Apoptotic pulsed dendritic cells induce a protective immune response against Toxoplasma gondii
Infection with the intracellular protozoan parasite Toxoplasma gondii may cause severe sequelae in foetuses and life-threatening neuropathy in immunocompromised patients. We recently reported that vaccination with T. gondii-pulsed dendritic cells induces protective humoral and cellular immune respon...
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| Veröffentlicht in: | Parasite immunology 2008-11, Vol.30 (11-12), p.620-629 |
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| container_title | Parasite immunology |
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| creator | BERTAUX, L MEVELEC, M.-N DION, S SURAUD, V GREGOIRE, M BERTHON, P DIMIER-POISSON, I |
| description | Infection with the intracellular protozoan parasite Toxoplasma gondii may cause severe sequelae in foetuses and life-threatening neuropathy in immunocompromised patients. We recently reported that vaccination with T. gondii-pulsed dendritic cells induces protective humoral and cellular immune responses against this intracellular pathogen in CBA/J mice. We assessed the feasibility of using a nonlive vaccine, by inducing the apoptosis of T. gondii-pulsed dendritic cells before injecting them into mice. Apoptosis was induced by culturing cells to confluence. We investigated whether these apoptotic T. gondii-pulsed dendritic cells elicited an immune response in vivo. Some studies have shown that immunization with apoptotic cells leads to the activation of innate and adaptive immune mechanisms. Our results are consistent with apoptotic cells having immunomodulatory properties in a model of parasite infection. We showed that the adoptive transfer of T. gondii-pulsed apoptotic dendritic cells elicited humoral and cellular Toxoplasma-specific immune responses with a Th1/Th2 profile, and conferred specific protection. The protective immune response induced was independent of inducible HSP70 production by apoptotic dendritic cells. |
| doi_str_mv | 10.1111/j.1365-3024.2008.01064.x |
| format | Article |
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We recently reported that vaccination with T. gondii-pulsed dendritic cells induces protective humoral and cellular immune responses against this intracellular pathogen in CBA/J mice. We assessed the feasibility of using a nonlive vaccine, by inducing the apoptosis of T. gondii-pulsed dendritic cells before injecting them into mice. Apoptosis was induced by culturing cells to confluence. We investigated whether these apoptotic T. gondii-pulsed dendritic cells elicited an immune response in vivo. Some studies have shown that immunization with apoptotic cells leads to the activation of innate and adaptive immune mechanisms. Our results are consistent with apoptotic cells having immunomodulatory properties in a model of parasite infection. We showed that the adoptive transfer of T. gondii-pulsed apoptotic dendritic cells elicited humoral and cellular Toxoplasma-specific immune responses with a Th1/Th2 profile, and conferred specific protection. The protective immune response induced was independent of inducible HSP70 production by apoptotic dendritic cells.</description><identifier>ISSN: 0141-9838</identifier><identifier>EISSN: 1365-3024</identifier><identifier>DOI: 10.1111/j.1365-3024.2008.01064.x</identifier><identifier>PMID: 19067843</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Adoptive Transfer ; Animals ; Antibodies, Protozoan - blood ; Apoptosis ; Cell Proliferation ; Cells, Cultured ; Cytokines - secretion ; dendritic cell ; Dendritic Cells - immunology ; Female ; immunization ; Leukocytes, Mononuclear - immunology ; Life Sciences ; Lymph Nodes - immunology ; Mice ; Mice, Inbred CBA ; Spleen - immunology ; Toxoplasma - immunology ; Toxoplasma gondii ; Toxoplasmosis - prevention & control ; Vaccination - methods</subject><ispartof>Parasite immunology, 2008-11, Vol.30 (11-12), p.620-629</ispartof><rights>2008 The Authors. 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We recently reported that vaccination with T. gondii-pulsed dendritic cells induces protective humoral and cellular immune responses against this intracellular pathogen in CBA/J mice. We assessed the feasibility of using a nonlive vaccine, by inducing the apoptosis of T. gondii-pulsed dendritic cells before injecting them into mice. Apoptosis was induced by culturing cells to confluence. We investigated whether these apoptotic T. gondii-pulsed dendritic cells elicited an immune response in vivo. Some studies have shown that immunization with apoptotic cells leads to the activation of innate and adaptive immune mechanisms. Our results are consistent with apoptotic cells having immunomodulatory properties in a model of parasite infection. We showed that the adoptive transfer of T. gondii-pulsed apoptotic dendritic cells elicited humoral and cellular Toxoplasma-specific immune responses with a Th1/Th2 profile, and conferred specific protection. The protective immune response induced was independent of inducible HSP70 production by apoptotic dendritic cells.</description><subject>Adoptive Transfer</subject><subject>Animals</subject><subject>Antibodies, Protozoan - blood</subject><subject>Apoptosis</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Cytokines - secretion</subject><subject>dendritic cell</subject><subject>Dendritic Cells - immunology</subject><subject>Female</subject><subject>immunization</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Life Sciences</subject><subject>Lymph Nodes - immunology</subject><subject>Mice</subject><subject>Mice, Inbred CBA</subject><subject>Spleen - immunology</subject><subject>Toxoplasma - immunology</subject><subject>Toxoplasma gondii</subject><subject>Toxoplasmosis - prevention & control</subject><subject>Vaccination - methods</subject><issn>0141-9838</issn><issn>1365-3024</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk1v1DAQhi0EokvhL1CfkDgk9WfWOXBYrVpaaSuQaK9YXnuy9SqJQ5yU7b-v06zKsfhia-Z5xzN-jRCmJKdpne9zyguZccJEzghROaGkEPnhDVq8JN6iBaGCZqXi6gR9iHFPCOWs4O_RCS1JsVSCL9DvVRe6IQze4m6sIzjsoHW9nwIW6jpi37rRAja468MAdvAPgH3TjC3gHmIX2piSO-PbOODbcAhdbWJj8C60zvuP6F1lUtlPx_0U3V1e3K6vss2P79fr1SazMjWeVcwptiVKFASENEUFpWSqEIYJCdZyS5dbwYUFKpyRYmsE55WikEajjqSZTtHXue69qXXX-8b0jzoYr69WGz3FEiSXpWAPNLFfZjYN9GeEOOjGx2lW00IYoy5KpVh6uVfB9PKSKyYSqGbQ9iHGHqqXFijRk2F6rydf9OTLJFP62TB9SNLPxzvGbQPun_DoUAK-zcBfX8PjfxfWP69vplPSn836ygRtdr2P-u4XSz-BUCklIYw_AXtFrLA</recordid><startdate>200811</startdate><enddate>200811</enddate><creator>BERTAUX, L</creator><creator>MEVELEC, M.-N</creator><creator>DION, S</creator><creator>SURAUD, V</creator><creator>GREGOIRE, M</creator><creator>BERTHON, P</creator><creator>DIMIER-POISSON, I</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Wiley</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>M7N</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-3491-7613</orcidid><orcidid>https://orcid.org/0000-0001-8917-4493</orcidid><orcidid>https://orcid.org/0000-0001-6784-8549</orcidid></search><sort><creationdate>200811</creationdate><title>Apoptotic pulsed dendritic cells induce a protective immune response against Toxoplasma gondii</title><author>BERTAUX, L ; 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| subjects | Adoptive Transfer Animals Antibodies, Protozoan - blood Apoptosis Cell Proliferation Cells, Cultured Cytokines - secretion dendritic cell Dendritic Cells - immunology Female immunization Leukocytes, Mononuclear - immunology Life Sciences Lymph Nodes - immunology Mice Mice, Inbred CBA Spleen - immunology Toxoplasma - immunology Toxoplasma gondii Toxoplasmosis - prevention & control Vaccination - methods |
| title | Apoptotic pulsed dendritic cells induce a protective immune response against Toxoplasma gondii |
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