Effects of Illicit Dexamethasone upon Hepatic Drug Metabolizing Enzymes and Related Transcription Factors mRNAs and Their Potential Use As Biomarkers in Cattle
In cattle fattening, the illicit use of growth promoters (GPs) represents a major problem. The synthetic corticosteroid dexamethasone (DEX) is the GP mostly used, alone or in combination with other steroids or β-agonists. Recently, GPs were shown to disrupt some cattle cytochromes P450 (CYPs) at the...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2010-01, Vol.58 (2), p.1342-1349 |
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| creator | Giantin, Mery Lopparelli, Rosa M Zancanella, Vanessa Martin, Pascal G Polizzi, Arnaud Gallina, Guglielmo Gottardo, Flaviana Montesissa, Clara Ravarotto, Licia Pineau, Thierry Dacasto, Mauro |
| description | In cattle fattening, the illicit use of growth promoters (GPs) represents a major problem. The synthetic corticosteroid dexamethasone (DEX) is the GP mostly used, alone or in combination with other steroids or β-agonists. Recently, GPs were shown to disrupt some cattle cytochromes P450 (CYPs) at the post-transcriptional level; therefore, the effects of two illicit protocols containing DEX (alone or together with 17β-estradiol, 17βE) upon main cattle liver drug metabolizing enzymes (DMEs) mRNAs and related transcription factors were investigated by quantitative real time RT-PCR. Eleven genes, out of the 18 considered, were significantly modulated by GPs. Corticosteroid-responsive genes did not respond univocally, whereas retinoic X receptor alpha (RXRα) and estrogen receptor alpha (ERα) were upregulated depending on the illicit protocol used. Nowadays, an increasing interest has been noticed toward the detection of biomarkers of response (BMRs) to be used in the screening of GPs misuse in cattle farming. In the present study, CYP2B6-like, CYP2E1, glutathione S-transferase A1- and sulfotransferase A1-like (GSTA1- and SULT1A1-like) mRNAs were significantly modulated regardless of the GP, the illicit protocol, and the animal breed, representing promising BMRs. The usefulness of these BMRs needs to be characterized more in depth. |
| doi_str_mv | 10.1021/jf9033317 |
| format | Article |
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The synthetic corticosteroid dexamethasone (DEX) is the GP mostly used, alone or in combination with other steroids or β-agonists. Recently, GPs were shown to disrupt some cattle cytochromes P450 (CYPs) at the post-transcriptional level; therefore, the effects of two illicit protocols containing DEX (alone or together with 17β-estradiol, 17βE) upon main cattle liver drug metabolizing enzymes (DMEs) mRNAs and related transcription factors were investigated by quantitative real time RT-PCR. Eleven genes, out of the 18 considered, were significantly modulated by GPs. Corticosteroid-responsive genes did not respond univocally, whereas retinoic X receptor alpha (RXRα) and estrogen receptor alpha (ERα) were upregulated depending on the illicit protocol used. Nowadays, an increasing interest has been noticed toward the detection of biomarkers of response (BMRs) to be used in the screening of GPs misuse in cattle farming. In the present study, CYP2B6-like, CYP2E1, glutathione S-transferase A1- and sulfotransferase A1-like (GSTA1- and SULT1A1-like) mRNAs were significantly modulated regardless of the GP, the illicit protocol, and the animal breed, representing promising BMRs. The usefulness of these BMRs needs to be characterized more in depth.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf9033317</identifier><identifier>PMID: 20041653</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Animals ; Biological and medical sciences ; Biomarkers - analysis ; Biomarkers - metabolism ; Cattle - genetics ; Cattle - growth & development ; Cattle - metabolism ; Cytochrome P-450 Enzyme System - genetics ; Cytochrome P-450 Enzyme System - metabolism ; Dexamethasone - administration & dosage ; Dexamethasone - metabolism ; Food engineering ; Food industries ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation - drug effects ; Glutathione Transferase - genetics ; Glutathione Transferase - metabolism ; Life Sciences ; Liver - chemistry ; Liver - drug effects ; Liver - enzymology ; Liver - metabolism ; Male ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Substance Abuse Detection - methods ; Toxicology in Agriculture and Food ; Transcription Factors - genetics ; Transcription Factors - metabolism</subject><ispartof>Journal of agricultural and food chemistry, 2010-01, Vol.58 (2), p.1342-1349</ispartof><rights>Copyright © 2009 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a378t-fd7c11bd45201967fece48d1d330370017ed70dabd63a589864158e095895d33</citedby><orcidid>0000-0003-1986-2770 ; 0000-0002-4271-658X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf9033317$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf9033317$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>230,314,776,780,881,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22363861$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20041653$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02660372$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Giantin, Mery</creatorcontrib><creatorcontrib>Lopparelli, Rosa M</creatorcontrib><creatorcontrib>Zancanella, Vanessa</creatorcontrib><creatorcontrib>Martin, Pascal G</creatorcontrib><creatorcontrib>Polizzi, Arnaud</creatorcontrib><creatorcontrib>Gallina, Guglielmo</creatorcontrib><creatorcontrib>Gottardo, Flaviana</creatorcontrib><creatorcontrib>Montesissa, Clara</creatorcontrib><creatorcontrib>Ravarotto, Licia</creatorcontrib><creatorcontrib>Pineau, Thierry</creatorcontrib><creatorcontrib>Dacasto, Mauro</creatorcontrib><title>Effects of Illicit Dexamethasone upon Hepatic Drug Metabolizing Enzymes and Related Transcription Factors mRNAs and Their Potential Use As Biomarkers in Cattle</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>In cattle fattening, the illicit use of growth promoters (GPs) represents a major problem. The synthetic corticosteroid dexamethasone (DEX) is the GP mostly used, alone or in combination with other steroids or β-agonists. Recently, GPs were shown to disrupt some cattle cytochromes P450 (CYPs) at the post-transcriptional level; therefore, the effects of two illicit protocols containing DEX (alone or together with 17β-estradiol, 17βE) upon main cattle liver drug metabolizing enzymes (DMEs) mRNAs and related transcription factors were investigated by quantitative real time RT-PCR. Eleven genes, out of the 18 considered, were significantly modulated by GPs. Corticosteroid-responsive genes did not respond univocally, whereas retinoic X receptor alpha (RXRα) and estrogen receptor alpha (ERα) were upregulated depending on the illicit protocol used. Nowadays, an increasing interest has been noticed toward the detection of biomarkers of response (BMRs) to be used in the screening of GPs misuse in cattle farming. In the present study, CYP2B6-like, CYP2E1, glutathione S-transferase A1- and sulfotransferase A1-like (GSTA1- and SULT1A1-like) mRNAs were significantly modulated regardless of the GP, the illicit protocol, and the animal breed, representing promising BMRs. The usefulness of these BMRs needs to be characterized more in depth.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - analysis</subject><subject>Biomarkers - metabolism</subject><subject>Cattle - genetics</subject><subject>Cattle - growth & development</subject><subject>Cattle - metabolism</subject><subject>Cytochrome P-450 Enzyme System - genetics</subject><subject>Cytochrome P-450 Enzyme System - metabolism</subject><subject>Dexamethasone - administration & dosage</subject><subject>Dexamethasone - metabolism</subject><subject>Food engineering</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Glutathione Transferase - genetics</subject><subject>Glutathione Transferase - metabolism</subject><subject>Life Sciences</subject><subject>Liver - chemistry</subject><subject>Liver - drug effects</subject><subject>Liver - enzymology</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Substance Abuse Detection - methods</subject><subject>Toxicology in Agriculture and Food</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkctuEzEUhkcIRNPCghdA3iDUxYA9nouzDGlKKoWLqrAendhnGgePPbU9iPZleFVcJSQbVrZ8Pn_2OX-WvWH0A6MF-7jrppRzzppn2YRVBc0rxsTzbEJTMRdVzc6y8xB2lFJRNfRldlZQWrK64pPsz6LrUMZAXEdujNFSR3KFv6HHuIXgLJJxcJYscYCoJbny4x35ghE2zuhHbe_Iwj4-9BgIWEVu0UBERdYebJBeD1Gnu9cgo_OB9LdfZ3tuvUXtyXcX0UYNhvwISFLpk3Y9-J-YWG3JHGI0-Cp70YEJ-PqwXmTr68V6vsxX3z7fzGerHHgjYt6pRjK2UWXqnk3rJvWEpVBMcU55QylrUDVUwUbVHCoxFXXJKoF0mvZVgi6yy712C6YdvE7_eGgd6HY5W7VPZ7So62QqfrHEvt-zg3f3I4bY9jpINAYsujG0DeeCNrQsT1bpXQgeu6Oa0fYpufaYXGLfHqzjpkd1JP9FlYB3BwCCBNOlGUsdTlzBay5qduJAhnbnRm_T3P7z4F_67qu9</recordid><startdate>20100127</startdate><enddate>20100127</enddate><creator>Giantin, Mery</creator><creator>Lopparelli, Rosa M</creator><creator>Zancanella, Vanessa</creator><creator>Martin, Pascal G</creator><creator>Polizzi, Arnaud</creator><creator>Gallina, Guglielmo</creator><creator>Gottardo, Flaviana</creator><creator>Montesissa, Clara</creator><creator>Ravarotto, Licia</creator><creator>Pineau, Thierry</creator><creator>Dacasto, Mauro</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-1986-2770</orcidid><orcidid>https://orcid.org/0000-0002-4271-658X</orcidid></search><sort><creationdate>20100127</creationdate><title>Effects of Illicit Dexamethasone upon Hepatic Drug Metabolizing Enzymes and Related Transcription Factors mRNAs and Their Potential Use As Biomarkers in Cattle</title><author>Giantin, Mery ; Lopparelli, Rosa M ; Zancanella, Vanessa ; Martin, Pascal G ; Polizzi, Arnaud ; Gallina, Guglielmo ; Gottardo, Flaviana ; Montesissa, Clara ; Ravarotto, Licia ; Pineau, Thierry ; Dacasto, Mauro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a378t-fd7c11bd45201967fece48d1d330370017ed70dabd63a589864158e095895d33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - analysis</topic><topic>Biomarkers - metabolism</topic><topic>Cattle - genetics</topic><topic>Cattle - growth & development</topic><topic>Cattle - metabolism</topic><topic>Cytochrome P-450 Enzyme System - genetics</topic><topic>Cytochrome P-450 Enzyme System - metabolism</topic><topic>Dexamethasone - administration & dosage</topic><topic>Dexamethasone - metabolism</topic><topic>Food engineering</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Glutathione Transferase - genetics</topic><topic>Glutathione Transferase - metabolism</topic><topic>Life Sciences</topic><topic>Liver - chemistry</topic><topic>Liver - drug effects</topic><topic>Liver - enzymology</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Substance Abuse Detection - methods</topic><topic>Toxicology in Agriculture and Food</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Giantin, Mery</creatorcontrib><creatorcontrib>Lopparelli, Rosa M</creatorcontrib><creatorcontrib>Zancanella, Vanessa</creatorcontrib><creatorcontrib>Martin, Pascal G</creatorcontrib><creatorcontrib>Polizzi, Arnaud</creatorcontrib><creatorcontrib>Gallina, Guglielmo</creatorcontrib><creatorcontrib>Gottardo, Flaviana</creatorcontrib><creatorcontrib>Montesissa, Clara</creatorcontrib><creatorcontrib>Ravarotto, Licia</creatorcontrib><creatorcontrib>Pineau, Thierry</creatorcontrib><creatorcontrib>Dacasto, Mauro</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Giantin, Mery</au><au>Lopparelli, Rosa M</au><au>Zancanella, Vanessa</au><au>Martin, Pascal G</au><au>Polizzi, Arnaud</au><au>Gallina, Guglielmo</au><au>Gottardo, Flaviana</au><au>Montesissa, Clara</au><au>Ravarotto, Licia</au><au>Pineau, Thierry</au><au>Dacasto, Mauro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Illicit Dexamethasone upon Hepatic Drug Metabolizing Enzymes and Related Transcription Factors mRNAs and Their Potential Use As Biomarkers in Cattle</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2010-01-27</date><risdate>2010</risdate><volume>58</volume><issue>2</issue><spage>1342</spage><epage>1349</epage><pages>1342-1349</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>In cattle fattening, the illicit use of growth promoters (GPs) represents a major problem. The synthetic corticosteroid dexamethasone (DEX) is the GP mostly used, alone or in combination with other steroids or β-agonists. Recently, GPs were shown to disrupt some cattle cytochromes P450 (CYPs) at the post-transcriptional level; therefore, the effects of two illicit protocols containing DEX (alone or together with 17β-estradiol, 17βE) upon main cattle liver drug metabolizing enzymes (DMEs) mRNAs and related transcription factors were investigated by quantitative real time RT-PCR. Eleven genes, out of the 18 considered, were significantly modulated by GPs. Corticosteroid-responsive genes did not respond univocally, whereas retinoic X receptor alpha (RXRα) and estrogen receptor alpha (ERα) were upregulated depending on the illicit protocol used. Nowadays, an increasing interest has been noticed toward the detection of biomarkers of response (BMRs) to be used in the screening of GPs misuse in cattle farming. In the present study, CYP2B6-like, CYP2E1, glutathione S-transferase A1- and sulfotransferase A1-like (GSTA1- and SULT1A1-like) mRNAs were significantly modulated regardless of the GP, the illicit protocol, and the animal breed, representing promising BMRs. The usefulness of these BMRs needs to be characterized more in depth.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>20041653</pmid><doi>10.1021/jf9033317</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-1986-2770</orcidid><orcidid>https://orcid.org/0000-0002-4271-658X</orcidid></addata></record> |
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| subjects | Animals Biological and medical sciences Biomarkers - analysis Biomarkers - metabolism Cattle - genetics Cattle - growth & development Cattle - metabolism Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - metabolism Dexamethasone - administration & dosage Dexamethasone - metabolism Food engineering Food industries Fundamental and applied biological sciences. Psychology Gene Expression Regulation - drug effects Glutathione Transferase - genetics Glutathione Transferase - metabolism Life Sciences Liver - chemistry Liver - drug effects Liver - enzymology Liver - metabolism Male RNA, Messenger - genetics RNA, Messenger - metabolism Substance Abuse Detection - methods Toxicology in Agriculture and Food Transcription Factors - genetics Transcription Factors - metabolism |
| title | Effects of Illicit Dexamethasone upon Hepatic Drug Metabolizing Enzymes and Related Transcription Factors mRNAs and Their Potential Use As Biomarkers in Cattle |
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