Development of a method to determine carotenoid composition of fresh forages
Due to the limited interest in carotenoids in ruminant diets until recently, analyses of forages are often incomplete, focusing mainly on β-carotene and lutein. Carotenoid composition of green forage from middle mountain meadow was analyzed by HPLC after extraction and elimination of chlorophylls by...
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| Veröffentlicht in: | Canadian journal of plant science 2008, Vol.88 (6), p.1057-1064 |
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| container_issue | 6 |
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| container_title | Canadian journal of plant science |
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| creator | Cardinault, N Lyan, B Doreau, M Chauveau, B Rock, E Grolier, P |
| description | Due to the limited interest in carotenoids in ruminant diets until recently, analyses of forages are often incomplete, focusing mainly on β-carotene and lutein. Carotenoid composition of green forage from middle mountain meadow was analyzed by HPLC after extraction and elimination of chlorophylls by mild saponification. This method of analysis uses two C
18
columns in series with a quaternary gradient system. Our method allowed, for the first time, the identification and quantification of several xanthophylls other than lutein (i.e., violaxanthin, antheraxanthin, epilutein) in chlorophyll-free extracts from carotenoid-rich forage. The intra-day (3.5–7.5 %) and inter-day (1.2–3.5 %) coefficients of variation are suitable for routine determination of carotenoids in green forage. This method could also be used in metabolic studies of these micronutrients in ruminants. Key words: Xanthophylls, carotenoids, fresh forage, HPLC |
| doi_str_mv | 10.4141/CJPS07133 |
| format | Article |
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18
columns in series with a quaternary gradient system. Our method allowed, for the first time, the identification and quantification of several xanthophylls other than lutein (i.e., violaxanthin, antheraxanthin, epilutein) in chlorophyll-free extracts from carotenoid-rich forage. The intra-day (3.5–7.5 %) and inter-day (1.2–3.5 %) coefficients of variation are suitable for routine determination of carotenoids in green forage. This method could also be used in metabolic studies of these micronutrients in ruminants. Key words: Xanthophylls, carotenoids, fresh forage, HPLC</description><identifier>ISSN: 0008-4220</identifier><identifier>EISSN: 1918-1833</identifier><identifier>DOI: 10.4141/CJPS07133</identifier><identifier>CODEN: CPLSAY</identifier><language>eng</language><publisher>Ottawa, ON: Agricultural Institute of Canada</publisher><subject>Agronomy. Soil science and plant productions ; Analytical chemistry ; Biological and medical sciences ; carotenoids ; chemical composition ; Chemical Sciences ; feed composition ; forage composition ; Fundamental and applied biological sciences. Psychology ; green forage ; high performance liquid chromatography ; Life Sciences ; methodology ; Vegetal Biology ; xanthophylls</subject><ispartof>Canadian journal of plant science, 2008, Vol.88 (6), p.1057-1064</ispartof><rights>2009 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-b9a30aea36ad90edcf1f4febf7a578b85af4f630a49664a963ccec48def32753</citedby><orcidid>0009-0008-8777-5533</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,881,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21019374$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02659654$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Cardinault, N</creatorcontrib><creatorcontrib>Lyan, B</creatorcontrib><creatorcontrib>Doreau, M</creatorcontrib><creatorcontrib>Chauveau, B</creatorcontrib><creatorcontrib>Rock, E</creatorcontrib><creatorcontrib>Grolier, P</creatorcontrib><title>Development of a method to determine carotenoid composition of fresh forages</title><title>Canadian journal of plant science</title><description>Due to the limited interest in carotenoids in ruminant diets until recently, analyses of forages are often incomplete, focusing mainly on β-carotene and lutein. Carotenoid composition of green forage from middle mountain meadow was analyzed by HPLC after extraction and elimination of chlorophylls by mild saponification. This method of analysis uses two C
18
columns in series with a quaternary gradient system. Our method allowed, for the first time, the identification and quantification of several xanthophylls other than lutein (i.e., violaxanthin, antheraxanthin, epilutein) in chlorophyll-free extracts from carotenoid-rich forage. The intra-day (3.5–7.5 %) and inter-day (1.2–3.5 %) coefficients of variation are suitable for routine determination of carotenoids in green forage. This method could also be used in metabolic studies of these micronutrients in ruminants. Key words: Xanthophylls, carotenoids, fresh forage, HPLC</description><subject>Agronomy. Soil science and plant productions</subject><subject>Analytical chemistry</subject><subject>Biological and medical sciences</subject><subject>carotenoids</subject><subject>chemical composition</subject><subject>Chemical Sciences</subject><subject>feed composition</subject><subject>forage composition</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>green forage</subject><subject>high performance liquid chromatography</subject><subject>Life Sciences</subject><subject>methodology</subject><subject>Vegetal Biology</subject><subject>xanthophylls</subject><issn>0008-4220</issn><issn>1918-1833</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNpF0MFKw0AQBuBFFKzVg0_gXjx4iM5mN8nmWGq1SkCh9Rymm9k2kmTDbij49qZU6mn4h--fwzB2K-BRCSWe5u-fK8iElGdsInKhI6GlPGcTANCRimO4ZFchfI8xExomrHimPTWub6kbuLMceUvDzlV8cLyigXxbd8QNejdQ5-qKG9f2LtRD7bqDt57CjlvncUvhml1YbALd_M0pW78s1vNlVHy8vs1nRWRkEg_RJkcJSChTrHKgylhhlaWNzTDJ9EYnOMZ0JCpPU4V5Ko0ho3RFVsZZIqfs4Xh2h03Z-7pF_1M6rMvlrCgPO4jTJE8TtRf_1ngXgid7KggoDx8rTx8b7f3R9hgMNtZjZ-pwKsQCRC4zNbq7o7PoStz60XytYhASRKIh0yB_Afc5dIs</recordid><startdate>2008</startdate><enddate>2008</enddate><creator>Cardinault, N</creator><creator>Lyan, B</creator><creator>Doreau, M</creator><creator>Chauveau, B</creator><creator>Rock, E</creator><creator>Grolier, P</creator><general>Agricultural Institute of Canada</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>1XC</scope><orcidid>https://orcid.org/0009-0008-8777-5533</orcidid></search><sort><creationdate>2008</creationdate><title>Development of a method to determine carotenoid composition of fresh forages</title><author>Cardinault, N ; Lyan, B ; Doreau, M ; Chauveau, B ; Rock, E ; Grolier, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-b9a30aea36ad90edcf1f4febf7a578b85af4f630a49664a963ccec48def32753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Analytical chemistry</topic><topic>Biological and medical sciences</topic><topic>carotenoids</topic><topic>chemical composition</topic><topic>Chemical Sciences</topic><topic>feed composition</topic><topic>forage composition</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>green forage</topic><topic>high performance liquid chromatography</topic><topic>Life Sciences</topic><topic>methodology</topic><topic>Vegetal Biology</topic><topic>xanthophylls</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cardinault, N</creatorcontrib><creatorcontrib>Lyan, B</creatorcontrib><creatorcontrib>Doreau, M</creatorcontrib><creatorcontrib>Chauveau, B</creatorcontrib><creatorcontrib>Rock, E</creatorcontrib><creatorcontrib>Grolier, P</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Canadian journal of plant science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cardinault, N</au><au>Lyan, B</au><au>Doreau, M</au><au>Chauveau, B</au><au>Rock, E</au><au>Grolier, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a method to determine carotenoid composition of fresh forages</atitle><jtitle>Canadian journal of plant science</jtitle><date>2008</date><risdate>2008</risdate><volume>88</volume><issue>6</issue><spage>1057</spage><epage>1064</epage><pages>1057-1064</pages><issn>0008-4220</issn><eissn>1918-1833</eissn><coden>CPLSAY</coden><abstract>Due to the limited interest in carotenoids in ruminant diets until recently, analyses of forages are often incomplete, focusing mainly on β-carotene and lutein. Carotenoid composition of green forage from middle mountain meadow was analyzed by HPLC after extraction and elimination of chlorophylls by mild saponification. This method of analysis uses two C
18
columns in series with a quaternary gradient system. Our method allowed, for the first time, the identification and quantification of several xanthophylls other than lutein (i.e., violaxanthin, antheraxanthin, epilutein) in chlorophyll-free extracts from carotenoid-rich forage. The intra-day (3.5–7.5 %) and inter-day (1.2–3.5 %) coefficients of variation are suitable for routine determination of carotenoids in green forage. This method could also be used in metabolic studies of these micronutrients in ruminants. Key words: Xanthophylls, carotenoids, fresh forage, HPLC</abstract><cop>Ottawa, ON</cop><pub>Agricultural Institute of Canada</pub><doi>10.4141/CJPS07133</doi><tpages>8</tpages><orcidid>https://orcid.org/0009-0008-8777-5533</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Canadian journal of plant science, 2008, Vol.88 (6), p.1057-1064 |
| issn | 0008-4220 1918-1833 |
| language | eng |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Agronomy. Soil science and plant productions Analytical chemistry Biological and medical sciences carotenoids chemical composition Chemical Sciences feed composition forage composition Fundamental and applied biological sciences. Psychology green forage high performance liquid chromatography Life Sciences methodology Vegetal Biology xanthophylls |
| title | Development of a method to determine carotenoid composition of fresh forages |
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