Effects of abomasal lipid infusion on liver triglyceride accumulation and adipose lipolysis during fatty liver induction in dairy cows
The objective was to determine the effects of abomasal infusion of linseed oil on liver triglyceride (TG) accumulation and adipose tissue lipolysis during an experimental protocol for induction of fatty liver. Eight nonpregnant, nonlactating Holstein cows were randomly assigned to treatments in a re...
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| Veröffentlicht in: | Journal of dairy science 2009-10, Vol.92 (10), p.4954-4961 |
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| description | The objective was to determine the effects of abomasal infusion of linseed oil on liver triglyceride (TG) accumulation and adipose tissue lipolysis during an experimental protocol for induction of fatty liver. Eight nonpregnant, nonlactating Holstein cows were randomly assigned to treatments in a replicated 4×4 Latin square design. Treatments were abomasal infusion of water (W), tallow (T), linseed oil (LO), or half linseed oil and half tallow (LOT) at a rate of 0.56g/kg of body weight per day. Each experimental period consisted of a 4-d fast concurrent with administration of treatments into the abomasum in 6 equal doses per day (every 4h). Cows were fed ad libitum for 24 d between periods of fasting and lipid infusion. Infusion of linseed oil (LO and LOT) increased α-linolenic acid (C18:3n-3) content in serum (12.2, 10.4, 4.2, and 4.6g/100g of fatty acids for LO, LOT, T, and W, respectively), but not in the nonesterified fatty acid (NEFA) fraction of plasma. Treatments had no effect on plasma NEFA concentrations. Abomasal infusion of lipid increased in vitro stimulated lipolysis in subcutaneous adipose tissue, compared with W (4,294, 3,809, 4,231, and 3,293 nmol of glycerol released×g−1 tissue×2h−1 for LO, LOT, T, and W, respectively), but there was no difference between fat sources. Hepatic TG accumulation over 4-d fast was 2.52, 2.60, 2.64, and 2.09±0.75μg of TG/μg of DNA for W, LO, LOT, and T, respectively, which did not differ. Abomasal infusion of LO did not reduce liver TG accumulation, plasma NEFA concentration, or alter in vitro adipose tissue lipolysis when compared with T. These results contrast with a previous study involving i.v. infusion of lipid emulsion derived from LO. Discrepancies might be explained by the use of different administration routes and a relatively modest induction of liver TG accumulation in the current experiment. |
| doi_str_mv | 10.3168/jds.2009-2147 |
| format | Article |
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Eight nonpregnant, nonlactating Holstein cows were randomly assigned to treatments in a replicated 4×4 Latin square design. Treatments were abomasal infusion of water (W), tallow (T), linseed oil (LO), or half linseed oil and half tallow (LOT) at a rate of 0.56g/kg of body weight per day. Each experimental period consisted of a 4-d fast concurrent with administration of treatments into the abomasum in 6 equal doses per day (every 4h). Cows were fed ad libitum for 24 d between periods of fasting and lipid infusion. Infusion of linseed oil (LO and LOT) increased α-linolenic acid (C18:3n-3) content in serum (12.2, 10.4, 4.2, and 4.6g/100g of fatty acids for LO, LOT, T, and W, respectively), but not in the nonesterified fatty acid (NEFA) fraction of plasma. Treatments had no effect on plasma NEFA concentrations. Abomasal infusion of lipid increased in vitro stimulated lipolysis in subcutaneous adipose tissue, compared with W (4,294, 3,809, 4,231, and 3,293 nmol of glycerol released×g−1 tissue×2h−1 for LO, LOT, T, and W, respectively), but there was no difference between fat sources. Hepatic TG accumulation over 4-d fast was 2.52, 2.60, 2.64, and 2.09±0.75μg of TG/μg of DNA for W, LO, LOT, and T, respectively, which did not differ. Abomasal infusion of LO did not reduce liver TG accumulation, plasma NEFA concentration, or alter in vitro adipose tissue lipolysis when compared with T. These results contrast with a previous study involving i.v. infusion of lipid emulsion derived from LO. Discrepancies might be explained by the use of different administration routes and a relatively modest induction of liver TG accumulation in the current experiment.</description><identifier>ISSN: 0022-0302</identifier><identifier>EISSN: 1525-3198</identifier><identifier>DOI: 10.3168/jds.2009-2147</identifier><identifier>PMID: 19762812</identifier><identifier>CODEN: JDSCAE</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>abomasal lipid infusion ; abomasum ; Abomasum - drug effects ; adipose tissue ; Adipose Tissue - metabolism ; Agricultural sciences ; alpha-Linolenic Acid - analysis ; alpha-Linolenic Acid - blood ; Animal productions ; Animals ; application methods ; Biological and medical sciences ; bovine ; Cattle ; cattle diseases ; dairy cows ; Fats - administration & dosage ; Fats - chemistry ; Fatty Acids - analysis ; Fatty Acids - blood ; Fatty Acids, Nonesterified - blood ; fatty liver ; Fatty Liver - chemically induced ; Fatty Liver - veterinary ; Female ; Food industries ; Fundamental and applied biological sciences. Psychology ; In Vitro Techniques ; Life Sciences ; linseed oil ; Linseed Oil - administration & dosage ; Linseed Oil - chemistry ; lipids ; lipolysis ; Lipolysis - drug effects ; liver ; Liver - chemistry ; Liver - drug effects ; Liver - metabolism ; liver triglyceride ; Milk and cheese industries. Ice creams ; Subcutaneous Fat - metabolism ; Terrestrial animal productions ; therapeutics ; triacylglycerols ; Triglycerides - analysis ; Triglycerides - metabolism ; Vertebrates</subject><ispartof>Journal of dairy science, 2009-10, Vol.92 (10), p.4954-4961</ispartof><rights>2009 American Dairy Science Association</rights><rights>2009 INIST-CNRS</rights><rights>Copyright American Dairy Science Association Oct 2009</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c526t-18ac7c4f529b68b2876fdfd60cc71d3100c93084abd4c9c77341b1085e747ea93</citedby><cites>FETCH-LOGICAL-c526t-18ac7c4f529b68b2876fdfd60cc71d3100c93084abd4c9c77341b1085e747ea93</cites><orcidid>0000-0002-3773-9293</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.3168/jds.2009-2147$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21965678$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19762812$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02662205$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Brickner, A.E.</creatorcontrib><creatorcontrib>Pires, J.A.A.</creatorcontrib><creatorcontrib>Gressley, T.F.</creatorcontrib><creatorcontrib>Grummer, R.R.</creatorcontrib><title>Effects of abomasal lipid infusion on liver triglyceride accumulation and adipose lipolysis during fatty liver induction in dairy cows</title><title>Journal of dairy science</title><addtitle>J Dairy Sci</addtitle><description>The objective was to determine the effects of abomasal infusion of linseed oil on liver triglyceride (TG) accumulation and adipose tissue lipolysis during an experimental protocol for induction of fatty liver. Eight nonpregnant, nonlactating Holstein cows were randomly assigned to treatments in a replicated 4×4 Latin square design. Treatments were abomasal infusion of water (W), tallow (T), linseed oil (LO), or half linseed oil and half tallow (LOT) at a rate of 0.56g/kg of body weight per day. Each experimental period consisted of a 4-d fast concurrent with administration of treatments into the abomasum in 6 equal doses per day (every 4h). Cows were fed ad libitum for 24 d between periods of fasting and lipid infusion. Infusion of linseed oil (LO and LOT) increased α-linolenic acid (C18:3n-3) content in serum (12.2, 10.4, 4.2, and 4.6g/100g of fatty acids for LO, LOT, T, and W, respectively), but not in the nonesterified fatty acid (NEFA) fraction of plasma. Treatments had no effect on plasma NEFA concentrations. Abomasal infusion of lipid increased in vitro stimulated lipolysis in subcutaneous adipose tissue, compared with W (4,294, 3,809, 4,231, and 3,293 nmol of glycerol released×g−1 tissue×2h−1 for LO, LOT, T, and W, respectively), but there was no difference between fat sources. Hepatic TG accumulation over 4-d fast was 2.52, 2.60, 2.64, and 2.09±0.75μg of TG/μg of DNA for W, LO, LOT, and T, respectively, which did not differ. Abomasal infusion of LO did not reduce liver TG accumulation, plasma NEFA concentration, or alter in vitro adipose tissue lipolysis when compared with T. These results contrast with a previous study involving i.v. infusion of lipid emulsion derived from LO. Discrepancies might be explained by the use of different administration routes and a relatively modest induction of liver TG accumulation in the current experiment.</description><subject>abomasal lipid infusion</subject><subject>abomasum</subject><subject>Abomasum - drug effects</subject><subject>adipose tissue</subject><subject>Adipose Tissue - metabolism</subject><subject>Agricultural sciences</subject><subject>alpha-Linolenic Acid - analysis</subject><subject>alpha-Linolenic Acid - blood</subject><subject>Animal productions</subject><subject>Animals</subject><subject>application methods</subject><subject>Biological and medical sciences</subject><subject>bovine</subject><subject>Cattle</subject><subject>cattle diseases</subject><subject>dairy cows</subject><subject>Fats - administration & dosage</subject><subject>Fats - chemistry</subject><subject>Fatty Acids - analysis</subject><subject>Fatty Acids - blood</subject><subject>Fatty Acids, Nonesterified - blood</subject><subject>fatty liver</subject><subject>Fatty Liver - chemically induced</subject><subject>Fatty Liver - veterinary</subject><subject>Female</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Vitro Techniques</subject><subject>Life Sciences</subject><subject>linseed oil</subject><subject>Linseed Oil - administration & dosage</subject><subject>Linseed Oil - chemistry</subject><subject>lipids</subject><subject>lipolysis</subject><subject>Lipolysis - drug effects</subject><subject>liver</subject><subject>Liver - chemistry</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>liver triglyceride</subject><subject>Milk and cheese industries. Ice creams</subject><subject>Subcutaneous Fat - metabolism</subject><subject>Terrestrial animal productions</subject><subject>therapeutics</subject><subject>triacylglycerols</subject><subject>Triglycerides - analysis</subject><subject>Triglycerides - metabolism</subject><subject>Vertebrates</subject><issn>0022-0302</issn><issn>1525-3198</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp10U2L1DAYB_Aiiju7evSqYWERD12T9CXJcVlWVxjwoHsOT_MymyHTjEk7S7-An9vUlvUkFErLL_88yb8o3hF8XZGWf97rdE0xFiUlNXtRbEhDm7Iigr8sNhhTWuIK07PiPKV9_iQUN6-LMyJYSzmhm-L3nbVGDQkFi6ALB0jgkXdHp5Hr7Zhc6FF-vDuZiIbodn5SJjptECg1HkYPw0yg1wi0O4Zk5tXBT8klpMfo-h2yMAzTGuF6Paq_S1yPNLg4IRWe0pvilQWfzNv1fVE8fLn7eXtfbr9__XZ7sy1VQ9uhJBwUU7VtqOha3lHOWqutbrFSjOiKYKxEhXkNna6VUIxVNekI5o1hNTMgqovi05L7CF4eoztAnGQAJ-9vtnL-h2nb0nxJJ5Lt5WKPMfwaTRrkPoyxz-NJIhqeHacZlQtSMaQUjX1OJVjOBclckJwLknNB2b9fQ8fuYPQ_vTaSwdUKICnwNkKvXHp2lIi2aRnP7uN6Erd7fHLRyHQA73MsmbcUdB6gFk2d5YdFWggSdjGnPfygmFQ4j4drUmXBFmHy1Z-ciTIpZ3pldM5Vg9TB_ec4fwDnJMMy</recordid><startdate>20091001</startdate><enddate>20091001</enddate><creator>Brickner, A.E.</creator><creator>Pires, J.A.A.</creator><creator>Gressley, T.F.</creator><creator>Grummer, R.R.</creator><general>Elsevier Inc</general><general>American Dairy Science Association</general><general>Am Dairy Sci Assoc</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7S</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>S0X</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-3773-9293</orcidid></search><sort><creationdate>20091001</creationdate><title>Effects of abomasal lipid infusion on liver triglyceride accumulation and adipose lipolysis during fatty liver induction in dairy cows</title><author>Brickner, A.E. ; Pires, J.A.A. ; Gressley, T.F. ; Grummer, R.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c526t-18ac7c4f529b68b2876fdfd60cc71d3100c93084abd4c9c77341b1085e747ea93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>abomasal lipid infusion</topic><topic>abomasum</topic><topic>Abomasum - drug effects</topic><topic>adipose tissue</topic><topic>Adipose Tissue - metabolism</topic><topic>Agricultural sciences</topic><topic>alpha-Linolenic Acid - analysis</topic><topic>alpha-Linolenic Acid - blood</topic><topic>Animal productions</topic><topic>Animals</topic><topic>application methods</topic><topic>Biological and medical sciences</topic><topic>bovine</topic><topic>Cattle</topic><topic>cattle diseases</topic><topic>dairy cows</topic><topic>Fats - administration & dosage</topic><topic>Fats - chemistry</topic><topic>Fatty Acids - analysis</topic><topic>Fatty Acids - blood</topic><topic>Fatty Acids, Nonesterified - blood</topic><topic>fatty liver</topic><topic>Fatty Liver - chemically induced</topic><topic>Fatty Liver - veterinary</topic><topic>Female</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Vitro Techniques</topic><topic>Life Sciences</topic><topic>linseed oil</topic><topic>Linseed Oil - administration & dosage</topic><topic>Linseed Oil - chemistry</topic><topic>lipids</topic><topic>lipolysis</topic><topic>Lipolysis - drug effects</topic><topic>liver</topic><topic>Liver - chemistry</topic><topic>Liver - drug effects</topic><topic>Liver - metabolism</topic><topic>liver triglyceride</topic><topic>Milk and cheese industries. Ice creams</topic><topic>Subcutaneous Fat - metabolism</topic><topic>Terrestrial animal productions</topic><topic>therapeutics</topic><topic>triacylglycerols</topic><topic>Triglycerides - analysis</topic><topic>Triglycerides - metabolism</topic><topic>Vertebrates</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brickner, A.E.</creatorcontrib><creatorcontrib>Pires, J.A.A.</creatorcontrib><creatorcontrib>Gressley, T.F.</creatorcontrib><creatorcontrib>Grummer, R.R.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Engineering Database</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>SIRS Editorial</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of dairy science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brickner, A.E.</au><au>Pires, J.A.A.</au><au>Gressley, T.F.</au><au>Grummer, R.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of abomasal lipid infusion on liver triglyceride accumulation and adipose lipolysis during fatty liver induction in dairy cows</atitle><jtitle>Journal of dairy science</jtitle><addtitle>J Dairy Sci</addtitle><date>2009-10-01</date><risdate>2009</risdate><volume>92</volume><issue>10</issue><spage>4954</spage><epage>4961</epage><pages>4954-4961</pages><issn>0022-0302</issn><eissn>1525-3198</eissn><coden>JDSCAE</coden><abstract>The objective was to determine the effects of abomasal infusion of linseed oil on liver triglyceride (TG) accumulation and adipose tissue lipolysis during an experimental protocol for induction of fatty liver. Eight nonpregnant, nonlactating Holstein cows were randomly assigned to treatments in a replicated 4×4 Latin square design. Treatments were abomasal infusion of water (W), tallow (T), linseed oil (LO), or half linseed oil and half tallow (LOT) at a rate of 0.56g/kg of body weight per day. Each experimental period consisted of a 4-d fast concurrent with administration of treatments into the abomasum in 6 equal doses per day (every 4h). Cows were fed ad libitum for 24 d between periods of fasting and lipid infusion. Infusion of linseed oil (LO and LOT) increased α-linolenic acid (C18:3n-3) content in serum (12.2, 10.4, 4.2, and 4.6g/100g of fatty acids for LO, LOT, T, and W, respectively), but not in the nonesterified fatty acid (NEFA) fraction of plasma. Treatments had no effect on plasma NEFA concentrations. Abomasal infusion of lipid increased in vitro stimulated lipolysis in subcutaneous adipose tissue, compared with W (4,294, 3,809, 4,231, and 3,293 nmol of glycerol released×g−1 tissue×2h−1 for LO, LOT, T, and W, respectively), but there was no difference between fat sources. Hepatic TG accumulation over 4-d fast was 2.52, 2.60, 2.64, and 2.09±0.75μg of TG/μg of DNA for W, LO, LOT, and T, respectively, which did not differ. Abomasal infusion of LO did not reduce liver TG accumulation, plasma NEFA concentration, or alter in vitro adipose tissue lipolysis when compared with T. These results contrast with a previous study involving i.v. infusion of lipid emulsion derived from LO. Discrepancies might be explained by the use of different administration routes and a relatively modest induction of liver TG accumulation in the current experiment.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>19762812</pmid><doi>10.3168/jds.2009-2147</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-3773-9293</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of dairy science, 2009-10, Vol.92 (10), p.4954-4961 |
| issn | 0022-0302 1525-3198 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | abomasal lipid infusion abomasum Abomasum - drug effects adipose tissue Adipose Tissue - metabolism Agricultural sciences alpha-Linolenic Acid - analysis alpha-Linolenic Acid - blood Animal productions Animals application methods Biological and medical sciences bovine Cattle cattle diseases dairy cows Fats - administration & dosage Fats - chemistry Fatty Acids - analysis Fatty Acids - blood Fatty Acids, Nonesterified - blood fatty liver Fatty Liver - chemically induced Fatty Liver - veterinary Female Food industries Fundamental and applied biological sciences. Psychology In Vitro Techniques Life Sciences linseed oil Linseed Oil - administration & dosage Linseed Oil - chemistry lipids lipolysis Lipolysis - drug effects liver Liver - chemistry Liver - drug effects Liver - metabolism liver triglyceride Milk and cheese industries. Ice creams Subcutaneous Fat - metabolism Terrestrial animal productions therapeutics triacylglycerols Triglycerides - analysis Triglycerides - metabolism Vertebrates |
| title | Effects of abomasal lipid infusion on liver triglyceride accumulation and adipose lipolysis during fatty liver induction in dairy cows |
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