Novel isolation of water-soluble polysaccharides from the fruiting bodies of Pleurotus ostreatus mushrooms
[Display omitted] ► Fruiting bodies of Pleurotus ostreatus were used for isolation of polysaccharides. ► Three fractions were obtained by cold water, hot water and hot NaOH extractions. ► PC, PH and PB were α-(1-3),(1-6)-galactan, α-(1-4)-glucan and β-(1-3),(1-6)-glucan. ► Their molecular masses wer...
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| Veröffentlicht in: | Carbohydrate research 2012-09, Vol.358, p.72-77 |
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| creator | Palacios, Irene García-Lafuente, Ana Guillamón, Eva Villares, Ana |
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► Fruiting bodies of Pleurotus ostreatus were used for isolation of polysaccharides. ► Three fractions were obtained by cold water, hot water and hot NaOH extractions. ► PC, PH and PB were α-(1-3),(1-6)-galactan, α-(1-4)-glucan and β-(1-3),(1-6)-glucan. ► Their molecular masses were 10kDa (PC), 140kDa (PH) and 30kDa (PB). ► All the polysaccharides displayed a linear backbone.
Novel water-soluble polysaccharides have been isolated from the fruiting bodies of the edible mushroom Pleurotus ostreatus. Three polysaccharide fractions were obtained by ethanol precipitation from cold water, hot water and hot aqueous NaOH extracts. The fractions were purified by size exclusion chromatography showing a unique carbohydrate occurring in each fraction: PC from the cold fraction, PH from the hot fraction and PB from the hot aqueous NaOH fraction. The analysis of the methylated alditol acetates and the NMR studies revealed that all the polysaccharides displayed a linear backbone. PC was formed by α-(1→3),(1→6)-linked galactopyranosyl residues whereas PH and PB consisted of glucose-linked units. PH was exclusively composed of glucopyranosyl units bound by α-(1→4) linkages whereas PB was a β-linked glucan showing (1→3) and (1→6) glycosidic bonds. The analysis of molecular arrangement by complexation with Congo red showed that only the β-linked polysaccharide (PB) displayed a triple helix conformation. |
| doi_str_mv | 10.1016/j.carres.2012.06.016 |
| format | Article |
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► Fruiting bodies of Pleurotus ostreatus were used for isolation of polysaccharides. ► Three fractions were obtained by cold water, hot water and hot NaOH extractions. ► PC, PH and PB were α-(1-3),(1-6)-galactan, α-(1-4)-glucan and β-(1-3),(1-6)-glucan. ► Their molecular masses were 10kDa (PC), 140kDa (PH) and 30kDa (PB). ► All the polysaccharides displayed a linear backbone.
Novel water-soluble polysaccharides have been isolated from the fruiting bodies of the edible mushroom Pleurotus ostreatus. Three polysaccharide fractions were obtained by ethanol precipitation from cold water, hot water and hot aqueous NaOH extracts. The fractions were purified by size exclusion chromatography showing a unique carbohydrate occurring in each fraction: PC from the cold fraction, PH from the hot fraction and PB from the hot aqueous NaOH fraction. The analysis of the methylated alditol acetates and the NMR studies revealed that all the polysaccharides displayed a linear backbone. PC was formed by α-(1→3),(1→6)-linked galactopyranosyl residues whereas PH and PB consisted of glucose-linked units. PH was exclusively composed of glucopyranosyl units bound by α-(1→4) linkages whereas PB was a β-linked glucan showing (1→3) and (1→6) glycosidic bonds. The analysis of molecular arrangement by complexation with Congo red showed that only the β-linked polysaccharide (PB) displayed a triple helix conformation.</description><identifier>ISSN: 0008-6215</identifier><identifier>EISSN: 1873-426X</identifier><identifier>EISSN: 0008-6215</identifier><identifier>DOI: 10.1016/j.carres.2012.06.016</identifier><identifier>PMID: 22824506</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>acetates ; Carbohydrate ; Chemical Sciences ; cold ; Congo red ; ethanol ; Food and Nutrition ; fruiting bodies ; Fruiting Bodies, Fungal - chemistry ; Galactan ; Galactans - chemistry ; gel chromatography ; Glucan ; glucans ; Glucans - chemistry ; Life Sciences ; Molecular Weight ; Mushroom ; mushrooms ; nuclear magnetic resonance spectroscopy ; Pleurotus - chemistry ; Pleurotus ostreatus ; Polymers ; Polysaccharide ; Polysaccharides - chemistry ; sodium hydroxide ; Solubility</subject><ispartof>Carbohydrate research, 2012-09, Vol.358, p.72-77</ispartof><rights>2012 Elsevier Ltd</rights><rights>Copyright © 2012 Elsevier Ltd. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-cf6fadfa1e774ab8347ba95268c0a6e91aa0378959cf843c463d1812616b576c3</citedby><cites>FETCH-LOGICAL-c420t-cf6fadfa1e774ab8347ba95268c0a6e91aa0378959cf843c463d1812616b576c3</cites><orcidid>0000-0001-5441-7299</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0008621512002765$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22824506$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02652218$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Palacios, Irene</creatorcontrib><creatorcontrib>García-Lafuente, Ana</creatorcontrib><creatorcontrib>Guillamón, Eva</creatorcontrib><creatorcontrib>Villares, Ana</creatorcontrib><title>Novel isolation of water-soluble polysaccharides from the fruiting bodies of Pleurotus ostreatus mushrooms</title><title>Carbohydrate research</title><addtitle>Carbohydr Res</addtitle><description>[Display omitted]
► Fruiting bodies of Pleurotus ostreatus were used for isolation of polysaccharides. ► Three fractions were obtained by cold water, hot water and hot NaOH extractions. ► PC, PH and PB were α-(1-3),(1-6)-galactan, α-(1-4)-glucan and β-(1-3),(1-6)-glucan. ► Their molecular masses were 10kDa (PC), 140kDa (PH) and 30kDa (PB). ► All the polysaccharides displayed a linear backbone.
Novel water-soluble polysaccharides have been isolated from the fruiting bodies of the edible mushroom Pleurotus ostreatus. Three polysaccharide fractions were obtained by ethanol precipitation from cold water, hot water and hot aqueous NaOH extracts. The fractions were purified by size exclusion chromatography showing a unique carbohydrate occurring in each fraction: PC from the cold fraction, PH from the hot fraction and PB from the hot aqueous NaOH fraction. The analysis of the methylated alditol acetates and the NMR studies revealed that all the polysaccharides displayed a linear backbone. PC was formed by α-(1→3),(1→6)-linked galactopyranosyl residues whereas PH and PB consisted of glucose-linked units. PH was exclusively composed of glucopyranosyl units bound by α-(1→4) linkages whereas PB was a β-linked glucan showing (1→3) and (1→6) glycosidic bonds. The analysis of molecular arrangement by complexation with Congo red showed that only the β-linked polysaccharide (PB) displayed a triple helix conformation.</description><subject>acetates</subject><subject>Carbohydrate</subject><subject>Chemical Sciences</subject><subject>cold</subject><subject>Congo red</subject><subject>ethanol</subject><subject>Food and Nutrition</subject><subject>fruiting bodies</subject><subject>Fruiting Bodies, Fungal - chemistry</subject><subject>Galactan</subject><subject>Galactans - chemistry</subject><subject>gel chromatography</subject><subject>Glucan</subject><subject>glucans</subject><subject>Glucans - chemistry</subject><subject>Life Sciences</subject><subject>Molecular Weight</subject><subject>Mushroom</subject><subject>mushrooms</subject><subject>nuclear magnetic resonance spectroscopy</subject><subject>Pleurotus - chemistry</subject><subject>Pleurotus ostreatus</subject><subject>Polymers</subject><subject>Polysaccharide</subject><subject>Polysaccharides - chemistry</subject><subject>sodium hydroxide</subject><subject>Solubility</subject><issn>0008-6215</issn><issn>1873-426X</issn><issn>0008-6215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1v1DAQhi0EotuPf4Agx_aQ4K843gtSVQFFWgESrdSbNXEmXa-S9WI7i_rvcZTSIyfPvH7m9cgvIe8YrRhl6uOushACxopTxiuqqiy-IiumG1FKrh5ekxWlVJeKs_qEnMa4yy1VjXpLTjjXXNZUrcjuuz_iULjoB0jO7wvfF38gYSizMrUDFgc_PEWwdgvBdRiLPvixSFvMxeSS2z8Wre9cvsiTPwecgk9TbmIKCHM1TnEbvB_jOXnTwxDx4vk8I_dfPt_d3JabH1-_3VxvSis5TaXtVQ9dDwybRkKrhWxaWNdcaUtB4ZoBUNHodb22vZbCSiU6phlXTLV1o6w4I1eL7xYGcwhuhPBkPDhze70xs0a5qjln-sgye7mwh-B_TxiTGV20OAywRz9Fw6gQSnNOeUblgtrgYwzYv3gzauZEzM4siZg5EUOVyWIee__8wtSO2L0M_YsgAx8WoAdv4DG4aO5_ZQeV0xJSyHnJTwuB-deODoOJ1uHeYucC2mQ67_6_w18ltqjD</recordid><startdate>20120901</startdate><enddate>20120901</enddate><creator>Palacios, Irene</creator><creator>García-Lafuente, Ana</creator><creator>Guillamón, Eva</creator><creator>Villares, Ana</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0001-5441-7299</orcidid></search><sort><creationdate>20120901</creationdate><title>Novel isolation of water-soluble polysaccharides from the fruiting bodies of Pleurotus ostreatus mushrooms</title><author>Palacios, Irene ; García-Lafuente, Ana ; Guillamón, Eva ; Villares, Ana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-cf6fadfa1e774ab8347ba95268c0a6e91aa0378959cf843c463d1812616b576c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>acetates</topic><topic>Carbohydrate</topic><topic>Chemical Sciences</topic><topic>cold</topic><topic>Congo red</topic><topic>ethanol</topic><topic>Food and Nutrition</topic><topic>fruiting bodies</topic><topic>Fruiting Bodies, Fungal - chemistry</topic><topic>Galactan</topic><topic>Galactans - chemistry</topic><topic>gel chromatography</topic><topic>Glucan</topic><topic>glucans</topic><topic>Glucans - chemistry</topic><topic>Life Sciences</topic><topic>Molecular Weight</topic><topic>Mushroom</topic><topic>mushrooms</topic><topic>nuclear magnetic resonance spectroscopy</topic><topic>Pleurotus - chemistry</topic><topic>Pleurotus ostreatus</topic><topic>Polymers</topic><topic>Polysaccharide</topic><topic>Polysaccharides - chemistry</topic><topic>sodium hydroxide</topic><topic>Solubility</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Palacios, Irene</creatorcontrib><creatorcontrib>García-Lafuente, Ana</creatorcontrib><creatorcontrib>Guillamón, Eva</creatorcontrib><creatorcontrib>Villares, Ana</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Carbohydrate research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Palacios, Irene</au><au>García-Lafuente, Ana</au><au>Guillamón, Eva</au><au>Villares, Ana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel isolation of water-soluble polysaccharides from the fruiting bodies of Pleurotus ostreatus mushrooms</atitle><jtitle>Carbohydrate research</jtitle><addtitle>Carbohydr Res</addtitle><date>2012-09-01</date><risdate>2012</risdate><volume>358</volume><spage>72</spage><epage>77</epage><pages>72-77</pages><issn>0008-6215</issn><eissn>1873-426X</eissn><eissn>0008-6215</eissn><abstract>[Display omitted]
► Fruiting bodies of Pleurotus ostreatus were used for isolation of polysaccharides. ► Three fractions were obtained by cold water, hot water and hot NaOH extractions. ► PC, PH and PB were α-(1-3),(1-6)-galactan, α-(1-4)-glucan and β-(1-3),(1-6)-glucan. ► Their molecular masses were 10kDa (PC), 140kDa (PH) and 30kDa (PB). ► All the polysaccharides displayed a linear backbone.
Novel water-soluble polysaccharides have been isolated from the fruiting bodies of the edible mushroom Pleurotus ostreatus. Three polysaccharide fractions were obtained by ethanol precipitation from cold water, hot water and hot aqueous NaOH extracts. The fractions were purified by size exclusion chromatography showing a unique carbohydrate occurring in each fraction: PC from the cold fraction, PH from the hot fraction and PB from the hot aqueous NaOH fraction. The analysis of the methylated alditol acetates and the NMR studies revealed that all the polysaccharides displayed a linear backbone. PC was formed by α-(1→3),(1→6)-linked galactopyranosyl residues whereas PH and PB consisted of glucose-linked units. PH was exclusively composed of glucopyranosyl units bound by α-(1→4) linkages whereas PB was a β-linked glucan showing (1→3) and (1→6) glycosidic bonds. The analysis of molecular arrangement by complexation with Congo red showed that only the β-linked polysaccharide (PB) displayed a triple helix conformation.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>22824506</pmid><doi>10.1016/j.carres.2012.06.016</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0001-5441-7299</orcidid></addata></record> |
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| subjects | acetates Carbohydrate Chemical Sciences cold Congo red ethanol Food and Nutrition fruiting bodies Fruiting Bodies, Fungal - chemistry Galactan Galactans - chemistry gel chromatography Glucan glucans Glucans - chemistry Life Sciences Molecular Weight Mushroom mushrooms nuclear magnetic resonance spectroscopy Pleurotus - chemistry Pleurotus ostreatus Polymers Polysaccharide Polysaccharides - chemistry sodium hydroxide Solubility |
| title | Novel isolation of water-soluble polysaccharides from the fruiting bodies of Pleurotus ostreatus mushrooms |
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