Leucoanthocyanidin reductase and anthocyanidin reductase gene expression and activity in flowers, young berries and skins of Vitis vinifera L. cv. Cabernet-Sauvignon during development
Proanthocyanidins, or condensed tannins, are crucial polyphenolic compounds for grape and wine quality. Recently, significant advances were achieved in understanding the biosynthesis of their main subunits: (+)-catechin and (−)-epicatechin, produced by catalysis of leucoanthocyanidin reductase (LAR)...
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| Veröffentlicht in: | Plant physiology and biochemistry 2009-04, Vol.47 (4), p.282-290 |
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| creator | Gagné, Séverine Lacampagne, Soizic Claisse, Olivier Gény, Laurence |
| description | Proanthocyanidins, or condensed tannins, are crucial polyphenolic compounds for grape and wine quality. Recently, significant advances were achieved in understanding the biosynthesis of their main subunits: (+)-catechin and (−)-epicatechin, produced by catalysis of leucoanthocyanidin reductase (LAR) and anthocyanidin reductase (ANR), respectively. Expression studies had been published but no data were available on enzyme activity. In our work, we devised assays to measure LAR and ANR activity and determine their development throughout the growth of flowers, young berries, and skins of
Vitis vinifera L. cv. Cabernet-Sauvignon. We also investigated the accumulation of compounds in these tissues and focused on the expression of both the structural genes and the transcription factors involved in regulating them:
VvMYB5a and
VvMYBPA1. Biosynthetic genes were expressed early and LAR and ANR were already active during flowering and at the beginning of berry growth, as well as during colour-change in skins. The profiles we determined correlated with total tannin, catechin, and epicatechin concentrations. The involvement of
VvMYB5a and
VvMYBPA1 was confirmed and specific expression patterns were also established for
VvLAR transcripts. |
| doi_str_mv | 10.1016/j.plaphy.2008.12.004 |
| format | Article |
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Vitis vinifera L. cv. Cabernet-Sauvignon. We also investigated the accumulation of compounds in these tissues and focused on the expression of both the structural genes and the transcription factors involved in regulating them:
VvMYB5a and
VvMYBPA1. Biosynthetic genes were expressed early and LAR and ANR were already active during flowering and at the beginning of berry growth, as well as during colour-change in skins. The profiles we determined correlated with total tannin, catechin, and epicatechin concentrations. The involvement of
VvMYB5a and
VvMYBPA1 was confirmed and specific expression patterns were also established for
VvLAR transcripts.</description><identifier>ISSN: 0981-9428</identifier><identifier>EISSN: 1873-2690</identifier><identifier>DOI: 10.1016/j.plaphy.2008.12.004</identifier><identifier>PMID: 19136268</identifier><identifier>CODEN: PPBIEX</identifier><language>eng</language><publisher>Paris: Elsevier Masson SAS</publisher><subject>Anthocyanidin reductase ; Anthocyanins - metabolism ; Biochemistry, Molecular Biology ; Biological and medical sciences ; Flowers ; Flowers - enzymology ; Flowers - growth & development ; Flowers - metabolism ; Fruit - enzymology ; Fruit - growth & development ; Fruit - metabolism ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Plant ; Leucoanthocyanidin reductase ; Life Sciences ; NADH, NADPH Oxidoreductases - genetics ; NADH, NADPH Oxidoreductases - metabolism ; Plant physiology and development ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Proanthocyanidin ; Skin ; Vitaceae ; Vitis - enzymology ; Vitis - growth & development ; Vitis - metabolism ; Vitis vinifera L</subject><ispartof>Plant physiology and biochemistry, 2009-04, Vol.47 (4), p.282-290</ispartof><rights>2008 Elsevier Masson SAS</rights><rights>2009 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c521t-d8de567dd41293d5f42d620a985803a1316dee0a9a002764df0ca86a9df0ce13</citedby><cites>FETCH-LOGICAL-c521t-d8de567dd41293d5f42d620a985803a1316dee0a9a002764df0ca86a9df0ce13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.plaphy.2008.12.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21326846$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19136268$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02659261$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Gagné, Séverine</creatorcontrib><creatorcontrib>Lacampagne, Soizic</creatorcontrib><creatorcontrib>Claisse, Olivier</creatorcontrib><creatorcontrib>Gény, Laurence</creatorcontrib><title>Leucoanthocyanidin reductase and anthocyanidin reductase gene expression and activity in flowers, young berries and skins of Vitis vinifera L. cv. Cabernet-Sauvignon during development</title><title>Plant physiology and biochemistry</title><addtitle>Plant Physiol Biochem</addtitle><description>Proanthocyanidins, or condensed tannins, are crucial polyphenolic compounds for grape and wine quality. Recently, significant advances were achieved in understanding the biosynthesis of their main subunits: (+)-catechin and (−)-epicatechin, produced by catalysis of leucoanthocyanidin reductase (LAR) and anthocyanidin reductase (ANR), respectively. Expression studies had been published but no data were available on enzyme activity. In our work, we devised assays to measure LAR and ANR activity and determine their development throughout the growth of flowers, young berries, and skins of
Vitis vinifera L. cv. Cabernet-Sauvignon. We also investigated the accumulation of compounds in these tissues and focused on the expression of both the structural genes and the transcription factors involved in regulating them:
VvMYB5a and
VvMYBPA1. Biosynthetic genes were expressed early and LAR and ANR were already active during flowering and at the beginning of berry growth, as well as during colour-change in skins. The profiles we determined correlated with total tannin, catechin, and epicatechin concentrations. The involvement of
VvMYB5a and
VvMYBPA1 was confirmed and specific expression patterns were also established for
VvLAR transcripts.</description><subject>Anthocyanidin reductase</subject><subject>Anthocyanins - metabolism</subject><subject>Biochemistry, Molecular Biology</subject><subject>Biological and medical sciences</subject><subject>Flowers</subject><subject>Flowers - enzymology</subject><subject>Flowers - growth & development</subject><subject>Flowers - metabolism</subject><subject>Fruit - enzymology</subject><subject>Fruit - growth & development</subject><subject>Fruit - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Plant</subject><subject>Leucoanthocyanidin reductase</subject><subject>Life Sciences</subject><subject>NADH, NADPH Oxidoreductases - genetics</subject><subject>NADH, NADPH Oxidoreductases - metabolism</subject><subject>Plant physiology and development</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Proanthocyanidin</subject><subject>Skin</subject><subject>Vitaceae</subject><subject>Vitis - enzymology</subject><subject>Vitis - growth & development</subject><subject>Vitis - metabolism</subject><subject>Vitis vinifera L</subject><issn>0981-9428</issn><issn>1873-2690</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0l-L1DAQAPAiireefgORvCgItiZpm7YvB8einrDgg4evYS6Z7mbtJjVpq_1mfjxTu5xP6lP-_TIMM5MkzxnNGGXi7THrO-gPc8YprTPGM0qLB8mG1VWectHQh8mGNjVLm4LXF8mTEI6UUl5U-ePkgjUsF1zUm-TnDkflwA4Hp2awRhtLPOpRDRCQgNXkb297tEjwR-8xBOPsatVgJjPMJMq2c9_RhzdkdqPdkzv03mD4zcJXYwNxLfliBhPIZKxp0QPZZURNGdlCxBaH9DOMk9nbGFyP3sQgGifsXH9COzxNHrXQBXx2Xi-T2_fvbrc36e7Th4_b612qSs6GVNcaS1FpXTDe5LpsC64Fp9DUZU1zYDkTGjGeIRanEoVuqYJaQLNskOWXyes17AE62XtzAj9LB0beXO_kcke5KBsu2LTYV6vtvfs2YhjkyQSFXQcW3RikqGKnGK3-CzllvGpEHmGxQuVdCB7b-xQYlcsUyKNcp0AuUyAZl3EK4rcX5_jj3Qn1n0_ntkfw8gwgKOhaD1aZcO84y6MqRHRXq8NY4cmgl0EZtAq18agGqZ35dya_ANRZ1fs</recordid><startdate>20090401</startdate><enddate>20090401</enddate><creator>Gagné, Séverine</creator><creator>Lacampagne, Soizic</creator><creator>Claisse, Olivier</creator><creator>Gény, Laurence</creator><general>Elsevier Masson SAS</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>1XC</scope></search><sort><creationdate>20090401</creationdate><title>Leucoanthocyanidin reductase and anthocyanidin reductase gene expression and activity in flowers, young berries and skins of Vitis vinifera L. cv. Cabernet-Sauvignon during development</title><author>Gagné, Séverine ; Lacampagne, Soizic ; Claisse, Olivier ; Gény, Laurence</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c521t-d8de567dd41293d5f42d620a985803a1316dee0a9a002764df0ca86a9df0ce13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Anthocyanidin reductase</topic><topic>Anthocyanins - metabolism</topic><topic>Biochemistry, Molecular Biology</topic><topic>Biological and medical sciences</topic><topic>Flowers</topic><topic>Flowers - enzymology</topic><topic>Flowers - growth & development</topic><topic>Flowers - metabolism</topic><topic>Fruit - enzymology</topic><topic>Fruit - growth & development</topic><topic>Fruit - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Plant</topic><topic>Leucoanthocyanidin reductase</topic><topic>Life Sciences</topic><topic>NADH, NADPH Oxidoreductases - genetics</topic><topic>NADH, NADPH Oxidoreductases - metabolism</topic><topic>Plant physiology and development</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Proanthocyanidin</topic><topic>Skin</topic><topic>Vitaceae</topic><topic>Vitis - enzymology</topic><topic>Vitis - growth & development</topic><topic>Vitis - metabolism</topic><topic>Vitis vinifera L</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gagné, Séverine</creatorcontrib><creatorcontrib>Lacampagne, Soizic</creatorcontrib><creatorcontrib>Claisse, Olivier</creatorcontrib><creatorcontrib>Gény, Laurence</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Plant physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gagné, Séverine</au><au>Lacampagne, Soizic</au><au>Claisse, Olivier</au><au>Gény, Laurence</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Leucoanthocyanidin reductase and anthocyanidin reductase gene expression and activity in flowers, young berries and skins of Vitis vinifera L. cv. Cabernet-Sauvignon during development</atitle><jtitle>Plant physiology and biochemistry</jtitle><addtitle>Plant Physiol Biochem</addtitle><date>2009-04-01</date><risdate>2009</risdate><volume>47</volume><issue>4</issue><spage>282</spage><epage>290</epage><pages>282-290</pages><issn>0981-9428</issn><eissn>1873-2690</eissn><coden>PPBIEX</coden><abstract>Proanthocyanidins, or condensed tannins, are crucial polyphenolic compounds for grape and wine quality. Recently, significant advances were achieved in understanding the biosynthesis of their main subunits: (+)-catechin and (−)-epicatechin, produced by catalysis of leucoanthocyanidin reductase (LAR) and anthocyanidin reductase (ANR), respectively. Expression studies had been published but no data were available on enzyme activity. In our work, we devised assays to measure LAR and ANR activity and determine their development throughout the growth of flowers, young berries, and skins of
Vitis vinifera L. cv. Cabernet-Sauvignon. We also investigated the accumulation of compounds in these tissues and focused on the expression of both the structural genes and the transcription factors involved in regulating them:
VvMYB5a and
VvMYBPA1. Biosynthetic genes were expressed early and LAR and ANR were already active during flowering and at the beginning of berry growth, as well as during colour-change in skins. The profiles we determined correlated with total tannin, catechin, and epicatechin concentrations. The involvement of
VvMYB5a and
VvMYBPA1 was confirmed and specific expression patterns were also established for
VvLAR transcripts.</abstract><cop>Paris</cop><pub>Elsevier Masson SAS</pub><pmid>19136268</pmid><doi>10.1016/j.plaphy.2008.12.004</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0981-9428 |
| ispartof | Plant physiology and biochemistry, 2009-04, Vol.47 (4), p.282-290 |
| issn | 0981-9428 1873-2690 |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_02659261v1 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Anthocyanidin reductase Anthocyanins - metabolism Biochemistry, Molecular Biology Biological and medical sciences Flowers Flowers - enzymology Flowers - growth & development Flowers - metabolism Fruit - enzymology Fruit - growth & development Fruit - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant Leucoanthocyanidin reductase Life Sciences NADH, NADPH Oxidoreductases - genetics NADH, NADPH Oxidoreductases - metabolism Plant physiology and development Plant Proteins - genetics Plant Proteins - metabolism Proanthocyanidin Skin Vitaceae Vitis - enzymology Vitis - growth & development Vitis - metabolism Vitis vinifera L |
| title | Leucoanthocyanidin reductase and anthocyanidin reductase gene expression and activity in flowers, young berries and skins of Vitis vinifera L. cv. Cabernet-Sauvignon during development |
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