Leptin and its receptors are present in the rat olfactory mucosa and modulated by the nutritional status
Abstract Leptin is an adipocyte-derived cytokine that regulates body weight mainly via the long form of the leptin receptor (Ob-Rb). Leptin and its receptors are expressed in several tissues, suggesting that leptin might also be effective peripherally. We hypothesized that, as shown in taste cells,...
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| Veröffentlicht in: | Brain research 2007-01, Vol.1129 (1), p.130-141 |
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| creator | Baly, Christine Aioun, Josiane Badonnel, Karine Lacroix, Marie-Christine Durieux, Didier Schlegel, Claire Salesse, Roland Caillol, Monique |
| description | Abstract Leptin is an adipocyte-derived cytokine that regulates body weight mainly via the long form of the leptin receptor (Ob-Rb). Leptin and its receptors are expressed in several tissues, suggesting that leptin might also be effective peripherally. We hypothesized that, as shown in taste cells, leptin and its receptors isoforms (Ob-Rs) could be present in the rat olfactory mucosa (OM). Using RT-PCR, light and electron microscopy immunohistochemistry (ICC), we found that different isoforms of the receptor were expressed in OM and localized in sustentacular cells and in a subpopulation of maturating neurons; in addition, immunoreactivity was also present in differentiated neurons and enriched at the cilia membranes, where the odorants bind to their receptors. Moreover, using RT-PCR, ICC and RIA measurements, we showed that leptin is synthesized locally in the olfactory mucosa. In addition, we demonstrate that fasting causes a significant enhanced transcription of both leptin and Ob-Rs in rat OM by quantitative RT-PCR data. Altogether, these results strongly suggested that leptin, acting as an endocrine or a paracrine factor, could be an important regulator of olfactory function, as a neuromodulator of the olfactory message in cilia of mature olfactory receptors neurons (ORN), but also for the homeostasis of this complex tissue, acting on differentiating neurons and on sustentacular cells. |
| doi_str_mv | 10.1016/j.brainres.2006.10.030 |
| format | Article |
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Leptin and its receptors are expressed in several tissues, suggesting that leptin might also be effective peripherally. We hypothesized that, as shown in taste cells, leptin and its receptors isoforms (Ob-Rs) could be present in the rat olfactory mucosa (OM). Using RT-PCR, light and electron microscopy immunohistochemistry (ICC), we found that different isoforms of the receptor were expressed in OM and localized in sustentacular cells and in a subpopulation of maturating neurons; in addition, immunoreactivity was also present in differentiated neurons and enriched at the cilia membranes, where the odorants bind to their receptors. Moreover, using RT-PCR, ICC and RIA measurements, we showed that leptin is synthesized locally in the olfactory mucosa. In addition, we demonstrate that fasting causes a significant enhanced transcription of both leptin and Ob-Rs in rat OM by quantitative RT-PCR data. Altogether, these results strongly suggested that leptin, acting as an endocrine or a paracrine factor, could be an important regulator of olfactory function, as a neuromodulator of the olfactory message in cilia of mature olfactory receptors neurons (ORN), but also for the homeostasis of this complex tissue, acting on differentiating neurons and on sustentacular cells.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>EISSN: 0006-8993</identifier><identifier>DOI: 10.1016/j.brainres.2006.10.030</identifier><identifier>PMID: 17169337</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Animals ; Appetite Regulation - physiology ; Biological and medical sciences ; Cilia - metabolism ; Cilia - ultrastructure ; Computer Science ; Electron microscopy ; Food Deprivation - physiology ; Food intake ; Fundamental and applied biological sciences. Psychology ; Hunger - physiology ; Immunohistochemistry ; Leptin ; Leptin - genetics ; Leptin - metabolism ; Leptin receptors ; Life Sciences ; Male ; Microscopy, Electron, Transmission ; Neurology ; Neurosecretory Systems - metabolism ; Nutritional Status - physiology ; Olfactory mucosa ; Olfactory Mucosa - metabolism ; Olfactory Mucosa - ultrastructure ; Olfactory Receptor Neurons - metabolism ; Olfactory Receptor Neurons - ultrastructure ; Olfactory system and olfaction. Gustatory system and gustation ; Rats ; Receptors, Cell Surface - genetics ; Receptors, Cell Surface - metabolism ; Receptors, Leptin ; RNA, Messenger - metabolism ; Smell - physiology ; Transcriptional Activation - physiology ; Up-Regulation - physiology ; Vertebrates: nervous system and sense organs</subject><ispartof>Brain research, 2007-01, Vol.1129 (1), p.130-141</ispartof><rights>Elsevier B.V.</rights><rights>2006 Elsevier B.V.</rights><rights>2007 INIST-CNRS</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c582t-f2a13ef63097f9e08896d13490f23466d0b4aeab1fc89e8ad4941be503812b7a3</citedby><cites>FETCH-LOGICAL-c582t-f2a13ef63097f9e08896d13490f23466d0b4aeab1fc89e8ad4941be503812b7a3</cites><orcidid>0000-0003-3755-1538 ; 0000-0002-8966-9522</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.brainres.2006.10.030$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18440482$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17169337$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02658203$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Baly, Christine</creatorcontrib><creatorcontrib>Aioun, Josiane</creatorcontrib><creatorcontrib>Badonnel, Karine</creatorcontrib><creatorcontrib>Lacroix, Marie-Christine</creatorcontrib><creatorcontrib>Durieux, Didier</creatorcontrib><creatorcontrib>Schlegel, Claire</creatorcontrib><creatorcontrib>Salesse, Roland</creatorcontrib><creatorcontrib>Caillol, Monique</creatorcontrib><title>Leptin and its receptors are present in the rat olfactory mucosa and modulated by the nutritional status</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>Abstract Leptin is an adipocyte-derived cytokine that regulates body weight mainly via the long form of the leptin receptor (Ob-Rb). Leptin and its receptors are expressed in several tissues, suggesting that leptin might also be effective peripherally. We hypothesized that, as shown in taste cells, leptin and its receptors isoforms (Ob-Rs) could be present in the rat olfactory mucosa (OM). Using RT-PCR, light and electron microscopy immunohistochemistry (ICC), we found that different isoforms of the receptor were expressed in OM and localized in sustentacular cells and in a subpopulation of maturating neurons; in addition, immunoreactivity was also present in differentiated neurons and enriched at the cilia membranes, where the odorants bind to their receptors. Moreover, using RT-PCR, ICC and RIA measurements, we showed that leptin is synthesized locally in the olfactory mucosa. In addition, we demonstrate that fasting causes a significant enhanced transcription of both leptin and Ob-Rs in rat OM by quantitative RT-PCR data. Altogether, these results strongly suggested that leptin, acting as an endocrine or a paracrine factor, could be an important regulator of olfactory function, as a neuromodulator of the olfactory message in cilia of mature olfactory receptors neurons (ORN), but also for the homeostasis of this complex tissue, acting on differentiating neurons and on sustentacular cells.</description><subject>Animals</subject><subject>Appetite Regulation - physiology</subject><subject>Biological and medical sciences</subject><subject>Cilia - metabolism</subject><subject>Cilia - ultrastructure</subject><subject>Computer Science</subject><subject>Electron microscopy</subject><subject>Food Deprivation - physiology</subject><subject>Food intake</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hunger - physiology</subject><subject>Immunohistochemistry</subject><subject>Leptin</subject><subject>Leptin - genetics</subject><subject>Leptin - metabolism</subject><subject>Leptin receptors</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Microscopy, Electron, Transmission</subject><subject>Neurology</subject><subject>Neurosecretory Systems - metabolism</subject><subject>Nutritional Status - physiology</subject><subject>Olfactory mucosa</subject><subject>Olfactory Mucosa - metabolism</subject><subject>Olfactory Mucosa - ultrastructure</subject><subject>Olfactory Receptor Neurons - metabolism</subject><subject>Olfactory Receptor Neurons - ultrastructure</subject><subject>Olfactory system and olfaction. Gustatory system and gustation</subject><subject>Rats</subject><subject>Receptors, Cell Surface - genetics</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Receptors, Leptin</subject><subject>RNA, Messenger - metabolism</subject><subject>Smell - physiology</subject><subject>Transcriptional Activation - physiology</subject><subject>Up-Regulation - physiology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0006-8993</issn><issn>1872-6240</issn><issn>0006-8993</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk9vEzEQxVcIREPhK1S-gMQhYfwnXvuCqCqgSJE4AGdr1jurOGx2g-2tlG9fbxOoxKUny8-_NzPym6q64rDiwPWH3aqJGIZIaSUAdBFXIOFZteCmFkstFDyvFlBelsZaeVG9SmlXrlJaeFld8JprK2W9qLYbOuQwMBxaFnJikXwRxpgYRmKHUp-GzAqQt8QiZjb2HfoCHNl-8mPCB-d-bKceM7WsOT6Qw5RjyGEcsGcpY57S6-pFh32iN-fzsvr15fPPm9vl5vvXbzfXm6VfG5GXnUAuqdMSbN1ZAmOsbrlUFjohldYtNAoJG955Y8lgq6ziDa1BGi6aGuVl9f5Ud4u9O8Swx3h0IwZ3e71xswZCl04g73hh353YQxz_TJSy24fkqe9xoHFKThslQKv1k6AAYaS1uoD6BPo4phSp-zcCBzcH53bub3BuDm7WS3DFeHXuMDV7ah9t56QK8PYMYPLYdxEHH9IjZ5QCZUThPp04Kp98Fyi65AMNntpQss2uHcPTs3z8r4TvwxBK1990pLQbp1hyTY67JBy4H_OazVsGurhrKeU9ebHOqw</recordid><startdate>20070119</startdate><enddate>20070119</enddate><creator>Baly, Christine</creator><creator>Aioun, Josiane</creator><creator>Badonnel, Karine</creator><creator>Lacroix, Marie-Christine</creator><creator>Durieux, Didier</creator><creator>Schlegel, Claire</creator><creator>Salesse, Roland</creator><creator>Caillol, Monique</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0003-3755-1538</orcidid><orcidid>https://orcid.org/0000-0002-8966-9522</orcidid></search><sort><creationdate>20070119</creationdate><title>Leptin and its receptors are present in the rat olfactory mucosa and modulated by the nutritional status</title><author>Baly, Christine ; Aioun, Josiane ; Badonnel, Karine ; Lacroix, Marie-Christine ; Durieux, Didier ; Schlegel, Claire ; Salesse, Roland ; Caillol, Monique</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c582t-f2a13ef63097f9e08896d13490f23466d0b4aeab1fc89e8ad4941be503812b7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Appetite Regulation - physiology</topic><topic>Biological and medical sciences</topic><topic>Cilia - metabolism</topic><topic>Cilia - ultrastructure</topic><topic>Computer Science</topic><topic>Electron microscopy</topic><topic>Food Deprivation - physiology</topic><topic>Food intake</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hunger - physiology</topic><topic>Immunohistochemistry</topic><topic>Leptin</topic><topic>Leptin - genetics</topic><topic>Leptin - metabolism</topic><topic>Leptin receptors</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Microscopy, Electron, Transmission</topic><topic>Neurology</topic><topic>Neurosecretory Systems - metabolism</topic><topic>Nutritional Status - physiology</topic><topic>Olfactory mucosa</topic><topic>Olfactory Mucosa - metabolism</topic><topic>Olfactory Mucosa - ultrastructure</topic><topic>Olfactory Receptor Neurons - metabolism</topic><topic>Olfactory Receptor Neurons - ultrastructure</topic><topic>Olfactory system and olfaction. Gustatory system and gustation</topic><topic>Rats</topic><topic>Receptors, Cell Surface - genetics</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>Receptors, Leptin</topic><topic>RNA, Messenger - metabolism</topic><topic>Smell - physiology</topic><topic>Transcriptional Activation - physiology</topic><topic>Up-Regulation - physiology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baly, Christine</creatorcontrib><creatorcontrib>Aioun, Josiane</creatorcontrib><creatorcontrib>Badonnel, Karine</creatorcontrib><creatorcontrib>Lacroix, Marie-Christine</creatorcontrib><creatorcontrib>Durieux, Didier</creatorcontrib><creatorcontrib>Schlegel, Claire</creatorcontrib><creatorcontrib>Salesse, Roland</creatorcontrib><creatorcontrib>Caillol, Monique</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baly, Christine</au><au>Aioun, Josiane</au><au>Badonnel, Karine</au><au>Lacroix, Marie-Christine</au><au>Durieux, Didier</au><au>Schlegel, Claire</au><au>Salesse, Roland</au><au>Caillol, Monique</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Leptin and its receptors are present in the rat olfactory mucosa and modulated by the nutritional status</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>2007-01-19</date><risdate>2007</risdate><volume>1129</volume><issue>1</issue><spage>130</spage><epage>141</epage><pages>130-141</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><eissn>0006-8993</eissn><coden>BRREAP</coden><abstract>Abstract Leptin is an adipocyte-derived cytokine that regulates body weight mainly via the long form of the leptin receptor (Ob-Rb). Leptin and its receptors are expressed in several tissues, suggesting that leptin might also be effective peripherally. We hypothesized that, as shown in taste cells, leptin and its receptors isoforms (Ob-Rs) could be present in the rat olfactory mucosa (OM). Using RT-PCR, light and electron microscopy immunohistochemistry (ICC), we found that different isoforms of the receptor were expressed in OM and localized in sustentacular cells and in a subpopulation of maturating neurons; in addition, immunoreactivity was also present in differentiated neurons and enriched at the cilia membranes, where the odorants bind to their receptors. Moreover, using RT-PCR, ICC and RIA measurements, we showed that leptin is synthesized locally in the olfactory mucosa. In addition, we demonstrate that fasting causes a significant enhanced transcription of both leptin and Ob-Rs in rat OM by quantitative RT-PCR data. Altogether, these results strongly suggested that leptin, acting as an endocrine or a paracrine factor, could be an important regulator of olfactory function, as a neuromodulator of the olfactory message in cilia of mature olfactory receptors neurons (ORN), but also for the homeostasis of this complex tissue, acting on differentiating neurons and on sustentacular cells.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>17169337</pmid><doi>10.1016/j.brainres.2006.10.030</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-3755-1538</orcidid><orcidid>https://orcid.org/0000-0002-8966-9522</orcidid></addata></record> |
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| subjects | Animals Appetite Regulation - physiology Biological and medical sciences Cilia - metabolism Cilia - ultrastructure Computer Science Electron microscopy Food Deprivation - physiology Food intake Fundamental and applied biological sciences. Psychology Hunger - physiology Immunohistochemistry Leptin Leptin - genetics Leptin - metabolism Leptin receptors Life Sciences Male Microscopy, Electron, Transmission Neurology Neurosecretory Systems - metabolism Nutritional Status - physiology Olfactory mucosa Olfactory Mucosa - metabolism Olfactory Mucosa - ultrastructure Olfactory Receptor Neurons - metabolism Olfactory Receptor Neurons - ultrastructure Olfactory system and olfaction. Gustatory system and gustation Rats Receptors, Cell Surface - genetics Receptors, Cell Surface - metabolism Receptors, Leptin RNA, Messenger - metabolism Smell - physiology Transcriptional Activation - physiology Up-Regulation - physiology Vertebrates: nervous system and sense organs |
| title | Leptin and its receptors are present in the rat olfactory mucosa and modulated by the nutritional status |
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