDHfa7dxoiNsAr_S1pE4RCcXsgvr-S-6WP4t8LfX-AfEGWMQw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>733183085</pqid></control><display><type>article</type><title>17 beta-estradiol activates rapid signaling pathways involved in rat pachytene spermatocytes apoptosis through GPR30 and ER alpha</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>Chimento, Adele ; Sirianni, Rosa ; Delalande, Christelle ; Silandre, Dorothèe ; Bois, Camille ; Andò, Sebastiano ; Maggiolini, Marcello ; Carreau, Serge ; Pezzi, Vincenzo</creator><creatorcontrib>Chimento, Adele ; Sirianni, Rosa ; Delalande, Christelle ; Silandre, Dorothèe ; Bois, Camille ; Andò, Sebastiano ; Maggiolini, Marcello ; Carreau, Serge ; Pezzi, Vincenzo</creatorcontrib><description>Aim of the present study was to investigate whether estrogens were able to directly activate rapid signaling pathways controlling spermatogenesis in rat pachytene spermatocytes (PS). Classically, estrogens act by binding to estrogen receptors (ERs) alpha and beta. Recently, it has been demonstrated that rapid estrogen action can also be activated through the G-protein-coupled receptor (GPR)-30. Herein, we demonstrated that rat PS express ER alpha, ER beta and GPR30. Treatment of PS with estradiol (E2), the selective GPR30 agonist G1 and the selective ER alpha agonist PPT determined activation of ERK1/2 which are part of GPR30 signaling cascade. ERK1/2 activation in response to E2 and G1 was correlated to an increased phosphorylation of c-Jun. All treatments failed to induce these responses in the presence of EGFR inhibitor AG1478, ERK inhibitor PD98059 and ER inhibitor ICI182780. mRNA expression of cell cycle regulators cyclin A1 and B1 was downregulated by E2 and G1 while an up-regulation of proapoptotic factor Bax was observed in the same conditions. These data demonstrate that E2, working through both ER alpha and/or GPR30, activates in PS the rapid EGFR/ERK/c-Jun pathway, modulating the expression of genes involved in the balance between cellular proliferation and apoptosis.</description><identifier>ISSN: 0303-7207</identifier><identifier>EISSN: 1872-8057</identifier><identifier>EISSN: 0303-7207</identifier><identifier>DOI: 10.1016/j.mce.2010.01.035</identifier><identifier>PMID: 20132863</identifier><language>eng</language><publisher>Ireland: Elsevier</publisher><subject>Activation ; Animals ; Apoptosis ; Apoptosis - drug effects ; Cells, Cultured ; Cellular ; Computer Science ; Enzyme Activation - drug effects ; Estradiol - pharmacology ; Estrogen Receptor alpha - genetics ; Estrogen Receptor alpha - metabolism ; Estrogen Receptor beta - genetics ; Estrogen Receptor beta - metabolism ; Estrogens ; Inhibitors ; Life Sciences ; Male ; Mitogen-Activated Protein Kinase 1 - metabolism ; Mitogen-Activated Protein Kinase 3 - metabolism ; Pachytene Stage - drug effects ; Pathways ; Phenols - pharmacology ; Polystyrene resins ; Proto-Oncogene Proteins c-jun - metabolism ; Pyrazoles - pharmacology ; Rats ; Rats, Sprague-Dawley ; Receptors ; Receptors, G-Protein-Coupled - agonists ; Receptors, G-Protein-Coupled - antagonists & inhibitors ; Receptors, G-Protein-Coupled - genetics ; Receptors, G-Protein-Coupled - metabolism ; Signal Transduction - drug effects ; Spermatocytes - cytology ; Spermatocytes - drug effects ; Spermatocytes - enzymology ; Spermatocytes - metabolism</subject><ispartof>Molecular and cellular endocrinology, 2010-05, Vol.320 (1-2), p.136-144</ispartof><rights>(c) 2010 Elsevier Ireland Ltd. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20132863$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02662741$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Chimento, Adele</creatorcontrib><creatorcontrib>Sirianni, Rosa</creatorcontrib><creatorcontrib>Delalande, Christelle</creatorcontrib><creatorcontrib>Silandre, Dorothèe</creatorcontrib><creatorcontrib>Bois, Camille</creatorcontrib><creatorcontrib>Andò, Sebastiano</creatorcontrib><creatorcontrib>Maggiolini, Marcello</creatorcontrib><creatorcontrib>Carreau, Serge</creatorcontrib><creatorcontrib>Pezzi, Vincenzo</creatorcontrib><title>17 beta-estradiol activates rapid signaling pathways involved in rat pachytene spermatocytes apoptosis through GPR30 and ER alpha</title><title>Molecular and cellular endocrinology</title><addtitle>Mol Cell Endocrinol</addtitle><description>Aim of the present study was to investigate whether estrogens were able to directly activate rapid signaling pathways controlling spermatogenesis in rat pachytene spermatocytes (PS). Classically, estrogens act by binding to estrogen receptors (ERs) alpha and beta. Recently, it has been demonstrated that rapid estrogen action can also be activated through the G-protein-coupled receptor (GPR)-30. Herein, we demonstrated that rat PS express ER alpha, ER beta and GPR30. Treatment of PS with estradiol (E2), the selective GPR30 agonist G1 and the selective ER alpha agonist PPT determined activation of ERK1/2 which are part of GPR30 signaling cascade. ERK1/2 activation in response to E2 and G1 was correlated to an increased phosphorylation of c-Jun. All treatments failed to induce these responses in the presence of EGFR inhibitor AG1478, ERK inhibitor PD98059 and ER inhibitor ICI182780. mRNA expression of cell cycle regulators cyclin A1 and B1 was downregulated by E2 and G1 while an up-regulation of proapoptotic factor Bax was observed in the same conditions. These data demonstrate that E2, working through both ER alpha and/or GPR30, activates in PS the rapid EGFR/ERK/c-Jun pathway, modulating the expression of genes involved in the balance between cellular proliferation and apoptosis.</description><subject>Activation</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Cells, Cultured</subject><subject>Cellular</subject><subject>Computer Science</subject><subject>Enzyme Activation - drug effects</subject><subject>Estradiol - pharmacology</subject><subject>Estrogen Receptor alpha - genetics</subject><subject>Estrogen Receptor alpha - metabolism</subject><subject>Estrogen Receptor beta - genetics</subject><subject>Estrogen Receptor beta - metabolism</subject><subject>Estrogens</subject><subject>Inhibitors</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Mitogen-Activated Protein Kinase 1 - metabolism</subject><subject>Mitogen-Activated Protein Kinase 3 - metabolism</subject><subject>Pachytene Stage - drug effects</subject><subject>Pathways</subject><subject>Phenols - pharmacology</subject><subject>Polystyrene resins</subject><subject>Proto-Oncogene Proteins c-jun - metabolism</subject><subject>Pyrazoles - pharmacology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors</subject><subject>Receptors, G-Protein-Coupled - agonists</subject><subject>Receptors, G-Protein-Coupled - antagonists & inhibitors</subject><subject>Receptors, G-Protein-Coupled - genetics</subject><subject>Receptors, G-Protein-Coupled - metabolism</subject><subject>Signal Transduction - drug effects</subject><subject>Spermatocytes - cytology</subject><subject>Spermatocytes - drug effects</subject><subject>Spermatocytes - enzymology</subject><subject>Spermatocytes - metabolism</subject><issn>0303-7207</issn><issn>1872-8057</issn><issn>0303-7207</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1vFDEMhiMEokvhB3BBuSEOszhxvvZYVaVFWqlV1Z5HniTdSTVfTLJb7ZF_zlQtXDnZfv34lWUz9lnAWoAw3x_XvY9rCUsNYg2o37CVcFZWDrR9y1aAgJWVYE_Yh5wfAcBq6d6zk2UEpTO4Yr-F5U0sVMVcZgpp7Dj5kg5UYuYzTSnwnHYDdWnY8YlK-0THzNNwGLtDDEuyQGVp-PZY4hB5nuLcUxn98dmApnEqY06Zl3Ye97uWX97cInAaAr-45dRNLX1k7x6oy_HTazxl9z8u7s6vqu315c_zs23VSjCl0khGK00PSjkNPipDCL5BFXQQQmrQjRI2Oh9FAK8wUrCNQddsXIwNSjxl3158W-rqaU49zcd6pFRfnW3rZw2kMdIqcRAL-_WFnebx1345Td2n7GPX0RDHfa7dxoiNsAr_S1pE4RCcXsgvr-S-6WP4t8LfX-AfEGWMQw</recordid><startdate>20100514</startdate><enddate>20100514</enddate><creator>Chimento, Adele</creator><creator>Sirianni, Rosa</creator><creator>Delalande, Christelle</creator><creator>Silandre, Dorothèe</creator><creator>Bois, Camille</creator><creator>Andò, Sebastiano</creator><creator>Maggiolini, Marcello</creator><creator>Carreau, Serge</creator><creator>Pezzi, Vincenzo</creator><general>Elsevier</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>1XC</scope></search><sort><creationdate>20100514</creationdate><title>17 beta-estradiol activates rapid signaling pathways involved in rat pachytene spermatocytes apoptosis through GPR30 and ER alpha</title><author>Chimento, Adele ; Sirianni, Rosa ; Delalande, Christelle ; Silandre, Dorothèe ; Bois, Camille ; Andò, Sebastiano ; Maggiolini, Marcello ; Carreau, Serge ; Pezzi, Vincenzo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h206t-53a6545af44850ce46a30cb34d5d112505b417e8ce1d0c43ead7b638b98eeb323</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Activation</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Cells, Cultured</topic><topic>Cellular</topic><topic>Computer Science</topic><topic>Enzyme Activation - drug effects</topic><topic>Estradiol - pharmacology</topic><topic>Estrogen Receptor alpha - genetics</topic><topic>Estrogen Receptor alpha - metabolism</topic><topic>Estrogen Receptor beta - genetics</topic><topic>Estrogen Receptor beta - metabolism</topic><topic>Estrogens</topic><topic>Inhibitors</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Mitogen-Activated Protein Kinase 1 - metabolism</topic><topic>Mitogen-Activated Protein Kinase 3 - metabolism</topic><topic>Pachytene Stage - drug effects</topic><topic>Pathways</topic><topic>Phenols - pharmacology</topic><topic>Polystyrene resins</topic><topic>Proto-Oncogene Proteins c-jun - metabolism</topic><topic>Pyrazoles - pharmacology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors</topic><topic>Receptors, G-Protein-Coupled - agonists</topic><topic>Receptors, G-Protein-Coupled - antagonists & inhibitors</topic><topic>Receptors, G-Protein-Coupled - genetics</topic><topic>Receptors, G-Protein-Coupled - metabolism</topic><topic>Signal Transduction - drug effects</topic><topic>Spermatocytes - cytology</topic><topic>Spermatocytes - drug effects</topic><topic>Spermatocytes - enzymology</topic><topic>Spermatocytes - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chimento, Adele</creatorcontrib><creatorcontrib>Sirianni, Rosa</creatorcontrib><creatorcontrib>Delalande, Christelle</creatorcontrib><creatorcontrib>Silandre, Dorothèe</creatorcontrib><creatorcontrib>Bois, Camille</creatorcontrib><creatorcontrib>Andò, Sebastiano</creatorcontrib><creatorcontrib>Maggiolini, Marcello</creatorcontrib><creatorcontrib>Carreau, Serge</creatorcontrib><creatorcontrib>Pezzi, Vincenzo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Molecular and cellular endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chimento, Adele</au><au>Sirianni, Rosa</au><au>Delalande, Christelle</au><au>Silandre, Dorothèe</au><au>Bois, Camille</au><au>Andò, Sebastiano</au><au>Maggiolini, Marcello</au><au>Carreau, Serge</au><au>Pezzi, Vincenzo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>17 beta-estradiol activates rapid signaling pathways involved in rat pachytene spermatocytes apoptosis through GPR30 and ER alpha</atitle><jtitle>Molecular and cellular endocrinology</jtitle><addtitle>Mol Cell Endocrinol</addtitle><date>2010-05-14</date><risdate>2010</risdate><volume>320</volume><issue>1-2</issue><spage>136</spage><epage>144</epage><pages>136-144</pages><issn>0303-7207</issn><eissn>1872-8057</eissn><eissn>0303-7207</eissn><abstract>Aim of the present study was to investigate whether estrogens were able to directly activate rapid signaling pathways controlling spermatogenesis in rat pachytene spermatocytes (PS). Classically, estrogens act by binding to estrogen receptors (ERs) alpha and beta. Recently, it has been demonstrated that rapid estrogen action can also be activated through the G-protein-coupled receptor (GPR)-30. Herein, we demonstrated that rat PS express ER alpha, ER beta and GPR30. Treatment of PS with estradiol (E2), the selective GPR30 agonist G1 and the selective ER alpha agonist PPT determined activation of ERK1/2 which are part of GPR30 signaling cascade. ERK1/2 activation in response to E2 and G1 was correlated to an increased phosphorylation of c-Jun. All treatments failed to induce these responses in the presence of EGFR inhibitor AG1478, ERK inhibitor PD98059 and ER inhibitor ICI182780. mRNA expression of cell cycle regulators cyclin A1 and B1 was downregulated by E2 and G1 while an up-regulation of proapoptotic factor Bax was observed in the same conditions. These data demonstrate that E2, working through both ER alpha and/or GPR30, activates in PS the rapid EGFR/ERK/c-Jun pathway, modulating the expression of genes involved in the balance between cellular proliferation and apoptosis.</abstract><cop>Ireland</cop><pub>Elsevier</pub><pmid>20132863</pmid><doi>10.1016/j.mce.2010.01.035</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0303-7207 |
| ispartof | Molecular and cellular endocrinology, 2010-05, Vol.320 (1-2), p.136-144 |
| issn | 0303-7207 1872-8057 0303-7207 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_02662741v1 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Activation Animals Apoptosis Apoptosis - drug effects Cells, Cultured Cellular Computer Science Enzyme Activation - drug effects Estradiol - pharmacology Estrogen Receptor alpha - genetics Estrogen Receptor alpha - metabolism Estrogen Receptor beta - genetics Estrogen Receptor beta - metabolism Estrogens Inhibitors Life Sciences Male Mitogen-Activated Protein Kinase 1 - metabolism Mitogen-Activated Protein Kinase 3 - metabolism Pachytene Stage - drug effects Pathways Phenols - pharmacology Polystyrene resins Proto-Oncogene Proteins c-jun - metabolism Pyrazoles - pharmacology Rats Rats, Sprague-Dawley Receptors Receptors, G-Protein-Coupled - agonists Receptors, G-Protein-Coupled - antagonists & inhibitors Receptors, G-Protein-Coupled - genetics Receptors, G-Protein-Coupled - metabolism Signal Transduction - drug effects Spermatocytes - cytology Spermatocytes - drug effects Spermatocytes - enzymology Spermatocytes - metabolism |
| title | 17 beta-estradiol activates rapid signaling pathways involved in rat pachytene spermatocytes apoptosis through GPR30 and ER alpha |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T09%3A45%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_hal_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=17%20beta-estradiol%20activates%20rapid%20signaling%20pathways%20involved%20in%20rat%20pachytene%20spermatocytes%20apoptosis%20through%20GPR30%20and%20ER%20alpha&rft.jtitle=Molecular%20and%20cellular%20endocrinology&rft.au=Chimento,%20Adele&rft.date=2010-05-14&rft.volume=320&rft.issue=1-2&rft.spage=136&rft.epage=144&rft.pages=136-144&rft.issn=0303-7207&rft.eissn=1872-8057&rft_id=info:doi/10.1016/j.mce.2010.01.035&rft_dat=%3Cproquest_hal_p%3E896191743%3C/proquest_hal_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=733183085&rft_id=info:pmid/20132863&rfr_iscdi=true |